Efficient Spread Research Articles

Multiomics Investigation Revealing the Characteristics of HIV-1-Infected Cells In Vivo

For eradication of HIV-1 infection, it is important to elucidate the detailed features and heterogeneity of HIV-1-infected cells invivo. To reveal multiple characteristics of HIV-1-producing cells invivo, we use a hematopoietic-stem-cell-transplanted humanized mouse model infected with GFP-encoding replication-competent HIV-1. We perform multiomics experiments using recently developed technology to identify the features of HIV-1-infected cells. Genome-wide HIV-1 integration-site analysis reveals that productive HIV-1 infection tends to occur in cells with viral integration into transcriptionally active genomic regions. Bulk transcriptome analysis reveals that a high level of viral mRNA is transcribed in HIV-1-infected cells. Moreover, single-cell transcriptome analysis shows the heterogeneity of HIV-1-infected cells, including CXCL13high cells and a subpopulation with low expression of interferon-stimulated genes, which can contribute to efficient viral spread invivo. Our findings describe multiple characteristics of HIV-1-producing cells invivo, which could provide clues for the development of an HIV-1 cure.

On the Suitability of Compressive Sampling for LoRa Signals Classification

Sensor networks require even more reliable and power saving communication systems, especially as regards to Internet of Things paradigm. In this scenario, LoRa technology, based on an efficient Chirp Spread Spectrum modulation, is well tailored, granting both long range distance and low energy consumption in the communication link. Even though it is a recent solution, several instrumentation manufacturers are providing test facilities and setups to carry out reliable measurements on LoRa signals in both laboratory experiments and on-field applications. Unfortunately, these solutions are usually given as upgrade of expensive instruments operating in time and/or frequency domain. To overcome this drawback, the authors propose hereinafter an innovative approach based on compressive sampling to suitably reduce hardware requirements and dependably reconstruct the signal of interest from a reduced number of samples acquired in time domain. To assess its reliability and the potential attractiveness, the proposed methodology has been exploited to extract characteristic parameters from actual LoRa signals by mean of an experimental set-up, which takes care of their generation and acquisition. The obtained results highlight the promising performance of the proposed approach.

Enhancing carrier transport and carrier capture with a good current spreading characteristic via graphene transparent conductive electrodes in InGaN/GaN multiple-quantum-well light emitting diodes

In this work, InGaN/GaN multiple-quantum-wells light-emitting diodes with and without graphene transparent conductive electrodes are studied with current-voltage, electroluminescence, and time-resolved electroluminescence (TREL) measurements. The results demonstrate that the applications of graphene electrodes on LED devices will spread injection carriers more uniformly into the active region and therefore result in a larger current density, broader luminescence area, and stronger EL intensity. In addition, the TREL data will be further analyzed by employing a 2-N theoretical model of carrier transport, capture, and escape processes. The combined experimental and theoretical results clearly indicate that those LEDs with graphene transparent conductive electrodes at p-junctions will have a shorter hole transport time along the lateral direction and thus a more efficient current spreading and a larger luminescence area. In addition, a shorter hole transport time will also expedite hole capture processes and result in a shorter capture time and better light emitting efficiency. Furthermore, as more carrier injected into the active regions of LEDs, thanks to graphene transparent conductive electrodes, excessive carriers need more time to proceed carrier recombination processes in QWs and result in a longer carrier recombination time. In short, the LED samples, with the help of graphene electrodes, are shown to have a better carrier transport efficiency, better carrier capture efficiency, and more electron-hole recombination. These research results provide important information for the carrier transport, carrier capture, and recombination processes in InGaN/GaN MQW LEDs with graphene transparent conductive electrodes.

Поширення вірусу звичайної мозаїки квасолі з насінням phaseolus vulgaris cv Червона Шапочка

Досліджено ефективність двох штамів р. Bacillus, B. subƟlis 16 and B. pumilus 11, проти хвороб ячменю ярого, що спричиняються грибом Bipolaris sorokiniana (Sacc.) Shoemaker. Встановлено, що антагоністична активність залежить як від стійкості сорту, так і від штаму бактерії. Суттєве зниження розвитку темно-бурої плямистості листків відмічали на найбільш сприйнятливому до хвороби сорті ячменю Нащадок у разі обробки рослин суспензією клітин штаму B. subƟlis 16. За внесення в ризосферу ячменю бактеріальних суспензій обох штамів відмічене суттєве зниження розвитку звичайної кореневої гнилі на сприйнятливому до хвороби сорті ячменю ярого Сяйво, що, проте, не супроводжувалося збільшенням маси проростків. Суттєвий приріст біомаси коренів і проростків при обробці бактеріальними інокулянтами відмічали на сортах із середньою стійкістю до хвороби: Себастьян і Антей.

Phase II trial of Voyager-V1 (vesicular stomatitis virus expressing human IFNβ and NIS, VV1), in combination with cemiplimab (C) in patients with NSCLC, melanoma, HCC or endometrial carcinoma.

TPS3161 Background: VV1 is an oncolytic vesicular stomatitis virus engineered to express human IFNβ to enhance cellular anti-tumor immune responses and tumor selectivity, and the human sodium iodide symporter (NIS) for virus tracking by SPECT imaging. Cancer cells are often hyporesponsive to IFNβ, enabling the efficient spread of VV1 and resulting in increased oncolysis. Differently from other oncolytic viruses, VV1 is suitable for both intra-tumoral (IT) and/or intra-venous (IV) administration. Despite considerable anti-tumor activity with checkpoint inhibitors (CPI) among some malignancies, long term survival and overall cures remain elusive. Prior Ph 1 studies have shown significant anti-tumor activity among several malignancies when VV1 was administered either as monotherapy or in combination with a CPI, despite progression on prior CPI monotherapy. Furthermore, pre- and post-treatment biopsy evaluations after VV1 treatment have demonstrated T cell infiltration and inflammation in both IT injected and non-injected lesions. Among IV treated patients (pts), IFNβ was detectable in the serum correlating with viral replication, making it an effective biomarker. C is a high-affinity potent human IgG4 anti-PD-1 monoclonal antibody. Though approved for use in cutaneous squamous cell carcinoma, C has shown anti-tumor activity, similar to other CPI, in several other indications. Therefore, VV1 and C could be an attractive combination for the immunotherapy for several solid tumors. This study represents the first clinical evaluation of VV1 in combination with C in pts with advanced solid tumors. Methods: The Ph 2 Simon 2 stage five-arm study of IV administration VV1 in combination with IV C will enroll pts with advanced NSCLC, HCC, melanoma & endometrial cancer. Enrolled pts with NSCLC & melanoma will be recent CPI-progressors, whereas enrolled HCC & endometrial cancer will be CPI-naïve. The study’s objectives include assessment of preliminary anti-tumor activity, safety, & immuno-regulatory activity of the combination. Pts will receive IV VV1 once on D1 and IV C once every 3 weeks until confirmed disease progression or intolerable toxicity. Pts enrolled in one melanoma cohort will also receive IT VV1 administered to palpable lesions. Response will be assessed every 9 weeks per RECIST v1.1. The null hypothesis of each cohort’s ORR will be tested versus a one-sided alternative yielding a Type I error rate of 5% and power of 80%. Cohorts will be expanded based on signal of activity. Clinical trial information: NCT .

Experimental Transmission Studies of SARS-CoV-2 in Fruit Bats, Ferrets, Pigs and Chickens

Background: A novel zoonotic SARS-related coronavirus emerged in China at the end of 2019. The novel SARS-CoV-2 became pandemic within weeks and the number of human infections and severe cases is increasing. The role of potential animal hosts is still understudied.Methods: We intranasally inoculated fruit bats (Rousettus aegyptiacus; n=9), ferrets (n=9), pigs (n=9) and chickens (n=17) with 105 TCID50 of a SARS-CoV-2 isolate per animal. Animals were monitored clinically and for virus shedding. Direct contact animals (n=3) were included. Animals were humanely sacrificed for virological and immune-pathohistological analysis at different time points.Findings: Under these settings, pigs and chickens were not susceptible to SARS-CoV-2. All swabs as well as organ samples and contact animals remained negative for viral RNA, and none of the animals seroconverted. Rousettus aegyptiacus fruit bats experienced a transient infection, with virus detectable by RT-qPCR, immunohistochemistry (IHC) and in situ hybridization (ISH) in the nasal cavity, associated with rhinitis. Viral RNA was also identified in the trachea, lung and lung associated lymphatic tissue. One of three contact bats became infected. More efficient virus replication but no clinical signs were observed in ferrets with transmission to all direct contact animals. Prominent viral RNA loads of up to 104 viral genome copies/ml were detected in the upper respiratory tract. Mild rhinitis was associated with viral antigen detection in the respiratory and olfactory epithelium. Both fruit bats and ferrets developed SARS-CoV-2 reactive antibodies reaching neutralizing titers of up to 1:1024.Interpretation: Pigs and chickens could not be infected intranasally by SARS-CoV-2, whereas fruit bats showed characteristics of a reservoir host. Virus replication in ferrets resembled a subclinical human infection with efficient spread. These animals might serve as a useful model for further studies e.g. testing vaccines or antivirals.Funding Statement: Intramural funding of the German Federal Ministry of Food and Agriculture provided to the Friedrich-Loeffler-Institut.Declaration of Interests: All authors declare no competing interest.Ethics Approval Statement: The animal experiments were evaluated and approved by the ethics committee of the State Office of Agriculture, Food safety, and Fishery in Mecklenburg – Western Pomerania (LALLF M-V: LVL MV/TSD/7221.3-2-010/18-12). All procedures were carried out in approved biosafety level 3 (BSL3) facilities.

Incidence and disease control of Zucchini yellow mosaic potyvirus

Zucchini yellow mosaic potyvirus (ZYMV) was first identified in northern Italy. It likes other species of the family Potyuiridue. ZYMV has been recorded in many countries since 1981. The efficient intercontinental spread of the virus can be explained by international trading of infected seeds. Since coat protein (CP) analysis has become a primary method for taxonomic assignment of potyviruses the aims were to characterize this genomic region of ZYMV originating from virus-infected cucurbitaceous seedlings. Virus infection in cucurbits is typically associated with mosaic symptoms on leaves and lumpy, distorted fruit. The range of symptoms produced by each virus can overlap and plants are commonly infected by more than one virus at once. The viruses are spread by many species of aphids moving through or within a crop. Control options include: destroying old cucurbit crops as soon as harvesting is completed destroying weeds and volunteer cucurbits, within and around crops as these harbor the viruses and/or the aphids separating new crops from maturing crops as these will have high levels of virus infection avoiding overlapping crops of cucurbits.

Recrudescence of COVID-19: A review of literature and Indian perspective

: The present article aims to describe the sudden emergence of 2019 novel Corona virus its incidence and spread among the neighbouring countries and preventive measures that the World Health Organization and The Central Government of India is taking to ensure early detection and management of cases. : The study is cumulative of the recent literature released by the Central Government of India DHR and ICMR, the Chinese scientists handling the samples and Interim measures on controlling the 2019n-Corona virus by World Health Organization and American National Institute of Health. Inclusion criteriaArticles that discussed corona virus history and epidemiology. Case reports and Interim guidelines by WHO. Articles related to treatment of SARS and MERS in the past 10 years. The study was conducted at GIMS-VRDL, Department of Microbiology, Gulbarga Institute of Medical Sciences, Kalaburagi, Karnataka and articles were selected using PRISMA 2009 checklist. Exclusion criteria: Articles with no proves. : In a short period of 58 days 2019n-CoV has affected more than 82138 people globally and caused 2801 casualties (2744 deaths in mainland China and 57 deaths in the neighbouring countries). The novel corona virus has challenged human technology and development with its undetected highly efficient spread and mutation rate. The role of WHO and ICMR in providing interim guidelines to health care professionals and Research Scientists in identification, isolation, specimen collection and management of affected cases is commendable. On 17 February UNICEF has appealed an estimate of 42.5 million USD to financially support the outbreak response. With growing numbers of cases the fight against the corona virus is still at stake, research needs to be conducted for better understanding different routes of spread including vertical transmission in humans and the detailed genomic sequence, for developing diagnostic kits and efficient anti corona virus drugs and vaccinations.

The 2b protein and C-terminal region of the 2a protein indispensably facilitate systemic movement of cucumber mosaic virus in radish with supplementary function by either the 3a or the coat protein

BackgroundIn Raphanus sativus (Japanese radish), strain D8 of cucumber mosaic virus (CMV-D8) establishes a systemic infection and induces mild mosaic on upper, non-inoculated leaves, whereas strain Y of CMV (CMV-Y) causes only a local infection in the inoculated leaves. Here, we further analyzed the specific viral factor(s) of CMV-D8 that is (are) indispensable for systemic infection in Japanese radish.MethodsTo identify which genomic RNA(s) is (are) involved in systemic infection in radish, we carried out a pseudorecombination analysis between CMV-D8 and CMV-Y. With recombination analyses between CMV-D8 and CMV-Y using mutant/recombinant RNA2s, chimeric and point-mutated RNA3s, we identified viral factors that are indispensable for systemic infection.ResultsViral RNA2 and RNA3 of CMV-D8 facilitated efficient virus spread into the upper, non-inoculated plant tissues of radish (cv. Tokinashi), but not those of CMV-Y. Recombinant RNA2s demonstrated that the 2b protein (2b) and the C-terminus of the 2a protein (2a) of CMV-D8 have a crucial role in systemic infection. In addition, we used chimeric and point-mutated RNA3s to that Pro17 and Pro129 in the coat protein (CP) of CMV-D8 are involved in efficient systemic infection and that Ser51 in the 3a protein (3a) of CMV-D8 has positive effects on systemic spread. The results suggested that these viral factors facilitate systemic infection of CMV-D8 in Japanese radish.ConclusionThe C-terminal region of 2a, the entire region of 2b, and supplementary function of either Ser51 in 3a or Pro17/Pro 129 in CP confer systemic infectivity on CMV-D8 in radish. These results further elucidate the complex interaction of viral proteins of CMV to complete systemic infection as a host-specific manner.

Coronavirus Disease 2019 and the Pediatric Gastroenterologist.

Coronavirus Disease 2019 and the Pediatric Gastroenterologist.

A Study on Production and Marketing of Rice Cultivation in Vizianagaram District of Andhra Pradesh, India

The production and marketing of rice cultivation have been studied in Vizianagaram district of Andhra Pradesh during 2018-19. Tools such as costs and returns, marketing margins, input use efficiency, marketing margins and price spread were used for the study. The results have shown that the Benefit-Cost ratiois 1.05. The input use efficiency has shown a negative significance for chemical fertilizers,pesticides and seed rate. The price spread analysis has shown that the producers receive 27% of the consumer price. Marketing margin for the adopted marketing channel was worked out by comparing the prices prevailing at each stage of marketing. Since used prices were related to a particular point of time and as small concurrent margins were also worked out.

Structure, Function, and Antigenicity of the SARS-CoV-2 Spike Glycoprotein

SummaryThe emergence of SARS-CoV-2 has resulted in >90,000 infections and >3,000 deaths. Coronavirus spike (S) glycoproteins promote entry into cells and are the main target of antibodies. We show that SARS-CoV-2 S uses ACE2 to enter cells and that the receptor-binding domains of SARS-CoV-2 S and SARS-CoV S bind with similar affinities to human ACE2, correlating with the efficient spread of SARS-CoV-2 among humans. We found that the SARS-CoV-2 S glycoprotein harbors a furin cleavage site at the boundary between the S1/S2 subunits, which is processed during biogenesis and sets this virus apart from SARS-CoV and SARS-related CoVs. We determined cryo-EM structures of the SARS-CoV-2 S ectodomain trimer, providing a blueprint for the design of vaccines and inhibitors of viral entry. Finally, we demonstrate that SARS-CoV S murine polyclonal antibodies potently inhibited SARS-CoV-2 S mediated entry into cells, indicating that cross-neutralizing antibodies targeting conserved S epitopes can be elicited upon vaccination.

Current Spreading in Back-Contacted GaInP/GaAs Light-Emitting Diodes

The recently proposed diffusion-driven charge transport (DDCT) method can allow a paradigm shift in the design of optoelectronic devices, by changing both the current injection principle and the device structure. The DDCT injection technique is based on the bipolar electron and hole diffusion currents that are used to electrically inject charge carriers into an active region (AR) located outside the p-n junction. In this article, we study an interdigitated back-contacted DDCT-light-emitting diode (LED) based on a GaInP/GaAs double heterojunction (DHJ) structure consisting of lateral heterojunctions (LHJs) located above a uniform AR. The structure uses single-sided electrical injection and is suitable for large-area applications and thin-film devices with near-surface ARs. Our analysis, based on charge transport simulations, suggests that the structure permits more efficient current spreading and lower surface recombination than conventional structures, leading to a very high internal quantum efficiency (IQE) and injection efficiency exceeding 99%. Particularly, we investigate the implications of using the new structure for improving the efficiency of LEDs, bringing them closer to the threshold of electroluminescent cooling (ELC). The results predict an above-unity internal power conversion efficiency for the DDCT-LEDs, substantially exceeding the efficiency of conventional reference devices, highlighting the new possibilities that DDCT devices offer especially for high-power ELC at room temperature.

Listeria monocytogenes Exploits Host Caveolin for Cell-to-Cell Spreading.

Listeria monocytogenes moves from one cell to another using actin-rich membrane protrusions that propel the bacterium toward neighboring cells. Despite cholesterol being required for this transfer process, the precise host internalization mechanism remains elusive. Here, we show that caveolin endocytosis is key to this event as bacterial cell-to-cell transfer is severely impaired when cells are depleted of caveolin-1. Only a subset of additional caveolar components (cavin-2 and EHD2) are present at sites of bacterial transfer, and although clathrin and the clathrin-associated proteins Eps15 and AP2 are absent from the bacterial invaginations, efficient L. monocytogenes spreading requires the clathrin-interacting protein epsin-1. We also directly demonstrated that isolated L. monocytogenes membrane protrusions can trigger the recruitment of caveolar proteins in a neighboring cell. The engulfment of these bacterial and cytoskeletal structures through a caveolin-based mechanism demonstrates that the classical nanometer-scale theoretical size limit for this internalization pathway is exceeded by these bacterial pathogens.IMPORTANCEListeria monocytogenes moves from one cell to another as it disseminates within tissues. This bacterial transfer process depends on the host actin cytoskeleton as the bacterium forms motile actin-rich membranous protrusions that propel the bacteria into neighboring cells, thus forming corresponding membrane invaginations. Here, we examine these membrane invaginations and demonstrate that caveolin-1-based endocytosis is crucial for efficient bacterial cell-to-cell spreading. We show that only a subset of caveolin-associated proteins (cavin-2 and EHD2) are involved in this process. Despite the absence of clathrin at the invaginations, the classical clathrin-associated protein epsin-1 is also required for efficient bacterial spreading. Using isolated L. monocytogenes protrusions added onto naive host cells, we demonstrate that actin-based propulsion is dispensable for caveolin-1 endocytosis as the presence of the protrusion/invagination interaction alone triggers caveolin-1 recruitment in the recipient cells. Finally, we provide a model of how this caveolin-1-based internalization event can exceed the theoretical size limit for this endocytic pathway.

Efficient spreading and controllable penetration of high-speed drops on superhydrophobic surface by vesicles

Water drops with a vesicle surfactant exhibit efficient spreading and controllable penetration after high speed impact on a superhydrophobic surface.

Interdigital Structure Enhanced the Current Spreading and Light Output Power of GaN-Based Light Emitting Diodes

In this manuscript, blue light emitting diodes (LEDs) with comb-shaped mesa and interdigital electrodes structure based on gallium nitride (GaN) are presented. This kind of LEDs have showed higher light output power and more efficient current spreading, comparing with a reference large-area LED. For the optimized LED structure, the light output power and external quantum efficiency, considering the actual emission area, reach to 600 mW and 73% at a forward current of 350 mA. The experimental results are consistent with the predictions of current expansion equations. The comb-shaped mesa structure decreases the total internal reflection (TIR) and the interdigital electrodes improve the current crowding effect, as validated from the electrical and optical tests. Therefore, the efficiency and reliability of the LED devices have been significantly enhanced by reducing the transverse current path. This work should be of guiding significance for structural design of large-area/high-power optoelectronic devices.

The efficient Lamarckian spread of life in the cosmos.

In this Chapter we discuss the various mechanisms that are available for the possible transfer of cosmic microbial living systems from one cosmic habitat to another. With the 100 or so habitable planets that are now known to exist in our galaxy alone transfers of cometary dust carrying life including fragments of icy planetoids/asteroids would be expected to occur on a routine basis. It is thus easy to view the galaxy as a single connected “biosphere” of which our planet Earth is a minor component. The Hoyle–Wickramasinghe Panspermia paradigm provides a cogent biological rationale for the actual widespread existence of Lamarckian modes of inheritance in terrestrial systems (which we review here). Thus the Panspermia paradigm provides the raison d'etre for Lamarckian Inheritance. Under a terrestrially confined neoDarwinian viewpoint such an association may have been thought spurious in the past. Our aim here is to outline the main evidence for rapid terrestrial-based Lamarckian-based evolutionary hypermutation processes dependent on reverse transcription-coupled mechanisms among others. Such rapid adaptation mechanisms would be consistent with the effective cosmic spread of living systems. For example, a viable, or cryo-preserved, living system traveling through space in a protective matrix will of necessity need to adapt rapidly and proliferate on landing in a new cosmic niche. Lamarckian mechanisms thus come to the fore and supersede the slow (blind and random) genetic processes expected under neoDarwinian Earth centred theories.

Factors Influencing Behavioural Intention to Use Mobile Banking in Champawat District of Uttrakhand

India has more mobile connections compared to banking accounts, therefore GoI in Economic Survey 2014-15, proposed JAM (Jan-Dhan Yojana, Aadhar Number and Mobile Number) trinity to use ICT for more efficient and effective spread of formal banking even to the hilly areas where brick and mortar banks are challenging to build and sustain. Also, financial inclusion cannot happen without economic activity, and Mahatma Gandhi National Rural Employment Act (MGNREGA) is a policy which significantly helped to enhance the economic activity of rural India. Because of penetration of mobile technology and involvement of the same in financial inclusion, this research will contribute to understanding the constructs of mobile banking adoption in hilly rural area of Champawat District, Uttrakhand w.r.t population registered with MGNREGA. The authors found research is scarce for mobile banking adoption in hilly rural areas of India. (Mehta et, al 2019) may be the only study for Champawat district of Uttrakhand, using technology acceptance model (TAM) and total interpretive structural modelling (TISM) to develop a model. This paper takes model from Mehta et al. 2019 and examines the relationship between the constructs using structured equation modelling (SEM).

Immunity against sexual stage Plasmodium falciparum and Plasmodium vivax parasites.

SummaryThe efficient spread of malaria from infected humans to mosquitoes is a major challenge for malaria elimination initiatives. Gametocytes are the only Plasmodium life stage infectious to mosquitoes. Here, we summarize evidence for naturally acquired anti‐gametocyte immunity and the current state of transmission blocking vaccines (TBV). Although gametocytes are intra‐erythrocytic when present in infected humans, developing Plasmodium falciparum gametocytes may express proteins on the surface of red blood cells that elicit immune responses in naturally exposed individuals. This immune response may reduce the burden of circulating gametocytes. For both P. falciparum and Plasmodium vivax, there is a solid evidence that antibodies against antigens present on the gametocyte surface, when co‐ingested with gametocytes, can influence transmission to mosquitoes. Transmission reducing immunity, reducing the burden of infection in mosquitoes, is a well‐acknowledged but poorly quantified phenomenon that forms the basis for the development of TBV. Transmission enhancing immunity, increasing the likelihood or intensity of transmission to mosquitoes, is more speculative in nature but is convincingly demonstrated for P. vivax. With the increased interest in malaria elimination, TBV and monoclonal antibodies have moved to the center stage of malaria vaccine development. Methodologies to prioritize and evaluate products are urgently needed.

Mobile Banking – An Answer to Financial Inclusion in Hilly Rural India.

Financial Inclusion is still a major challenge for the India, despite of being one of major agenda since 2004-2005, due to difficulty in reaching far flung areas of the country comprising 600,000 villages. This has huge implications on economic development of the country. Therefore there is regular thrust from Government of India (GoI) to bring everyone to the ambit of formal banking system through various schemes. Mahatma Gandhi National Rural Employment Act (MGNREGA) is one of the major GoI schemes that have contributed significantly to financial inclusion in India. India has more mobile connections compared to banking accounts, therefore GoI in Economic Survey 2014-15, proposed JAM (JanDhan Yojana, Aadhar Number and Mobile Number) trinity to use ICT for more efficient and effective spread of formal banking even to the hilly areas where brick and mortar banks are difficult to build and sustain. Hence to understand the constructs of mobile banking adoption for financial inclusion in Champawat district of Uttrakhand, India under MNREGA, this research discusses revised technology acceptance model (TAM) and carefully picked constructs from literature review which were weaved together by using Total Interpretive Structural Modeling (TISM) to form a conceptual model for Champawat District of Uttrakhand, India.