Research Efforts Research Articles

Cortical areas associated to higher cognition drove primate brain evolution

Although intense research effort is seeking to address which brain areas fire and connect to each other to produce complex behaviors in a few living primates, little is known about their evolution, and which brain areas or facets of cognition were favored by natural selection. By developing statistical tools to study the evolution of the brain cortex at the fine scale, we found that rapid cortical expansion in the prefrontal region took place early on during the evolution of primates. In anthropoids, fast-expanding cortical areas extended to the posterior parietal cortex. In Homo, further expansion affected the medial temporal lobe and the posteroinferior region of the parietal lobe. Collectively, the fast-expanding cortical areas in anthropoids are known to form a brain network producing mind reading abilities and other higher-order cognitive functions. These results indicate that pursuing complex cognition drove the evolution of Primate brains.

Role of Population Based Studies in Advancing our Knowledge of Myelodysplastic Syndromes.

Myelodysplastic syndromes (MDS) are myeloid neoplasms characterized by high molecular and genomic heterogeneity. Accordingly, efforts in risk assessment and therapeutic intervention mostly target unique profiles that individualize specific MDS subtypes. In this review, we explored the contributions of population based studies accounting for MDS as a group. Large population based studies have been critical to define important details of our current knowledge of the disease. We summarized the most important population research contributions in MDS, focusing on its epidemiology, population risk factors, and relevant clinical associations. We discuss how these population data can provide vital insights to inform prevention measures, testing strategies, and treatment decisions. Population studies play an important role in guiding clinical and research efforts in MDS. Despite its complex molecular and genomic landscape, population data is integral to define the burden of disease, identify risk factors and clinical associations, and can help elucidate pathogenic mechanisms.

Single-Larva RNA Sequencing Reveals That Red Sea Urchin Larvae Are Vulnerable to Co-Occurring Ocean Acidification and Hypoxia.

Anthropogenic carbon dioxide emissions have been increasing rapidly in recent years, driving pH and oxygen levels to record low concentrations in the oceans. Eastern boundary upwelling systems such as the California Current System (CCS) experience exacerbated ocean acidification and hypoxia (OAH) due to the physical and chemical properties of the transported deeper waters. Research efforts have significantly increased in recent years to investigate the deleterious effects of climate change on marine species, but have not focused on the impacts of simultaneous OAH stressor exposure. Additionally, few studies have explored the physiological impacts of these environmental stressors on the earliest life stages, which are more vulnerable and represent natural population bottlenecks in organismal life cycles. The physiological response of the ecologically and commercially important red sea urchin (Mesocentrotus franciscanus) was assessed by exposing larvae to a variety of OAH conditions, mimicking the range of ecologically relevant conditions encountered currently and in the near future along the CCS. Skeleton dissolution, larval development, and gene expression show a response with clearly delineated thresholds that were related to OAH severity. Skeletal dissolution and the induction of Acid-sensing Ion Channel 1A at pH 7.94/5.70 DO mg/L provide particularly sensitive markers of OAH, with dramatic shifts in larval morphology and gene expression detected at the pH/DO transition of 7.71/3.71-7.27/2.72 mg/L. Experimental simulations that describe physiological thresholds and establish molecular markers of OAH exposure will provide fishery management with the tools to predict patterns of larval recruitment and forecast population dynamics.

Toward Sustainable Polydienes.

The sustainable management of polydiene waste represents a formidable challenge in the realm of polymer chemistry, given the extensive industrial utilization of polydienes due to their superior elastomeric properties. This comprehensive Perspective addresses the multifaceted obstacles hindering efficient recycling of polydienes, encompassing environmental concerns, technical limitations, and economic disincentives. We systematically dissect the influence of polydienes' chemical structures on their recyclability, tracing the evolution of polydiene utilization and disposal practices while assessing the current landscape of waste management strategies. Our investigation reveals the primary technical challenges associated with polydiene recycling, notably the energy-intensive nature of modification processes and the environmental detriments of prevailing disposal techniques. Furthermore, we critically evaluate existing recycling methodologies─including mechanical recycling, energy recovery, and chemical recycling─highlighting their respective merits, constraints, and environmental implications. Pioneering advancements in recycling technology, such as topochemical polymerization and computational prediction models, are spotlighted for their potential to revolutionize polydiene recycling. Looking forward, we delineate an optimistic trajectory for polydiene waste management, advocating for innovative polymerization methods, the exploration of milder recycling conditions, and the adoption of interdisciplinary approaches to bolster recycling efficiency. The Perspective culminates in a discussion on the pivotal role of policy frameworks, life cycle assessments, and economic analyses in shaping the future of polydiene recycling. Through this scholarly examination, we aim to catalyze further research and development efforts aimed at mitigating the environmental impact of polydiene waste, thereby contributing to the broader objective of sustainable chemistry.

Remote Mapping of Bedrock for Future Cosmogenic Nuclide Exposure Dating Studies in Unvisited Areas of Antarctica

Cosmogenic nuclide exposure dating is an important technique for reconstructing glacial histories. Many of the most commonly applied cosmogenic nuclides are extracted from the mineral quartz, meaning sampling of felsic (silica-rich) rock is often preferred to sampling of mafic (silica-poor) rock for exposure dating studies. Fieldwork in remote regions such as Antarctica is subject to time constraints and considerable logistical challenges, making efficient sample recovery critical to successful research efforts. Remote sensing offers an effective way to map the geology of large areas prior to fieldwork and expedite the sampling process. In this study, we assess the viability of multispectral remote sensing to distinguish felsic from mafic rock outcrops at visible-near infrared (VNIR) and shortwave infrared (SWIR) wavelengths using both the Advanced Spaceborne Thermal Emission and Reflection Radiometer (ASTER) and very high-resolution Worldview-3 (WV-3) imagery. We applied a combination of spectral mapping and ground truth from spectral measurements of 17 rock samples from Mount Murphy in the Amundsen Sea sector of West Antarctica. Using this approach, we identified four dominant rock types which we used as a basis for felsic–mafic differentiation: felsic granites and gneisses, and mafic basalts and fragmental hydrovolcanic rocks. Supervised classification results indicate WV-3 performs well at differentiating felsic and mafic rock types and that ASTER, while coarser, could also achieve satisfactory results and be used in concert with more targeted WV-3 image acquisitions. Finally, we present a revised felsic–mafic geological map for Mt Murphy. Overall, our results highlight the potential of spectral mapping for preliminary reconnaissance when planning future cosmogenic nuclide sampling campaigns in remote, unvisited areas of the polar regions.

Identification of novel KRASG12D neoantigen specific TCRs and a strategy to eliminate off-target recognition

BackgroundT cell receptor (TCR)–engineered T cells targeting neoantigens originated from mutations in KRAS gene have demonstrated promising outcomes in clinical trials against solid tumors. However, the challenge lies in developing tumor-specific TCRs that avoid cross-reactivity with self-antigens to minimize the possibility of severe clinical toxicities. Current research efforts have been put towards strategies to eliminate TCR off-target recognition.MethodsNaive T cell repertoire was used for screening KRASG12D-reactive TCRs. Specific TCRs were subsequently identified and their functionality was assessed using TCR Jurkat cells and TCR T cells. Peptide specificity was evaluated using the X-scan assay. To enhance TCR specificity for KRASG12D and reduce their reactivity to self-peptide SMC1A29-38, mammalian TCR display libraries were employed for the design of modification in the complementarity-determining region (CDR).ResultsHLA-A*11:01-restricted TCRs targeting the KRASG12D epitope were isolated, and TCR1 was characterized with superior functional avidity and specificity. Alongside a robust recognition of endogenous KRASG12D epitope, this TCR displayed cross-reactivity with the SMC1A29-38 epitope. With an approach utilizing structural-guided mutations in the CDR-1A region of TCR1, we obtained an engineered TCR variant (TCR1a7). Functional characterization of TCR1a7 showed that this TCR not only exhibited enhanced specificity towards KRASG12D, but also demonstrated successful elimination of the off-target recognition of SMC1A29-38.ConclusionsTCRs targeting the KRASG12D peptide could be isolated from naive T cell repertoires. Integrating the TCR-peptide-HLA complex structure with a mammalian TCR library system could serve as a functional strategy to reduce potential TCR cross-reactivity with self-antigens, such as SMC1A29-38. Our findings evidenced an operable method to enhance TCR peptide specificity, while maintaining advanced functional avidity and potent anti-tumor activity.

MicroRNA Expression Profile in Acute Ischemic Stroke

Acute ischemic stroke with large vessel occlusion (LVO) continues to present a considerable challenge to global health, marked by substantial morbidity and mortality rates. Although definitive diagnostic markers exist in the form of neuroimaging, their expense, limited availability, and potential for diagnostic delay can often result in missed opportunities for life-saving interventions. Despite several past attempts, research efforts to date have been fraught with challenges likely due to multiple factors, such as the inclusion of diverse stroke types, variable onset intervals, differing pathobiologies, and a range of infarct sizes, all contributing to inconsistent circulating biomarker levels. In this context, microRNAs (miRNAs) have emerged as a promising biomarker, demonstrating potential as biomarkers across various diseases, including cancer, cardiovascular conditions, and neurological disorders. These circulating miRNAs embody a wide spectrum of pathophysiological processes, encompassing cell death, inflammation, angiogenesis, neuroprotection, brain plasticity, and blood–brain barrier integrity. This pilot study explores the utility of circulating exosome-enriched extracellular vesicle (EV) miRNAs as potential biomarkers for anterior circulation LVO (acLVO) stroke. In our longitudinal prospective cohort study, we collected data from acLVO stroke patients at four critical time intervals post-symptom onset: 0–6 h, 6–12 h, 12–24 h, and 5–7 days. For comparative analysis, healthy individuals were included as control subjects. In this study, extracellular vesicles (EVs) were isolated from the plasma of participants, and the miRNAs within these EVs were profiled utilizing the NanoString nCounter system. Complementing this, a scoping review was conducted to examine the roles of specific miRNAs such as miR-140-5p, miR-210-3p, and miR-7-5p in acute ischemic stroke (AIS). This review involved a targeted PubMed search to assess their influence on crucial pathophysiological pathways in AIS, and their potential applications in diagnosis, treatment, and prognosis. The review also included an assessment of additional miRNAs linked to stroke. Within the first 6 h of symptom onset, three specific miRNAs (miR-7-5p, miR-140-5p, and miR-210-3p) exhibited significant differential expression compared to other time points and healthy controls. These miRNAs have previously been associated with neuroprotection, cellular stress responses, and tissue damage, suggesting their potential as early markers of acute ischemic stroke. This study highlights the potential of circulating miRNAs as blood-based biomarkers for hyperacute acLVO ischemic stroke. However, further validation in a larger, risk-matched cohort is required. Additionally, investigations are needed to assess the prognostic relevance of these miRNAs by linking their expression profiles with radiological and functional outcomes.

Utilizing Simulated Telemedicine Encounters to Conduct Nationwide Studies of Physician Communication Practices.

Introduction: High-quality communication and shared decision making (SDM) are crucial elements of effective patient care. SDM conversations are intimate and logistically challenging to capture. Evaluating alternative methods to effectively observe how physicians conduct these conversations may improve research efforts in this core discipline of medicine. Methods: In two distinct qualitative studies (A and B), subspecialist physicians participated in a telemedicine encounter with a simulated patient facing a high-stakes treatment decision. Results: Thirty-seven transplant hematologists (A) and 21 medical oncologists (B) from across the United States successfully completed a telemedicine encounter with a trained actor, allowing for qualitative analysis of their SDM conversations. Discussion: Simulated telemedicine encounters are a feasible method for studying how a broad sample of subspecialty physicians conduct difficult conversations, such as emotionally fraught SDM conversations. This model could improve understanding of the current practice of SDM, identify opportunities for improvement, and serve as a framework for ongoing research of physician communication practices.

Enhanced Interleukin 6 Trans-Signaling Modulates Disease Process in Amyotrophic Lateral Sclerosis Mouse Models

Background/Objectives: Charcot first described ALS in 1869, but the specific mechanisms that mediate the disease pathology are still not clear. Intense research efforts have provided insight into unique neuroanatomical regions, specific neuronal populations and genetic associations for ALS and other neurodegenerative diseases; however, the experimental results also suggest a convergence of these events to common toxic pathways. We propose that common toxic pathways can be therapeutically targeted, and this intervention will be effective in slowing progression and improving patient quality of life. Here, we focus on understanding the role of IL6 trans-signaling in ALS disease processes. Methods: We leveraged unique mouse models of IL6 trans-signaling that we developed that recapitulate the production of active sIL6R in a genotypic and quantitative fashion observed in humans. Given that the SOD1 transgenic mouse is one of the most highly studied and characterized models of ALS, we bred SOD1G93A mice with IL6R trans-signaling mice to determine how enhanced trans-signaling influenced symptom onset and pathological processes, including neuromuscular junction (NMJ) denervation, glial activation and motoneuron (MN) survival. Results: The results indicate that in animals with enhanced trans-signaling, symptom onset and pathological processes were accelerated, suggesting a role in disease modification. Administration of an IL6R functional blocking antibody failed to alter accelerated symptom onset and disease progression. Conclusions: Future work to investigate the site-specific influence of enhanced IL6 trans-signaling and the tissue-specific bioavailability of potential therapeutics will be necessary to identify targets for precise therapeutic interventions that may limit disease progression in the 60% of ALS patients who inherit the common IL6R Asp358Ala variant.

Factors Affecting the Drought Preparedness in Somaliland

Somaliland, located in the Horn of Africa, has been increasingly vulnerable to recurring droughts over the past years, severely affecting its agricultural productivity, water resources, and overall resilience. Despite the rising frequency of these natural disasters, there is limited research on the factors influencing residents’ preparedness, which hinders effective disaster management efforts. The purpose of this study was to examine factors that influence the perceived effectiveness of Somaliland residents towards drought preparedness by utilizing an extended Protection Motivation Theory (PMT) approach. The latent variables that were considered in this study were Government Intervention, Drought Awareness, Environmental Awareness, Coping Appraisal, Threat Appraisal, Behavioral Intention, and Perceived Effectiveness. Two hundred fifty-four (254) people living in Somaliland from all regional areas willingly responded to the survey questions by utilizing a snowball sampling method. Partial Least Square-Structural Equation Modeling (PLS-SEM) showed that drought awareness and environmental awareness had significant effects on threat appraisal. In addition, environmental awareness also had a significant positive effect on coping appraisal. Finally, threat appraisal and coping appraisal had significant effects on behavioral intention, which subsequently led to perceived effectiveness. This study addresses a gap in academic research and disaster preparedness efforts in Somaliland by providing insights into the theoretical framework that can enhance the country’s preparedness for drought and other natural hazards.

Synthesis and analysis of an integrated suspension energy harvesting system for continuous half-width speed bumps

Global warming and climate change have become an important issue that the world is focusing on due to its apparent impact on lives and economies around the world. Efforts have been put into developing more environmentally friendly vehicles ranging from hybrid to fully electrical vehicles. In addition, research efforts have been made to develop and embed energy harvesters in vehicles to reuse some of the energy usually released into the environment. This study is about the development of an energy harvesting suspension system and investigates its performance using a continuous half-width deceleration bump. A 3D vehicle model with 4 harvesters embedded in the suspension is used in the analysis. The focus is on the effect of harvesters’ parameters and speed bump’s physical characteristics on the power generation performance of the harvesters. The main objective is to maximize electricity and minimize the ride comfort efficiency index using a multi-objective optimization function in conjunction with a genetic algorithm. This study proposes an innovative self-powering method that utilizes a chassis energy harvester to convert vibrations into magnetoelectric effects. It enables autonomous green energy generation for transportation vehicles and can be applied to vehicles traversing consecutive speed bumps. In addition to effectively reducing speed, the energy harvester generates green electricity to supplement the power supply of electric vehicles.

Global Educational Change under the Current Situation of Global Warming

Global warming has increasingly become the focus of global concern, which significantly impacts the ecological environment and social structure. The response to this crisis will require educational reform. This paper aims to green campus construction a crucial step in addressing the urgent environmental issues facing our planet. By embracing sustainable practices, schools can lead the way in promoting environmental awareness, cultivating green behavioral habits, and driving innovative solutions that contribute to the global community's overall health and well-being. The integration of environmental education and history discipline under the framework of multi-disciplinary integration education provides us with valuable experience and enlightenment. Interdisciplinary integration is an effective way to improve the quality of education and cultivate students' comprehensive literacy. By integrating knowledge, skills and values from different disciplines, the learning experience of children's green development concepts can be achieved through this process. As a large energy consumer, China has a significant impact on the global energy landscape. In the face of increasingly severe energy and environmental challenges, China has not only taken measures in terms of policy to adjust the energy structure, reduce the growth rate of energy consumption, and increase research efforts

Detection of Hymenoscyphus fraxineus in Leaf Rachises from European Ash (Fraxinus excelsior) in Germany

The ash dieback disease caused by Hymenoscyphus fraxineus is widespread in Germany and is the subject of intensive research efforts. The fungus identification is based on the genomic internal transcribed spacer (ITS) region, which can also be the site of intragenomic variability. In Germany, despite intense research efforts, only a few numbers of H. fraxineus sequence data are recorded. Therefore, this study aims to characterize H. fraxineus isolates obtained from diseased ash leaves in Brandenburg (Germany). Fungal isolates from infected ash leaf tissue were analyzed molecularly using species-specific primers and based on sequencing the ITS region of rDNA. The analysis of the two identified sequences revealed two base substitutionscompared to the reference sequences. Thus, they show an identity of 98.8%–100% to the reference sequences and support the assumption that H. fraxineus has multiple copies of the ITS region. The phylogenetic grouping with reference sequences did not show a distinct cluster for the European, particularly the German, sequences. This indicates that the evolvement of the genetic variability of the ITS region is still an ongoing process.

DECONSTRUCTED REALITY, TANGIBLE HARM

This paper studies the historical development of the international crime of incitement to genocide, from its origins in Nuremberg to its contemporary online format, identifying the role of the media and fake news in it. Showing this progression and describing its underlying patterns is a research effort for legally interpreting a phenomenon. After identifying the elements of the crime according to international law and presenting the new aspects brought by cybercommunications, the paper analyses the Myanmar case to demonstrate how the existing legal framework may be applied to social media posts spreading fake news. Lastly, concerning speeches falling outside the scope of the norm, the paper proposes a new crime prohibiting the conduct of systematically creating or distributing fake news online when such conduct constitutes computational propaganda with the intent to harm groups protected under the Genocide Convention and assesses the democratic justification of the proposal.

Larviculture of the freshwater prawn Macrobrachium spp. Spence Bate, 1868 (Decapoda, Palaemonidae): a comprehensive review

Abstract The freshwater prawns of the genus Macrobrachium Spence Bate, 1868, hold major potential for sustainable aquaculture intensification and diversification. Beyond the dominant giant river prawn M. rosenbergii (De Man, 1879), successful larviculture and domestication of additional species could boost production. However, inconsistent hatchery results have hampered the wider application and commercialization of cultivation protocols. This comprehensive review aims to analyse past larviculture research efforts to advance Macrobrachium farming practices. Several published studies documenting larviculture experiments on various Macrobrachium taxa are examined. Larval developmental patterns, optimal rearing salinity and temperature ranges, stocking densities, feeding regimes, and reported survival rates are compared within and among species. Significant inter- and intraspecific variability is apparent, highlighting the need for species-specific approaches. Past studies have applied generalized techniques derived from M. rosenbergii with varying success, further motivating the analytical review of findings. Key bottlenecks impeding consistent hatchery seed production are identified. In particular, knowledge gaps regarding larval nutritional needs, biofouling prevention, and weaning method optimization present ongoing challenges. By reconciling contradictory results and emphasizing biological nuances, this review distinguishes priorities for strengthening protocol development. Priorities include standardizing assessments, disseminating optimized techniques tailored to particular target species, and outlining future research directions. With reliable hatchery production, additional Macrobrachium species could be incorporated into polyculture or mono-crop systems, supporting livelihood diversification and sustainable intensification of aquaculture.

Tumor-infiltrating plasma cells are a prognostic factor in penile squamous cell carcinoma.

Penile cancer (PeCa) is a rare disease with poor prognosis in the metastatic stage. Neither effective adjuvant nor palliative therapeutic options are available. Research efforts in this field have so far failed to establish robust predictors of survival. To identify prognostic targets in PeCa, the current project focused on characterizing the tumor microenvironment (TME). A study cohort of 93 men with PeCa was used for the construction of a tissue microarray and immunohistochemical staining for CD3, CD4, CD8, CD20, CD56, CD138, FoxP3, and PD-L1. The quantity and spatial distribution of tumor-infiltrating immune cells were analyzed using digital image analysis. PD-L1 staining of tumor and immune cells was manually scored (combined positivity score (CPS)). T cells, T helper cells, cytotoxic T cells (CTLs), and regulatory T cells were detected in > 90% of PeCa and B cells in 88%, plasma cells in 85%, and NK cells in 23%. Approximately 50% of the PeCa samples were PD-L1 positive. In the univariate survival analysis, high PD-L1 CPS, plasma cells, CTLs, and B cells were significantly associated with favorable overall survival (OS), and the latter two with adverse recurrence-free survival. In multivariate analysis, plasma cells remained a significant factor for favorable OS (p = 0.04). In this study, the immune cells in the TME, especially plasma cells, were favorably associated with patient survival compared to other established prognostic factors in PeCa. Contemporarily, plasma cells have been discussed in the light of contributing to responses to modern immunotherapies. The results of this study support this notion.

Public awareness of telephone number for medical emergencies: a scoping review.

Prompt activation of emergency medical services (EMS) constitutes the fundamental component of bystander response to time-dependent health crises. A clear understanding of the public ability to access EMS may help to guide interventions aimed at enhancing community preparedness for emergencies. This review was conducted to summarise studies that examined public knowledge of emergency phone numbers. The scoping review encompassed articles published since 2004 that reported the proportion of subjects who knew emergency phone numbers. Data sources included PubMed, Google Scholar and references of included articles. Relevant data from eligible publications were extracted manually to an author-developed data-charting sheet and analysed descriptively. Forty-eight articles were analysed. Reported studies, mostly cross-sectional surveys, were conducted in 26 countries, including 16 high-income, nine middle-income and one low-income country. The percentage of subjects who knew emergency numbers varied from 0.0 to 97.8 (median [interquartile range]: 64.3 [32.8-80.0]). For developed countries, it was significantly higher than for developing nations (69.6 [54.1-84.2] and 34.6 [19.4-61.5], respectively; p=0.003). The studies were generally inconsistent regarding the association of subjects' socio-demographic factors with knowledge of emergency numbers, suggesting the existence of geography-specific patterns. Available studies observed low community knowledge of emergency numbers, especially in developing countries, and suggest that the problem has a global scale. Further research efforts are required to determine the best strategies for enhancing the public ability to access EMS.

Home Health Care Research for Children With Disability and Medical Complexity.

Pediatric home health care represents a vital system of care for children with disability and medical complexity, encompassing services provided by family caregivers and nonfamily home health care providers and the use of durable medical equipment and supplies. Home health care is medically necessary for the physiologic health of children with disability and medical complexity and for their participation and function within home, school, and community settings. While the study of pediatric home health care in the United States has increased in the last decade, its research remains primarily methodologically limited to observational studies. Dedicated funding and research efforts are needed to transform American home health care research to address multifaceted outcomes valued by families and providers as well as payers and government programs. In this paper, we review the recent literature in pediatric home health care and then propose an actional agenda that could address its missing evidence base. We posit that pediatricians should partner with family caregiving experts and patients to advance knowledge about child and family health outcomes, home health care use, new models of care, and optimal approaches to education and training while also considering meaningful approaches to address disparities. The creation of an American pediatric home health care data-sharing consortium, patient registry, and reproducible access and quality measures is also needed. Most importantly, efforts should center on patient- and family-centered health priorities, with the goal of ensuring equitable outcomes for every child and family.

What is informal workplace learning: a qualitative meta-synthesis

Purpose The purpose of this study was to provide a model for informal workplace learning. Despite the prevalence and recognized importance of informal learning in the workplace, scattered literature and different perspectives have caused a lack of shared understanding about its nature, and little research effort has been made in this direction so far. Design/methodology/approach This paper identified the most significant components of informal workplace learning using the qualitative meta-synthesis method. For this purpose, after searching sources from different databases and screening them, 17 sources were selected due to compatibility with the research criteria. The texts extracted from the sources were analyzed using a thematic analysis method and synthesized using an aggregation approach. Finally, a themes network was created as a model with 8 main components and 11 sub-components. Findings The results indicated that eight components were considered significant to informal workplace learning, including learning stimulus, awareness and intent to learn, action or experience, critical reflection on action, facilitators, collective learning, self-directed and context. Originality/value This meta-synthesis contributes to the coherence and integration of the informal workplace learning literature. The obtained conceptual model, in addition to the general components of informal workplace learning, provides an explanation of the characteristics of each and the relationships between its components to achieve a complete understanding of the nature of informal workplace learning. Since no study has been done so far to comprehensively identify the most significant components of informal workplace learning, it is not possible to refer to their results in comparison with the results of this study.

Algae extract-based nanoemulsions for photoprotection against UVB radiation: an electrical impedance spectroscopy study

Skin cancer is one of the most common types of cancer worldwide, with exposure to UVB radiation being a significant risk factor for its development. To prevent skin cancer, continuous research efforts have focused on finding suitable photoprotective ingredients from natural sources that are also environmentally friendly. This study aimed to develop oil-in-water photoprotective nanoemulsions containing marine macroalgae extract. A Box–Behnken experimental design was used to identify the most promising formulation composition, resulting in optimal physical properties. These properties, including droplet size, polydispersity index (PDI), and zeta potential, were evaluated using dynamic light scattering (DLS). To assess the photoprotection capacity of the formulations, electrical impedance spectroscopy (EIS) was employed to evaluate alterations in the electrical characteristics of excised pig skin membranes placed in Franz cells equipped with a 4-electrode set-up. The final composition of the nanoemulsion was caprylic/capric triglycerides 4%, Macrogolglycerol ricinoleate 30%, and algae extract 1%. The nanoemulsions had an average droplet size of 128.5 ± 8.6 nm, a PDI of 0.25 ± 0.06, and a zeta potential of 45.14 ± 0.02 mV. Compared to the control group, the photoprotective capacity of the oil-in-water nanoemulsions was statistically significant. Specifically, only a 15% reduction in the skin membrane electrical resistance following UVB exposure was observed when the formulation containing algae extract was used, whereas a 50% reduction was observed for the vehicle. In conclusion, this work demonstrates that the developed nanoemulsions based on natural ingredients show promising protective capacity against UVB exposure of the skin.