The purpose of this study was to investigate the extent of price transmission along the agri-food supply chain in selected regions in Ghana. The extent of transmission was used to assess the efficiency and effectiveness of the agricultural marketing systems in the area. Efficient marketing systems are expected to reduce the issue of regional price fluctuations, encourage fair income distribution throughout the supply chain, and improve food security. The preliminary time series stationarity/unit root and seasonality tests were performed using the Augmented Dickey-Fuller test (ADF)/Kwiatkowski, Phillips, Schmidt, and Shin (KPSS) and Canova-Hansen seasonal stability tests respectively. Following the detection of statistically significant threshold effects by the Supremum Likelihood Ratio (Sup-LR) tests, the extent of price transmission was investigated using two-region threshold vector error correction models. The initial tests found that the monthly wholesale, retail, and producer price series for maize, cowpea, and yam used in the study were first difference stationary rather than seasonally integrated. The findings revealed that price signals in the agri-food markets were not fully transmitted across the supply chain. Thus, the extent of price transmission was generally low, creating unexploited arbitrage opportunities along the chain. The study identified high transfer costs (proxied by the threshold parameter) and actor-specific market power (as revealed by the Granger causality test) as the primary obstacles to effective market functioning in the area. Minimizing transfer costs through infrastructural development such as improvements in road networks linking farming communities and markets, access to price information, and regular stakeholder engagements could help improve the functioning of the agri-food markets.