Shear Wave Elasticity Research Articles

Analysis of the Effective Dynamic Properties of Particulate Composites with Respect to Constituent Properties

The propagation of longitudinal and shear elastic waves through a multi-phase material was studied and the effective elastic properties of the medium were evaluated. The distribution of the reinforcing inclusions was considered random throughout the matrix. The effective dynamic properties of the composites, including their effective bulk and shear moduli and effective densities, were examined along with the effective phase velocity and attenuation of the incident P and S waves. The Sabina–Willis model was employed to study the wave propagation behavior, and the model performance was analyzed through comparison with experimental data from the literature. The results indicated that wave propagation significantly depended on the physical and mechanical properties of inclusions relative to those of the matrix and the normalized wave number of the propagated elastic wave. Moreover, despite the fact that the elastic properties of the incidence in the P and S waves exhibited a similar trend, their values differed significantly. The results can serve as a design criterion for composite materials under dynamic loading.

Structural, electronic and elastic properties of FeCrAs Half-metallic ferromagnetic Half-Heusler Alloy: A First-Principles study

In this work, we employ full potential linearized augmented plane wave approach based on the density functional theory to calculate the structural, electronic, and elastic properties of FeCrAs Half-Heusler compound. For electron exchange, we have considered the most common Generalized Gradient Approximation (PBE-GGA and PBEsol-GGA), as exchange and correlation potential for investigating these properties. The half-metallic ferromagnetic behavior is confirmed by density of states and spin-polarized band structures analysis, which show that the FeCrAs has an indirect band-gap in the spin-down channel and a metallic behaviour in the spin-up channel. Furthermore, the elastic constants (Cij) and the related elastic moduli confirm their stability in the cubic phase and demonstrate their brittle nature. The Debye’s temperature along with compressional, Shear and average elastic wave velocities has also been calculated.[copyright information to be updated in production process]

Full Characterization of in vivo Muscle as an Elastic, Incompressible, Transversely Isotropic Material Using Ultrasonic Rotational 3D Shear Wave Elasticity Imaging.

Using a 3D rotational shear wave elasticity imaging (SWEI) setup, 3D shear wave data were acquired in the vastus lateralis of a healthy volunteer. The innate tilt between the transducer face and the muscle fibers results in the excitation of multiple shear wave modes, allowing for more complete characterization of muscle as an elastic, incompressible, transversely isotropic (ITI) material. The ability to measure both the shear vertical (SV) and shear horizontal (SH) wave speed allows for measurement of three independent parameters needed for full ITI material characterization: the longitudinal shear modulus μL , the transverse shear modulus μT , and the tensile anisotropy χE . Herein we develop and validate methodology to estimate these parameters and measure them in vivo, with μL = 5.77±1.00 kPa, μT = 1.93±0.41 kPa (giving shear anisotropy χμ = 2.11±0.92 ), and χE = 4.67±1.40 in a relaxed vastus lateralis muscle. We also demonstrate that 3D SWEI can be used to more accurately characterize muscle mechanical properties as compared to 2D SWEI.

Controllable Angle Shear Wavefront Reconstruction Based on Image Fusion Method for Shear Wave Elasticity Imaging.

The generation and measurement of shear waves are critical in ultrasonic elasticity imaging. Generally, the resulting wavefront direction is very important for accurately measuring the shear speed and estimating the medium elasticity. In this article, the proposed method can generate a compound shear wavefront with the same direction as speed reconstruction and zero angles between the wavefront and the focus direction, which can improve the estimation accuracy of shear wave velocity. Also, this method, called time-division multipoint excitation image fusion (TDMPEIF), can reconstruct the shear wave propagation images acquired at different depths of a medium according to the frame sequence to produce the shear waves front with a regulable angle. Moreover, the shear wave speed and the elasticity of a medium can be mapped quantitatively with this method. The results demonstrate that the TDMPEIF can improve the quality of the shear wave velocity images, which has wide application value and good promotion prospects for quantitative evaluation of tissue elasticity.

Intra- and inter-observer agreement of cervical shear wave elasticity imaging for induction of labor

The Bishop score poorly predicts the chance of vaginal birth after labor induction, perhaps because it requires subjective assessment. While shear wave speed (SWS) in an objective measure of tissue stiffness, studies conflict on its clinical value. However, bias and variance of measurements in vivo has not been fully evaluated. Our objective was to determine intra- and inter-observer agreement of SWS estimates in pregnant patients and identify sources of estimate bias.

Shear Wave Elasticity Imaging Using Nondiffractive Bessel Apodized Acoustic Radiation Force.

The acoustic radiation force impulse (ARFI) has been widely used in transient shear wave elasticity imaging (SWEI). For SWEI based on focused ARFI, the highest image quality exists inside the focal zone due to the limitation of the depth of focus and diffraction. Consequently, the areas outside the focal zone and in the near field present poor image quality. To address the limitations of the focused beam, we introduce Bessel apodized ARFI that enhances image quality and improves the depth of focus. The objective of this study is to evaluate the feasibility of SWEI based on Bessel ARF in simulation and experiment. We report measurements of elastogram image quality and depth of field in tissue-mimicking phantoms and ex vivo liver tissue. Our results demonstrate improved depth of field, image quality, and shear wave speed (SWS) estimation accuracy using Bessel push beams. As a result, Bessel ARF enlarges the field of view of elastograms. The signal-to-noise ratio (SNR) of Bessel SWEI is improved 26% compared with focused SWEI in homogeneous phantom. The estimated SWS by Bessel SWEI is closer to the measured SWS from a clinical scanner with an error of 0.3% compared to 2.4% with a focused beam. In heterogeneous phantoms, the contrast-to-noise ratios (CNRs) of shallow and deep inclusions are improved by 8.79 and 3.33 dB, respectively, under Bessel ARF. We also compare the results between Bessel SWEI and supersonic shear imaging (SSI), and the SNR of Bessel SWEI is improved by 8.1%. Compared with SSI, Bessel SWEI shows more accurate SWS estimates in high stiffness inclusions. Finally, Bessel SWEI can generate higher quality elastograms with less energy than conventional SSI.

Efficacy of shear wave elasticity for evaluating myocardial hypertrophy in hypertensive rats

Shear wave (SW) imaging is a novel ultrasound-based technique for assessing tissue characteristics. SW elasticity may be useful to assess the severity of hypertensive left ventricular (LV) hypertrophy. This study aimed to evaluate the efficacy of SW elasticity for assessing the degree of myocardial hypertrophy using hypertensive rats. Rats were divided into hypertension group and control group. SW elasticity was measured on the excised heart. Myocardial hypertrophy was assessed histologically. LV weight was greater in hypertension group. An increase in interventricular septum and LV free wall thicknesses was observed in hypertension group. SW elasticity was significantly higher in hypertension group than in control group (14.6 ± 4.3 kPa vs. 6.5 ± 1.1 kPa, P < 0.01). The cross-sectional area of cardiomyocytes was larger in hypertension group than in control group (397 ± 50 μm2 vs. 243 ± 14 μm2, P < 0.01), and SW elasticity was positively correlated with the cross-sectional area of cardiomyocytes (R = 0.96, P < 0.01). This study showed that SW elasticity was higher in hypertensive rats and was closely correlated with the degree of myocardial hypertrophy, suggesting the efficacy of SW elasticity for estimating the severity of hypertensive LV hypertrophy.

Development and evaluation of ligament phantoms targeted for shear wave tensiometry

Developing a shear wave tensiometer capable of non-invasively measuring ligament tension holds promise for enhancing research and clinical assessments of ligament function. Such development would benefit from tunable test specimens fabricated from well-characterized and consistent materials. Although previous work found that yarn can replicate the mechanical behavior of collateral ligaments, it is not obvious whether yarn-based phantoms would be suitable for development of a shear wave tensiometer for measuring ligament tension. Accordingly, the primary objective of this study was to characterize the mechanical properties and shear wave speed – stress relationships of ligament phantoms fabricated from yarn and silicone, and compare these results to published data from biological ligaments. We measured the mechanical properties and shear wave speeds during axial loading in nine phantoms with systematically varied material properties. We performed a simple linear regression between shear wave speed squared and axial stress to determine the shear wave speed – stress relationship for each phantom. We found comparable elastic moduli, hysteresis, and shear wave speed squared – stress regression parameters between the phantoms and collateral ligaments. For example, the ranges of the coefficients of determination (R2) and slopes across the nine phantoms were 0.84–0.95, and 0.78–1.27 kPa/m2/s2, respectively, which overlapped with the ranges found in a prior study in porcine collateral ligaments (0.84–0.996 and 0.34–1.18 kPa/m2/s2, respectively). Additionally, the shear wave speed squared – stress regression parameters varied predictably with the density of the phantom and the shear modulus of the silicone. In summary, we found that yarn-based phantoms serve as mechanical analogs for ligaments (i.e., are ligament mimicking), and thus, should prove beneficial for investigations into ligament structure-function relationships and in the development of a shear wave tensiometer for measuring ligament tension.

Correlation of elastic characteristics of breast lesions with the expression of Smad2/3.

BackgroundTo analyze the relationship between elastic characteristics and the expression of Smad2/3 in breast lesions.MethodsBetween April 2018 and October 2018, 135 lesions from 130 patients who underwent shear wave elastography before surgical excision or biopsy were included in the study. The shear wave elasticity features of the lesions, expression of Smad2/3 of the specimens, and their combined diagnostic efficacy was analyzed.ResultsOf the 135 lesions, 51 were malignant and 84 were benign. The elasticity ratio of lesions to peripheral parenchyma, maximum elasticity, mean elasticity, prevalent rate of “stiff rim sign”, and the expression level of Smad2/3 in the malignant pathological changes were obviously superior to those with benign pathological change (P<0.001). The Smad2/3 expression level had a positive correlation with the maximum, average elasticity, and the elastic ratio of lesions to peripheral parenchyma (r=0.657, 0.640, and 0.470, respectively, P<0.001). The expression of Smad2/3 in lesions with “stiff rim sign” was statistically higher than that in lesions without “stiff rim sign” (P<0.001). Moreover, the combination of Smad2/3 expression and “stiff rim sign” was shown to greatly raise the sensitivity (100%) and accuracy (94.56%) in the differential diagnosis of mammary gland disease.ConclusionsIn light of the findings, maximum, mean elasticity, elasticity ratio of lesions to peripheral parenchyma, and “stiff rim sign” are correlated with the expression of Smad2/3. The combination of the expression of Smad2/3 and “stiff rim sign” might contribute to the diagnosis of breast carcinoma.

Initial Acoustoelastic Measurements in Olivine: Investigating the Effect of Stress on P‐ and S‐Wave Velocities

AbstractIt is well known that elasticity is a key physical property in the determination of the structure and composition of the Earth and provides critical information for the interpretation of seismic data. This study investigates the stress‐induced variation in elastic wave velocities, known as the acoustoelastic effect, in San Carlos olivine. A recently developed experimental ultrasonic acoustic system, the Directly Integrated Acoustic System Combined with Pressure Experiments (DIASCoPE), was used with the D‐DIA multi‐anvil apparatus to transmit ultrasonic sound waves and collect the reflections. We use the DIASCoPE to obtain longitudinal (P) and shear (S) elastic wave velocities from San Carlos olivine at pressures ranging from 3.2–10.5 GPa and temperatures from 450–950°C which we compare to the stress state in the D‐DIA derived from synchrotron X‐ray diffraction. We use elastic‐plastic self‐consistent (EPSC) numerical modeling to forward model X‐ray diffraction data collected in D‐DIA experiments to obtain the macroscopic stress on our sample. We can observe the relationship between the relative elastic wave velocity change (ΔV/V) and macroscopic stress to determine the acoustoelastic constants, and interpret our observations using the linearized first‐order equation based on the model proposed by Hughes and Kelly (1953), https://doi.org/10.1103/physrev.92.1145. This work supports the presence of the acoustoelastic effect in San Carlos olivine, which can be measured as a function of pressure and temperature. This study will aid in our understanding of the acoustoelastic effect and provide a new experimental technique to measure the stress state in elastically deformed geologic materials at high pressure conditions.

Phase-Aberration Correction in Shear-Wave Elastography Imaging Using Local Speed-of-Sound Adaptive Beamforming

Shear wave elasticity imaging (SWEI) is a non-invasive imaging modality that provides tissue elasticity information by measuring the travelling speed of an induced shear-wave. It is commercially available on clinical ultrasound scanners and popularly used in the diagnosis and staging of liver disease and breast cancer. In conventional SWEI methods, a sequence of acoustic radiation force (ARF) pushes are used for inducing a shear-wave, which is tracked using high frame-rate multi-angle plane wave imaging (MA-PWI) to estimate the shear-wave speed (SWS). Conventionally, these plane waves are beamformed using a constant speed-of-sound (SoS), assuming an a-priori known and homogeneous tissue medium. However, soft tissues are inhomogeneous, with intrinsic SoS variations. In this work, we study the SoS effects and inhomogeneities on SWS estimation, using simulation and phantoms experiments with porcine muscle as an abbarator, and show how these aberrations can be corrected using local speed-of-sound adaptive beamforming. For shear-wave tracking, we compare standard beamform with spatially constant SoS values to software beamforming with locally varying SoS maps. We show that, given SoS aberrations, traditional beamforming using a constant SoS, regardless of the utilized SoS value, introduces a substantial bias in the resulting SWS estimations. Average SWS estimation disparity for the same material was observed over 4.3 times worse when a constant SoS value is used compared to that when a known SoS map is used for beamforming. Such biases are shown to be corrected by using a local SoS map in beamforming, indicating the importance of and the need for local SoS reconstruction techniques.

Shear elastic surface waves and system symmetry

The effect of the anisotropy of an elastic medium and a crystal on the properties of surface waves is considered. It leads to symmetry breaking of a semi-bounded space with respect to reflection. This fact affects the possibility of the existence and properties of surface elastic shear waves. It is shown that, while maintaining the crystallographic symmetry in crystals with the surface orientation (110), the anisotropy breaks the indicated symmetry, but retains the possibility of propagation of surface waves with their strong modification. In the case of an anisotropic half-space with a thin film coating, which allows the propagation of the Love waves in an isotropic medium, anisotropy leads to the absence of such stationary waves.

Shear wave elastography evaluation of liver, pancreas, spleen and kidneys in patients with familial mediterranean fever and amyloidosis.

Amyloid deposits in a visceral organ can contribute to tissue stiffness that could be measured with shear wave elastography (SWE). We aimed to investigate changes in organ stiffness in conjunction with laboratory parameters in patients with Familial Mediterranean Fever (FMF) and amyloidosis. This prospective study included 27 FMF patients, 11 patients with amyloidosis, and 38 healthy controls. Median shear wave elasticity values of the liver, spleen, both kidneys, and pancreas on SWE were compared among study and control groups. The mean values of CRP (C-reactive protein) and ESR (erythrocyte sedimentation rate) were compared by the t-test and the median of SAA (serum amyloid A protein) was compared with the Mann-Whitney U test between FMF groups with and without amyloidosis. Spearman's correlation analysis was performed to reveal the association between stiffness values and laboratory parameters. The median liver, spleen, kidney, and pancreas elasticity values were significantly higher in the FMF group with amyloidosis compared to control subjects. The median kidney stiffness values in the FMF group with or without amyloidosis were significantly higher compared to control subjects. Median liver stiffness values in FMF patients with amyloidosis were significantly higher than FMF patients without amyloidosis. There were statistically significant positive correlations between the CRP (p = 0.001, r = 0.56), ESR (p = 0.001, r = 0.61), and SAA (p = 0.002, r = 0.53) levels with spleen stiffness, and CRP (p = 0.006, r = 0.48) and ESR (p = 0.001,r = 0.61) levels with pancreas stiffness, and ESR (p = 0.004, r = 0.51) levels with the left kidney stiffness. SWE could be a potential tool for noninvasive follow-up of FMF patients and also amyloid deposition. Both acute inflammation and amyloidosis in the FMF patients could increase organ stiffness.

Anomalous elastic behavior of tantalum at high pressures: Experimental and theoretical studies

Elastic wave velocities of polycrystalline tantalum have been measured up to 13.6 GPa at room temperature using ultrasonic interferometry technique in a multi-anvil apparatus. The bulk and shear moduli, as well as their pressure derivatives are obtained using Eulerian 3rd order finite strain equations, yielding KS0 = 193.9(29) GPa, G0 = 69.3(10) GPa, KS0′ = 3.18(5) and G0′ = 0.88(1). The Steinberg-Guinan yield stress model is examined using the current shear modulus and its pressure derivative, where a slightly lower A0 = 0.0127 GPa−1 compared to previous studies is obtained. First-principles calculations on the elasticity of tantalum have also been performed up to 200 GPa. Anomalous softening of the elastic shear constant C44 and shear wave velocity VS is observed between 80– 180 GPa, whereas the anisotropy shows a softening-stiffening behavior with increasing pressure and reaches a minimum at around 150 GPa. Such anomalies might commonly originate from the nesting of Fermi surface for Group VB metals.

Propagation and reflection of plane waves in biological tissue based on nonlocal TPL thermoelasticity

The investigation for propagation and reflection of plane waves is carried out based on three-phase-lag (TPL) thermoelasticity with the aids of Eringen's nonlocal theory in a special medium - biological tissue. The scalar and vector potential functions of Helmhotz decomposition are adopted to obtain the propagation speeds, attenuation coefficients and reflection coefficients of plane waves analytically. There are two sets of dispersive attenuated longitudinal waves whose speeds of propagation are associated with angular frequency, and independent transverse elastic shear wave which exhibits no attenuation. It is found that the blood perfusion rate has few effects on wave propagation speed and attenuation coefficient and the phase lag of temperature gradient is negligible in present configuration. The effects of working angular frequency, nonlocal parameters, phase lag times, reflecting thermal boundaries and the type of incident waves on the propagation speeds, attenuation coefficients and reflection coefficients of harmonic waves are discussed and investigated graphically.

Simultaneous Measurements of Temperature and Viscosity for Viscous Fluids Using an Ultrasonic Waveguide.

The characterisation and monitoring of viscous fluids have many important applications. This paper reports a refined ‘dipstick’ method for ultrasonic measurement of the properties of viscous fluids. The presented method is based on the comparison of measurements of the ultrasonic properties of a waveguide that is immersed in a viscous liquid with the properties when it is immersed in a reference liquid. We can simultaneously determine the temperature and viscosity of a fluid based on the changes in the velocity and attenuation of the elastic shear waves in the waveguide. Attenuation is mainly dependent on the viscosity of the fluid that the waveguide is immersed in and the speed of the wave mainly depends on the surrounding fluid temperature. However, there is a small interdependency since the mass of the entrained viscous liquid adds to the inertia of the system and slows down the wave. The presented measurements have unprecedented precision so that the change due to the added viscous fluid mass becomes important and we propose a method to model such a ‘viscous effect’ on the wave propagation velocity. Furthermore, an algorithm to correct the velocity measurements is presented. With the proposed correction algorithm, the experimental results for kinematic viscosity and temperature show excellent agreement with measurements from a highly precise in-lab viscometer and a commercial resistance temperature detector (RTD) respectively. The measurement repeatability of the presented method is better than 2.0% in viscosity and 0.5% in temperature in the range from 8 to 300 cSt viscosity and 40 to 90 °C temperature.

Wave propagation in granular material: What is the role of particle shape?

Wave propagation in granular materials is one of the most fundamental problems in mechanics and physics, with both scientific fascination and practical importance. Whether elastic waves in granular media are affected by particle morphology and, if yes, how they are affected remain open questions. Here we present a novel grain-scale model to address these fundamental questions. Efficient techniques are incorporated in the model to cope with a huge number of non-spherical particles which are randomly packed to propagate elastic waves. The variability of particle shape is mathematically described using the superquadric function and a family of geometric shapes is produced so that a systematic investigation of the effect of particle shape becomes viable. The difficulty with the detection of particle contacts that are represented by nonlinear Hertzian contact law is tackled using an efficient algorithm. A marked finding from the multiple series of simulations using this new model is that: an increase in the aspect ratio of particles (i.e. particles changing from spherical to ellipsoidal) leads to a notable rise in the elastic wave velocity, for both compression and shear waves, whereas for non-spherical particles with a given aspect ratio, an increased particle blockiness causes a moderate reduction in the wave velocity. Moreover, it is found that an assembly of particles with higher aspect ratio is associated with a broader range of transmitted frequencies while an assembly of particles with magnified blockiness holds back the conduction of higher frequencies. Based on statistical analyses, we further show that the transition from spherical to non-spherical particles is associated with a broader range of void ratios and increased coordination numbers whereas inflated blockiness brings an opposite impact, and these changes are linked with the observed effect of particle shape on the characteristics of elastic waves, in both time and frequency domains.

Band structure analysis of Green-Naghdi-based thermoelastic wave propagation in cylindrical phononic crystals with energy dissipation using a meshless collocation method

In this paper, a meshless collocation method based on the generalized finite difference (GFD) method is employed for the band structure analysis of thermoelastic waves propagating in the cylindrical phononic crystal (PC). The Green-Naghdi (GN) theory of the generalized coupled thermoelasticity with energy dissipation and the Bloch's theorem are adopted to compute the dispersion relation of thermoelastic waves. The proposed GFD-based meshless collocation method is used to predict the frequency range of the band-gaps and to analyze the effects of the material parameters and the thickness of each layer (sub-cylinder) in a unit-cell on the band-gap property of thermoelastic waves propagating in the cylindrical PCs. The effects of the variations in the layer thicknesses in each unit-cell on the thermoelastic wave band structures are studied in detail. Also, the influences of the key material parameters on the frequency band structures of the thermoelastic waves are investigated. It will be shown that the dimensionless speed of the thermal wave influences the band-gaps more remarkably than other dimensionless parameters such as the dimensionless speeds of the purely elastic dilatational and shear waves, the damping coefficient and the thermoelastic coupling parameter.

Role of Ultrasound Elastography in Patient Selection for Prostatic Artery Embolization

PurposeTo determine the effects of prostatic artery embolization (PAE) on prostate elasticity as assessed using ultrasound elastography (US-E) and to describe baseline US-E's potential role in patient selection. Materials and MethodsThis was a prospective investigation that included 20 patients undergoing PAE to treat lower urinary tract symptoms attributed to benign prostatic hyperplasia (BPH). US-E with measurement of the prostatic elastic modulus (EM) and shear wave velocity (SWV) was performed before PAE and at 1-month follow-up. Baseline, 3-month, and 1-year follow-up evaluations included prostate-specific antigen, uroflowmetry, pelvic magnetic resonance imaging, and clinical assessment using the International Prostate Symptom Score (IPSS) and quality of life (QoL) metrics. ResultsSeventeen patients entered statistical analysis. US-E showed a significant reduction in mean prostatic EM (34.4 kPa vs 46.3 kPa, −24.7%, P < .0001) and SWV (3.55 m/s vs 4.46 m/s, −20.0%, P < .0001) after PAE. There were moderate positive correlations between baseline EM and 1-year IPSS (R = 0.62, P = .007) and between baseline SWV and 1-year IPSS (R = 0.68, P = .002). Baseline SWV ≥ 5.59 m/s and baseline EM ≥ 50.14 kPa were associated with suboptimal IPSS and QoL outcomes after PAE with high degrees of sensitivity (100%) and specificity (69-100%). ConclusionsPAE led to a positive effect on the BPH dynamic component related to prostatic elasticity. There was a moderate positive correlation between baseline prostatic elastographic parameters and 12-month IPSS. Measurement of baseline elastographic characteristics may become useful for the evaluation and selection of patients for PAE.

A Shift in Tissue Stiffness During Hippocampal Maturation Correlates to the Pattern of Neurogenesis and Composition of the Extracellular Matrix.

Aging changes the mechanical properties of brain tissue, such as stiffness. It has been proposed that the maintenance and differentiation of neural stem cells (NSCs) are regulated in accordance with extracellular stiffness. Neurogenesis is observed in restricted niches, including the dentate gyrus (DG) of the hippocampus, throughout mammalian lifetimes. However, profiles of tissue stiffness in the DG in comparison with the activity of NSCs from the neonatal to the matured brain have rarely been addressed so far. Here, we first applied ultrasound-based shear-wave elasticity imaging (SWEI) in living animals to assess shear modulus as in vivo brain stiffness. To complement the assay, atomic force microscopy (AFM) was utilized to determine the Young’s modulus in the hippocampus as region-specific stiffness in the brain slice. The results revealed that stiffness in the granule cell layer (GCL) and the hilus, including the subgranular zone (SGZ), increased during hippocampal maturation. We then quantified NSCs and immature neural cells in the DG with differentiation markers, and verified an overall decrease of NSCs and proliferative/immature neural cells along stages, showing that a specific profile is dependent on the subregion. Subsequently, we evaluated the amount of chondroitin sulfate proteoglycans (CSPGs), the major extracellular matrix (ECM) components in the premature brain by CS-56 immunoreactivity. We observed differential signal levels of CSPGs by hippocampal subregions, which became weaker during maturation. To address the contribution of the ECM in determining tissue stiffness, we manipulated the function of CSPGs by enzymatic digestion or supplementation with chondroitin sulfate, which resulted in an increase or decrease of stiffness in the DG, respectively. Our results illustrate that stiffness in the hippocampus shifts due to the composition of ECM, which may affect postnatal neurogenesis by altering the mechanical environment of the NSC niche.