CO2 Efflux Research Articles

Phages Affect Soil Dissolved Organic Matter Mineralization by Shaping Bacterial Communities.

Viruses are considered to regulate bacterial communities and terrestrial nutrient cycling, yet their effects on bacterial metabolism and the mechanisms of carbon (C) dynamics during dissolved organic matter (DOM) mineralization remain unknown. Here, we added active and inactive bacteriophages (phages) to soil DOM with original bacterial communities and incubated them at 18 or 23 °C for 35 days. Phages initially (1-4 days) reduced CO2 efflux rate by 13-21% at 18 °C and 3-30% at 23 °C but significantly (p < 0.05) increased by 4-29% at 18 °C and 9-41% at 23 °C after 6 days, raising cumulative CO2 emissions by 14% at 18 °C and 21% at 23 °C. Phages decreased dominant bacterial taxa and increased bacterial community diversity (consistent with a "cull-the-winner" dynamic), thus altering the predicted microbiome functions. Specifically, phages enriched some taxa (such as Pseudomonas, Anaerocolumna, and Caulobacter) involved in degrading complex compounds and consequently promoted functions related to C cycling. Higher temperature facilitated phage-bacteria interactions, increased bacterial diversity, and enzyme activities, boosting DOM mineralization by 16%. Collectively, phages impact soil DOM mineralization by shifting microbial communities and functions, with moderate temperature changes modulating the magnitude of these processes but not qualitatively altering their behavior.

Root decomposition and nutrient dynamics in multifunctional forage‐biomass buffer‐strip systems

AbstractPerennial crops are thought as a solution for enhancing food security and providing ecosystem services under a changing climate, including forage‐biomass production, reduced erosion and nutrient leaching, and soil C accumulation. However, the drivers of root decomposition and C allocation in perennial multifunctional forage‐biomass buffer‐strips as affected by fertility management are poorly elucidated. Thus, study objectives were to assess root decomposition and soil CO2 efflux on switchgrass (Panicum virgatum L.), eastern gamagrass (Tripsacum dactyloides L.), silphium (Silphium integrifolium Michx.), and intermediate wheatgrass Kernza (Thinopyrum intermedium [Host] Barkworth &amp; D.R. Dewey) treated with poultry litter (PL) relative to the unfertilized control. Root mass loss was greatest for silphium, owing to low neutral and acid detergent fibers and lignin contents (6%) relative to the other species (16%–25%). Root‐C loss was the greatest for intermediate wheatgrass (IW), mostly driven by hemicellulose degradation. Low root mass, surface area, and volume likely enhanced root decomposition for silphium and IW. Root mass loss and C, N, and P mineralization for novel perennial buffer‐strip species were greater in PL plots. Soil organic C stocks were mostly similar across forage‐biomass species × amendment combinations. Silphium‐PL had 27%–50% greater SOC stocks after 4 years, owing to higher root sloughing and C inputs to soil. Root decomposition rates were primarily affected by root chemical composition and morphology, while soil CO2 efflux was driven by soil moisture and temperature. Greater root sloughing, C inputs, and nutrient cycling showcase the potential for C storage and multiple purposes of novel perennial buffer‐strips.

Soil CO2 efflux response to two decades of altered carbon inputs in a temperate coniferous forest

Global soils play a critical role in carbon (C) cycling and storage, and even minor disturbances to soil C flux can cause CO2 release to the atmosphere, exacerbating the greenhouse effect. This study investigates the long-term effects of forest detrital manipulation on soil CO2 efflux at a temperate forest site in Oregon’s western Cascade Mountains. We assessed the variation in seasonal and diurnal autotrophic and heterotrophic contributions to in situ soil CO2 efflux after 25 + years of detritus additions and removals and found slight increases in soil CO2 efflux rates concurrent with slight increases in soil C stocks, relative to C input rates, that may reflect underlying changes to C cycling in this system resulting from sustained detritus manipulation coupled with environmental change. Total CO2 efflux experienced increased contributions from functionally autotrophic root and rhizosphere respiration relative to the heterotrophic component. Seasonal and diurnal differences between soil respiration rates by treatment suggest a soil moisture buffering effect provided by the extra woody detritus that may support vegetative growth at times when seasonal drought would ordinarily slow plant and soil microbial metabolic activity. Overall, this research highlights the long-term effects of sustained litter additions and removals on soil CO2 efflux, which can help illuminate the response of C cycling in forests to current and future global change.

Vegetation and Precipitation Patterns Define Annual Dynamics of CO2 Efflux from Soil and Its Components

Respiration of soil heterotrophs—mainly of bacteria and fungi—is a substantial part of carbon balance in terrestrial ecosystems, which tie up organic matter decomposition with the rise of atmospheric CO2 concentration. Deep understanding and prediction of seasonal and interannual variation of heterotrophic and autotrophic components of CO2 efflux from soil is limited by the lack of long-term, full-year measurements. To better understand the impact of current climate changes on CO2 emissions from soils in the mixed forest and mowed grassland, we measured CO2 efflux every week for 2 years. Heterotrophic (SOM-derived + leaf litter) and root-associated (root with rhizosphere microorganisms) components were partitioned by the root exclusion method. The total CO2 efflux from soil was averaged 500 g C m−2 yr−1 in the forest and 650 g C m−2 yr−1 in the grassland, with shares of the no-growing cold season (Nov–Mar) of 22% and 14%, respectively. The heterotrophic component of CO2 efflux from the soil averaged 62% in the forest and 28% in the grassland, and it was generally stable across seasons. The redistribution of the annual precipitation amounts as well as their deficit (droughts) reduced soil respiration by 33–81% and heterotrophic respiration by 24–57% during dry periods. This effect was more pronounced in the grassland (with an average decline of 56% compared to 39% in the forest), which is related to lower soil moisture content in the grassland topsoil during dry periods.

Thermal Adaptation of Enzyme‐Mediated Processes Reduces Simulated Soil CO2 Fluxes Upon Soil Warming

AbstractUnderstanding factors influencing carbon effluxes from soils to the atmosphere is important in a world experiencing climatic change. Two important uncertainties related to soil organic carbon (SOC) stock responses to a changing climate are (a) whether soil microbial communities acclimate or adapt to changes in soil temperature and (b) how to represent this process in SOC models. To further explore these issues, we included thermal adaptation of enzyme‐mediated processes in a mechanistic SOC model (ReSOM) using the macromolecular rate theory. Thermal adaptation is defined here to encompass all potential responses of soil microbes and microbial communities following a change in temperature. To assess the effects of thermal adaptation of enzyme‐mediated processes on simulated SOC losses, ReSOM was applied to data collected from a 13‐year soil warming experiment. Results show that a model omitting thermal adaptation of enzyme‐mediated processes substantially overestimates observed CO2 effluxes during the initial years of soil warming. The bias against observed CO2 effluxes was lower for models including thermal adaptation of enzyme‐mediated processes. In addition, for a simulated linear 3°C soil warming over 100 years, models including thermal adaptation of enzyme‐mediated processes simulated SOC losses of a factor of three smaller than models omitting this process. As thermal adaptation of microbial community characteristics is generally not included in models simulating feedback between the soil, biosphere and atmosphere, we encourage future studies to assess the potential impact that microbial adaptation has on soil carbon – climate feedback representations in models.

Influence of Clear Felling on СО2 Emission from the Podzolic Soil Surface of the Coniferous-Deciduous Forest (Middle Taiga, Komi Republic)

The impact of industrial logging on the carbon cycle of boreal forests is characterized by significant uncertainties, which is largely due to the lack of information on carbon fluxes (in particular, soil respiration) in felling sites. The aim of study is to assess the effect of clear felling on CO2 emission from the soil surface of a coniferous-deciduous forest on a typical podzolic soil (Albic Retisol). The investigation was executed during the snowless periods (May-October) of 2020–2022 in a coniferous-deciduous forest and its felling site carried out in the winter of 2020. The carbon dioxide emission was measured by a LI COR 8100 gas analyzer. A brief description of the weather conditions during the years of research and the dynamics of soil temperature at a depth of 10 cm is given. A positive, statistically significant relationship between soil respiration and soil temperature at a depth of 10 cm (R2 = 0.17–0.75; p 0.001) was detected for the analyzed objects. The correlation with soil moisture was both positive and negative and statistically insignificant except data obtained in 2022 in the undisturbed control forest. The high values of CO2 flux during the snowless period were observed in July–August and was 3.90–5.62 gC/ m2/ day and 2.3–2.5 gC/m2/day in undisturbed forests and felled areas, respectively. In 2021, the peak of CO2 release shifted to June. Clear felling has a negative effect on the soil respiration of Albic Retisol that decreased by 1.2–1.9 times in the conditions of the middle taiga of the Komi Republic. The most (55–66%) of the C–CO2 efflux during the snowless period was emitted during the summertime, and the vegetation period (May–September) contribution was 84–88%. The obtained data will serve to determine the role of industrial logging in the carbon cycle of taiga forests.

Accessing Fungal Contributions to the Birch Effect: Real-Time Respiration from Pore-Scale Microfluidics.

Drying and rewetting of soil stimulates soil carbon emission. The Birch effect, driven by these cycles, leads to CO2 efflux, which can be monitored using real-time mass spectrometry (RTMS). Although soil fungi retain water during droughts, their contribution to CO2 release during drying-rewetting cycles remains unclear. In this study, we present the first demonstration of integrating micromodels with RTMS to monitor the Birch effect by simulating drought and rewetting. Micromodels were inoculated with axenic fungal culture and dried to assess moisture retention. After drying, RTMS quantified CO2 release upon rewetting with H218O mixtures. Our results showed that soil fungi released CO2 upon rehydration and immediately utilized the external water source at the pore scale by generating subsequent 46CO2. This work is the first to integrate RTMS with microsystems to investigate pore-scale biogeochemistry and the involvement of fungi in the Birch effect.

Moderate Drought Constrains Crop Growth Without Altering Soil Organic Carbon Dynamics in Perennial Cup‐Plant and Silage Maize

ABSTRACTSilage maize (Zea mays L.) intensification to maximise biomass production increases greenhouse gas emissions, accelerates climate change and intensifies the search for alternative bioenergy crops with high carbon (C) sequestration capacity. The perennial cup‐plant (Silphium perfoliatum L.) not only serves as a viable bioenergy source but may also be a promising soil C conservator. However, the dynamics of soil organic C (SOC) under the C3 cup‐plant, exposed to moderate drought conditions, that reduces growth rate without causing crop failure, compared with the drought‐tolerant C4 maize, remains unexplored. Here, we investigated in a lysimeter experiment the effects of moderate drought stress on crop growth and soil CO2 efflux under cup‐plant and silage maize compared with well‐watered conditions. Soil CO2 efflux along with root and shoot biomass, soil moisture and temperature as well as SOC and nitrogen (N) were measured over three consecutive years. Irrespective of the watering regime, cup‐plant induced a greater soil CO2 efflux (16% and 23% for 2020 and 2021, respectively), which was associated with higher root and shoot biomass compared with silage maize suggesting a substantial contribution of the roots to total soil CO2 efflux. In addition, soil CO2 efflux correlated negatively with soil dissolved N and positively with microbial C:N imbalance suggesting that low soil N availability influences soil CO2 efflux through processes related to N‐limitation such as N‐mining. Strikingly, moderate drought had no effect on soil CO2 efflux and C content and microbial biomass C, but increased dissolved organic C and microbial biomass N in both crops suggesting a complex interplay between C availability, N‐limitation and microbial adaptation under these conditions. Although cup‐plant increased soil CO2 efflux, the observed higher root and shoot biomass even under moderate drought conditions suggests a similar soil C management as silage maize; however, this still requires longer‐term investigation.

Seasonal pattern of diel variability of CO2 efflux from a large eutrophic lake

Lake is commonly acknowledged as a contributor to atmospheric CO2. Current manual sampling for estimations of CO2 emissions from lakes predominantly relies on daytime CO2 efflux (FCO2) assessments, which tends to overlook the diel variability of FCO2. This potentially introduces bias into CO2 emission estimates. The magnitude of diel FCO2 difference between seasons and the relative importance of underlying drivers in large eutrophic lakes remain inadequately explored. Here, we estimated FCO2 based on the water quality and meteorological data from Lake Taihu, a large eutrophic lake in China, with a temporal resolution of 4 h throughout the year 2021. The lake was a CO2 source with an efflux of 0.56 ± 0.66 mmol C/m2/h. We observed a 14.07 % increase in nocturnal FCO2 compared to daytime levels annually. During the non-growing season, nocturnal FCO2 exceeds daytime levels by 12.72 %, rising to 39.84 % in the algae-growing season (April to September). Piecewise structural equation models highlight gas transfer velocity as a key driver of diel FCO2 changes, with seasonal algal growth intensifying diel CO2 partial pressure variability by enhancing gross primary production, thereby amplifying diel FCO2 fluctuations. We suggest that ongoing lake eutrophication, driven by global climate change and human activities, may introduce additional uncertainties in lake CO2 emission estimates.

Evaluating the tradeoffs between water conservation, aesthetic value, evaporative cooling and CO2 emissions in St. augustinegrass and buffalograss

Urban lawns comprise a significant portion of urban greenery and provide several ecosystem services. Nevertheless, maintaining lawns comes with significant water costs in semi-arid inland southern California, as they require consistent irrigation to stay healthy and productive. The main objective of this study was to evaluate the effect of a wide range of irrigation rates and frequencies applied autonomously by a smart ET-based controller on the aesthetic value, cooling potential, and CO2 efflux of two warm-season turfgrass species. For three years, we studied the responses of Buffalograss and St. Augustinegrass to six irrigation rates and two irrigation frequencies in Riverside, California. Under historical average climate conditions, the minimum irrigation rate for Buffalograss was 93 % ETo, and for St. Augustinegrass, it was 74 % ETo. Under projected future climate conditions, the estimated minimum irrigation rate for Buffalograss did not change, but for St. Augustinegrass, it increased by 4 % and 7 % in 2100 under low emission (RCP 4.5) and high emission (RCP 8.5) scenarios, respectively. On average, canopy minus air temperature in Buffalograss was 6.2 ℃, and in St. Augustinegrass, it was 1.1 ℃. The average CO2 efflux in Buffalograss was 122.3 µg CO2-C m−2 s−1, and in St. Augustinegrass, it was 182.8 µg CO2-C m−2 s−1. Our results showed that turfgrass aesthetic values, cooling potential, and CO2 efflux diminished as the irrigation rate decreased, but at different rates for each turfgrass species.

The role of management practices on soil seed bank agrobiodiversity and agronomic sustainability in a horticultural cropping system

ABSTRACT Understanding how soil management practices influence the agrobiodiversity of cropping systems is crucial to promoting and maintaining agronomic sustainability in the long term. This study aimed to analyze seed bank diversity and to evaluate the effects of conventional (CONV) and alternative monoculture (ALTMO) and biannual rotation (BIROT) soil management practices in a horticultural cropping system. Soil cores were collected to identify seed bank composition and diversity indices were calculated. Additionally, soil parameters, CO2 efflux, temperature, and moisture were monitored. The results showed that management systems did not influence the composition of soil seed banks showing a prevalence of nitrophilous species in CONV management. Furthermore, the abundance and richness in the CONV and BIROT managements were high and low respectively due to the higher nitrogen rates in the soil. In contrast, ALTMO showed low abundance and a high number of species favoring higher competitiveness with positive effects on crop productivity. A positive correlation between CO2 soil efflux and temperature with species richness and Simpson’s diversity index was observed in all management systems while the soil moisture was negatively influenced. Finally, adopting alternative management strategies can preserve and enhance agrobiodiversity, and crop yields, and may contribute to developing more eco-friendly cropping systems.

How different soil surface treatments in urban areas affect soil pore structure and associated soil properties and processes

Soil water and temperature regimes in urban environments are greatly affected by different surface treatments, and these may lead even to changes in soil properties. The goal of this study was to find out how soil properties had changed after 8 years of soil surface modification. Five surface treatment scenarios were considered: bare soil (BS), bark chips (BC), concrete paving (CP), mown grass (MG), and unmown grass (UG). X-ray computed tomography and micromorphological analyses showed that character of pores in soils with grass (MG, UG), which were mainly influenced by organisms living in soils and roots, differed from pore character in soil covers BC or CP, which mostly were impacted by organisms living in soils. Both groups differed from BS, which was predominantly affected by the regular treatment consisting in weed removal and soil loosening. Soil under BC was more compact than for other treatments due to decomposition of the bark chips mulch and migration of mulch components. Organic matter content was greatest but its quality lowest in the BC soil, followed by UG, MG, CP, and BS. The highest aggregate stability assessed using the water-stable aggregates (WSA) index was found for UG, followed by MG, BC, CP, and BS. The greatest water retention ability was observed for BC followed by UG, MG, CP, and BS. The unsaturated hydraulic conductivities for pressure head of –2 cm measured for UG, MG, and BS were much higher than were those for CP and BC. Finally, the greatest net CO2 efflux was measured for BC and MG, followed by UG, CP, and BS. CO2 emission correlated negatively with soil physical quality expressed as slope of the soil water retention curve at its inflection point. In general, the best soil conditions were observed for UG. No treatment considerably aggravated soil condition.

Continuous Monitoring of Soil Respiration After a Prescribed Fire: Seasonal Variations in CO2 Efflux

Prescribed burns have recently become a widespread environmental management practice for biodiversity restoration to reduce fuel load, to provide forest fire suppression operational opportunities, to favor plant recruitment or to manage wild species. Prescribed fires were again applied in Doñana National Park (southern Spain) after decades of non-intervention regarding fire use. Here, we assessed their impacts on the soil CO2 effluxes over two years after burning to test the hypothesis that if the ecosystem is resilient, soil respiration will have a rapid recovery to the conditions previous to the fire. Using soil automated CO2 flux chambers to continuously measure respiration in burned and unburned sites, we showed that soil respiration varies among seasons but only showed significant differences between burned and unburned plots in the fall season one year after fire, which corresponded with the end of the dry season. Comparing soil respiration values from the burned plots in the three fall seasons studied, soil respiration increased significantly in the fall one year after fire, but decreased in the following fall to the values of the control plots. This study highlights the resilience of soil respiration after prescribed fire, showing the potential benefits of prescribed fire to reduce catastrophic wildfires, especially in protected areas subjected to non-intervention.

Differences in Soil CO2 Efflux and Microbial Community Composition among Slope Aspects in a Mountain Oak Forest

As one of the main terrain factors, the slope aspect generally shows remarkable effects on microclimate and vegetation distribution. The purpose of this study was to determine the potential effects of the slope aspect on soil respiration and the soil microbial community in a temperate mountain forest. A field investigation was carried out in a mountain oak forest located at different slope aspects (northeast, northwest, southeast, and southwest), and soil respiration was continuously measured for 12 months. The soil’s bacterial and fungal taxa were analyzed during the growing season. Our results showed that average soil respiration on the southwest and southeast slope aspects was 72.9% higher than the average on the northeast and northwest slope aspects. The coefficient of variation of soil respiration had the highest value on the northwest aspect (20.9%) and the lowest value on the southeast aspect (6.9%). The southeast slope had significantly higher soil respiration and temperature sensitivity compared to the other three slope aspects. The slope aspect substantially affected the soil’s bacterial and fungal r/K strategy, showing the lowest values on the northwest aspect and the highest values on the southeast aspect. Differences in bacterial r/K, the ratio of microbial biomass carbon (MBC) to soil organic carbon (SOC), and SOC among slope aspects contributed to a 43%, 38%, and 32% variation in soil respiration, respectively. The variation of soil temperature across slope aspects showed an indirect effect on soil respiration through changing bacterial r/K and MBC/SOC. Our findings highlight that terrain plays a critical role in regulating the spatial heterogeneity of soil respiration in mountain forests, which could be explained by the differences in SOC and microbial community composition across slope aspects.

Assessment of soil carbon dioxide efflux from contrasting land uses in a semi-arid savannah ecosystem, northeastern Ghana (West Africa)

Soil respiration (SR) emits a vast amount of atmospheric carbon dioxide (CO2) and contributes largely to the global greenhouse gas budget. The study assessed the dynamics of SR rates in the Vea catchment in northeastern Ghana, a sparsely gauged semi-arid savannah ecosystem characterized by distinct patterns of soil CO2 efflux. Through field measurements using soil chambers, the study quantified soil CO2 efflux rates in different land use types (woodland, cropland and grazeland), and assessed the influence of soil moisture, temperature, and soil organic carbon stocks on SR variability. The highest soil CO2 fluxes (12.97 ± 0.89 Mg CO2C ha−1 yr−1) were recorded in woodland, followed by grazeland (9.10 ± 0.42 Mg CO2C ha−1 yr−1) with cropland having the lowest rate (5.61 ± 0.29 Mg CO2C ha−1 yr−1). We recorded mean annual soil CO2 flux of 9.23 ± 0.53 Mg CO2C ha−1 yr−1 across the land use types and also observed significant seasonal and spatial variations in SR rates. The highest SR rate (220 mg CO2C m−2h−1) was recorded in the wet months (Jul-Sept and Mar-May) and the lowest rate (30 mg CO2C m−2h−1) in the dry months (Nov-Jan). For the wet season, the mean weekly soil CO2 fluxes ranged between 140 and 160 mg CO2C m−2 hr−1 as opposed to 60–75 mg CO2C m−2 hr−1 for the dry season. Seasonal and spatial variations in SR rates were largely driven by land use type, soil moisture and the interaction of soil temperature and moisture. The results underscore the importance of understanding the emission patterns from various land uses in West African savanna ecosystems to harnessing their potential for climate change mitigation.

Simple and Accurate Representation of Cumulative Nighttime Leaf Respiratory CO2 Efflux.

Leaf respiratory carbon loss decreases independent of temperature as the night progresses. Detailed nighttime measurements needed to quantify cumulative respiratory carbon loss at night are challenging under both lab and field conditions. We provide a simple yet accurate approach to represent variation in nighttime temperature-independent leaf respiratory CO2 efflux in environments with both stable and fluctuating temperatures, which requires no detailed measurements throughout the night. We demonstrate that the inter- and intraspecific variation in the cumulative leaf respiratory CO2 efflux at constant temperature, at any length of night, scales linearly with the inter- and intraspecific variation in initial measurement of leaf respiratory CO2 efflux at the same temperature at the beginning of the night. This approach informs large-scale predictions of cumulative leaf respiratory CO2 efflux, which is needed to understand plant carbon economy in global change studies as well as in global modeling and eddy covariance monitoring of the land-atmosphere exchange of CO2.

Response of Root Respiration to Warming and Nitrogen Addition Depends on Tree Species.

Roots contribute a large fraction of CO2 efflux from soils, yet the extent to which global change factors affect root-derived fluxes is poorly understood. We investigated how red maple (Acer rubrum) and red oak (Quercus rubra) root biomass and respiration respond to long-term (15 years) soil warming, nitrogen addition, or their combination in a temperate forest. We found that ecosystem root respiration was decreased by 40% under both single-factor treatments (nitrogen addition or warming) but not under their combination (heated × nitrogen). This response was driven by the reduction of mass-specific root respiration under warming and a reduction in maple root biomass in both single-factor treatments. Mass-specific root respiration rates for both species acclimated to soil warming, resulting in a 43% reduction, but were not affected by N addition or the combined heated × N treatment.Notably, the addition of nitrogen to warmed soils alleviated thermal acclimation and returned mass-specific respiration rates to control levels. Oak roots contributed disproportionately to ecosystem root respiration despite the decrease in respiration rates as their biomass was maintained or enhanced under warming and nitrogen addition. In contrast, maple root respiration rates were consistently higher than oak, and this difference became critical in the heated × nitrogen treatment, where maple root biomass increased, contributing significantly more CO2 relative to single-factor treatments. Our findings highlight the importance of accounting for the root component of respiration when assessing soil carbon loss in response to global change and demonstrate that combining warming and N addition produces effects that cannot be predicted by studying these factors in isolation.

Differential responses of soil CO2 dynamics along soil depth to rainfall patterns in the Chinese Loess Plateau

Soil surface carbon dioxide (CO2) efflux not only originates from topsoils, but also significantly involves contributions from deeper soil layers. Soil surface CO2 efflux significantly fluctuated with rainfall patterns in arid and semiarid regions. However, how soil CO2 dynamics respond at different soil depths to varying rainfall patterns remains largely unclear. To address this gap, we continuously monitored soil CO2 concentrations, temperature, and moisture content at 10 cm, 50 cm, and 100 cm depths in situ under cropland and orchards located in the semiarid Loess Plateau over a full year. Rainfall events were meticulously recorded, categorizing them into light (<10 mm), moderate (10 mm–40 mm), and heavy (>40 mm) to discern their impact on soil CO2 dynamics. Specifically, soil CO2 flux was not affected during light rainfall. Moderate and heavy rainfall decreased soil CO2 flux at 0–10 cm by an average of 70% and 83%, respectively. This decrease was associated with reduced gas diffusivity across rainfall patterns. For instance, heavy rainfall reduced gas diffusivity by an average of 83% and 53% at 10 cm and 50 cm soil depths, respectively. Furthermore, soil CO2 concentrations slightly dropped as soil temperature decreased at 10 cm depth during light rainfall. Soil CO2 concentrations at 10 cm and 50 cm depths initially decreased by up to 15% and subsequently increasing by up to 52% during moderate and heavy rainfall. This response was likely influenced by temperature reductions and subsequent rises in moisture content, with a hysteretic response of soil CO2 concentrations to temperature. The rapid increase in soil CO2 concentrations was mainly due to a substantial decrease in gas diffusivity. Notably, heavy rainfall induced a delayed increase in soil moisture content at 50 cm depth and a significant decrease in CO2 concentration by 16% at 100 cm depth. A substantial decrease in soil CO2 concentrations in deep soil layers was primarily related to decreased soil temperature. Additionally, the observed soil CO2 dynamics were partly attributed to biotic factors (microbial biomass carbon and root density) mainly on cropland, but mainly abiotic factors (soil organic carbon and bulk density) under orchards. Overall, these results suggest that reduced gas diffusivity triggered by increased soil moisture content in topsoils and weakened biological processes caused by decreased soil temperature in deep soils typically drive the differential responses of soil CO2 dynamics to rainfall patterns.

Assessing sediment CO2 effluxes in the coastal ecosystem of North Sumatra, Indonesia

Coastal wetlands including mangrove play a vital role in regulating the local and global carbon cycle. Coastal areas contribute greatly to the carbon exchange process due to the complex interactions that occur between the atmosphere, land, and oceans. One of the important components in coastal carbon dynamics is CO2 gas exchange between soil, water and the atmosphere. This study aims to assess CO2 efflux across various land covers (namely natural mangrove, restored mangrove, and converted mangroves to oil palm and aquaculture pond) in the coastal areas of North Sumatra Province, and analyze the effect of sea tides and ebbs on the rate of CO2 efflux and their connection with the number and area of macrozoobenthos burrows. We applied direct sampling by using the static closed chamber method attached to portable CO2 analyzer. The mean of CO2 efflux in natural mangrove forest land covers was 866±585 mgCO2/m2/h during low tide conditions and 1137±792 mgCO2/m2/h during high tide conditions, followed by oil palm plantations at 760.71±341 mgCO2/m2/h, restored mangroves during low tide of 575.24±326 mgCO2/m2/h and 597.11±180 mg CO2/m2/h during high tide conditions, and the lowest was recorded in ponds at 588.55±358 mgCO2/m2/h. Further, we observed that tidal conditions affect the magnitudes of CO2 efflux in natural and restored mangrove forests, and we did not observe similar pattern in oil palms and ponds since these land covers were not influenced by regular tidal input. We also observed that no significant relationship between the number and area of macrozoobenthos burrows and CO2 efflux. Our findings suggest that CO2 effluxes in coastal wetlands are highly dynamic and presumably driven by complex factors and therefore, understanding their magnitudes and drivers requires extensive measurement covering large spatial and temporal scales.

Earthworms in an enhanced weathering mesocosm experiment: Effects on soil carbon sequestration, base cation exchange and soil CO2 efflux

Despite its attractiveness for long-term carbon dioxide removal (CDR), quantifying weathering and CDR rates for enhanced weathering is a significant challenge. Moreover, the role of soil organisms, such as earthworms, in enhancing silicate weathering (both physically and chemically) has been suggested, but there is limited quantitative data on how biota, especially earthworms, contribute to inorganic carbon sequestration. To address these gaps, we conducted a mesocosm experiment with earthworms and basalt.Results indicate increases in clay and cation exchange, causing a weathering rate of over 10−12 mol total alkalinity m2 s−1, in range with other basalt experiments. Basalt amendment increased dissolved inorganic carbon export by only 4 g CO2 m−2. During the 4.5-month experiment, we observed neither a change in organic nor in inorganic carbon content.In soils without earthworms, basalt amendment reduced soil CO₂ efflux by approximately 0.2 kg CO₂ m2, suggesting considerable CDR. This decrease was about two times larger than calculated inorganic CDR equivalents, suggesting changes in soil organic matter dynamics.Interestingly, earthworms reversed the basalt-induced reduction in soil CO₂ efflux. This reversal was partly due to reduced export of dissolved inorganic carbon but mainly driven by increased organic matter decomposition. Our study highlights the importance of including organic carbon dynamics when evaluating the CDR potential of enhanced weathering.