Elastic Fibers Research Articles

Chinese ink-coated elastic PU/PET fibers as multifunctional flexible wearable sensors

Textiles as conductive substrates are widely used in sensing applications due to their good elasticity, high stretchability and recoverable deformation. Here, the mask rope (polyurethane/polyester blended fibers) was selected as flexible substrate to load of conductive Chinese ink using surface swelling technique in N, N-dimethylformamide. The presence of carbon nanoparticles in the ink imparts electrical conductivity to the elastic fiber while maintaining the mechanical properties and extensibility of the fiber. This simple and low-cost method allows excellent interfacial adhesion between the conductivity ink layer and the elastic fibers, and the carbon particles do not fall off even when the modified fiber was treated by sonication in water. The ink coated elastic fiber as strain sensor shows excellent stability and sensitive responsiveness after 500 stretch-release cycles. The response and recovery times during the sensitivity experiment of the flexible fiber sensor are 133.79 and 134.76 ms. The sensor exhibits physiological motion detection and quality monitoring capabilities, which can accurately monitor human movements such as walking, wrist movement, throat swallowing and articulation. The sensor also has a good Joule heating effect, and the temperature of the conductive fiber can increase 88.9°C in the 60s under 15V voltage. This work develops a simple and efficient, low-cost, mass-producible flexible sensor with potential for physiological activity, temperature, and humidity detection.

Shock wave damage from the ventral side in primary blast injury: An experimental study in pigs

Aim/PurposeThis study aimed to apply a shock wave from the ventral side of a pig and examine its effect to use the results for new body armor production for humans. MethodsSeven male hybrid pigs were used. Each pig was placed under general anesthesia on the experimental table in a blast tube to expose the left lateral position from the front chest area, and shock waves generated by compressed air at 3.0 MPa were applied. We examined changes in vital signs and arterial blood gas in the hyper-acute phase and computed tomography (CT) images, and autopsies were performed for organ damage after 3 h of observation. Pathological examination was performed for lung damage, which is considered a characteristic of shock wave injury. ResultsAll seven pigs survived. Respiratory arrest occurred in two pigs; however, spontaneous breathing resumed promptly afterward. Hypotension occurred at a frequency of 4. No bradycardia or cardiac arrest was observed in any pig. In the arterial blood gas analysis before and immediately after shock wave exposure and 1 hour later, PaO2 decreased immediately but tended to improve thereafter. CT revealed pulmonary contusions and multiple bulla-like lesions on the surface of the lungs. An autopsy showed lung injury in all pigs, particularly in five cases with bulla-like lesions of various sizes on the lung surface across all lobes. Pathological findings showed visceral pleural detachment with elastic fibers from the lung parenchyma, and the cavity lesion on the lung surface comprised bullae. The degree of intra-abdominal hemorrhage varied; however, all but one case showed splenic injury. ConclusionNone of the pigs exposed to shock waves from the ventral side died; however, most showed multiple bullae on the lung surface with lung contusion and splenic injury, which may have been greater than those exposed from the dorsal side. This may be due to the direct impact of the shock wave proceeding from the epigastrium and subcostal region, which are not protected by the skeletal structure of the thorax. These characteristics should be considered when producing new body armor for humans to protect the body from shock waves.

KMnO4/Pb staining allows uranium free imaging of tissue architectures in low vacuum scanning electron microscopy

Scanning electron microscopy under low-vacuum conditions allows high-resolution imaging of complex cell/tissue architectures in nonconductive specimens. However, the conventional methods for metal staining of biological specimens require harmful uranium compounds, which hampers the applications of electron microscopy. Here, we introduce a uranium-free KMnO4/Pb metal staining protocol that allows multiscale imaging of extensive cell/tissue architectures to intensive subcellular ultrastructures. The obtained image contrast was equivalent to that of Ur/Pb staining and sufficient for ultrastructural observation, showing the fine processes of podocytes in the glomerulus, which were invisible by light microscopy. The stainability in the elastic tissue indicated that the distinct histochemical properties of KMnO4 oxidation led to Pb deposition and BSE signal enhancement superior to Ur staining. Elemental analysis clarified that the determinant of the backscattered electron signal intensity was the amount of Pb deposition enhanced by KMnO4 oxidation. This user-friendly method is anticipated to create a new approach for biomedical electron microscopy.

Development of air therapy as a novel therapeutic branch in the field of rehabilitation.

Developing technology in the field of rehabilitation is vital to accelerate recovery and decrease the side effects of current modalities. Rehabilitation is a challenging science in which the main challenge is not just treating the patient but also to shorten the rehabilitation time and avoid harmful effects. Thus, this review demonstrates the possible design and effects of air therapy as a novel treatment branch besides hydrotherapy, electrotherapy, and manual therapy in the field of rehabilitation. The search was conducted over clinical trials, literature reviews, and systematic reviews on the possible effects of treatments that may have similar effects to the newly developed air therapy. This search was conducted in the Web of Science, Scopus, EBSCO, and Medline databases. Air therapy could be used to improve the function of mechanoreceptors, improve circulation and microcirculation, decrease pain, the release of trigger points, regain the elasticity of soft tissues, treat acute and chronic inflammations, decrease muscle cramps and spasticity, strengthen muscle, and decrease muscle fatigue and Decreasing muscle fatigue and delayed muscle soreness. Air therapy is a novel treatment modality that can be used effectively in the field of rehabilitation. Air therapy could be a valuable and safe treatment in rehabilitation.

Age- and sex-specific biomechanics and extracellular matrix remodeling of the ascending aorta in a mouse model of severe Marfan syndrome.

Thoracic aortic aneurysm (TAA) is associated with Marfan syndrome (MFS), a connective tissue disorder caused by mutations in fibrillin-1. Sexual dimorphism has been recorded for TAA outcomes in MFS, but detailed studies on the differences in TAA progression in males and females and their relationships to outcomes have not been performed. The aims of this study were to determine sex differences in the diameter dilatation, mechanical properties, and extracellular matrix (ECM) remodeling over time in a severe mouse model (Fbn1mgR/mgR = MU) of MFS-associated TAA that has a shortened life span. Male and female MU and wildtype (WT) mice were used at 1-4 mo of age. Blood pressure and in vivo diameters of the ascending thoracic aorta were recorded using a tail-cuff system and ultrasound imaging, respectively. Ex vivo mechanics and ECM remodeling of the aorta were characterized using a biaxial test system and multiphoton imaging, respectively. We showed that mechanical properties, such as structural and material stiffness, and ECM remodeling, such as elastic and collagen fiber content, correlated with diameter dilatation during TAA progression. Male MU mice had accelerated rates of diameter dilatation, stiffening, and ECM remodeling compared with female MU mice which may have contributed to their decreased life span. The correlation of mechanical properties and ECM remodeling with diameter dilatation suggests that they may be useful biomarkers for TAA progression. The differences in diameter dilatation and life spans in male and female MU mice indicate that sex is an important consideration for managing thoracic aortic aneurysm in MFS. NEW & NOTEWORTHY Using a mouse model (Fbn1mgR/mgR = MU) of severe thoracic aortic aneurysm in Marfan syndrome (MFS), we found that male MU aorta had an accelerated time line and increased amounts of dilatation, stiffening, and extracellular matrix (ECM) remodeling compared with female MU aorta that may have contributed to an increased risk of fatigue failure with cyclic loading over time and a reduced life span. We suggest that aortic stiffness may provide useful information for clinical management of aneurysms in MFS.

Amine-impregnated elastic carbon nanofiber aerogel templated by bacterial cellulose for CO2 adsorption and separation

Carbon aerogel has become a promising electrode material for CO2 capture and capacitor due to its large specific surface area, well-developed pore structure, adjustable porosity and ultra-high specific power. However, most of the carbon-based materials suffer from poor mechanical properties, low recycling efficiency, and low adsorption capacity, making it difficult to be put into industrial applications on a large scale. Bacterial cellulose (BC) has an ultra-fine reticular structure, a modulus of elasticity that is several to more than ten times that of typical plant fibers, and high tensile strength. In this paper, BC was treated by unidirectional freeze-drying method, and the aerogels with ordered honeycomb pore structure were constructed by unidirectional growth of ice crystals, and the pyrolytic chemical properties of BC were adjusted by using (NH4)2SO4, which effectively prevented the shrinkage and deformation of materials during carbonization, and successfully prepared elastic nanocarbon fiber aerogels. At the same time, the introduction of (NH4)2SO4 made the material realize N doping in the carbonization process, providing more adsorption sites, which was conducive to the adsorption of CO2. Finally, the carbon fiber nanoaerogel was modified by TEPA to further improve its adsorption selectivity for CO2. The prepared elastic carbon nanofiber aerogel (CBCNT) has high CO2 adsorption performance and high selectivity, and its CO2 capture capacity is up to 4.88 mmol/g. At the same time, its capacitance also reached 246F/g.

The first Japanese case of autosomal dominant cutis laxa with a frameshift mutation in exon 30 of the elastin gene complicated by small airway disease with 8 years of follow-up

BackgroundCutis laxa constitutes a diverse group of connective tissue diseases, both inherited and acquired, characterized by loose skin and varying systemic involvement, including pulmonary lesions. While cutis laxa has been linked to conditions like emphysema, asthma, and bronchiectasis, the specific pathological and radiological characteristics underlying pulmonary complications related to cutis laxa remain unclear.Case presentationA 36-year-old woman, diagnosed with cutis laxa at birth, presented to our outpatient clinic with severe obstructive ventilatory impairment, evident in pulmonary function tests (expiratory volume in one second (FEV1)/forced vital capacity (FVC): 34.85%; %residual volume [RV]: 186.5%; %total lung capacity [TLC]: 129.2%). Pulmonary function tests also indicated small airway disease (%FEF50%, 7.9%; %FEF75%, 5.7%; and %FEF25–75%, 6.8%). Computed tomography (CT) revealed the lack of normal increase in lung attenuation on expiratory CT scan, with no discernible emphysematous changes. Exome sequencing was performed to confirm the association between the pulmonary lesions and cutis laxa, revealing a frameshift variant in exon 30 of the elastin gene (ELN). Further analysis employing a parametric response map revealed a longitudinal increase in the percentage of functional small airway disease (fSAD) from 37.84% to 46.61% over the 8-year follow-up, despite the absence of overt changes in CT findings, specifically the lack of normal increase in lung attenuation on expiratory CT scan. Over the same follow-up interval, there was a modest reduction of 25.6 mL/year in FEV1 coupled with a significant increase in %RV. Pulmonary function test metrics, reflective of small airway disease, exhibited a continual decline; specifically, %FEF50%, %FEF75%, and %FEF25–75% diminished from 7.9% to 7.0%, 5.7% to 4.6%, and 6.8% to 5.4%, respectively.ConclusionsThis case highlighted an instance of autosomal dominant cutis laxa arising from a frameshift variant in exon 30 of ELN, accompanied by small airway disease. Comprehensive investigation, utilizing quantitative CT analysis, revealed a longitudinal increase in fSAD percentage with a mild reduction in FEV1. These findings indicate that elastin deficiency may not only diminish elastic fibers in the skin but also be implicated in small airway disease by impacting components of the extracellular matrix in the lungs.

Interplay of atherosclerosis and medial degeneration in human ascending aorta

The previous understanding has been that atherosclerosis tends to increase distally from the ascending aorta, but recent studies and practical experience have indicated that atherosclerosis occurs in the ascending aorta more than previously thought. Medial degeneration is linked to aortic aneurysms, dissection and dilatation and has been related to increased mortality. There is a lack of data on the coexistence of atherosclerosis and medial degeneration in the ascending aorta and its outcome to clinical morbidity and mortality. Earlier studies have shown coexisting atherosclerosis and medial degeneration as significant risk indicators for coronary and cerebrovascular events. We aimed to analyze aortic specimens classified according to the consensus documents of the Society for Cardiovascular Pathology and the Association for European Cardiovascular Pathology particularly the comparison of variable morphological features with the atherosclerotic grade to gain more data about the coexistence of atherosclerosis and medial degeneration. We evaluated 217 specimens of human ascending aorta resected at Tampere University Heart Hospital because of aortic aneurysm, dissection or dilatation. None of the samples contained normal aortic morphology; atherosclerosis was found in a total of 75.8% of the samples and medial degeneration in all the samples. The present study is mostly in agreement with earlier research regarding the prevalence of different histological findings, even though a higher prevalence of atherosclerosis was found compared with most studies. There was no statistically significant association between atherosclerosis and medial degeneration, but a higher atherosclerotic grade was significantly associated with the presence of smooth muscle cell nuclei loss, smooth muscle cell disorganisation, elastic fibre thinning and medial fibrosis. Our study reinforces the perception that atherosclerotic lesions significantly occur in the ascending aorta and coexist with individual components of the medial degeneration.

Histological characterization of rat vocal fold across different postnatal periods.

To evaluate the vocal fold histological characteristics during different postnatal periods in rats, especially older rats. Sprague-Dawley rats aged 4 days, 4 and 12 weeks, and 12 and 24 months were used for the experiment. Five larynges were obtained for each age and cut into 5-μm consecutive sections. The expression of Ki-67 was assessed using immunohistochemistry to examine cell proliferation. Elastic van Gieson staining was used to detect the collagen and elastin concentrations. The cell type was determined using multicolor immunofluorescence. Ki-67 was not expressed in the macula flava (MF) of 12-week-, 12-month-, and 24-month-old adults. Collagen fibers in the lamina propria (LP) increased with age. The elastic fiber concentrations in the LP decreased significantly at 24 months (p < .01) but remained stable in the MF. All posterior MF cells showed strong glial fibrillary acidic protein and vimentin-positive reactions with weaker expressions of CD68 and α-smooth muscle actin (α-SMA). The myofibroblasts (α-SMA-positive) and macrophages (CD68-positive) in the LP of the 24-month-old rats were significantly the highest (p < .01). The extracellular matrix in the LP increases with age, presenting as an increase in collagen with the loss of elastin, which may be due to myofibroblast proliferation. Moreover, the cellular properties or extracellular matrix components of the mature MF in rats are comparable to those in humans.

Color tunable photo-thermochromic elastic fiber for flexible wearable heater

Color tunable photo-thermochromic elastic fiber for flexible wearable heater

HMGB2 promotes smooth muscle cell proliferation through PPAR-γ/PGC-1α pathway-mediated glucose changes in aortic dissection

Background and aimsAortic dissection (AD) is a fatal condition with a complicated pathogenesis. High mobility group protein B2 (HMGB2) is a member of the high mobility group protein family; HMGB2 is involved in innate immunity and inflammatory diseases, but its role in AD remains unclear. MethodsHMGB2-/- mice were generated and treated with β-aminopropionitrile and angiotensin II (Ang II) to an establish AD model. An F12 gel containing AAV9-HMGB2 was applied to overexpress HMGB2 in mice. Pathological changes in the aorta were assessed by visualizing vascular collagen deposition and elastic fiber fracture via H&E, Masson and EVG staining. HMGB2 expression was measured by Western blotting and immunohistochemistry. The MTS, CCK-8 and EdU assays were used to test cell proliferation. ResultsHMGB2 expression was increased in samples from AD patients, samples from AD model mice and human aortic smooth muscle cells (HASMCs). HMGB2 promoted HASMC proliferation. Immunofluorescence staining and plasma membrane protein isolation revealed that HMGB2 decreased GLUT1 expression and promoted GLUT4 translocation. HMGB2 was also found to inhibit the expression of SIRT1/PGC-1α, but blocking the PPAR-γ pathway attenuated this effect. HMGB2-/- significantly reduced the incidence and mortality rates of AD, whereas treatment with AAV9-HMGB2 exacerbated AD. ConclusionsThis study suggested that HMGB2 promotes HASMC proliferation and vascular remodeling by regulating glucose metabolism through the PPAR-γ/SIRT1/PGC-1α pathway. HMGB2 knockdown reduced, whereas HMGB2 overexpression promoted, the occurrence of AD in mice. This study may help elucidate the underlying mechanisms and provide a new preventive target for AD.

Changes of collagen content in lung tissues of plateau yak and its mechanism of adaptation to hypoxia.

Collagen is crucial for tissue structure, functional maintenance, and cellular processes such as proliferation and differentiation. However, the specific changes in collagen expression and its associated genes in the lung tissues of yaks at high altitudes and their relationship with environmental adaptation remain poorly understood. Studying differences in the content of collagen fibers and gene expression between yaks at high (4,500 m) and low (2,600 m) altitudes, as well as between cattle at low altitudes (2,600 m). Using Masson staining, we found that the collagen fiber content in the lung tissues of yaks at low altitude was significantly higher compared to yaks at high altitude and cattle at the same altitude (P < 0.05). It was revealed through transcriptomic analyses that genes differentially expressed between high and low altitude yaks, as well as between low altitude yaks and cattle, were notably enriched in pathways related to cell adhesion, collagen synthesis, focal adhesion, and ECM-receptor interactions. Specifically, genes involved in mesenchymal collagen synthesis (e.g., COL1A1, COL1A2, COL3A1), basement membrane collagen synthesis (e.g., COL4A1, COL4A2, COL4A4, COL4A6), and peripheral collagen synthesis (e.g., COL5A1, COL6A1, COL6A2, COL6A3) were significantly upregulated in the lung tissues of yaks at low altitude compared to their high altitude counterparts and cattle (P < 0.05). In conclusion, yaks at lower altitudes exhibit increased collagen synthesis by upregulating collagen gene expression, which contributes to maintaining alveolar stability and septal flexibility. Conversely, the expression of collagen genes in yak lung tissues was down-regulated with the increase in altitude, and it was speculated that the decrease in collagen may be used to constrain the function of elastic fibers that are more abundant at high altitude, so as to enable them to adapt to the harsh environment with hypoxia and high altitude. This adaptation mechanism highlights the role of collagen in environmental acclimatization and contributes to our understanding of how altitude and species influence collagen-related physiological processes in yaks.

Pulse-Echo Ultrasound for Quantitative Measurements of Two Uncorrelated Elastic Parameters

ObjectiveThis paper describes the relationship between elastic tissue properties and strain and presents an initial investigation of pulse-echo ultrasound to measure two uncorrelated elastic parameters in tissue-mimicking phantoms. The two elastic parameters are the shear modulus, related to deformation of shape, and what we in the paper define as the nonlinear compressibility, related to deformation of volume. MethodsWe prepared tissue-mimicking phantoms containing lesions of variable shear modulus and variable nonlinear compressibility. An in-house framework for shear wave imaging was developed using ultrasound radiation force at 4.5 MHz to induce shear waves and plane wave imaging with pulses in a frequency band centered around 12.5 MHz to track the shear waves. For measurements of nonlinear compressibility, co-propagating dual-frequency pulse complexes at 0.7 MHz and 14 MHz were applied. Algorithms were implemented on a Verasonics Vantage ultrasound scanner and a custom-made multi-frequency ultrasound transducer was used. Mechanical indentation measurements were performed to validate ultrasound measurements of the shear modulus. For the nonlinear compressibility, ultrasound measurements were compared to results derived from the literature. ResultsWe found good agreement in elasticity results from ultrasound measurements and mechanical indentation as well as when comparing with results derived from the literature. ConclusionResults of the current investigation were promising. We plan patient studies involving thyroid lesions and liver steatosis to explore whether measurements of elastic parameters related both to shape deformation and volume deformation are useful in clinical practice.

The Role of Strain Wave Elastography in The Evaluation of Renal Fibrosis in Patients with Kidney Diseases

Background: A renal biopsy represents the gold standard in the diagnosis, prognosis and management of patients with chronic kidney disease and glomerulonephritis. Strain wave elastography (SE) is a developing technique to assess tissue elasticity. The aim of this study was to correlate between the strain index value of renal parenchyma and degree of renal fibrosis detected with renal biopsy.Method: For 68 patients who were referred for a kidney biopsy, SE test was performed. The Banff scoring system was utilized to classify the IFTA grading of kidney fibrosis that assigns a severity level of mild, moderate, or severe. Receiver operating characteristic curve (ROC) was utilized to correlate between the severity of renal fibrosis and the grade of renal elasticity determined by SE.Results: In total, 38 males and 30 females, the echogenicity, qualitative and semiquantitative elastography showed significant positive correlation with serum creatinine, percentage of fibrosis, G score and tubular atrophy and significant negative correlation with eGFR. ROC curve of SE for diagnosis of interstitial fibrosis shown that echogenicity has sensitivity 100.0%, Specificity 62.5%, positive predictive value (PPV) 75.0%, negative predictive value (NPV) 100.0% with area under curve (AUC) 0.906, while qualitative elastography has sensitivity 77.8%, specificity 75.0%, PPV 77.8%, NPV 75.0% AUC 0.833, semi quantitative elastography has sensitivity 83.3%, specificity 93.8%, PPV 93.8%, NPV 83.3% with AUC 0.915.Conclusion: SE approach is simple to use, and can differentiate between varying stages of renal fibrosis. However, further research is required before it can be frequently used in clinical practice.

Morphological rationale for surgical treatment of patients with true splenic artery aneurysm

Aim. To study the morphological features of the wall structure of the splenic artery aneurysm in order to identify the safest and most effective method of surgical treatment.Materials and methods. From 2020 to 2023, 43 patients underwent surgery for true splenic artery aneurysm. Interventions involved laparoscopic clipping of aneurysm branches, laparoscopic resection of aneurysm, laparoscopic splenectomy, and resection of giant splenic artery aneurysm. A clinical and morphological study of 16 slides of splenic artery aneurysm was performed. The aneurysm wall and the adjacent wall of the splenic artery without macroscopic signs of lumen dilatation for 1 cm from the aneurysmal dilatation were examined.Results. Microscopic study revealed fragments of loose, edematous wall of large elastic arteries with atherosclerosis, atheromatosis and calcification. Atherosclerotic plaques were observed in the intima; calcium deposits were detected in 81.25% of slides, and defects in elastic fibers – in all slides. The elastic membrane was thinned or had a discontinuous structure. Morphological changes in the wall of the splenic artery persisted up to 1.0 ± 0.2 cm proximal and distal to the aneurysm.Conclusion. Taking into account the revealed features of the morphological structure of the aneurysm, the formation of an end-to-end vascular anastomosis of the splenic artery, clipping or suturing of the aneurysm neck pose a significant risk of aneurysm recurrence. Laparoscopic clipping of splenic artery aneurysm branches at a distance of &gt;1.5 cm from the edges of the aneurysm becomes the operation of choice for patients in this category.

Application of Computer-Aided Design Software to the Simulation and Analysis of Composite Material Properties in Housing Engineering Structures

Reinforced polymer composites are widely used in engineering applications due to their excellent mechanical properties, and this paper combines experiments and numerical simulations to fully examine the properties of the composites. The article takes the reinforced concrete structure of civil engineering as the basis for experimental testing on the basic properties of carbon fiber composites in its application. Subsequently, the intrinsic model of reinforced concrete and carbon fiber composites was constructed, and each finite element unit of the member was selected to simulate and finite element analysis the performance of the composites through ABAQUS computer-aided design software. The results show that increasing the diameter of carbon fibers will reduce the number of carbon fibers at the same time, which will reduce the toughness of the composite material, random and chaotic distribution of carbon fibers for the improvement of the compressive properties of concrete can achieve a relatively balanced effect, and the high elastic modulus carbon fibers for the improvement of uniaxial and cyclic compressive properties of concrete play a dominant role. In addition, the oriented elliptical fibers in the long-axis direction have a significant increase in the modulus of elasticity and strength of the material, and this phenomenon will become more and more obvious with the increase of the length-to-diameter ratio. The reasonable structural design may help to improve the performance of composites and enhance their adaptability for engineering applications.

Analysis of Schlemm's Canal Measurement and Evaluation with Fourier-Domain Optical Coherence Tomography in Primary Open-Angle Glaucoma

The most common cause of permanent vision loss or blindness in the world is glaucoma. Primary Open Angle Glaucoma (POAG) develops when the eye drainage canals clog over time, increasing intraocular pressure and damaging the optic nerve. In POAG, an ophthalmology examination usually shows an open anterior chamber and chronic, irreversible multifactorial optic neuropathy followed by central visual field loss. OCT is specifically employed to investigate the elasticity of tissue, particularly in Schlemm's canal. This study aims to analyzed the morphological parameters of Schlemm's canal among patients with POAG and normal healthy control. In this observational study, we included one hundred patients, among them 50 patients had POAG, and 50 healthy controls, and both eyes were assessed. We measured the study cohort's and the healthy cohort's eyes' elevated IOP and the Schlemm's canal cross-sectional area (CSA). RT-Vue OCT was used to analyze both eyes' temporal, superior, inferior, and nasal regions. The data were analyzed using both univariate and bivariate methods. The characteristics of the morphological measurement of Schlemm's canal results were similar to those of Kagemann et al. There was a significant difference between age and SC measurement, but no correlation was observed between SC measurement and IOP or visual acuity. OCT is a useful way to evaluate the morphological status of Schlemm Canal in glaucoma patients. The eyes with POAG had smaller Schlemm's Canal- CSA than the normal eyes. The Schlemm canal-CSA in Chinese people had no differences from that of peoples of other races.

Baseline computed tomography imaging findings could assist in early diagnosis of visceral pleural invasion for newly discovered early subpleural non-small cell lung cancer: T1 or T2.

Preoperative accurate visceral pleural infiltration (VPI) diagnosis for T1-size non-small cell lung cancer (NSCLC) is significant for clinical decision-making. The study aimed to explore the diagnostic efficacy of computed tomography (CT) imaging features and serum biomarkers in diagnosing VPI in newly discovered subpleural NSCLC ≤3 cm. There were 447 patients with NSCLC ≤3 cm retrospectively enrolled and assigned to the VPI group (n=81) and the non-VPI group (n=366) based on elastic fiber staining results. The serum biomarkers and CT imaging features were obtained for each subject. Univariate and multivariate analyses were used to identify the independent predictors for VPI. Area under the receiver operating characteristic (ROC) curve (AUC) was used to evaluate the diagnostic performance of each independent predictor and combined predictors in predicting VPI, with performance compared using the DeLong test. For tumor biomarkers, the VPI group had a significantly higher percentage of cases with abnormal carcino-embryonic antigen (CEA) level, cytokeratin 19 fragment (CYFRA21-1) level, and pro-gastrin-releasing peptide (ProGRP) level than that of the non-VPI group (P<0.001, P=0.003, P=0.004). However, in multivariate analysis, only the lesion-pleura relationship patterns type Ia [odds ratio (OR) =20.689; 95% confidence interval (CI): 5.058-84.622; P<0.001], type Ib (OR =5.155; 95% CI: 1.178-22.552; P=0.03), type II (OR =7.154; 95% CI: 1.733-29.53; P=0.007) with type III as reference, solid lesion density (OR =9.954; 95% CI: 4.976-19.911; P<0.001) with part-solid density as reference were identified as the independent predictors for VPI. In predicting VPI, the combined model (AUC =0.885) significantly outperformed models based on lesion density (AUC =0.833) and lesion-pleura relationship patterns (AUC =0.655) (all P<0.001). The CT predictors for VPI in patients with subpleural NSCLC (≤3 cm) were lesion density and lesion-pleura relationship patterns (pleural attachment and indentation), but not serum tumor biomarkers.

Upper eyelid lymphatic anatomy is associated with blepharoplasty recovery

BackgroundLymphatic vessels support wound recovery and absorb excess fluid. Blepharoplasty involves excess tissue excision, and the present study investigated the relationship between the lymph vessel density of excised tissue and the postoperative course. MethodsForty eyelids from 21 blepharoptosis patients who underwent blepharoplasty were enrolled. Each resected excess tissue sample was divided into four parts by three parasagittal cuts—medial, central, and lateral—and the area percentages occupied by lymphatic vessels and elastic fibers in the cut-exposed inner tissue between skin and muscle were determined by histology. The wound healing process was assessed at 2 weeks and 1, 3, and 6 months postoperatively, by using a visual analogue scale (VAS) for estimating edema and the Vancouver Scar Scale (VSS). ResultsWith increasing age, the area percentage occupied by lymphatic vessels declined significantly (r=-0.581, p<0.001) while an increase in solar elastosis was observed. Percentages of lymphatic vessels were highest at the medial side of the eyelid (p<0.05), and here their relative distribution to the “shallow layer” close to the skin was also highest (p<0.01). Independent of age, the VSS values at 2 weeks and 1 month postoperatively were significantly lower in patients with a higher area percentage of lymphatic vessels (2 weeks: p<0.05; 1 month: p<0.01). ConclusionsIn patients undergoing blepharoplasty, upper eyelid area percentages of lymphatic vessels decreased with advancing age. Higher proportions of lymphatic vessels were associated with improved wound healing outcomes.

Acute effects of cold, heat and contrast pressure therapy on forearm muscles regeneration in combat sports athletes: a randomized clinical trial.

Due to the specific loads that occur in combat sports athletes' forearm muscles, we decided to compare the immediate effect of monotherapy with the use of compressive heat (HT), cold (CT), and alternating therapy (HCT) in terms of eliminating muscle tension, improving muscle elasticity and tissue perfusion and forearm muscle strength. This is a single-blind, randomized, experimental clinical trial. Group allocation was performed using simple 1:1 sequence randomization using the website randomizer.org. The study involved 40 40 combat sports athletes divided into four groups and four therapeutic sessions lasting 20min. (1) Heat compression therapy session (HT, n = 10) (2) (CT, n = 10), (3) alternating (HCT, n = 10), and sham, control (ShT, n = 10). All participants had measurements of tissue perfusion (PU, [non-reference units]), muscle tension (T-[Hz]), elasticity (E-[arb- relative arbitrary unit]), and maximum isometric force (Fmax [kgf]) of the dominant hand at rest (Rest) after the muscle fatigue protocol (PostFat.5min), after therapy (PostTh.5min) and 24h after therapy (PostTh.24h). A two-way ANOVA with repeated measures: Group (ColdT, HeatT, ContrstT, ControlT) × Time (Rest, PostFat.5min, PostTh.5min, Post.24h) was used to examine the changes in examined variables. Post-hoc tests with Bonferroni correction and ± 95% confidence intervals (CI) for absolute differences (△) were used to analyze the pairwise comparisons when a significant main effect or interaction was found. The ANOVA for PU, T, E, and Fmax revealed statistically significant interactions of Group by Time factors (p < 0.0001), as well as main effects for the Group factors (p < 0.0001; except for Fmax). In the PostTh.5min. Period, significantly (p < 0.001) higher PU values were recorded in the HT (19.45 ± 0.91) and HCT (18.71 ± 0.67) groups compared to the ShT (9.79 ± 0.35) group (△ = 9.66 [8.75; 10.57 CI] > MDC(0.73), and △ = 8.92 [8.01; 9.83 CI] > MDC(0.73), respectively). Also, significantly (p < 0.001) lower values were recorded in the CT (3.69 ± 0.93) compared to the ShT (9.79 ± 0.35) group △ = 6.1 [5.19; 7.01 CI] > MDC(0.73). For muscle tone in the PostTh.5m period significantly (p < 0.001) higher values were observed in the CT (20.08 ± 0.19Hz) group compared to the HT (18.61 ± 0.21Hz), HCT (18.95 ± 0.41Hz) and ShT (19.28 ± 0.33Hz) groups (respectively: △ = 1.47 [1.11; 1.83 CI] > MDC(0.845); △ = 1.13 [0.77; 1.49 CI] > MDC(0.845), and △ = 0.8 [0.44; 1.16 CI], < MDC(0.845)). The highest elasticity value in the PostTh.5m period were observed in the CT (1.14 ± 0.07) group, and it was significantly higher than the values observed in the HT (0.97 ± 0.03, △ = 0.18 [0.11; 0.24 CI] > MDC(0.094), p < 0.001), HCT (0.90 ± 0.04, △ = 0.24 [0.17; 0.31 CI] > MDC(0.094), p < 0.001) and ShT (1.05 ± 0.07, △ = 0.094 [0.03; 0.16 CI] = MDC(0.094), p = 0.003) groups. For Fmax, there were no statistically significant differences between groups at any level of measurement. The results of the influence of the forearm of all three therapy forms on the muscles' biomechanical parameters confirmed their effectiveness. However, the effect size of alternating contrast therapy cannot be confirmed, especially in the PostTh24h period. Statistically significant changes were observed in favor of this therapy in PU and E measurements immediately after therapy (PostTh.5min). Further research on contrast therapy is necessary.