Ehrlich Ascites Tumor-bearing Mice Research Articles

Effect of Ehrlich ascites tumor cells on mouse plasma lipoproteins.

In two separate experiments, a total of 120 mice were divided into two equal groups, one a control and the other an experimental group which was injected with Ehrlich ascites tumor cells. In the first experiment, the plasma lipoprotein patterns were obtained by disc electrophoresis from 4 control and 4 Ehrlich ascites tumor cell-injected animals on the 1st, 3rd, 5th, 7th and 9th day after injection of the tumor cells. After the 3rd day of injection, the electrophoretic patterns from Ehrlich ascites tumor-bearing mice indicated a striking decrease in high-density lipoproteins and an elevation in low-density lipoproteins. In order to confirm these results, in a second experiment (40 control and 40 Ehrlich ascites tumor-bearing mice), the plasma high-density lipoproteins were isolated by electrophoretic elution and their phospholipid was determined. A significant decrease (70%, p<0.01) in high-density lipoprotein-phospholipid was observed after the 3rd day of injection in the plasma of tumor-bearing mice and suggested a relationship between the growth of tumor cells and plasma high-density lipoproteins.

The effect of immune organ transplants on homograft rejection in tumor-bearing mice.

Abstract The delayed skin homograft rejection in Ehrlich ascites tumor-bearing mice can be at least partially corrected by specifically immune spleen or thymus transplants. The correction of skin homograft rejection in these studies usually approximated a first set time, not the second set time expected if the tumor were without influence. The transplants did not appear to effect the tumor growth or time of death in these mice. The spleen, thymus, and homograft of the recipient mice, as well as the transplanted spleen and thymus, were examined histologically with acridine orange stained preparations. Increased nuclear and cytoplasmic RNA in both donor and recipient lymphoid cells was observed in the mice receiving immune transplants, skin homografts, or Ehrlich ascites tumor. The possibility that the transfer of RNA or some other subcellular component from competent lymphocytic cells was responsible for the correction of immune response has been discussed.

Some properties of the lactic dehydrogenase agent of mice.

The lactic dehydrogenase agent was obtained in quantities sufficient for purification studies by growing the virus in Ehrlich ascites tumor-bearing mice. A rapid method of titration of the agent is described. Subsequent to the standard procedure of concentration of virus by treatment with hyaluronidase and centrifugation, lipids were removed by extraction with PE, without major loss of infectivity. Electron microscopic sections of purified preparations contained particles consisting of a dense inner ring of about 25 mmicro and a less dense ring extending to about 50 mmicro. The particles occur frequently in single-membraned vesicles of varying size, and occasionally in large double-membraned bodies. The purified LDH agent did not stimulate the formation of neutralizing antibodies in rabbits and guinea pigs. The crude LDH agent was found to be a low interferon producer. Increased interferon, produced by secondary inoculation with Newcastle disease virus temporarily decreased the titer of the LDH agent. The results of others regarding the nature and the size of the LDH agent are interpreted in regard to the findings presented, and the role of interferon in permanently LDH agent infected mice is discussed.