Elastase Inhibitory Activity Research Articles

Skin whitening and anti-corrugation activities of glycoprotein fractions from liquid extracts of boiled sea cucumber.

To determine skin whitening and wrinkle improvement efficacy, glycoprotein fractions were extracted from liquid extracts of boiled sea cucumber and their effects on tyrosine and elastase inhibitory activities were assayed. Fractions above and below 50kDa (>50kDa and <50kDa) were extracted via a series of steps involving: boiling, filtering, desalting and freeze drying. Cytotoxicity, skin whitening and wrinkle-removing effects of boiled liquid were determined. Our MTT data showed that neither glycoprotein fraction of boiled liquid induces cellular cytotoxicity up to a concentration of 10mg/mL treatment of the mouse melanoma cell line, B16F10, with 10mg/mL >50kDa enhanced tyrosinase and elastase inhibitory activities by 50.84% and 28.78%, respectively. Correlations of the >50kDa concentration with tyrosinase inhibitory (R2=0.968) and elastase inhibitory (R2=0.983) efficacy were significant. >50kDa glycoprotein fraction isolated from liquid extracts of boiled sea cucumber, which can serve as a functional cosmetic ingredient for whitening and wrinkle improvement of skin.

Recombinant Human Alpha-1 Antitrypsin-Fc Fusion Protein Reduces Mouse Myocardial Inflammatory Injury After Ischemia-Reperfusion Independent of Elastase Inhibition.

Alpha-1-antitrypsin (AAT) is an abundant plasma protein with neutrophil elastase-inhibiting activity, and AAT is available as a plasma-derived therapeutic (pAAT). In experimental myocardial infarction, pAAT reduced acute inflammatory injury because of ischemia-reperfusion. The aim of the present study was to assess the properties of a recombinant protein composed of human AAT fused to the human immunoglobulin (Ig) G1 Fc fragment (rhAAT-Fc) in experimental myocardial infarction. Ten-week-old CD1 male mice underwent transient occlusion (30 minutes) of the left anterior coronary artery. rhAAT-Fc (2 mg/kg) or pAAT (60 mg/kg) were administered upon reperfusion. We used human plasma-derived Ig (2 mg/kg) or a matching volume of NaCl 0.9% as control solutions. After 24 hours, infarct size and caspase-1 activity were quantified. The left ventricular ejection fraction (LVEF) was measured by echocardiography at 24 hours and 7 days. A variant of rhAAT-Fc lacking elastase inhibition activity, rhAAT-Fc, was also tested. The rhAAT-Fc induced a significant reduction in infarct size (P < 0.01 vs. all controls, P > 0.05 vs. pAAT). Caspase-1 activity was reduced to the same degree with rhAAT-Fc and pAAT (-70%; P < 0.05; P > 0.05 rhAAT-Fc vs. pAAT). The effects on infarct size after a single administration were reflected by preservation of LVEF at 24 hours and 7 days (all P < 0.05). rhAAT-Fc without elastase inhibiting activity, rhAAT-Fc, conferred comparable effects on infarct size, caspase-1 activity, and LVEF (P > 0.2 vs. rhAAT-Fc). The pAAT and recombinant human AAT-Fc reduce the acute myocardial inflammatory injury after ischemia-reperfusion in the mouse leading to preservation of viable myocardium and systolic function, independent on the effects on neutrophil elastase.

In Vitro Dermo-Cosmetic Evaluation of Bark Extracts from Common Temperate Trees.

Wood residues produced from forestry activities represent an interesting source of biologically active, high value-added secondary metabolites. In this study, 30 extracts from 10 barks of deciduous and coniferous tree species were investigated for their potential dermo-cosmetic use. The extracts were obtained from Fagus sylvatica, Quercus robur, Alnus glutinosa, Prunus avium, Acer pseudoplatanus, Fraxinus excelsior, Populus robusta, Larix decidua, Picea abies, and Populus tremula after three successive solid/liquid extractions of the barks with n-heptane, methanol, and methanol/water. All extracts were evaluated for their radical scavenging capacity, for their elastase, collagenase, and tyrosinase inhibitory activities, as well as for their antibacterial activity against gram-positive Staphylococcus aureus. In parallel, the global metabolite profiles of all extracts were established by 1D and 2D NMR and related to their biological activity. The results showed that the methanol extracts of Q. robur, A.glutinosa, L.decidua, and P. abies barks exhibit particularly high activities on most bioassays, suggesting their promising use as active ingredients in the dermo-cosmetic industry.

Monascus-mediated fermentation improves the nutricosmetic potentials of soybeans.

The potential nutricosmetic activities and compositional changes of 80% ethanol extracts of white soybean (MFWS) and black soybean (MFBS) fermented with Monascus pilosus KCCM 60084 at 30°C for 30 days were investigated. Monascus-fermented soybean extracts (MFSEs) showed stronger nutricosmetic potentials in terms of antioxidant as well as tyrosinase and elastase inhibitory activities compared to those of unfermented soybean extracts (p<0.05). Extracts (50mg/mL) from MFBS inhibited the α-melanocyte stimulating hormone (α-MSH)-induced melanogenesis in B16F10 cells more potently than arbutin. HPLC/MS analysis demonstrated that aglycone isoflavones and coenzyme Q10 (CoQ10) levels increased about 33.4- and 3.0-fold in the MFSEs after 20 days of fermentation, respectively. A linear correlation (r 2=0.67 to 0.99) between nutricosmetic activity and concentrations of CoQ10, genistein, and daidzein, which are commonly associated with nutraceutical effects, was observed. Results indicate that Monascus-mediated fermentation can be an efficient strategy to improve nutricosmetic functionality of soybeans.

번행초(Tetragonia tetragonioides)로부터 추출한 Phenolic Compounds의 미용 식품 활성

본 연구는 물과 ethanol을 추출용매로 사용한 번행초(Tetragonia tetragonioides) 추출물이 미용 식품 소재로 사용 가능한 기능성을 증명하고자 하였다. Phenolic compounds는 물 추출물에서 3.29 mg/g, 50% ethanol 추출물에서 4.14 mg/g의 결과를 각각 나타내었다. 전자공여능 측정 결과 200 μg/mL phenolic compounds의 농도에서 물 추출물은 98.45%, 50% ethanol 추출물은 91.20%의 활성을 나타내었다. ABTS radical 활성 측정 결과 100 μg/mL phenolic compounds의 농도에서 물 추출물은 97.28%, 50% ethanol 추출물은 97.03%의 활성을 나타내었다. Antioxidant protection factor(PF) 측정 결과 200 μg/mL phenolic compounds의 농도에서 물과 ethanol 추출물 모두 1.77 PF의 같은 결과를 나타내었다. Thiobarbituric acid reactive substance(TBARS) 측정 결과 100 μg/mL phenolic compounds의 농도에서 물과 ethanol 추출물은 94.77%와 95.64%의 높은 활성을 나타내었다. 미백과 관련 있는 tyrosinase 저해 활성을 측정한 결과 200 μg/mL phenolic compounds의 농도에서 ethanol 추출물은 34.96%의 억제력을 나타내었다. 주름 개선 효과를 측정하는 elastase 저해 활성과 collagenase 저해 활성을 측정한 결과 200 μg/mL phenolic compounds의 농도에서 ethanol 추출물만 78.90%와 61.3%의 높은 저해 활성을 나타내었다. 수렴 효과를 나타내는 astringent effect에서 200 μg/mL phenolic compounds 농도의 ethanol 추출물은 7.82%의 효과를 나타내었다. Hyaluronidase 저해 효과 측정 결과 물 추출물 200 μg/mL phenolic compounds의 농도에서 81.04%의 염증 억제 효과를 나타내었다. 이러한 결과들로 미루어 보았을 때 번행초 추출물은 항산화 작용과 미용 식품으로서의 기능성을 기대할 수 있다.

Anti-oxidation and Anti-aging Activity of Three Compositae Species at Different Extraction Temperatures

This study was conducted to determine the antioxidant and anti-aging activities of water-soluble compounds extracted from Inula Flos (IF), Chrysanthemi Flos (CSF) and Carthami Flos (CTF) at 25°C and 80°C. The 80°C CTF extract included soluble protein (386.44 mg/g), reducing sugar (101.27 mg/g), flavonoids (42.70 mg/g), and polyphenols (123.96 mg/g). The DPPH radical scavenging activities of all extracts were over 50% at 0.1 mg/mL; the 25°C CTF extract showed a significantly higher effect than ascorbic acid at 1.0 mg/mL. Xanthine oxidase inhibition, SOD-like activity and tyrosinase inhibition of the 80°C IF extract were 32.14%, 31.54%, and 42.01% at 2.0 mg/mL, respectively. The elastase and collagenase inhibition activities of the IF 80°C extract were 34.67% and 31.40%, respectively. The nitrite scavenging abilities of 25°C IF and CTF extracts along with 80°C CSF and CTF extracts were over 95% (at 2 mL, pH 1.2), even higher than ascorbic acid; and nitrite scavenging ability of the 80°C CTF extract was 87.30% (at 2 mL, pH 3.0), similar to ascorbic acid (87.36%). These results suggest that the 80°C CTF extract contained useful compounds and IF is a potentially useful antioxidant and anti-aging source for the development of functional cosmetics.

Antioxidant and protease-inhibitory potential of extracts from grains of oat

AbstractThe most of important crops cultivated for production of foods and feeds could be considered as plants possessing nutraceutical or medically interesting compounds, especially if can be eaten without processing. Chemical and biological parameters that were evaluated in 100 oat (Avena sativa L.) genotypes were others than those that are important in food and feed production. Contents of polyphenols and flavonoids, radical scavenging activity (DPPH), and inhibitory activities against five proteases (trypsin, thrombin, urokinase, elastase, cathepsin B) were analyzed in extracts from mature grains. The antioxidant activity (DPPH) correlated to the content of total polyphenols. Only a minority (15 from 100) of analyzed genotypes created separate subgroup with a high content of polyphenols, flavonoids, and high antioxidant activity. The best in these parameters were genotypes CDC-SOL-FI, Saul, and Avesta, respectively. Fifteen other genotypes assembled another minority subgroup (also 15 from 100) on the basis of their high inhibitory activities against tested proteases. The highest trypsin-, urokinase-, and elastase-inhibitory activities were in genotype Racoon, the best in thrombin-, and cathepsin B-inhibitory activities were genotypes Expression and SW Kerstin, respectively. Three oats genotypes – Rhea, AC Percy, and Detvan appeared in both subgroups.

Total Synthesis of the Potent Marine-Derived Elastase Inhibitor Lyngbyastatin 7 and in Vitro Biological Evaluation in Model Systems for Pulmonary Diseases.

Lyngbyastatin 7 (1) is a marine cyanobacteria-derived lariat-type cyclic depsipeptide of which the macrocyclic core possesses modified amino acids, including a featured 3-amino-6-hydroxy-2-piperidone (Ahp) moiety and a (Z)-2-amino-2-butenoic acid (Abu) moiety. The first total synthesis of 1 was successfully established via 31 steps, and the conditions of several crucial steps were optimized to ensure smooth operations. The previously reported structural assignment and elastase inhibitory activity of the isolated natural product were confirmed. According to the extensive in vitro biological evaluation, compound 1 displayed low nanomolar IC50 in blocking elastase activity and strong ability in protecting bronchial epithelial cells against elastase-induced antiproliferation and abrogating the elastase-triggered induction of pro-inflammatory cytokine expression. Its overall performance was superior over sivelestat, the only approved small molecule drug targeting elastase, which indicated its potential in developing as a pharmacotherapeutic against elastase-mediated pathologies. The success in total synthesis, designed with a novel convergent strategy, not only overcame the supply issue for thorough preclinical studies but also paved the way for convenient synthesis of analogues with improved potency and druglike properties.

SerpinB1 Promotes Pancreatic β Cell Proliferation

Although compensatory islet hyperplasia in response to insulin resistance is a recognized feature in diabetes, the factor(s) that promote β cell proliferation have been elusive. We previously reported that the liver is a source for such factors in the liver insulin receptor knockout (LIRKO) mouse, an insulin resistance model that manifests islet hyperplasia. Using proteomics we show that serpinB1, a protease inhibitor, which is abundant in the hepatocyte secretome and sera derived from LIRKO mice, is the liver-derived secretory protein that regulates β cell proliferation in humans, mice, and zebrafish. Small-molecule compounds, that partially mimic serpinB1 effects of inhibiting elastase activity, enhanced proliferation of β cells, and mice lacking serpinB1 exhibit attenuated β cell compensation in response to insulin resistance. Finally, SerpinB1 treatment of islets modulated proteins in growth/survival pathways. Together, these data implicate serpinB1 as an endogenous protein that can potentially be harnessed to enhance functional β cell mass in patients with diabetes.

Reference of Circadian Rhythm in the Treatment of Psoriasis : A Novel Insight

The objective of the present study was to establish t he needs for different products in the treatment of psoriasis with reference to circadian rhythm. The events like proliferation of keratinocytes and elastase activity are influenced by circadian rhythm. The proliferation of the keratinocytes occurs more during early hours of the day however, the enzymatic activities occur post to that. Psorolin ointment and Psorolin oil were studied for the cell proliferation inhibition of the keratinocytes and elastase and LDH inhibition separately. In vitro assay on growth inhibition of keratinocytes by MTT, Cytotoxicity assay by measuring LDH, Tryphan blue exclusion assay, Elastase inhibition assay, Hyaluronidase inhibition assay. Findings of the study show that Psorolin oil inhibited the proliferation of keratinocytes in a non-cytotoxic manner as proved by lactate dehydrogenase (LDH) assay and Tryphan blue staining. The Psorolin ointment effectively inhibited elastase activity. Considering the time specificity cell division and enzymatic activity are separate, warrant the need of both Psorolin oil and Psorolin ointment for the treatment of psoriasis. In the present study confirm the need of two separate drugs viz., Psorolin oil and Psorolin ointment for the treatment of psoriasis not due to their mechanism action was different but due to the effect of circadian rhythm in the pathogenesis of psoriasis. Keywords: Circadian rhythm, Psoriasis, Cytotoxicity assay, Dr. JRK’s, Psorolin Cite this Article Amruthavalli GV, Gayathri Rajagopal . Reference of Circadian Rhythm in the Treatment of Psoriasis – A Novel Insight, Research and Reviews: A Journal of Unani, Siddha and Homeopathy (RRJoUSH) . 2015; 2(2): 1–7p.

Inhibitory Effect of A grimonia Pilosa Leaf Extract on the UV-Induced Photoaging-Related Ion Channel, ORAI1, and the Enzymes Tyrosinase and Elastase

Agrimonia pilosa Ledeb. has received considerable attention as a natural product for its applications in cosmetics owing to its anti-skin wrinkling and whitening effects. The ORAI1 ion channel is known to be involved in UV-induced photoaging. Additionally, tyrosinase and elastase are key enzymes in the process of wrinkle formation and pigmentation. We determined that A. pilosa leaf extract and its fractions (dichloromethane, ethyl acetate and n-butanol) inhibit this channel and associated key enzymes. Whole-cell patch-clamp experiments demonstrated that the ethyl acetate fraction had a potent inhibitory effect on IORAI1 (99 ± 1%) at 100 μg/mL. Ethyl acetate and dichloromethane fractions had tyrosinase inhibitory activity (46.1 ± 0.84% and 35.7 ± 1.40% at 330 μg/mL, respectively). Additionally, these fractions inhibited elastase activity by 48.3 ± 1.20% and 45.4 ± 1.51% at 330 μg/mL, respectively. Our results suggest that A. pilosa leaves are a potential anti-wrinkle and whitening agent. Practical Applications Agrimonia pilosa has been widely used for the treatment of sore throat, headache, bloody dysentery, parasitic infection and eczema in traditional oriental medicine. Recently, it has also been used as an external application for the treatment of various dermatological problems, such as healing wounds, diminishing wrinkles, pigmentation and atopic dermatitis. However, to date, no report has detailed the mechanisms of A. pilosa's anti-photoaging activity. This research revealed that the extract of A. pilosa leaves meditates ORAI1 channel activity and directly inhibits tyrosinase and elastase activity. Therefore, A. pilosa may be a potential therapeutic agent in the prevention of UV-induced photoaging.

Perioperative elafin for ischaemia-reperfusion injury during coronary artery bypass graft surgery: a randomised-controlled trial

BackgroundElafin is a potent endogenous neutrophil elastase inhibitor that protects against myocardial inflammation and injury in preclinical models of ischaemic-reperfusion injury. We investigated whether elafin could inhibit myocardial ischaemia-reperfusion injury...

Anti-wrinkle Activities verification of Buplerum falcatum Extracts on CCD-986sk

The electron donating ability, elastase inhibitory, pro- collagen synthesis and Matrix metalloprotease-1 (MMP-1) activities were measured in order to verify the anti-wrinkle properties of extracts from Buplerum falcatum as a functional ingredient for cosmetic products. Electron donating ability and elastase inhibition activities were 80 and 52% at a dose of 1,000 µg/mL of B. falcatum 70% ethanol extract. Pro-collagen synthesis was increased with the increase concentration of B. falcatum extract on CCD-986sk in addition to decrease the amount of protein of MMP-1. The results suggested that B. falcatum extract can be used to reduced electron donating ability, elastase, pro-collagen synthesis and MMP-1 activity and is a potential candidate for cosmedical materials.

Total synthesis and biological evaluation of Lyngbyastatin 7 for the treatment of pulmonary diseases

Lyngbyastatin 7 is marine-derived cyanobacterial cyclodepsipeptide that showed better potency towards inhibiting elastase than sivelestat, the only approved drug targeting this enzyme. The discovery of elastase inhibitors is of great importance since this enzyme plays a significant role in several pulmonary diseases, such as chronic obstructive pulmonary disease, the third leading cause of death worldwide. We established the first total synthesis of lyngbyastatin 7 and validated the elastase-inhibitory activity in enzyme assays. Lyngbyastatin 7 also outperformed sivelestat in our cellular assays, where the compound protected human bronchial epithelial cells against elastase-induced antiproliferative effects as well as attenuated the transcription of sICAM-1 and IL1β which are induced by elastase. The synthetic accessibility overcomes the supply issue and paves the way for the synthesis of diverse analogues with the goal of discovering effective therapeutics against elastase-mediated pathologies.

Cosmeceutical potentials and bioactive compounds of rice bran fermented with single and mix culture of Aspergillus oryzae and Rhizopus oryzae

In the present study, rice bran, one of the most abundant agricultural by-products in Malaysia, was fermented with single and mixed cultures of Aspergillus oryzae and Rhizopus oryzae. The fermented rice bran extracts were tested for their functional properties and compared to the non-fermented counterparts. Antioxidant activities as well as phenolics and organic acid contents were evaluated. Skincare-related functionalities were also tested by evaluating tyrosinase and elastase inhibition activities. Tyrosinase inhibition activity, measured to determine the anti-pigmentation effect of extracts, was found to be the highest in the extract of rice bran fermented with A. oryzae (56.18%) compared to other extracts. In determining the anti-aging effect of fermented rice bran extracts, the same extract showed the highest elastase inhibition activity with a value of 60.52%. Antioxidant activities were found to be highest in the mix-cultured rice bran extract. The results of phenolic and organic acid content were varied; the major phenolic acid detected was ferulic acid with a value of 43.19μg/ml in the mix-cultured rice bran extract. On the other hand, citric acid was the major organic acid detected, with the highest content found in the same extract (214.6mg/g). The results of this study suggest that the fermented rice bran extracts may have the potential to be further exploited as ingredients in cosmetics as well as in antioxidant-rich products.

Allium 속 식물 물 추출물의 항산화 활성과 Tyrosinase 및 Elastase 저해 효과

Three different Allium plants, including wild garlic (Allium monanthum Max; AMM), onion (Allium cepa L.; ACL) and green onion (Allium fistulosum L; AFL), have been widely used as spicy food in daily life. This study was attempted to investigate the properties of antioxidant, whitening and anti-wrinkle activities in water extracts in Allium plants. The antioxidant, whitening and anti-Wrinkle activities were evaluated by performing DPPH and ABTS radical scavenging activities, inhibitory effects on tyrosinase, and elastase, respectively. The water extract (10 mg/mL) of onion, wild garlic and green onion exhibited DPPH radical scavenging activities of 28.46%, 28.45%, and 15.91%, respectively. The inhibitory effects of tyrosinase activities by wild garlic, green onion, and onion were increased by heating the extracts at . Additionally, the wild garlic extract showed higher elastase inhibitory activity than those of other plant extracts. These results suggest that water extracts of Allium plants may be useful as potential sources of beauty foods due to higher antioxidant, anti-melanin and anti-wrinkle activities.

Improved Therapeutic Profiles of PLA2-Free Bee Venom Prepared by Ultrafiltration Method.

Bee venom (BV) has long been used in traditional Eastern and Western medicine for chronic inflammation, pain and skin therapy. Human exposure to BV, however, often causes unwanted adverse effects and is even fatal in some cases. Phospholipase A2 (PLA2) of BV is now suspected to play a key role in these adverse effects. We investigated the potential use of PLA2-free bee venom (PBV) as a replacement for BV in cosmetic products. PBV prepared by molecular weight cut-off ultrafiltration exhibits a superior profile in comparison with regular BV, by inhibiting elastase activity and suppressing the induction of nitric oxide (NO) and metalloproteinase-9 (MMP-9), while retaining the effects of cell proliferation and protection against ultraviolet B (UVB)-induced damage in human dermal fibroblast cells. PBV thus appears to be more promising than BV as a cosmetic ingredient with a reduced potential for adverse reactions in the recipient.

희첨 추출물이 B16F10 세포에서 Melanin 생성과 Tyrosinase 활성에 미치는 억제효과

Objective : Siegesbeckia Herba can treat various skin disease by expelling wind and removing dampness and clearing away heat and toxic material effects. This study was designed to investigate effects of Siegesbeckia Herba Extracts(SHE) on skin elasticity and whitening using B16F10 cell lines. Method : In this experiment, We observed effect of SHE on cell viability, inhibition of melanin synthesis and inhibitory effect on tyrosinase and elastase. Results : 1. SHE treated group showed decreased cell viability rates significantly compared with non-treated group. More than SHE <TEX>$250{\mu}g/ml$</TEX>, <TEX>$500{\mu}g/ml$</TEX> and <TEX>$1,000{\mu}g/ml$</TEX> of treated groups were lower levels of melanin synthesis respectively. 2. SHE significantly showed tyrosinase inhibitory activity in vitro, SHE increased tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, and tyrosinase inhibitory activity in vitro. 3. Tyrosinase inhibitory activity and elastase inhibitory activity in B16F10 cells, tyrosinase inhibitory activity in vitro were not accepted statistical significance compared with non-treated group. 4. SHE treated group showed increased SOD-like activity rates significantly compared with non-treated group. More than SHE <TEX>$250{\mu}g/ml$</TEX>, <TEX>$500{\mu}g/ml$</TEX> and <TEX>$1,000{\mu}g/ml$</TEX> of treated groups were lower levels of melanin synthesis respectively. Conclusion : These results suggest that SHE can inhibit melanin synthesis and tyrosinase inhibtory activity. So, We suggest that SHE can be maintained skin whitening.

Purification and partial characterization of α1-proteinase inhibitor in the common marmoset (Callithrix jacchus)

Fecal alpha1-proteinase inhibitor (α1-PI) concentration has been to diagnose enteric protein loss in dogs and cats. Chronic lymphocytic enteritis is commonly seen in the marmoset (Callithrix jaccus) and is characterized by hypoalbuminemia. As a prelude to immunoassay development for detecting enteric protein loss, marmoset serum α1-PI was purified using immunoaffinity chromatography and ceramic hydroxyapatite chromatography. Partial characterization was performed by reducing gel electrophoresis and enzyme inhibitory assays. Protein identity was confirmed with peptide mass fingerprinting and N-terminal amino acid sequencing. Molecular mass, relative molecular mass, and isoelectric point for marmoset α1-PI were 54 kDa, 51,677, and 4.8–5.4, respectively. Trypsin, chymotrypsin, and elastase inhibitory activity were observed. N-terminal amino acid sequence for marmoset α1-PI was EDPQGDAAQKMDTSHH. In conclusion, marmoset α1-PI was successfully purified from serum with an overall yield of 12% using a rapid and efficient method. Purified marmoset α1-PI has characteristics similar to those of α1-PI reported for other species.

Anti-oxidation and Anti-wrinkling Effects of Jeju Horse Leg Bone Hydrolysates.

This study focused on the anti-oxidative and collagenase- and elastase inhibition effects of low molecular weight peptides (LMP) from commercial Jeju horse leg bone hydrolysates (JHLB) on pancreatin, via enzymatic hydrolysis. Cell viability of dermal fibroblasts exposed to UVB radiation upon treatment with LMP from JHLB was evaluated. Determination of the antioxidant activity of various concentrations of LMP from JHLB were carried out by assessing 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2-azino-bis-3-ethybenzothiazoline-6-sulphonic acid (ABTS) radical scavenging activity, ferric reducing antioxidant power (FRAP), and oxygen radical absorbance capacity (ORAC). The DPPH radical scavenging activity of LMP from JHLB (20 mg/mL) was 92.21% and ABTS radical scavenging activity (15 mg/mL) was 99.50%. FRAP activity (30 mg/mL) was 364.72 μM/TE and ORAC activity (1 mg/mL) was 101.85 μM/TE. The anti-wrinkle potential was assessed by evaluating the elastase- and collagenase inhibition potential of these LMP. We found that 200 mg/mL of LMP from JHLB inhibited elastase activity by 41.32%, and 100 mg/mL of LMP from JHLB inhibited collagenase activity by 91.32%. The cell viability of untreated HS68 human dermal fibroblasts was 45% when exposed to a UVB radiation dose of 100 mJ/cm2. After 24 h of incubation with 500 μg/mL LMP from JHLB, the cell viability increased to 60%. These results indicate that LMP from JHLB has potential utility as an anti-oxidant and anti-wrinkle agent in the food and cosmetic industry. Additional in vivo tests should be carried out to further characterize these potential benefits.