Egyptian Bread Research Articles

Molecular characterization and evaluation of different irrigation regimens on yield and other agronomic traits of some Egyptian wheat cultivars

ABSTRACT The objective of this study was to determine the optimal irrigation needs of six popular Egyptian bread wheat (Triticum aestivum L.) cultivars and to establish baseline effect of reduced irrigation on yield. The varieties included were Sids1, Sids14, Sakha94, Sakha95, Gemmiza12, and Shandawee1. The experiment was conducted at two different locations, Nubaria and Sids Agricultural Research Stations, Egypt over two consecutive seasons, 2020–2021 and 2021–2022. There was a reduction in yield with the decrease in the number of irrigations. However, several cultivars performed better for yield and other traits with one less irrigation than the current irrigation practices in the region. Therefore, the study may help revise irrigation recommendations. Overall, the cultivars Sakha95, Sids1, and Sids14 were taller and had more kernels per spike, higher seed index, and greater grain yield under reduced irrigations and showed the best drought tolerance index. The tested bread wheat cultivars were genotyped using Inter-Retrotransposon Amplified Polymorphism (IRAP) and Start Codon Targeted (SCoT) markers which showed relation among the cultivars. Among the two types of markers, IRAP was better than SCoT markers for reliability and robustness. Among the six cultivars, 58% of the IRAP markers detected polymorphism as compared to 38% with SCoT markers. The two wheat cultivars Sids1 and Sids14, which performed better for important agronomic traits under water stress conditions, could be used as donors for the national wheat breeding program for drought tolerance.

Performance of Some Egyptian Bread Wheat Cultivars under Saline Soil Conditions at North Delta of Egypt.

Performance of Some Egyptian Bread Wheat Cultivars under Saline Soil Conditions at North Delta of Egypt.

Sakha 96:A new early maturing Egyptian bread wheat cultivar

Sakha 96:A new early maturing Egyptian bread wheat cultivar

Wheat Drought Tolerance: Morpho-Physiological Criteria, Stress Indexes, and Yield Responses in Newly Sand Soils

Drought presents a major risk to wheat growth and productivity under changing climates. During the last few years, various morphological and physiological approaches were used to overcome drought stress-associated problems. Cultivation of tolerant wheat cultivars can serve as a sustainable choice to raise wheat yield under water stress. Herein, field trials were carried out at the experimental farm of Ismailia Agricultural Research Station, Egypt, in two successive growing seasons (2020/2021 and 2021/2022) to investigate the response of four Egyptian bread wheat cultivars (Misr 1, Misr 3, Giza 171, and Sakha 95) to drought stress according to morpho-physiological characteristics, yield, and stress indices. Irrigation treatments and cultivars were assigned to the main and sub-plots, consequently, in a split-plot design with three replicates. The findings revealed that in both the first and second seasons, drought drastically revoked growth vigor of shoot, growth vigor of flag leaf, relative water content (%), membrane stability index (%), photosynthetic pigments, heading (days), maturity (days), as well as yield and yield attributes: spike length, number of spikes/m2, spike weight, grain number/spike, 100-kernel weight, grain yield/m2, straw yield/m2, biological yield/m2, and harvest index of all four wheat cultivars. Conversely, drought caused a marked increase in saturation water deficit (%), carotenoids content, and NKP uptake of all four wheat cultivars in both study seasons. The current study found that all four of the wheat cultivars were drought-tolerant plants. These cultivars exhibited similar drought-tolerant behaviors, which included decreased loss in relative water content, membrane stability, and photosynthetic pigment levels, consequently reducing wheat grain yield loss under water stress. Additionally, the drought tolerance indices of Sakha 95 > Giza 171 > Misr 1 > Misr 3 were revealed by the stress sensitivity index (SSI), mean productivity (MP), stress tolerance index (STI), and yield stability index (YSI). In conclusion, Misr 3 was the least tolerant wheat cultivar and Sakha 95 was the most tolerant. These results can be applied to breeding programs by plant breeders.

Effect of some Nitrogen Fertilization Rates on the Yield and Its Components for some New Egyptian Bread Wheat Cultivars

Effect of some Nitrogen Fertilization Rates on the Yield and Its Components for some New Egyptian Bread Wheat Cultivars

Anti-austerity riots in late developing states: Evidence from the 1977 Egyptian Bread Intifada

In late developing states, labor markets are often segmented as a result of import substitution and political coalitions centered on the formally employed. Building on insider–outsider and moral economy frameworks from political economy, we theorize that in such contexts labor market insiders develop strong expectations about welfare provision and public transfers that make them more likely to riot against proposed austerity measures. We test our argument with the case of Egypt during the 1977 Bread Intifada, when the announcement of subsidy cuts sparked rioting across the country. To conduct our analysis, we match an original event catalog compiled from Arabic-language sources with disaggregated employment data. Spatial models, rich micro-level data, and the sudden and short-lived nature of the rioting help us to disentangle the importance of an area’s labor force from its location and wider socio-economic context. As we show, despite the diffuse impact of the subsidy cuts, rioting was especially concentrated in areas with labor market insiders – and this is after accounting for a range of plausible alternative explanations. The results suggest that moral economies arising from labor market segmentation can powerfully structure violent opposition to austerity.

Effect of sowing date on grain yield and quality of some Egyptian bread wheat genotypes

Effect of sowing date on grain yield and quality of some Egyptian bread wheat genotypes

Yield Productivity of Sixteen Egyptian Bread Wheat Varieties Grown under Middle Egypt and West West El-Minya Conditions

Yield Productivity of Sixteen Egyptian Bread Wheat Varieties Grown under Middle Egypt and West West El-Minya Conditions

Study the Effect of Nitrogen Fertilization Rates on Grain Yield and Some Physiological Characteristics of Wheat Varieties

One of the most important strategies for enhancing wheat grain productivity and physiological processes is the application of appropriate nitrogen (N) and the choice of a suitable variety in an adequate environment. The study goals were to assess the impact of various N application rates on the grain yield and some physiological traits of four wheat cultivars under two growing years as well as determine the relationship between these traits across the experimental factors. Four Egyptian bread wheat varieties (Sakha 93, Gemmiza 12, Sids 12, and Sakha 94) and four nitrogen rates including control (N0), 75 kg N fad-1 (N1), 100 kg N fad-1 (N2), and 125 kg N fad-1 (N3) were evaluated in two experimental fields during the 2020/2021 and 2021/2022 growing years at Sakha, Kafr El-Sheikh Governorate, Egypt. Grain yield (kg ha-1), relative water content (RWC) proline content, malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX) activities were the traits under study. The grain yield and the majority of the physiological parameters assessed were significantly influenced by the years, nitrogen treatments, and varieties as well as their first and second-order interactions (p ≤0.05 or 0.01), according to Three-way ANOVA. Grain yield increased with N treatments increased, and N3 treatment was higher than N0, N1 and N3 treatments with values of 31.1%, 22.9%, and 10.6%, respectively. With increased N application there is a tendency for MDA content and SOD activity to decrease and then increase, while RWC, proline content, CAT, and ABX activities to increase and then decrease. The highest values were recorded for grain yield, proline content and CAT activity by Sakha 94 variety and for MDA content, SOD, CAT and ABX activities by Sids 12 variety. According to the interactions among experimental factors, the highest wheat yield and the majority of physiological measures were observed by Sakha 94 variety with N3 rate under the second growing year. The Sakha 94 variety shows a positive correlation with grain production and the majority of physiological parameters under applying N3 treatment during the second growing year based on all variables examined by principal component analysis. Finally, our results recommended using the Sakha 94 variety with 125 kg N fad-1 to increase and improve wheat productivity.

Inheritance as A Base for Future Improvement of New Egyptian Bread Wheat Cultivars

Inheritance as A Base for Future Improvement of New Egyptian Bread Wheat Cultivars

Bread Wheat Productivity in Response to Humic Acid Supply and Supplementary Irrigation Mode in Three Northwestern Coastal Sites of Egypt

Drought stress is a major factor limiting wheat crop production worldwide. The application of humic acid (HA) and the selection of the appropriate genotype in the suitable site is one of the most important methods of tolerance of wheat plants to drought-stress conditions. The aim of this study was achieved using a three-way ANOVA, the stress tolerance index (STI), the Pearson correlation coefficient (rp), and principal component analysis (PCA). Three field experiments in three sites (Al-Qasr, El-Neguilla, and Abo Kwela) during the 2019/21 and 2020/21 seasons were conducted, entailing one Egyptian bread wheat variety (Sakha 94) with three HA rates (0, 30, and 60 kg ha−1) under normal and drought-stress conditions (supplemental irrigation). According to the ANOVA, the sites, supplemental irrigation, HA rates, and their first- and second-order interactions the grain yield and most traits evaluated (p ≤ 0.05 or 0.01) were significantly influenced in both seasons. Drought stress drastically reduced all traits registered in all factors studied compared with normal conditions. The wheat plants at the Al-Qasr site in both seasons showed significantly increased grain yield and most traits compared with that of the other sites under normal and drought-stress conditions. HA significantly promoted all studied traits under drought stress, and was highest when applying 60 kg HA ha−1, regardless of the site. The greatest grain yield and most traits monitored were observed in wheat plants fertilized with 60 kg HA ha−1 at the Al-Qasr site in both seasons under both conditions. Grain yield significantly (p ≤ 0.05 or 0.01) correlated with water and precipitation use efficiency as well as the most studied traits under normal and drought-stress conditions. The results of STI, rp, and PCA from the current study could be useful and could be used as a suitable method for studying drought-tolerance mechanisms to improve wheat productivity. Based on the results of statistical methods used in this study, we recommend the application of 60 kg HA ha−1 to improve wheat productivity under drought conditions along the north-western coast of Egypt.

Evaluation of the morpho-physiological traits and the genetic diversity of some Egyptian bread wheat cultivars under salt stress conditions

Evaluation of the morpho-physiological traits and the genetic diversity of some Egyptian bread wheat cultivars under salt stress conditions

Stability Analysis and Molecular Description of Some Promising Sorghum Lines Tolerant to Salt Stress.

<b>Background and Objective:</b> Salt stress is considering the biggest environmental obstacle to crop productivity, especially sorghum. So, it was necessary to develop new sorghum lines tolerant to salt stress and high yielding to participate in bridging the large gap in the Egyptian bread industry and also as an important feed for animals. This is the biggest goalie this investigation. <b>Materials and Methods:</b> Some promising sorghum genotypes were evaluated under the control experiment and two salinity stress locations to test their stability and its salinity stress tolerance during two years. Some agro-morphological and physiological traits were the most important parameters tested under all conditions besides, 11 SCoT primers for comparing among the seven sorghum genotypes and Identification of molecular genetic markers responsible for salt stress tolerance. <b>Results:</b> The final results revealed that the five promising sorghum lines were recorded highly rank of salinity stress tolerance in all studied traits and a higher level of genetic stability during the two years. <b>Conclusion:</b> Results of agro-physiological traits, salinity tolerance indices and SCoT primers succeed in determining salt stress tolerance mechanisms in sorghum and which an important taxonomic tool is for plant breeder that helps him in sorting the tolerant genotypes from the sensitive ones.

Estimation of Combining Ability and Heterosis for Wheat Yield and Its Components under Water Stress Conditions

DROUGHT is a worldwide problem which controls the crop productivity. Six Egyptian bread wheat varieties were crossed in a 6x6 half diallel in 2018/19 season. Parents and their fifteen F1 crosses were evaluated under normal irrigation (N), mid water stress (MS) and severe water stress (SS) during 2019/20 in three field experiments. Results showed that the analysis of variance were significant for all the studied traits. The highest mean values were recorded under all irrigation treatments as well as the combined analysis with the crosses P1x P2, P1 x P3, P1 x P5, P2 x P3, P3 x P5 and P5 x P6 for grain yield per plant. Mean squares for both general (GCA) and specific (SCA) combining ability estimates were highly significant for all studied traits. GCA/SCA ratio was greater than unity for most traits. The parental P1 exhibited positive and significant GCA effects for number of kernels per spike, grain yield per spike and grain yield per plant. The highest desirable SCA effects were obtained with the crosses P3 x P5 and P4 x P5 for grain yield per plant under stresses conditions. Positive significant heterosis were detected by the crosses P1 x P3, P2 x P3, P3 x P5, P4 x P5 and P5 x P6 for number of spikes per plant, number of kernels per spike, grain yield per spike, 1000-grain weight and grain yield per plant at N, MS, SS conditions and the combined data.

Molecular Evaluation and Identification of Some Barley Hybrids Tolerant to Salt Stress.

<b>Background and Objective:</b> Barley is considering one of the most important cereal crops at the local and global levels. It is ranked second in terms of nutritional importance after wheat and its flour contributes significantly to bridging the large nutritional gap in the production of Egyptian bread. The aim of this study concentrated on knowing and testing the genetic behaviour responsible for salinity stress tolerance in barley as trying to improve barley crop and increase its ability for abiotic stress resistance under Egyptian conditions. <b>Materials and Methods:</b> Twenty-one crosses and ten parents of barley with different responses to salinity tolerance were evaluated in this investigation under normal and salinity conditions. Yield and its components and some physiological traits related to salt stress tolerance were the most important studied attributes evaluated in this regard under both conditions. Moreover, SSR markers were used to evaluate and identified associated markers for salinity tolerance in selected hybrids and comparing among the ten barley parents. <b>Results:</b> The final results confirmed that the three testers; Giza 123, Giza 126 and Giza 2000 besides; the crosses; Line 1XTester 1 (Giza 125XGiza 123), Line 2XTester 1 (Giza 133XGiza 123), Line 1XTester 2 (Giza 125XGiza 126), Line 2XTester 2 (Giza 133XGiza 126) and Line 1XTester 3 (Giza 125XGiza 2000) exhibited highly salinity tolerance under saline stress treatment compared with the control experiment. Among 15 analyzed barley entries, the chosen set of 11 markers amplified 20 alleles with an average of 1.81, with a range from 1-4 alleles. <b>Conclusion:</b> The results of SSR analysis and the data on valued agricultural trait loci determined the genetic distance among parents and their hybrids, which is of an unlimited rate for breeders.

SRAP Molecular Markers Linked to Three Morphological Traits in Egyptian Bread Wheat (Triticum aestivum L.)

Sequence Related Amplified Polymorphism (SRAP) molecular technique was used for assessing the genetic diversity of eight Egyptian bread wheat (Triticum aestivum L.) genotypes. Five SRAP primers combinations were able to expose the polymorphism between the studied genotypes. The percentage of polymorphism (P %) ranged from 40 to 79.90 %. Furthermore, the Polymorphic Information Content (PIC) values for SRAP primers combination varied from 0.11 to 0.31 with an average of 0.19. The ME5F-EM2R and ME1F-EM2R primer combination cleared higher levels of polymorphism of 66.67 and 76.90%, respectively. The results of Single Marker Analysis (SMA) showed that the SRAP marker ME1-EM21320bp could be considered probably as candidate marker linked to spike length in the tested genotypes. Moreover, ME1-EM2900bp and ME1-EM2575bp could be considered as markers linked to plant height and tillers number /plant traits, respectively. Results of Single Marker Analysis (SMA) showed significant marker-trait associations for spike length (P= 0.04), plant height (P= 0.02) and number of tillers/plant (P= 0.03). Me1F-Em6R850bp marker could be also considered as candidate markers probably linked to plant height, with a specific fragment of 850bp for the tolerant genotype (P1). UPGMA cluster analysis based on SRAP markers separated the studied wheat genotypes into three significantly different clusters. The dendrogram based on morphological traits divided the studied wheat genotypes into two different clusters. The Mantel test revealed that there was a positive but non-significant correlation between the genetic similarities based on phenotypic data and SRAP marker (r = 0.36, P> 0.05).

Mining of Leaf Rust Resistance Genes Content in Egyptian Bread Wheat Collection.

Wheat is a major nutritional cereal crop that has economic and strategic value worldwide. The sustainability of this extraordinary crop is facing critical challenges globally, particularly leaf rust disease, which causes endless problems for wheat farmers and countries and negatively affects humanity’s food security. Developing effective marker-assisted selection programs for leaf rust resistance in wheat mainly depends on the availability of deep mining of resistance genes within the germplasm collections. This is the first study that evaluated the leaf rust resistance of 50 Egyptian wheat varieties at the adult plant stage for two successive seasons and identified the absence/presence of 28 leaf rust resistance (Lr) genes within the studied wheat collection. The field evaluation results indicated that most of these varieties demonstrated high to moderate leaf rust resistance levels except Gemmeiza 1, Gemmeiza 9, Giza162, Giza 163, Giza 164, Giza 165, Sids 1, Sids 2, Sids 3, Sakha 62, Sakha 69, Sohag 3 and Bany Swif 4, which showed fast rusting behavior. On the other hand, out of these 28 Lr genes tested against the wheat collection, 21 Lr genes were successfully identified. Out of 15 Lr genes reported conferring the adult plant resistant or slow rusting behavior in wheat, only five genes (Lr13, Lr22a, Lr34, Lr37, and Lr67) were detected within the Egyptian collection. Remarkedly, the genes Lr13, Lr19, Lr20, Lr22a, Lr28, Lr29, Lr32, Lr34, Lr36, Lr47, and Lr60, were found to be the most predominant Lr genes across the 50 Egyptian wheat varieties. The molecular phylogeny results also inferred the same classification of field evaluation, through grouping genotypes characterized by high to moderate leaf rust resistance in one cluster while being highly susceptible in a separate cluster, with few exceptions.

Behavior of Some Egyptian Bread Wheat Genotypes Under Different Natural Photo-thermal Environments

This investigation was conducted during the 2018/2019 and 2019/2020 seasons at Sakha Agricultural Research Station, Egypt to study the influence of four planting dates on earliness and yield of fourteen wheat genotypes. The combined analyses showed highly significant differences due to years, sowing dates, genotypes and their interactions for most studied traits. December 5th was the best sowing date for wheat production. The wheat new genotypes lines 1 and 2 had the lowest days to booting, heading, anthesis and maturity on the other side they had the highest values for grain filling period (GFP) and 1000-kernel weight. Based on the genotype means, the late heading genotype (Giza 163) had short GFP (29.5 days) and possessed a high grain filling rate (233 kg ha-1 days-1). The cultivar Misr 3 was superior overall genotypes for grain yield (10.529 t ha.-1), spikes per square meter (552 spikes), and straw yield (20.164 t ha.-1); and it ranked first under early sowing date (Nov. 5th) with 9% higher grain yield overstudied genotypes. According to stability analyses, Misr 3, Sakha 95 and Giza 171 recorded the highest yield, with the regression coefficient values ˃1. This study recommended release of new wheat genotypes (Lines 1 and 2) as new cultivars and use them to develop early maturing and heat-tolerant bread wheat genotypes in breeding programs.

Efficiency of Yellow Rust Resistance Genes Yr5, Yr10, Yr15 and Yrsp in Improving the Two Egyptian Bread Wheat Cultivars Sids 12 and Gemmeiza 11

A FIELD and greenhouse study was conducted at Sakha Agricultural Research Station during 2015-2020 wheat seasons to enhance stripe rust resistance of the two Egyptian bread wheat cultivars Sids 12 and Gemmeiza 11 using the four monogenic lines Yr5, 10, 15 and Sp. The two wheat cultivars were crossed to the four monogenic lines to obtain eight F1 hybrids then selfed to produce F2 populations and selected F3 families. In the field, parents, F1, F2, F3, and differential genotypes were inoculated with a mixture of predominating pathotypes of the wheat stripe rust pathogen Puccinia striiformis f. sp tritici (Pst). Evaluation of the monogenic lines indicated that wheat genotypes carrying Yr5 and Yr15, at both seedling and adult plant stages exhibited high resistance to the Pst races. F1 field response confirmed that the four tested Yr genes are effective against the tested stripe rust races and resistant reaction is dominating over susceptibility. Segregation ratios of the eight F2 crosses indicated that the cultivars differ in three, two, or one genes with the monogenic lines. Average coefficient of infection recorded the lowest mean values for F2 crosses with Yr5 and Yr15 in both cultivars indicating that the two genes shifted the F2 population means toward resistance more than Yr10 and YrSp. Efficacy of the four genes can be arranged in the following order Yr5 ˃Yr15 ˃YrSp ˃Yr10 with the Sids 12 background and Yr5 ˃Yr15 ˃Yr10 ˃YrSp with the Gemmeiza 11 background. Out of the tested 63 F3 families, the highest percentage of completely resistant plants were recorded with the Yr5 crosses (35-40%) followed by Yr10 cross (34%) with Gemmeiza 11 and then the Yr15 cross (26%) with Sids 12. Two of the F3 families from Yr5 crosses were phenotypically closer to the recipient cultivars that were completely resistant and hence may have Yr5 gene in homozygous state. The promising resistant lines derived from both cultivars will be evaluated for yield and quality characteristics during the next season. Based on our results, pyramiding combinations of the three effective genes Yr5, Yr10 and Yr15 in one wheat background is expected to enhance resistance for the dominating stripe rust races in Egypt.

Rheological Assessment of Different Bread Wheat Genotypes Induced via radiation and Hybridization

The study aimed to evaluate the physicochemical and rheological properties of flour samples for six commercial Egyptian bread wheat (two commercial varieties namely Giza-168 and Gemmeiza-11, and four promising lines namely L84, L148, M10, and M34). The sample of M10 which induced/mutated by gamma radiation at dose 350 Gy, showed the best physicochemical and rheological properties, i.e., ash, protein, wet gluten, gluten index, falling number, extraction percentage, and Zeleny value were 0.63, 14.1 %, 36.4 %, 87%, 567 sec., 83.8% and 58 %, respectively. The farinograph parameters water absorption (65.8%), dough development time (11 min) and dough stability (10.8 min) were significantly (P < 0.05) higher in M10 compared to all other samples under study. Furthermore, alveograph parameters showed the highest tenacity (142 mm), dough extensibility (107 mm) and dough deformation energy (499 10-4J). Thus, the promising sample induced via gamma radiation (M10) was the best for parameters in bread making.