Chicken Eggs Research Articles

Fecal bacterial microbiota diversity characterized for dogs with atopic dermatitis: its alteration and clinical recovery after meat-exclusion diet.

To achieve clinical recovery in canine atopic dermatitis affected pet dogs via alteration of the gut microbiome, following a meat and egg exclusion diet for 60 days. 24 atopic dermatitis-affected pet dogs, all fed poultry meat and egg, and another 48 apparently healthy controls fed both poultry meat and egg (n = 24) or vegetable diet (24) were included in the study. The study was undertaken in the Bhubaneswar Smart City, Odisha, India, from July to December 2023. Fecal samples were collected at 2 points for DNA analysis, ie, on day 0 and day 60 of the change from a meat/egg-based diet to a vegetable-based diet. Extracted DNA samples were pooled category-wise and subjected to the gut microbiome analysis in the Nanopore sequencer targeting the 16S rRNA gene. Burrows-Wheeler Transform, Ferragina-Manzini index, and Krona charts were used for taxonomical classification and visualization of relative abundances of bacterial species within the metagenome. Alpha- and beta-diversity analyses were performed. Atopic pets at day 0 showed elevation in the gut microbiome population with an adequate concentration of pathogens like Escherichia coli and Clostridiodes difficile with lower amounts of the beneficial bacteria like Lactobacillus sp, while the pets at 60 days after dietary intervention showed a significant decline in bacterial species like E coli and C difficile with higher amount of Lactobacillus sp. Both control groups showed variations of microbiome between them as well as from the atopic pets. We found a close association of poultry meat/egg diet with gut microbiome population and atopic symptoms as well in dogs, and elimination of such diet could be helpful in clinical recovery. Dietary intervention with the exclusion of potential allergens from poultry meat and egg sources can be an effective approach for the management of canine atopic dermatitis.

Precision Monitoring of Dead Chickens and Floor Eggs with a Robotic Machine Vision Method

Modern poultry and egg production is facing challenges such as dead chickens and floor eggs in cage-free housing. Precision poultry management strategies are needed to address those challenges. In this study, convolutional neural network (CNN) models and an intelligent bionic quadruped robot were used to detect floor eggs and dead chickens in cage-free housing environments. A dataset comprising 1200 images was used to develop detection models, which were split into training, testing, and validation sets in a 3:1:1 ratio. Five different CNN models were developed based on YOLOv8 and the robot’s 360° panoramic depth perception camera. The final results indicated that YOLOv8m exhibited the highest performance, achieving a precision of 90.59%. The application of the optimal model facilitated the detection of floor eggs in dimly lit areas such as below the feeder area and in corner spaces, as well as the detection of dead chickens within the flock. This research underscores the utility of bionic robotics and convolutional neural networks for poultry management and precision livestock farming.

Evaluation of mucosal adjuvants to chitosan-nanoparticle-based oral subunit vaccine for controlling salmonellosis in broilers

Salmonellosis, a gastrointestinal disease, continues to be one of the major public health concerns worldwide. Poultry meat and eggs are recognized as the major source of Salmonella food poisoning in humans. Our study evaluated the protective efficacy of mannose-conjugated chitosan-nanoparticle (mChitosan-NP)-based subunit vaccine, consisting of immunogenic outer membrane proteins and flagella of Salmonella Enteritidis [mChitosan (OMP+FLA)/FLA-NP], coadministered orally with potent mucosal adjuvants to reduce the colonization of S. Enteritidis in the intestines of broiler chickens. We evaluated the adjuvant effects of three different doses of two well-known mucosal adjuvants, c-di-GMP (stimulator of interferon gene agonist) and whole cell lysate (WCL) of Mycobacterium smegmatis, to improve the efficacy of mChitosan (OMP+FLA)/FLA-NP vaccine. Our data reaffirmed the potent adjuvanticity of both of these adjuvants and identified their optimal dose when entrapped in mChitosan-NP to potentiate the immunogenicity and efficacy of orally delivered mChitosan (OMP+FLA)/FLA-NP vaccine. The physical characteristics of mChitosan (OMP+FLA)/FLA-NP, mChitosan-GMP/FLA-NP, and mChitosan-WCL/FLA-NP formulations revealed a high positive charge (Zeta potential +20–25 mV), size 235–260 nm, and polydispersity index 0.35–0.52, which are conducive for oral delivery. The efficacy in chickens that received oral administration with a combination of the vaccine-adjuvant formulations was evaluated by challenging with Salmonella Enteritidis. Our data showed that mChitosan (OMP+FLA)/FLA-NP WCL at 10 µg/dose formulation consistently reduced the S. Enteritidis load by over 0.5 log10 comparable to a commercial live vaccine at post-challenge days 4 and 10. Immunologically, we observed enhanced systemic and mucosal antibody and cellular (B cells and T-helper cells) immune responses as well as upregulation of expression of immune cytokine genes IFN-γ, TGF-β, and IL-17 in the cecal tonsils of adjuvanted mChitosan-NP Salmonella-subunit-vaccinated birds. Overall, particularly the mucosal adjuvant WCL consistently enhanced the efficacy of mChitosan (OMP+FLA)/FLA-NP vaccine by inducing effective immune responses.

The State of poultry industry in Belarus at the present stage

According to analysts, due to increased consumer demand, from the beginning of the 21st century, poultry production will increase many times by 2030. The planned regulated economy of Belarus, strict adherence to technological norms and rules, uninterrupted supply of poultry with complete feed and high personal responsibility of labor collectives have made it possible to achieve high levels of egg and poultry meat production. The share of the meat market in Belarus is 39.0% owned by poultry products, which have displaced such industries as pig breeding, horse breeding and sheep breeding. With a relatively low number of farm birds of 49.9 million birds, it became possible to achieve the following high results. Meat production per capita in 2023 was 139 kg, with consumption of 101 kg, i.e. an additional 27.3% of meat was produced. At the same time, of the indicated volume, 54.5 kg is poultry meat. The annual overproduction of meat at the level of 20-30% allows us to have additional profits from its sale in markets near and far abroad. A similar situation is developing in egg poultry farming. Over the past year, the production of edible eggs per capita in Belarus amounted to 375 pieces, but their consumption does not exceed 272 pieces. Additional produced products in the amount of 27.5% were sold, first of all, to the market of the Russian Federation, and then to China and other countries. The selection of countries for poultry exports was primarily based on statistical information on per capita production levels. Thus, timely analysis of statistical information makes it possible to regulate investments and predict the profitability of the production of eggs and poultry meat and, if necessary, promptly provide producers with new sales markets without allowing excessive overproduction of livestock (poultry) products.

Keberadaan Bakteri Salmonella sp Pada Telur Ayam Di Pasar Tradisional Kota Palembang Sumatera Selatan

Eggs are a product of animal origin that is famous as a high source of protein compared to other protein source ingredients. Damage that can cause a decrease in egg quality is caused by microorganisms, one of which, bacteria that can contaminate eggs is Salmonella sp. This study aims to analyze the presence of Salmonella sp bacteria in chicken eggs sold in several traditional markets in Palembang City. This study used 6 samples of chicken eggs from 3 traditional markets with a simple random sampling method. The test results of all positive egg samples contained Salmonella sp. This shows that the quality of eggs sold at the Palembang Traditional Market does not meet the requirements of the Indonesian National Standard, which is negative/25 grams. Keywords: Salmonella sp, chicken egg, traditional market.

Comparative Patho-Genomics of Salmonella enterica Serovar Enteritidis Reveal Potential Host-Specific Virulence Factors

Salmonella enterica serovar Enteritidis (S. Enteritidis) is one of the most common causes of bacterial foodborne infections worldwide. It has an extensive host range, including birds and humans, making it one of the most adaptable Salmonella serovars. This study aims to define the virulence gene profile of S. Enteritidis and identify genes critical to its host specificity. Currently, there is limited understanding of the molecular mechanisms that allow S. Enteritidis to continue as an important foodborne pathogen. To better understand the genes that may play a role in the host-specific virulence and/or fitness of S. Enteritidis, we first compiled a virulence gene profile-based genome analysis of sequenced S. Enteritidis strains isolated from shell eggs in our laboratory. This analysis was subsequently used to compare the representative genomes of Salmonella serovars with varying host ranges and S. Enteritidis genomes. The study involved a comprehensive and direct examination of the conservation of virulence and/or fitness factors, especially in a host-specific manner—an area that has not been previously explored. Key findings include the identification of 10 virulence-associated clusters of orthologous genes (COGs) specific to poultry-colonizing serovars and 12 virulence-associated COGs unique to human-colonizing serovars. Virulence/fitness-associated gene analysis identified more than 600 genes. The genome sequences of the two S. Enteritidis isolates were compared to those of the other serovars. Genome analysis revealed a core of 2817 COGs that were common to all the Salmonella serovars examined. Comparative genome analysis revealed that 10 virulence-associated COGs were specific to poultry-colonizing serovars, whereas 12 virulence-associated COGs were present in all human-colonizing serovars. Phylogenetic analyses further highlight the evolution of host specificity in S. Enteritidis. This study offers the first comprehensive analysis of genes that may be unique to and possibly essential for the colonization and/or pathogenesis of S. Enteritidis in various and specific hosts.

Determination of blood biochemical indices and research of egg quality-related candidate gene CDH5 in Putian black duck

Improving egg quality and enhancing production efficiency are essential goals in poultry breeding. CDH5 encodes a cadherin involved in Ca2+ transport in endothelial cells. The role of CDH5 in regulating duck egg quality and its mechanisms affecting Ca2+ concentrations in duck uterine epithelial cells remain unclear. This study evaluated egg quality traits of the Putian black duck and conducted an association analysis with blood biochemical indices and single nucleotide polymorphisms in CDH5. Additionally, we constructed a CDH5 overexpression vector and synthesized specific siRNAs for transfection into Putian black duck uterine epithelial cells to assess Ca2+ concentrations. Our results revealed significant association between egg quality and five novel SNPs in CDH5, along with various blood biochemical indices. Further experiments demonstrated that CDH5 overexpression and knockdown reduced Ca2+ concentrations in the uterine epithelial cells of Putian black ducks.

Screening of Brinjal Varieties for Resistance Against Blight Disease Caused by Alternaria alternata (Fr.) Keissler in Konkan Region of Maharashtra, India

Brinjal (Solanum melongena L.) belonging to family solanaceae, is widely recognized around the globe as “eggplant” due to it’s resemblance to the shape of a chicken egg. The field experiment was carried out during Rabi, 2022-23 and 2023-24 at Central Experiment Station, Wakawali, Dr. Balasaheb sawant Konkan Krishi Vidyapeeth, Dapoli under natural epiphytotic conditions. Sixty six varieties / cultivars and germplasm lines of brinjal were screened to test their resistance reactions against blight disease of brinjal caused by Alternaria alternata. The results revealed that, all the entries of brinjal screened showed varied degree of per cent disease intensity. The highest per cent disease intensity was recorded in Konkan Prabha (41.92%) and was followed by NB-746 (37.91%), Dapoli local-1 (34.62%) and Dapoli local-3 (33.42%). Among 66 entries screened, three entries viz., D-79-19, RCMB-3 and Dapoli pandharivangi sel. were found highly resistant, seven entries viz., Khed local-41, DPL-BR-19, NBH-106, Suwarna Pratibha, DPL-BR-20, N-1007 and NBH-104 resistant, thirty eight entries moderately resistant and eighteen entries of brinjal were moderately susceptible to A. alternata incitant of brinjal blight disease.

The Manufacture and Evaluation of a Local Incubator Performance for Hatching Poultry Eggs

The Manufacture and Evaluation of a Local Incubator Performance for Hatching Poultry Eggs

Utilizing Chicken Egg White and L-Cysteine for Green Synthesis of Carbon Dots: Rapid and Cost-Effective Detection of Cu2+ Ions

Utilizing Chicken Egg White and L-Cysteine for Green Synthesis of Carbon Dots: Rapid and Cost-Effective Detection of Cu2+ Ions

P-2364. Estimated Additional Burden Averted for the 2022-2023 influenza season from Use of Cell-Based Influenza Vaccines Compared to Egg-Based Influenza Vaccines Among People 0-64 Years of Age in the United States

Abstract Background Estimation of burden averted is useful to contextualize the impact of differentiated vaccines. Traditional influenza vaccine production in eggs can introduce egg-adaptive mutations. Cell-based influenza vaccines avoid egg-adaptation, potentially improving match to circulating influenza viruses and thereby vaccine effectiveness (VE). This study modeled the public health impact if all people aged 0-64 years vaccinated in the United States during the 2022-2023 influenza season had received either cell-based inactivated quadrivalent influenza vaccine (ccIIV4) or egg-based inactivated quadrivalent influenza vaccine (IIV4).Table 1:Number of outcomes prevented from use of IIV4 and ccIIV4, individuals 0—64 Years Methods The modeling method used by the US Centers for Disease Control and Prevention (CDC) to estimate overall burden averted due to influenza vaccination was extended to a relative VE (rVE) context. The model utilized 2022-2023 CDC data on influenza vaccine uptake, influenza incidence, influenza-related healthcare resource (HRU) use and deaths. CDC estimates of absolute VE (aVE) (any vaccine) were used as the aVE of IIV4. We applied an rVE of 7.7% (0.9 to 13.9%), estimated in a 2022-2023 retrospective test-negative design study. Base-case results were validated with deterministic (DSA) and probabilistic (PSA) sensitivity analyses.Table 2:Number of additional outcomes prevented from use of ccIIV4 compared to IIV4, by age group Results Table 1 shows the anticipated number of symptomatic influenza cases and associated HRU and deaths that would have been averted if all vaccinated people 0-64 years of age received either ccIIV4 or IIV4 and Table 2 shows the additional burden averted by age. Use of ccIIV4 would result in prevention of an additional 622,826 symptomatic illnesses, 307,682 outpatient visits, 3,680 hospitalizations, 559 intensive care unit (ICU) admissions, and 127 deaths compared to IIV4. DSA results showed that the rVE estimate was associated with the most variability, followed by influenza-related HRU and deaths. Mean PSA results were all within a 0.1% difference of base-case results. Conclusion Use of ccIIV4 instead of IIV4 during the 2022-2023 influenza seasons in the US would have had a substantial public health impact on the population 0-64 years of age due to prevention of an additional 622,826 symptomatic illnesses as well as proportionate reductions in associated HRU and deaths. Disclosures Ian McGovern, MPH, CSL Seqirus: Employee|CSL Seqirus: Stocks/Bonds (Public Company) Alicia N. Stein, MBiostats, PhD, CSL Seqirus: Employee|CSL Seqirus: Stocks/Bonds (Public Company) Mendel Haag, PhD, PharmD, CSL Seqirus: Employment|CSL Seqirus: Stocks/Bonds (Public Company)

Management Practices at Poultry Demonstration Centres for Boosting Backyard Poultry Farming System in Ramban District of Jammu & Kashmir, India: A Review

The Backyard poultry farming is an age-old practice in India and plays an important role to reduce poverty among the most under-privileged sections of the Jammu & Kashmir, particularly in far flung areas of rural J&K, India. There is not any specific arrangement required for backyard poultry farming and is mostly popular in backward and resource-deprived areas of J&K and provides rural families with good source of income, healthy food sources in terms of meat and eggs. Backyard poultry farming improves the status of women in rural communities, creates jobs for rural residents, and closes the gap between supply and demand for chicken products, such as meat and eggs. The high mortality rate among chicks due to frequent disease outbreaks, lack of a framework, lower potential of Desi chickens, lack of scientific information, predation, hunger, climate change, and year-round fluctuations in feed supply and price are some of the major drawbacks of backyard poultry farming in J&K. This farming needs to be improved because it is the best way for J&K's marginal and small farmers to supplement their income with relatively little investment. Nonetheless, the J&K government has started 29 projects under the Holistic Agriculture growth Programme (HADP), of which P25 is only focused on the growth of poultry in J&K. Backyard poultry farming results in socio-economic development, nutritional and livelihood security, empowerment of women etc. among rural people. Birds are kept mostly on scavenging system, where they acquire their feeds through natural foraging, kitchen wastes etc. Rural poultry farming has been responsible to produce 40 per cent meat and 44 per cent egg requirement in J&K. Chicken eggs and meat are the only cheapest and best sources of protein, vitamin and minerals with high biological value for rural poor and marginal sections of people. The additional income earned from indigenous chicken farming has improved their socio- economic stability.

Morphology-Based In-Ovo Sexing of Chick Embryos Utilizing a Low-Cost Imaging Apparatus and Machine Learning

The routine culling of male chicks in the laying hen industry raises significant ethical, animal welfare, and sustainability concerns. Current methods to determine chick embryo sex before hatching are costly, time-consuming, and invasive. This study aimed to develop a low-cost, non-invasive solution to predict chick embryo sex before hatching using the morphological features of eggs. A custom imaging apparatus was created using a smartphone and light box, enabling consistent image capture of chicken eggs. Egg length, width, area, eccentricity, and extent were measured, and machine learning models were trained to predict chick embryo sex. The wide neural network model achieved the highest accuracy of 88.9% with a mean accuracy of 81.5%. Comparison of the imaging apparatus to a high-cost industrial 3D scanner demonstrated comparable accuracy in capturing egg morphology. The findings suggest that this method can contribute to the prevention of up to 6.2 billion male chicks from being culled annually by destroying male embryos before they develop the capacity to feel pain. This approach offers a feasible, ethical, and scalable alternative to current practices, with potential for further improvements in accuracy and adaptability to different industry settings.

Manufacturing of a New Portland cement from chicken eggshell waste

The approach recommended in this study is to develop an economically feasible and environmentally friendly cement, by partial replacement of the clinker with artificial pozzolans without affecting the performance of the cement. These artificial pozzolans are obtained by heat treatment of residual waste from animal by-products, in this case, chicken egg shells. These materials, after calcination at 750°C, acquire a large presence of lime (CaO) which allows them to react easily with the silica SiO2 contained in the clinker. In the present study, the calcined powder of CESA chicken eggshells is used as a substitute for clinker in different proportions (15%, 30% and 45%) as a replacement for ordinary Portland cement (OPC). The results obtained after calcination at 750°C, a better pozzolanicity, synonymous with the presence of calcite CaCO3.

Utilization of Chicken Egg Shells as Adsorbents for Indigosol Blue Dyes with Variations of Activators

The textile industry still has serious problems in managing waste disposal that causes environmental pollution, including the batik factory industry. Many studies have been conducted to overcome ecological damage by reducing the levels of dyes contained in textile industry waste. Apart from textile industry waste, household waste, especially egg shells, is very high. One alternative method for processing batik factory liquid waste is by adsorption using biosorbent, which is considered cheaper and easier to obtain. An example of a biosorbent is an eggshell. This study includes the characterization of eggshells activated by NaOH and HCl for Indigosol Blue adsorption, morphological analysis of eggshells with SEM and EDS, wavelength determination, calibration curve, and adsorption capacity. The morphology of the eggshell shows a porous surface with high levels of calcium, oxygen, and carbon, indicating that the main compound forming the eggshell is CaCO3. The determination of maximum wavelength and adsorption capacity is done using the UV-Vis spectrophotometry method. The maximum wavelength is 530 nanometers, and an R2 value of 0.9596 was obtained from the regression curve equation. Characterization according to SNI eggshell before activation water content: 0.34%, ash content: 0.25%, iodine absorption: 326.552mg/gram and after activation with NaOH water content is 1.34%, ash content: 0.39%, iodine absorption: 403.015mg/gram. The optimal concentration of processed egg shells for indigosol blue adsorbent is 2% egg shells, the largest in this study as an adsorbent. It turns out that adsorption is still ongoing. The optimum type of activator for Indigosol Blue absorption is 100Mesh dry eggshell powder from NaOH activation.

Determination of the Pathogenicity of the Variant UPM 1432/2019 IBDV in SPF Chicken Eggs and Chicken Fibroblast Cell Line

The infectious bursal disease virus (IBDV) is a nuisance to chicken productivity in the global poultry industry due to its high pathogenicity. In this study, the pathogenicity of the UPM 1432/2019 variant was evaluated using specific pathogen-free (SPF) chicken eggs and fibroblast cell lines. This viral strain was isolated from chickens displaying symptoms of watery diarrhoea and ruffled feathers. Following inoculation, the SPF chicken eggs, and cell lines were meticulously monitored for indicators of viral replication and the onset of cytopathic effects (CPE). The results showed that the virus could replicate in the SPF eggs and cell lines due to the presence of CPE in some cells. In addition, virus-induced weight loss in infected embryonated chicken eggs was evaluated. The UPM 1432/2019 and UPM 1219/2019 strains demonstrated similar mortality rates and CPE features. These findings indicate that the UPM 1432/2019 variant is pathogenic in SPF chicken eggs and fibroblast cell lines. Furthermore, the new UPM 1432/2019 variant caused IBD with pathogenic characteristics similar to the traditional UPM 1219/2019 variant. The emergence of the UPM 1432/2019 variant poses a risk to the economy and poultry production and is potentially resistant to the existing bursal disease vaccines. Consequently, the UPM 1432/2019 variant may significantly contribute to economic losses for chicken farm operators. Moreover, this study provides essential information on the potential virulence of the UPM 1432/2019 variant of IBDV and its potential impact on the poultry industry.

Development of a Solar-Powered Incubator for Poultry Eggs

The development of solar-powered egg incubators holds promise for sustainable agriculture and poultry farming, particularly in areas with limited access to reliable electricity. Hence, in this work, locally sourced materials were used for the development of solar-powered incubator, with the aim of providing a sustainable and reliable alternative to traditional electricity-powered incubators. The design of the incubator was carried out using the different equations stipulated in literatures. Also, the performance of the incubator was investigated by determining the system temperature, relative humidity, percentage fertility and hatchability. The experimental results indicated that the total heat generated inside the incubator was 149,753.49 J, with contributions from air, eggs, water, and plywood. Also, there was consistent temperature and relative humidity readings throughout the incubation period with the temperature and relative humidity falling between 37.1-38.0°C and 50.6-56% respectively. The study also showed that the incubation process was conducted with diligence, leading to favorable outcomes (67% fertility and 75% hatchability) for embryo development and hatching success.

Analysis of Shell Egg Pasteurization Using Computational Fluid Dynamics

In the present work, the thermal pasteurization process of shell eggs was studied through Computational Fluid Dynamics. For large-size eggs (63–72 g), the required heating times at several different heating medium (water) temperatures, namely 54, 56, 58, and 60 °C (followed by cooling in water at 20 °C), were estimated as 55.1, 37.2, 29.1, and 24.5 min. Calculations were based on a 5-log reduction of the Salmonella enteritidis population, that is, for a target F value at 60 °C in 0.85 min. Computations were performed at the critical point of the egg, which was established from F value distribution determinations and was approximately located at the center of the yolk. Both heating and cooling cycle lethality was considered. Similar calculations were performed for medium (53–62 g) and extra-large-size eggs (>72 g). Microbial destruction occurring during the cooling cycle of the pasteurization process was greater for higher heating temperatures and larger egg sizes, ranging between 11.8% of the total destruction for the medium eggs heated at 54 °C and 72.9% for the extra-large eggs at a heating medium temperature of 60 °C. Therefore, destruction during the cooling cycle should not be ignored during the design of thermal pasteurization processes. For the kinetic parameters employed, quality degradation calculations revealed minimal changes for the processes investigated.

Growth-Inhibitory Effect of Chicken Egg Yolk Polyclonal Antibodies (IgY) on Zoonotic Pathogens Campylobacter jejuni, Salmonella spp. and Escherichia coli, In Vitro

The overuse of antibiotics in animal husbandry has driven the search for alternative strategies to combat zoonotic pathogens. Foodborne zoonotic diseases caused by pathogenic bacteria pose a significant threat to human health, and therefore food safety should be a priority. This study investigates the in vitro inhibitory effects of chicken egg yolk immunoglobulin Y (IgY) on the growth and viability of three major foodborne pathogens: Campylobacter jejuni, Salmonella spp., and Escherichia coli. IgY was isolated from immunized hen egg yolks using a modified water dilution method, and its antigen-specificity confirmed through agglutination assays. Growth inhibition was evaluated across multiple doses and time points, revealing a dose-dependent bacteriostatic effect against all tested pathogens. A single dose of IgY (0.5 mg/mL) significantly reduced C. jejuni counts by up to 7 log, while repeated doses were required for Salmonella spp. and E. coli. These findings highlight egg yolk immunoglobulin’s potential as a source of sustainable, effective, ethical, readily available, and inexpensive antibiotic substitutes in livestock management. Future research will focus on validating these results in vivo and exploring large-scale production of IgY for practical application in animal healthcare.

Experimental Investigation into the Design, Optimization, Toxicity, and Anti-Viral Efficacy of Proliposomes Loaded with Ivermectin Against Infectious Bronchitis Virus Using an Embryonated Chicken Egg Model

Background: Infectious bronchitis virus (IBV) causes a significant illness in birds, making it a leading source of financial loss in the poultry business. The objective of this study was to assess the effectiveness of proliposomes (PLs) containing ivermectin (IVM) against IBV using embryonated chicken eggs (ECEs). Methods: A three-factor, two-level (23) full factorial design was employed; carrier/lipid phase ratio (A), stearyl glycyrrhetinate amount (B), and phospholipid type (C) were studied as independent variables, while product yield (PY), entrapment efficiency (EE), particle size (PS), polydispersity index (PDI), zeta potential (ZP), and cumulative percentage of drug released after 6 h (Q6h) were characterized. The selected formulations (PL2 and PL6) were subjected to further characterizations, including IVM toxicity and anti-viral activity. Results: The PY% ranged from 88.6 ± 2.19% to 98.8 ± 0.45%, EE% was from 71.8 ± 2.01% to 96.1 ± 0.51%, PS was from 330.1 ± 55.65 nm to 1801.6 ± 45.61 nm, PDI was from 0.205 ± 0.06 to 0.603 ± 0.03, ZP was from −18.2 ± 0.60 mV to −50.1 ± 1.80 mV, and Q6h was from 80.95 ± 1.36% to 88.79 ± 2.03%. IVM-loaded PLs had lower toxicity in ECEs than pure IVM; the mortality rate was substantially reduced in IBV-infected ECEs injected with PL2 rather than pure IVM. As further evidence of IVM’s anti-viral action against IBV, quantitative real-time polymerase chain reaction (qRT-PCR) revealed that the PL2-treated group exhibited further reduction in IBV’s copies in comparison with the pure IVM-treated group. Conclusions: PLs loaded with IVM are an innovative and potentially effective way to inhibit IBV.