This paper describes the application of remote sensing in monitoring the fluctuations in one of the mountain glaciers in the Ecuadorean Andes during the past few decades using ASTER, EO-1 ALI, Landsat MSS, TM and ETM + images. Satellite images were used to calculate the snow line altitudes (SLAs) during the period 1979–2013. Cotopaxi ice covered volcano was studied as representative of Ecuadorian glaciers in the eastern cordillera. Precipitation and air temperature data from various gauging stations within the range of 30 km from the study site and monthly discharge and water level data from a gauging station were also utilized in this study. Anomalies in precipitation and temperature were found to be slightly different in the Cotopaxi region compared to nearby Antizana in the same cordillera and Chimborazo region in the western cordillera. An attempt to correlate the El Nino—southern oscillation phenomenon with the glacier fluctuations in Ecuadorian Andes was done successfully. Cold and warm regimes of Pacific Decadal Oscillation is also considered. The calculated glacier fluctuations obtained were similar to that performed on the nearby Antizana 15 in the eastern cordillera during 1995–2002. Precipitation and temperature anomalies were similar with Antizana 15. It is evident from the research that SLAs were highly fluctuated between the period of occurrence of El Nino and La Nina events. It is also seen that the glacier fluctuations show a negative mass balance trend in during the warm regime of Pacific Decadal Oscillation during the past three decades. Glaciated areas were advanced during the La Nina events in the cold regime of PDO during 1998–2002.