Anti-oxidation and Anti-aging Activity of Three Compositae Species at Different Extraction Temperatures

Abstract

This study was conducted to determine the antioxidant and anti-aging activities of water-soluble compounds extracted from Inula Flos (IF), Chrysanthemi Flos (CSF) and Carthami Flos (CTF) at 25°C and 80°C. The 80°C CTF extract included soluble protein (386.44 mg/g), reducing sugar (101.27 mg/g), flavonoids (42.70 mg/g), and polyphenols (123.96 mg/g). The DPPH radical scavenging activities of all extracts were over 50% at 0.1 mg/mL; the 25°C CTF extract showed a significantly higher effect than ascorbic acid at 1.0 mg/mL. Xanthine oxidase inhibition, SOD-like activity and tyrosinase inhibition of the 80°C IF extract were 32.14%, 31.54%, and 42.01% at 2.0 mg/mL, respectively. The elastase and collagenase inhibition activities of the IF 80°C extract were 34.67% and 31.40%, respectively. The nitrite scavenging abilities of 25°C IF and CTF extracts along with 80°C CSF and CTF extracts were over 95% (at 2 mL, pH 1.2), even higher than ascorbic acid; and nitrite scavenging ability of the 80°C CTF extract was 87.30% (at 2 mL, pH 3.0), similar to ascorbic acid (87.36%). These results suggest that the 80°C CTF extract contained useful compounds and IF is a potentially useful antioxidant and anti-aging source for the development of functional cosmetics.