Summary We report 87Sr/86Sr and ion concentrations of sulfate, chloride, and strontium in the groundwater of the northern and central Yucatan Peninsula, Mexico. Correlation between these data indicates that ejecta from the 65.95 m.y. old Chicxulub impact crater have an important effect on hydrogeology, geomorphology, and soil development of the region. Ejecta are present at relatively shallow subsurface depths in north-central Yucatan and at the surface along the Rio Hondo escarpment in southeast Quintana Roo, where they are referred to as the Albion Formation. Anhydrite/gypsum (and by inference celestite) are common in impact ejecta clasts and in beds and cements of overlying Paleocene and Lower Eocene rocks cored around the margin of the crater. The sulfate-rich minerals that are found in rocks immediately overlying the impact ejecta blanket, may either be partially mobilized from the ejecta layer itself or may have been deposited after the K/T impact event in an extensive pre-Oligocene shallow sea. These deposits form a distinctive sedimentary package that can be easily traced by the Eocene–Cretaceous 87Sr/86Sr signal. A distinct Sr isotopic signature and high SO4/Cl ratios are observed in groundwater of northwestern and north-central Yucatan that interacts with these rocks. Moreover, the distribution of the gypsum-rich stratigraphic unit provides a solution-enhanced subsurface drainage pathway for a broad region characterized by dissolution features (poljes) extending from Chetumal, Quintana Roo to Campeche, Campeche. The presence of gypsum quarries in the area is also consistent with a sulfate-rich stratigraphic “package” that includes ejecta. The distinctive chemistry of groundwater that has been in contact with evaporite/ejecta can be used to trace flow directions and confirms a groundwater divide in the northern Peninsula. Information about groundwater flow directions and about deep subsurface zones of high permeability is useful for groundwater and liquid waste management in the area. Where it discharges at the coast, the unique chemistry of the groundwater that has interacted with the evaporite/ejecta strata may also have significant geomorphologic implications. While groundwater–seawater mixing at the coast has been shown to dissolve and erode limestone, PHREEQC modeling shows that mixing of water nearly saturated in CaSO4 with seawater has a less vigorous dissolution effect due to its high Ca content.