Electroosmotic Flow Research Articles

Sensitivity analysis on electro-osmotic flow of EMHD tangent hyperbolic nanofluid through porous rotating disk with variable thermal conductivity, Stefan blowing and thermal radiation

Sensitivity analysis on electro-osmotic flow of EMHD tangent hyperbolic nanofluid through porous rotating disk with variable thermal conductivity, Stefan blowing and thermal radiation

Interface dynamics in electroosmotic flow systems with non-Newtonian fluid frontiers

Abstract Microfluidic applications involving liquid manipulation, selective membranes, and energy harvesting strongly em-phasize the importance of the electrokinetic phenomenon, which is widely used at multiple fluid and electrochemi-cal interfaces. However, critical scientific issues that address multifield coupling and multiscale physics have not been well addressed in non-Newtonian fluids. In this paper, electrical field–fluid flow–ion transport coupling is numerically implemented in two mainstream problems, i.e., induced electroconvection phenomena at ion-selective interfaces and induced charge electroosmosis in polarized cylinders. The effects of different non-Newtonian rheo-logical properties, which are absent in Newtonian fluids, on the interfacial dynamics, instability and ion transport are examined. The results reveal that the non-Newtonian rheology significantly modulates the statistical data and interfacial phenomena. Generalized power-law fluids alter velocity and interfacial charge profiles, with shear thin-ning enhancing ion transport to lower overlimiting current thresholds and shear thickening broadening the limiting current region (with hindered ion transport). In Boger-type Oldroyd-B fluids, the addition of polymer decreases the velocity amplitude and increases the interface resistance. At low voltages, polymer viscoelasticity minimally affects the ohmic and limiting regions, but under convection-dominated flow, different rheological parameters, such as the viscosity ratio, Weissenberg number, anisotropic parameter, and electrohydrodynamic coupling con-stants, enable controllable regulation of ion transport behavior across a wide range. Finally, this paper states that modulated electroosmosis by complex charged polymers is the future cutting edge. The relevant results supple-ment the non-Newtonian physics of electrokinetic systems and provide guidance for the design and operation of microfluidic devices.

Pulse electroosmotic flow of Newtonian fluids in parallel plate microchannels under triangular and half-sinusoidal pulse electric fields

Unlike the conventional electroosmotic flow (EOF) driven by direct current and alternating current electric fields, this study investigates the pulse EOF of Newtonian fluids through a parallel plate microchannel actuated by pulse electric fields. Specifically, the pulses considered encompass triangular and half-sinusoidal pulse waves. By applying the Laplace transform method and the residual theorem, the analytical solutions for the velocity and volumetric flow rate of the pulse EOF associated with these two pulse waves are derived, respectively. The influence of pulse width a¯ and electrokinetic width K on velocity is further considered, while the volumetric flow rate as a function of time t¯ and electrokinetic width K is examined separately. A comparison of the volumetric flow rates related to these two pulse waves under varying parameters is also conducted. The research findings indicate that irrespective of the pulse wave, a broader pulse width results in a prolonged period and increased amplitude of the velocity profile. Elevating the electrokinetic width yields higher near-wall velocities, with negligible effect on near-center velocities. It is noteworthy that regardless of the electrokinetic width, the near-wall velocity exceeds that of the near-center during the first half-cycle, while the situation reverses during the second half-cycle. The volumetric flow rate varies periodically with time, initially surging rapidly with electrokinetic width before gradually stabilizing at a constant level. More interestingly, independent of pulse width and electrokinetic width, the volumetric flow rates linked to the half-sinusoidal pulse wave consistently surpass those of the triangular pulse wave. For any pulse width, the volumetric flow rates corresponding to the two pulse waves grow with higher electrokinetic widths, especially prominent at alternating intervals of the two half-cycles within a complete cycle. These findings have important implications for improving the design and optimization of microfluidic devices in engineering and biomedical applications utilizing pulse EOF.

Graphite-Based Bio-Mimetic Nanopores for Protein Sequencing and Beyond.

Protein sequencing using nanopores represents the next frontier in bio-analytics. However, linearizing unfolded proteins and controlling their translocation speed through solid-state nanopores pose significant challenges in protein sequencing. In order to address these issues, this work proposes a biomimetic graphite-based nanopore construction. These nanopores feature a nanometer-sized pore with a constriction zone, mimicking the structure of the α-hemolysin protein pore. Ourall-atom Molecular Dynamics simulations demonstrate the high practical potential of these nanopores by revealing how their charge state renders them complete ion-selective and generates an electro-osmotic flow. This study shows that this nanopore construction can detect peptides at the single amino acid level by analyzing the ionic current traces generated as peptides traverse the nanopore. The novelty of the proposed nanopore lies in its ability to modulate the hydrodynamic drag induced by electro-osmotic flow, relative to the electro-phoretic force. This investigation reveals that tuning these forces helps to linearize translocating peptides and extend the residence time of individual amino acids at the constriction zone of the pore. This significantly enhances the detection and sequencing efficiency of the pore. Furthermore, the high relevance of the proposed nanopores is underscored for seawater desalination through electrodialysis and extends to ion separation under salinitygradients.

Effects of electric and magnetic fields in magnetic mixing in electroosmotic flows

In this paper, mixing efficiency in electroosmotic flow is numerically simulated at the electric double layer region in the presence of a magnetic field. An incompressible and laminar model is adopted to numerically solve the governing equations involving the magnetic field, electric potential fields, concentration distribution for positive and negative ions (Nernst–Planck), and species concentration. To validate the numerical study, an ideal electroosmotic flow with charged walls is simulated and the results are compared with the analytical solution. For the case with a Reynolds number of 0.02, microchannel length of 40 μm, the results show that by applying a magnetic field, the mixing efficiency along the microchannel length increases from 60.5% to 79.67%. It is found that the existence of a magnetic field in the electric double layer has a significant impact on pressure distribution along the microchannel wall, however, its effects on the electric fields (internal and external), the distribution of positive and negative ions, and the net electrical charge density are marginal. In addition, the presence of magnetic field creates two relatively large vortices inside the microchannel. The outcomes of the present study will help to improve mixing efficiency in micro-devices with applications in micro-analysis systems and lab-on-a-chip instruments.

Electro-osmotic flow and heat transfer in Jeffery fluid: A multi-membrane microchannel model

Microscale heat transfer is vital for the performance of smart thermal devices like heat sinks, thermosyphons, and microheat pipes. This study introduces a biothermal pumping flow model based on a multi-membrane pumping mechanism that leverages microscale heat transfer. The model describes rhythmic contraction and relaxation of membranes, combined with electro-osmosis in Jeffery fluid flow within a vertical microchannel of finite length. Two membranes on the microchannel walls, with varying amplitudes, diameters, and phase lags, generate pressure that moves fluid in both directions through contraction and expansion cycles. The model is based on the conservation of mass and momentum, using a low Reynolds number approximation to capture microscale transport phenomena at biomedical scales. Dimensionless conservation equations are analytically solved under no-slip boundary conditions, with results computed in MATLAB for clarity. Axial velocity results are simulated and verified using the optimal homotopy analysis method. The model explores the influence of key parameters (UHS, me, λ, Gr, β) on pressure gradient, velocity distribution, volumetric flow rates, skin friction, Nusselt number, and stream function. The findings demonstrate that pressure from membrane motion is significantly affected by thermal effects and buoyancy forces, and flow and pumping characteristics are largely determined by the fluid's rheological qualities and the geometrical features of the membrane. This study provides novel ideas for enhancing the functionality and design of smart thermal devices while also advancing microscale heat transfer technology.

Investigating the effect of finite ionic size and solvent polarization on induced charge electro-osmosis around a perfectly polarizable cylinder

Many industrially relevant microfluidic applications use concentrated solutions of macro-molecular solutes dissolved in polar solvents like water, which are typically deployed at high voltages. In this study, we investigate the effect of finite ionic sizes and solvent polarization on induced charge electro-osmotic flow around a perfectly polarizable cylinder, at high electric field strengths and ionic concentrations. The flow is actuated by means of a direct current electric field, and the step response of various flow parameters are studied numerically. Finite ionic sizes, defined through a steric factor ν, are modeled using the modified Poisson–Nernst–Planck model. Additionally, a field-dependent permittivity, characterized by a solvent polarization number A, accounts for molecular re-orientation effects. Our findings reveal an ion-size modulated decrement in charge concentration in the electrical double layer and an augmentation in the electric field. Remarkably, the resulting flow velocities increase with ion size. Solvent polarization, on the other hand, results in a marked reduction in flow velocities. Steric effects, however, dominate over a large range of parameter space (applied voltage and bulk ionic concentration) as compared to solvent polarization. Finally, we demonstrate that unequal ionic sizes result in flow asymmetries at the steady-state, thereby generating net electro-phoretic motion of suspended particles.

Single-Molecule-Based, Label-Free Monitoring of Molecular Glue Efficacies for Promoting Protein-Protein Interactions Using YaxAB Nanopores.

Modulating protein-protein interactions (PPIs) is an attractive strategy in drug discovery. Molecular glues, bifunctional small-molecule drugs that promote PPIs, offer an approach to targeting traditionally undruggable targets. However, the efficient discovery of molecular glues has been hampered by the current limitations of conventional ensemble-averaging-based methods. In this study, we present a YaxAB nanopore for probing the efficacy of molecular glues in inducing PPIs. Using YaxAB nanopores, we demonstrate single-molecule-based, label-free monitoring of protein complex formation between mammalian target of rapamycin (mTOR) and FK506-binding proteins (FKBPs) triggered by the molecular glue, rapamycin. Owing to its wide entrance and adjustable pore size, in combination with potent electro-osmotic flow (EOF), a single funnel-shaped YaxAB nanopore enables the simultaneous detection and single-molecule-level quantification of multiprotein states, including single proteins, binary complexes, and ternary complexes induced by rapamycin. Notably, YaxAB nanopores could sensitively discriminate between the binary complexes or ternary complexes induced by rapamycin and its analogues, despite the subtle size differences of ∼122 or ∼116 Da, respectively. Taken together, our results provide proof-of-concept for single-molecule-based, label-free, and ultrasensitive screening and structure-activity relationship (SAR) analysis of molecular glues, which will contribute to low-cost, highly efficient discovery, and rational design of bifunctional modality of drugs, such as molecular glues.

Two-Layer Electroosmotic Flow in a Parallel Plate Microchannel with Sinusoidal Corrugation

This study investigates the electroosmotic flow (EOF) of a two-layer Newtonian fluid system in a parallel plate microchannel with sinusoidal corrugated walls. The upper fluid is conducting, while the lower fluid is nonconducting. This analysis is performed under the Debye–Hückel approximation, utilizing perturbation expansion and the separation of variables. The potential distribution, velocity field, and the dependence of average velocity on roughness are derived. It is observed that the velocity distribution w(x, y), is significantly influenced by the phase difference θ between the corrugations on the upper and lower walls. The velocity w(x, y) decreases with an increase in the viscosity ratio μr of the bottom to top fluid, and w(x, y) is directly proportional to the dimensionless pressure gradient G and the zeta potential ratio ζ. The variation of the average velocity increment (roughness function) u2m related to wall roughness tends to decrease with the increase of the corrugation wave number λ, the electrokinetic width K, the depth ratio hr of the bottom to top fluid, the zeta potential ratio ζ and the dimensionless pressure gradient G; and increases with the increase of the viscosity ratio μr of the bottom to top fluid. Furthermore, the effect of uI2m is smaller than that of uII2m.

Optimizing electrokinetic remediation for pollutant removal and electroosmosis/dewatering using lateral anode configurations

Soil electrokinetics (SEK) research has been widely used in various fields such as soil remediation, dewatering, land restoration, geophysics, sedimentation, pollution prevention, consolidation, and seed germination. According to our most recent published research on SEK process design modifications during the last 30 years (1993–2022), more than 150 designs have been introduced to assure SEK’s maximum performance. Incorporating lateral electrodes/anodes was not documented in the existing literature, which motivated us to investigate the output of this design. In this study, we aimed to enhance the performance of the perforated cathode pipe soil electrokinetic remediation (SEKR) system (PCPSS) for removing inorganic pollutants by installing lateral anodes (LA-PCPSS) using two approaches. In the first approach, the LA-PCPSS was connected to different sources of applied voltages (DSAV) from different power supplies, while in the second approach, the entire operation system was connected to the same source of applied voltage (SSAV). We used the Taguchi approach (L9OA) to determine the optimal levels of applied voltages for the DSAV system. The results indicated that the DSAV-(LA-PCPSS) could be optimized at an applied voltage of 1 V cm−1 for the surface and the first and second lateral anodes. The indigenous Sr (elements found in the tested soil without artificial pollution) in kaolinite showed the best response among other elements (Ni and other indigenous elements) when optimizing the DSAV-(LA-PCPSS) using the Taguchi approach. Installing lateral anodes (position B) supplied to low applied voltage (0.5 V cm−1) improved the electroosmosis (EO) rate/dewatering. Reverse migration of ions was observed during the remediation of real contaminated soil using the SSAV-(LA-PCPSS). The DSAV-(LA-PCPSS) is considered an appropriate design for the SEKR of inorganic pollutants, and increases the EO flow/dewatering. Additionally, the increased energy consumption employing the DSAV-(LA-PCPSS) was extremely minimal compared to the traditional PCPSS, which is an economic advantage for SEKR research. The DSAV-(LA-PCPSS) is still under optimization/intensification process, and subsequent processes will be examined to achieve high efficiency.

Controlled Translocation of Proteins through a Biological Nanopore for Single-Protein Fingerprint Identification.

After the successful sequencing of nucleic acids, nanopore technology has now been applied to proteins. Recently, it has been demonstrated that an electro-osmotic flow can be used to induce the transport of unraveled polypeptides across nanopores. Polypeptide translocation, however, is too fast for accurate reading its amino acid compositions. Here, we show that the introduction of hydrophobic residues into the lumen of the nanopore reduces the protein translocation speed. Importantly, the introduction of a tyrosine at the entry of the nanopore and an isoleucine at the entry of the β-barrel of the nanopore reduced the speed of translocation to ∼10 amino acids/millisecond while keeping a relatively large ionic current, a crucial component for protein identification. These nanopores showed unique features within their current signatures, which may pave the way toward protein fingerprinting using nanopores.

Heat and mass transfer analysis of s-PTT nanofluid in microchannels under combined electroosmotic and pressure-driven flows with wall slip using the homotopy perturbation method

The heat and mass transfer of the electroosmotic flow in microchannel transporting viscoelastic nanofluid is investigated considering Brownian motion of nanoparticles and slip boundary conditions. The simplified Phan-Thien-Tanner model is employed to describe the rheological behavior of fluid and the nonlinear Navier model with non-zero slip critical shear stress is considered at walls. The governing nonlinear momentum, mass, and heat transfer equations are solved using the Homotopy Perturbation Method. The study reveals that increasing the fluid elasticity, nanoparticle concentration, and size significantly enhances the flow rate, heat and mass transfer. Additionally, elasticity and Reynolds number decrease the friction factor. Reducing the double-layer thickness and increasing the Reynolds number lead to higher flow rates and fluid velocities. Notably, the findings emphasize the critical role of the slip conditions on the Sherwood and Nusselt numbers.

Electroosmotic peristaltic transport of magnetohydrodynamic Casson nanofluid in a non-uniform wavy porous asymmetric micro-channel

Magnetohydrodynamics (MHD) have numerous engineering and biomedical applications such as sensors, MHD pumps, magnetic medications, MRI, cancer therapy, astronomy, cosmology, earthquakes, and cardiovascular devices. In view of these applications and current developments, we investigate the magnetohydrodynamic MHD electro-osmotic flow of Casson nanofluid during peristaltic movement in a non-uniform porous asymmetric channel. The effect of thermal radiation, heat source, and Hall current on the Casson fluid peristaltic pumping in a porous medium is taken into consideration. The effect of chemical reactions is also considered. The mass, momentum, energy, and concentration equations were constructed using the proper transformations and dimensionless variables to make them easier for non-Newtonian fluids. A lubricating strategy is used to make the system less complicated. The Boltzmann distribution of electric potential over an electric double layer is studied using the Debye–Huckel approximation. The temperature and concentration equations are addressed using the homotopy perturbation method (HPM), while the exact solution is determined for the velocity field. The study examines the performance of velocity, pressure rise, temperature, concentration, streamlines, Nusselt, and Sherwood numbers for the involved parameters using graphical illustrations and tables. Asymmetric channels exhibit varying behavior, with velocity declining near the left wall and accelerating towards the right wall while enhancing the Casson fluid parameter. The pumping rate boosts in the retrograde region due to the evolution of the permeability parameter value, while it declines in the augment region. The temperature profile optimizes as the value of the heat source parameter gets higher. The concentration profile significantly falls as the chemical reaction parameter rises. The size of the trapped bolus strengthens with a spike in the parameter for the Casson fluid.

A perspective on guided electrophoretic transport of particles in liquid crystals

Nonlinear electrophoresis in complex fluids like nematic liquid crystals provides new pathways toward achieving precisely controlled motion and assembly of microscopic objects. The nematic host introduces a paradigm shift in the mechanism of electrophoretic transport by generating unbalanced electro-osmotic flows around the colloidal particle due to symmetry breaking of the medium caused by the induced topological defects. Rationally designed particles, which induce various types of defects and asymmetries, provide new opportunities in this regard. In this Perspective article, we discuss how the asymmetry in the shape and interfacial properties help in piloting the particles using an AC electric field. Finally, we propose some feasible strategies to achieve navigational control using magnetic and photo-responsive particles, guided by orthogonal electric, magnetic fields, and light, respectively.

Simulation study on mitigating permeate flux decline with electroosmotic flux in dead-end ultrafiltration process

Piezoelectric membranes establish electrokinetic phenomena, including electroosmotic flow, to mitigate membrane fouling in the ultrafiltration process. This study employs numerical simulations to evaluate the role and impact of the electroosmotic flux effect on permeate flux decline and fouling mechanisms in the dead-end filtration process for silicon dioxide particles. This research demonstrates that electroosmotic flux significantly alleviates flux decline across varying values of surface zeta potential, membrane porosity, particle size and inlet concentration. Specifically, the electroosmotic flux effect is most pronounced in reducing flux decline when the surface zeta potential is higher and the membrane structure is more porous. This reduction in flux decline is attributed to the enhanced lift and drag forces generated by electroosmotic flux near the electrical double layer, which prevents rapid initial adhesion and subsequent fouling, such as internal blocking and cake layer formation. Additionally, the electroosmotic flux effect is most effective with smaller particle sizes and higher feed concentrations, helping to mitigate flux decline that would otherwise occur in the absence of electroosmotic flux. Compared to experimental and modelling investigations, the electroosmotic flux effect is shown to complement a reduction in permeate flux drop to facilitate the longevity of membranes in the ultrafiltration process.

DC Electric Fields Promote Biodegradation of Waterborne Naphthalene in Biofilter Systems.

Biofiltration is a simple and low-cost method for the cleanup of contaminated water. However, the reduced availability of dissolved chemicals to surface-attached degrader bacteria may limit its efficient use at certain hydraulic loadings. When a direct current (DC) electric field is applied to an immersed packed bed, it invokes electrokinetic processes, such as electroosmotic water flow (EOF). EOF is a surface-charge-induced plug-flow-shaped movement of pore fluids. It acts at a nanometer distance above surfaces and allows the change of microscale pressure-driven flow profiles and, hence, the availability of dissolved contaminants to microbial degraders. In laboratory percolation columns, we assessed the effects of a weak DC electric field (E = 0.5 V·cm-1) on the biodegradation of waterborne naphthalene (NAH) by surface-attached Pseudomonas fluorescens LP6a. To vary NAH bioavailability, we used different NAH concentrations (C0 = 2.7, 5.1, or 7.8 × 10-5 mol·L-1) and Darcy velocities typical for biofiltration ( = 0.2-1.2 × 10-4 m·s-1). In DC-free controls, we observed higher specific degradation rates (qc) at higher NAH concentrations. The qc depended on , suggesting bioavailability restrictions depending on the hydraulic residence times. DC fields consistently increased qc and resulted in linearly increasing benefits up to 55% with rising hydraulic loadings relative to controls. We explain these biodegradation benefits by EOF-altered microscale flow profiles allowing for better NAH provision to bacteria attached to the collectors even though the EOF was calculated to be 100-800 times smaller than bulk water flow. Our data suggest that electrokinetic approaches may give rise to future technical applications that allow regulating biodegradation, for example, in response to fluctuating hydraulic loadings.

Electro-osmotic flow instability of viscoelastic fluids in a nanochannel

The study of the complex rheological properties of viscoelastic fluids in nanochannels will facilitate the application of nanofluidics in biomedical and other fields. However, the flow of viscoelastic fluids in nanochannels has significant instabilities, and numerical simulation failures are prone to occur at high Weissenberg numbers (Wi). In this study, the simplified Phan-Thien-Tanner viscoelastic fluid model is solved using the log-conformation tensor approach, and the effects of rheological parameters of the viscoelastic fluid, such as the Weissenberg number (Wi), extensibility parameter (ε), and viscosity ratio (β), on the flow characteristics and flow instability within the nanochannel are investigated. The results indicate that the variation of rheological parameters of viscoelastic fluids has a significant effect on the flow state and flow instability of fluids in nanochannels. When the rheological parameters are in a specific range, the flow velocity and outlet current in the nanochannel exhibit relatively regular periodic fluctuations. As the flow transitions from an up-and-down moving single-vortex state to a symmetric double-vortex state, the average velocity of the central axis in the nanochannel is increased by about 15%. Furthermore, when Wi increases from 150 to 400, the length and height of the vortex increase by 50% and 100%, respectively.

Effect of charge inversion on the electrokinetic transport of nanoconfined multivalent ionic solutions

Understanding the effects of phenomena occurring at electrically charged interfaces, such as charge inversion (CI), is crucial for enabling electroosmosis as an efficient transport mechanism in nanodevices. Here, we employ molecular dynamics (MD) simulations to systematically analyze the effect of CI on the electrokinetic transport of multivalent ionic solutions confined in amorphous silica nanochannels. We employ mixtures of monovalent and multivalent counterions while fixing the total ionic concentration to establish correlations between observed phenomena and the amount of multivalent ionic species in the electrolyte solution. The results show that the development of CI is related to a decrease in the mobility of the fluid layers adjacent to the charged surface. In addition, we observe that interfacial overcharging disrupts the water molecular orientation in the fluid layers adjacent to the channel walls. From the non-equilibrium MD simulations of electro-osmotic flow, we disclose the influence of phenomena related to the presence of CI. In particular, flow reversal occurs in scenarios involving CI due to increased local viscosity and a higher concentration of coions within the hydrodynamically mobile and electrokinetically active region of the charged interface. We also find that the magnitude of the wall zeta (ζ) potential displays a monotonic increase with the development of CI in the system. Moreover, we explain why positioning the wall ζ potential at an imaginary (slip) plane, which separates the hydrodynamically mobile and immobile fluid, is misleading.

Protein denaturation inspired microchannel-based electrochemiluminescence sensor for formaldehyde detection

Establishing an effective system to measure formaldehyde (HCHO) content in food is of great significance due to food safety concern. Inspired by the mechanism of HCHO-induced protein denaturation and its effect on ion/molecule transport in nanochannels, a bioinspired microchannel-based electrochemiluminescence (ECL) sensor was constructed for HCHO detection. Benefiting from the water solubility of HCHO, the molecules rapidly spread and enriched at the ethylenediamine (EDA) functionalized microchannel interface. The reaction between EDA and HCHO significantly increased the negative charge density, leading to enhanced electroosmotic flow (EOF). This enhancement resulted in ion concentration depletion at the microchannel tip and a corresponding decrease in ionic current and ECL intensity. The ECL intensity exhibited a linear dependence on the logarithm of HCHO concentration ranging from 1 pg mL−1 to 100 ng mL−1, with a detection limit of 0.26 pg mL−1(S/N = 3). The biosensor demonstrated high selectivity, successfully detecting HCHO in shrimp samples. The performance of the bioinspired sensor was confirmed through comparation with existing methods, showcasing its superior sensitivity and reliability. The bioinspired sensor provides robust technical support for HCHO detection, crucial for food safety monitoring. Additionally, the innovative combination of bionics and microchannel-based ECL technology broadens the application range of ECL sensors, marking a significant advancement in the field.

Preparation of DNA nanoflower-modified capillary silica monoliths for chiral separation.

Innovative chiral capillary silica monoliths (CSMs) were developed based on DNA nanoflowers (DNFs). Baseline separation of enantiomers such as atenolol, tyrosine, histidine, and nefopam was achieved by using DNF-modified CSMs, and the obtained resolution value was higher than 1.78. To further explore the effect of DNFs on enantioseparation, different types of chiral columns including DNA strand containing the complementary sequence of the template (DCT)-modified CSMs, DNF2-modified CSMs, and DNF3-modified CSMs were prepared as well. It was observed that DNF-modified CSMs displayed better chiral separation ability compared with DCT-based columns. The intra-day and inter-day repeatability of model analytes' retention time and resolution kept desirable relative standard deviation values of less than 8.28%. DNF2/DNF3-modified CSMs were able to achieve baseline separation of atenolol, propranolol, 2'-deoxyadenosine, and nefopam enantiomers. Molecular docking simulations were performed to investigate enantioselectivity mechanisms of DNA sequences for enantiomers. To indicate the successful construction of DNFs and DNF-modified CSMs, various charaterization approaches including scanning electron microscopy, agarose gel electrophoresis, dynamic light scattering analysis, electroosmotic flow, and Fourier-transform infrared spectroscopy were utilized. Moreover, the enantioseparation performance of DNF-modified CSMs was characterized in terms of sample volume, applied voltage, and buffer concentration. This work paves the way to applying DNF-based capillary electrochromatography microsystems for chiral separation.