Efficient Culture Research Articles

Cell Cycle Dynamics in the Microalga Tisochrysis lutea: Influence of Light Duration and Drugs.

Our investigation into Tisochrysis lutea's cell cycle regulation involved natural and chemical synchronization methods to maximize their proportion at the division phase (G2/M). Hence, cultures were grown under different light/dark cycles (24:0, 12:12, and 8:16 h) to assess the impact of extended dark periods on cell division. Flow cytometry analyses of the cell cycle revealed that extending the dark phase resulted in a higher number of cells entering G2/M. However, this remained a minority within the overall culture (peaking at 19.36% ± 0.17 under an 8:16 h L/D cycle). To further enhance synchronization, chemical agents (nocodazole, hydroxyurea, and aphidicolin) were tested for their efficacy in blocking specific cell cycle stages. Only aphidicolin successfully induced significant G2/M accumulation (>90%). The commitment point for cell division was examined by exposing cultures to varying light durations (0 to 8 h) and measuring cell concentration and size distribution every 4 h. Our findings identified a critical minimum cell size ("sizer") of approximately 56.2 ± 0.6 µm3 and a required minimal light exposure ("timer") of 4 h to reliably trigger cell division. These findings highlight key conditions needed for optimal division of Tisochrysis lutea, offering more controlled and efficient cultivation strategies for future biotechnological applications.

Machine Learning-Based Morel Mushroom Environmental Monitoring System

As living standards improve, the demand for edible fungi increases, making their efficient cultivation crucial for food supply and agricultural productivity. Traditional manual monitoring has limited accuracy, but the introduction of IoT and embedded technology enhances the intelligence and precision of agricultural monitoring, saving both labor and resources. This study designs a machine learning-based environmental monitoring and prediction system for edible fungi, utilizing low-power ZigBee networks and Kalman filtering to improve data accuracy. The system ensures real-time data transmission via the MQTT protocol and enhances security and scalability through cloud storage. By comparing Transformer, LSTM, and LSTM-Attention models, it was found that the Transformer model performed best in predicting environmental parameters, enabling proactive regulation, boosting yield and quality, and promoting the development of intelligent agriculture.

Identification and Functional Analysis of Key Genes Regulating Organic Acid Metabolism in Jujube Fruit

Organic acids are crucial indicators of fruit flavor quality, but the metabolic characteristics and regulatory genes of organic acids during jujube fruit development remain largely unexplored. In this study, the cultivar ‘Heigeda’ with a high organic acid content was used as the experimental material. The organic acid content was quantified, and key candidate genes were identified through transcriptome analysis. The results indicated that malic acid and citric acid were the main organic acid content in jujube fruit and increased gradually with fruit development. Transcriptome analysis identified nine genes associated with malic acid and seven with citric acid, with four genes co-regulating malic acid and citric acid. Functional assays by transient overexpression and silencing of these four genes in the jujube fruits revealed that overexpression significantly upregulated the malic and citric acid content. However, only the silencing of aconitase1 (ZjACO1) and aconitase3 (ZjACO3) significantly downregulated the content of malic and citric acids. Therefore, aconitase1 (ZjACO1) and aconitase3 (ZjACO3) are considered the key genes that regulate the metabolism of citric acid and malic acid in jujube fruits. Our study can enrich the regulation mechanism of the organic acid metabolism of jujube fruit and provide theoretical support for the efficient cultivation of jujube fruit.

Navigating the microalgal maze: a comprehensive review of recent advances and future perspectives in biological networks.

PPI analysis deepens our knowledge in critical processes like carbon fixation and nutrient sensing. Moreover, signaling networks, including pathways like MAPK/ERK and TOR, provide valuable information in how microalgae respond to environmental changes and stress. Additionally, species-species interaction networks for microalgae provide a comprehensive understanding of how different species interact within their environments. This review examines recent advancements in the study of biological networks within microalgae, with a focus on the intricate interactions that define these organisms. It emphasizes how network biology, an interdisciplinary field, offers valuable insights into microalgae functions through various methodologies. Crucial approaches, such as protein-protein interaction (PPI) mapping utilizing yeast two-hybrid screening and mass spectrometry, are essential for comprehending cellular processes and optimizing functions, such as photosynthesis and fatty acid biosynthesis. The application of advanced computational methods and information mining has significantly improved PPI analysis, revealing networks involved in critical processes like carbon fixation and nutrient sensing. The review also encompasses transcriptional networks, which play a role in gene regulation and stress responses, as well as metabolic networks represented by genome-scale metabolic models (GEMs), which aid in strain optimization and the prediction of metabolic outcomes. Furthermore, signaling networks, including pathways like MAPK/ERK and TOR, are crucial for understanding how microalgae respond to environmental changes and stress. Additionally, species-species interaction networks for microalgae provide a comprehensive understanding of how different species interact within their environments. The integration of these network biology approaches has deepened our understanding of microalgal interactions, paving the way for more efficient cultivation and new industrial applications.

Phosphorus fertilization enhanced overwintering, root system and forage yield of late-seeded alfalfa in sodic soils

Sowing date and soil fertility are very important factors in the overwintering and production performance of alfalfa (Medicago sativa L.), yet there’s a knowledge gap in knowledge on how late-seeded alfalfa responds to phosphorus (P) fertilization. A field study was conducted in Inner Mongolia from 2020 to 2022 using a split-plot design. The main plots consisted of five sowing dates (31 July, 8, 16, and 24 August, and 1 September), while the subplots involved five P application rates (0, 40, 70, 100, and 130 kg P2O5 ha−1). Throughout the growing seasons, the overwintering rate, root traits, forage yield, and yield components were measured. The results revealed a consistent decrease in overwintering ability and productivity with the delayed sowing. This reduction in overwintering rate was mainly due to diminished root traits, while the decrease in forage yield was largely associated with a reduction in plants per square meter. However, P fertilizer application to late-seeded alfalfa demonstrated potential in enhancing the diameter of both the crown and taproot, thus strengthening the root system and improving the overwintering rate, the rate of increase ranges from 11.6 to 49%. This adjustment could also improve the shoots per square meter and mass per shoot, increasing by 9.4–31.3% and 15.0–27.1% respectively in 2 years, which can offset the decline in forage yield caused by late sowing and might even increase the forage yield. Regression and path analysis indicated that alfalfa forage yield is primarily affected by mass per shoot rather than shoots per square meter. This study recommended that the sowing of alfalfa in similar regions of Inner Mongolia should not be later than mid-August. Moreover, applying P fertilizer (P2O5) at 70.6–85.9 kg ha−1 can enhance the forage yield and persistence of late-seeded alfalfa. Therefore, appropriate late sowing combined with the application of P fertilizer can be used as an efficient cultivation strategy for alfalfa cultivation after a short-season crop harvest in arid and cold regions.

Enhancing Spinach (Amaranthus tricolor L.) Growth Using Maggot Fermentation-Derived Liquid Organic Fertilizer and AB Mix in Drip Fertigation Systems

Spinach (Amaranthus tricolor L.) is a highly nutritious leafy vegetable widely cultivated for its rich content of vitamins and minerals. To enhance spinach production sustainably, this study investigates the use of liquid organic fertilizer derived from maggot fermentation combined with AB mix nutrients, applied through a drip fertigation system. The study was conducted using three different concentrations: 2.5 ml/L, 5 ml/L, and 7.5 ml/L, with the goal of identifying optimal conditions for maximizing spinach growth. The research utilized a two-factorial, Completely Randomized Design (CRD) with five replications to evaluate the effects of these nutrient combinations on key growth parameters, including plant height, leaf number, leaf area and weight of plants. Results indicated that a concentration of 7.5 ml/L provided the most favorable growth outcomes, with a 46.5 cm increase in plant height and a biomass accumulation of 61.8 grams, representing significant improvements over control treatments. The macro-nutrient content of the maggot-derived POC met national agricultural standards, except for pH, which improved to 7.10 when combined with AB mix. These findings suggest that integrating maggot POC with AB mix offers a cost-effective and environmentally friendly alternative to conventional fertilizers, promoting sustainable agricultural practices. This study contributes to developing efficient cultivation methods for spinach, addressing increasing market demands, and reducing reliance on chemical fertilizers.

Nitrogen reduces calcium availability by promoting oxalate biosynthesis in apple leaves.

N and Ca are essential nutrients for apple growth and development. Studies have found that Ca content was not low under high N conditions but was poorly available. However, the underlying physiological mechanism through which N regulates Ca availability remains unclear. In this study, apple plants were supplied with N and Ca to analyse the content, in situ distribution, and forms of Ca using noninvasive micro-test technique, electron probe microanalysis, Fourier transform infrared spectroscopy, and transcriptome analysis. A potential interaction was observed between N and Ca in apple leaves. The application of high N and Ca concentration led to a CaOx content of 12.51g/kg, representing 93.54% of the total Ca in the apple leaves. Electron probe microanalysis revealed that Ca deposited in the phloem primarily existed as CaOx rhombus-shaped crystals. Additionally, high N positively regulated oxalate accumulation in the leaves, increasing it by 40.79 times compared with low N concentration. Specifically, N induced oxalate synthesis in apple leaves by upregulating the MdICL, MdOXAC, and MdMDH genes, while simultaneously inhibiting degradation through downregulation of the MdAAE3 gene. Transcriptome and correlation analyses further confirmed oxaloacetate as the precursor for the synthesis of CaOx crystals in the apple leaves, which were produced via the 'photosynthesis/glycolysis -oxaloacetate -oxalate -CaOx' pathway. WGCNA identified potential regulators of the CaOx biosynthesis pathway triggered by N. Overall, the results provide insights into the regulation of Ca availability by N in apple leaves and support the development of Ca efficient cultivation technique.

Spheroids and organoids: Their implications for oral and craniofacial tissue/organ regeneration

Spheroids are spherical aggregates of cells. Normally, most of adherent cells cannot survive in suspension; however, if they adhere to each other and grow to a certain size, they can survive without attaching to the dish surface. Studies have shown that spheroid formation induces dedifferentiation and improves plasticity, proliferative capability, and differentiation capability. In particular, spontaneous spheroids represent a selective and efficient cultivation technique for somatic stem cells. Organoids are considered mini-organs composed of multiple types of cells with extracellular matrices that are maintained in three-dimensional culture. Although their culture environment is similar to that of spheroids, organoids consist of differentiated cells with fundamental tissue/organ structures similar to those of native organs. Organoids have been used for drug development, disease models, and basic biological studies. Spheroid culture has been reported for various cell types in the oral and craniofacial regions, including salivary gland epithelial cells, periodontal ligament cells, dental pulp stem cells, and oral mucosa-derived cells. For broader clinical application, it is crucial to identify treatment targets that can leverage the superior stemness of spheroids. Organoids have been developed from various organs, including taste buds, oral mucosa, teeth, and salivary glands, for basic biological studies and also with the goal to replace damaged or defective organs. The development of novel immune-tolerant cell sources is the key to the widespread clinical application of organoids in regenerative medicine. Further efforts to understand the underlying basic mechanisms of spheroids and organoids will lead to the development of safe and efficient next-generation regenerative therapies.

Integrating the land–water–climate nexus approach with spatial-based sustainable agriculture production

CONTEXTA policy on agricultural land zoning, based on land suitability maps, has been introduced to optimize the use of resources for crop production in Thailand. However, the impact of activities occurring during cultivation in different land suitability classes has not been considered. A land–water–climate nexus index (LWCNI) has therefore been introduced as a holistic analysis that integrates three indicators––land-use intensity, water-use intensity, and climate change impact in terms of GHG emissions. This can guide resource allocation by highlighting areas that need improvement. OBJECTIVEWe aimed to assess the land–water–climate nexus in different land-suitability classes for sugarcane and cassava in Thailand in order to compare the nexus performances and improve the planning and management of resources for sustainable production. METHODSThe LWCNI was developed and assessed by combining it with geographic information system–based tools for evaluating the nexus performances of sugarcane and cassava cultivation as per the agricultural zoning policies. The scope of the work was “from cradle to farm-gate”, and the final products considered were sugarcane and fresh cassava. RESULTS AND CONCLUSIONSThe results revealed that sugarcane cultivation in moderate-suitability areas is the most effective, with the best LWCNI score being 0.87, suggesting efficient cultivation practices with a minimal use of land and water resources, and a minimal climate change impact. The three indicators of land-use intensity, water-use intensity, and GHG emissions for sugarcane were estimated at 0.010 ha/t, 108 m3/t, and 6.2 kg carbon dioxide equivalent (CO2e)/t, respectively. Cassava cultivation in the high-suitability area was the most efficient, with the best LWCNI score, at 0.95, and using less land, fewer water resources, and having less of a climate change impact. The land-use intensity, water-use intensity, and GHG emissions of cassava were estimated at 0.030 ha/t, 255 m3/t, and 35.7 kgCO2e/t, respectively. Growing crops in marginal and unsuitable areas presented a risk of a high climate change impact as well as competition for water resources due to limited local resources. We found that, even in the same land-suitability class, the amount of fertilizer used by the farmers varied depending on their knowledge, experience, and finances. Excessive fertilizer application increased GHG emissions, which in turn brought about a decrease in the LWCNI score, leading to a poorer nexus performance. SIGNIFICANCEThe findings emphasize the relationship between land and water use and climate change impact across varying spatial contexts. The LWCNI approach offers valuable insights for supporting agricultural zoning policy.

A Comparison between the Production of Edible Macroalgae Worldwide and in the Mediterranean Sea

Macroalgae are beneficial for consumers and producers due to their high productivity, their chemical composition and their efficient cultivation without additional feed. Asia dominates global macroalgae production, while European production is still marginal and mainly based on wild harvesting in the North Atlantic. The European Commission has launched initiatives to promote the sustainable production, safe consumption and innovative use of macroalgae products in European regional seas, including the Mediterranean Sea. A variety of coastal and seabed types and a wide range of environmental conditions make the Mediterranean a hotspot of marine biodiversity while providing good conditions for the cultivation of macroalgae. The aim of this paper is to compare the global macroalgae production and macroalgae production in the Mediterranean Sea, focussing on the cultivation of edible macroalgae. The paper also discusses the limitations and possibilities of macroalgae production in the region. Macroalgae production is one of the most promising sectors of the blue economy in the Mediterranean. The production of edible macroalgae suitable for human consumption has great potential, considering future population growth and related food security and health issues, as well as the additional ecosystem benefits of macroalgae production.

Effect of Salt-Induced Stress on the Calorific Value of Two Miscanthus sacchariflorus (Amur Silvergrass) Varieties

This study was designed to investigate the relationship between the caloric value and salt tolerance of two varieties of Miscanthus sacchariflorus (Amur silvergrass: M127 and M022). The salt tolerance capacity, photosynthetic characteristics, Na+ and K+ uptake by the roots and aboveground parts, and caloric value of different parts of the aboveground parts were obtained under hydroponic conditions. The results showed that M022 was more tolerant to salt stress than M127 and the former had a higher photosynthetic efficiency as well as a lower aboveground Na+ accumulation, K+ efflux, and larger K+/Na+ ratio. The calorific values of stems, spear leaves, aging leaves, and functional leaves of the two varieties showed a decreasing trend with increasing NaCl concentration. At 270 mM NaCl, the calorific values of the stems, aging leaves, functional leaves, and spear leaves was reduced by 18.10%, 46.73%, 26.11%, and 18.35% for M022 and 41.99%, 39.41%, 34.82%, and 45.09% for M127 compared to the controls, respectively. We observed that the aging leaves of M022 had a faster decline rate in calorific value than those of M127, indicating that the aging leaves of M022 preferentially isolated the harmful Na+ ion, reduced its accumulation in other parts, and increased the K+/Na+ ratio in the corresponding parts, thus inhibiting the decrease in calorific value. Following this result, it can be inferred that M022 inhibited the decline in calorific values during stress by efficiently compartmentalizing the distribution of Na+ and K+. Our results provide a theoretical basis and technical support for the efficient cultivation of salt-tolerant energy plants in saline–alkaline soil.

Soil and weed management effects on floral hemp (Cannabis sativa L.) production and cannabinoid content

AbstractMany floral hemp (Cannabis sativa L.) growers rely on tillage and black plastic mulch to prepare soil for transplants, warm the soil, and control weeds. However, plastic waste and its high cost to growers drive the need for research to assess alternative weed and soil management practices. Experiments were conducted at two Maine locations in 2021 and 2022 to compare cover crop soil and weed management systems on hemp production, cannabinoid concentrations, and yield. Experimental treatments included (1) tilled raised bed‐black plastic mulch, (2) biweekly cultivation, (3) no weed control, and (4) mowed no‐till mowed winter rye (Secale cereale L. Aroostook). Experimental sites received twice the 30‐year monthly average rainfall in July and September 2021 leading to poorer production versus typical 2022 long‐term average rainfall. Ice damage reduced winter rye biomass in 2022 at University of Maine Rogers Farm, leading to several significant year by location and year by weed management interactions. Hemp grown in the no‐till winter rye mulch developed more slowly, produced lower yields, and had higher weed densities than cultivated or black plastic mulch treatments. Plant size, yield, and cannabinoid content were generally equivalent where weeds were controlled. Raised bed‐black plastic soil and weed management were most effective under excessively wet conditions to reduce soil moisture and improve drainage. Cannabinoid concentrations did not increase under high weed pressure, but cannabinoid yield was influenced by flower production, which was significantly lower in the mowed rye mulch and control treatments. Blending raised bed production with efficient cultivation warrants research attention.

Deficit Irrigation-Based Improvement in Growth and Yield of Quinoa in the Northwestern Arid Region in China

Given the current global water scarcity issues, which particularly affect arid regions such as northwestern China, it is crucial to find crop planting patterns that result in efficient water resource utilization. Quinoa, as a drought-resistant and highly nutritious crop, has garnered significant attention from agricultural researchers in recent years. From 2019 to 2020, a series of experimental studies were conducted under non-mulching drip irrigation conditions to investigate the growth adaptability and the response to different irrigation levels of quinoa in an arid region in northwestern China. A comparative analysis of quinoa’s dry matter accumulation, yield, thousand-grain weight, harvest index, and water use efficiency under varying irrigation levels revealed that increasing irrigation significantly enhanced quinoa’s dry matter accumulation and yield. By optimizing the irrigation strategies, we found that the water-saving practice of initiating moderate irrigation in the sensitive water-demanding stages (flowering and fruiting) of quinoa increased the yield. The experiment results showed that, in 2020, the optimal irrigation amount was 3675 m3·ha−1 during a 14-day irrigation cycle, meeting quinoa’s growth requirements while improving water resource utilization efficiency. This study not only provides a scientific basis for the efficient cultivation of quinoa in the arid regions of northwestern China, but also offers new insights into and technical support for agricultural water resource management in the region, contributing to the sustainable development of agriculture in arid areas.

Effects of row ratio and plant spacing for enhancing hybrid rice seed production in the tropics

The seed yield production determines the success of the development of hybrid rice. Here, an effective and efficient cultivation technology for producing hybrid rice seeds is optimized through spacing and row ratio management. This study aimed to evaluate the effects of spacing and row ratio of restorer (R) : CMS (A) on F1 hybrid rice seed production. The experiment was conducted at the Indonesian Center of Rice Research's experimental field in the dry season of 2016, using parental lines HIPA18 hybrid rice, i.e., CMS: IR79156A line and Restorer: IR53942 line. The experiment was laid out in a strip-plot design replicated three times. Two factors were studied, i.e., plant spacing between CMS lines and row ratio R by A-lines. The results indicated that the highest HIPA18 seed production was obtained from 20 cm x 20 cm spacing of CMS with row ratio 2R:14A, i.e., 3.62 tons ha-1. Plant spacing and row ratio interactions were improved tillers number per plant of restorer and F1 seed yields. Keywords: A-lines; CMS; F1 hybrid rice; R-lines

Maximizing Agricultural Performance in Developing Countries: Implementing Efficient Cultivation Strategies for Short-Term Harvests to Boost Production Rates and Accelerate Supply Chain Efficiency

Improving agricultural productivity in developing countries is crucial for food security and economic prosperity. This research investigates the application of effective agricultural systems focused at short-term harvests in order to increase production rates and optimize supply chain efficiency. This study's focus on innovative farming techniques, technology adoption, and supply chain management improvements desires to assist policymakers, agricultural practitioners, and stakeholders with practical knowledge. This study focuses on multiple cultivation using the ground and the spaces accessible above it; therefore, the cost will remain constant or as low as possible. Cultivators will use minimal effort to bring out maximum harvest where they will use less labour as the ground is the same. This study will discuss on efficient cultivation strategies, Short term harvests and production rates, Optimizing supply chain efficiency, Innovative farming techniques and technology adoption, Supply chain management improvements and actionable insights for sustainable agricultural development. Through an emphasis on innovative farming methodologies, technological integration, and enhancements in supply chain management, this study endeavours to furnish actionable insights crucial for policymakers, agricultural practitioners, and stakeholders invested in the agricultural sector. The study emphasizes the vital role of customized changes in agricultural methods in addressing hurdles and maximizing on potential for sustainable agricultural growth. (Kouvelis et al., 2006) According to the findings of this study, the majority of cultivators work on the ground surface. They are spending money on fertilizer, water, and insecticides. One farmer can grow cauliflower, which will occupy the ground's surface. Malabar spinach will occupy the air area starting two feet above the ground. Again, a loft can be built five to six feet above ground to grow several sorts of beans. Other short-term crops such as chilies, spinach, lettuce, and radish can be grown in the gaps between the cultivation bays. In sum, all four layers of crops will need the same quantity of fertilizer, insecticides, and water. Thus, the cost will be almost the same, but the outcome will be four times higher.

Bacteria from nodules of Abrus mollis Hance: genetic diversity and screening of highly efficient growth-promoting strains.

Abrus mollis Hance. (AM) is an important species used in southern Chinese medicine. It is mainly found in Guangdong and Guangxi provinces in China, and it is effective in the treatment of hepatitis. Endophytic bacteria are known to affect the growth and quality of medicinal plants. However, there are limited reports describing endophytic bacteria related to AM. In the present study, Illumina-based 16S rRNA gene sequencing was used to investigate the endophytic bacterial communities of root nodules of AM at five sampling sites in Guangxi. In addition, 179 strains of endophytic bacteria were isolated and categorized into 13 haplotypes based on recA sequence analysis. The phylogeny of the 16S rRNA gene sequences revealed a predominance of nonrhizobial endophytes. Microbial diversity analysis showed that Proteobacteria was the dominant phylum in all samples, while Bradyrhizobium was the dominant genus in different samples. An efficient strain, Rhizobium tropici FM-19, was screened and obtained through greenhouse experiments. The AM plants inoculated with this strain showed the best growth performance and high nitrogen fixation and nodulation capacity. Notably, total phenols and total flavonoids, important active components in AM, increased by 30.9 and 42.7%, respectively, after inoculation with Rhizobium tropici FM-19. This study provides insights into the complex microbial diversity of AM nodules and provides strain information for the efficient cultivation of AM.

GIS-Based Tool for Pest Specific Area-Wide Planning of Crop Rotation Distance with Land Use Data.

Crop rotation is an important strategy for pest reduction. For mono-, or oligophagous pests that overwinter at a previously infested site, crop rotation means that the pests must find new host crop sites in the following year, and it is more efficient if a pest-specific distance is applied. Here, we report the development of a GIS-based tool for efficient cultivation planning using the example of the pest complex pea moth (Cydia nigricana) and grain and green peas (Pisum sativum). Monitoring data for four consecutive years (2016-2019) from 513 sites were used. Infestation of pea seeds and the distance to the previous year's pea sites were recorded. An adjustable Python script was developed by means of infestation-distance-correlation as a pest and crop-specific minimum migration distance (MD). The output of the tool is a risk map as decision support for cultivation planning. It shows different risk buffers with distances from 1261 m to 1825 m, depending on the cultivation type. The web tool is easily adjustable to other pests and crops anywhere in the world. The tool helps to prevent damages caused by agricultural, mono-, or oligophagous insect pests and consequently reduces pesticide applications for the benefit of the environment and biodiversity.

Abstract 7561: Standardized conditions for growth of lung cancer tumoroids cultured reproducibly in 3D-cultures

Abstract Background: Lung cancer is the most common cause of cancer-related death in Sweden with an estimated mortality of 3500 persons in 2021. Late diagnosis and heterogeneity of the disease lead to difficulty in predicting optimal treatment regime for each case. Further development of personalized therapy would be facilitated by reliable and efficient cultivation of cancer cell samples from patients in a clinical setting. A promising method is to grow the cells as 3D structures known as tumoroids, though the choice of extracellular matrix substrate or solid support most suitable for this purpose should be elucidated. Aim: To assess which extracellular matrix substrates or solid supports which enable lung cancer tumoroid generation reproducibly without affecting the status or detection of genetic alterations and protein expression, using lung cancer cell lines. Methods: Eight lung cancer cell lines, where of three adenocarcinoma and five squamous cell carcinomas, were included in this project. Each cell line was characterized with immunohistochemistry (IHC) for commonly expressed antigens in the two lung cancer types. Also, protein detection with Western Blots was performed. Cell lines found not expressing relevant antigens associated with the specific lung cancer variant were discarded. The remaining cell lines were cultivated in BIOFLOAT TC-Platte 96 well plate, Nunclon Sphera flasks, or GrowDex-T hydrogel, later transferred to a Corning Elplasia 6 well plate, and monitored for tumoroid generation. Results: The lung squamous cell carcinomas HCC-95 and HCC-1588 were both positive for CK5 and p40, while H1703 was negative for both markers. The adenocarcinoma H1975 was positive for TTF-1, also, unexpectedly, seen for NCI-H1703, earlier defined as squamous cell carcinoma. The tested cell lines were successfully cultured as tumoroids in the BIOFLOAT TC- 96 well plate, Nunclon Sphera flasks, and GrowDex-T hydrogel. The mutational status of the tumoroids as compared to the original tumors is currently under evaluation. Conclusion: We here show successful 3D culturing of cell lines with alternative substrates. Further fine-tuning of the chosen reproducible system's simplicity and cost is required before culturing tumoroids from lung cancer patients can be launched. Citation Format: Karina Malmros, Daniel Carlsén, Hans Brunnström. Standardized conditions for growth of lung cancer tumoroids cultured reproducibly in 3D-cultures [abstract]. In: Proceedings of the American Association for Cancer Research Annual Meeting 2024; Part 1 (Regular Abstracts); 2024 Apr 5-10; San Diego, CA. Philadelphia (PA): AACR; Cancer Res 2024;84(6_Suppl):Abstract nr 7561.

Alteration of growth, phenology, and yield of lily flowers through the synergetic effect of light spectra and endophytic bacterial priming

The production of ornamental flowers in controlled environment agriculture (CEA) has gained significant attention due to its potential for efficient cultivation. This study aimed to evaluate the influence of light quality (red, blue, red+blue, and white) after priming bulbs with Bacillus subtilis (Bs) on lily plant growth, phenology, yield, ornamental attributes, and pigmentation. Both light quality and Bs treatment significantly affected vegetative and generative organ development and overall plant growth. Bulbs primed with Bs and grown under blue light exhibited the earliest budding and flowering phases, along with an extended flowering period. The highest flower yield and productivity were achieved in Bs-primed bulbs under blue light. Regarding qualities, the best scores were obtained for Bs-primed bulbs grown under blue or red+blue light, compared to control plants grown under red light. The combined effects of Bs treatment and light quality also impacted photosynthetic pigment content in lily leaves. Throughout the growing season, the highest levels of photosynthetic pigments were detected under white light. Although the ratio between Chl a with other photosynthetic pigments were decreased during the flowering period, Bs treatment positively influenced those rations during the flowering phase when plants grown under monochromatic lights (red and blue). In summary, to achieve high-quality lily flowers in CEAs, the most promising approach involves utilizing a red+blue light spectrum in combination with Bs priming. This research highlights the importance of optimizing growing conditions and understanding the interplay between light quality and biological treatments for enhancing ornamental flower production in CEA systems.

Effects of different colors of plastic-film mulching on soil temperature, yield, and metabolites in Platostoma palustre

Platostoma palustre is an annual herb and an important medicinal and edible plant in southern China. Plastic-film mulching is an effective agronomic practice in the cultivation system of P. palustre, of which black-film mulching is the most common. However, fewer researches have been focused on the use of other colors of plastic films in P. palustre cultivation. In this study, different colors (white, black, red, and green) of plastic film were adopted, and the effects of different colors of plastic film mulching on the soil temperature, yield, and metabolites of P. palustre were investigated. The results showed that the fresh weight of a single plant of the green film treatment was significantly higher than that of the white film treatment (n = top 28). Based on the results of three temperature measurements, the soil temperature was almost the highest in the red film treatment and lowest in the white film treatment. The metabolomic analysis revealed that a total of 103 differential metabolites were identified. Among these, the gluconic acid, deoxyribose, and N-Acetylmannosamine in the red film treatment presented the highest abundance compared with the other treatments, meanwhile, the abundances of the five monosaccharides in the red film treatment were significantly higher than those of the green film treatment. Moreover, the sucrose, trehalose, and D-(+)-trehalose in the green film treatment exhibited the highest abundance, and the abundances of eight different amino acids in the red film treatment were almost the lowest while those in the black film treatment were almost the highest. Further analysis of the membership function values indicated that the black and red film treatments might be more suitable for the cultivation and quality production of P. palustre in comparison with the other two treatments. This study will provide a theoretical basis for improving the efficient cultivation technology of P. palustre and forming a theoretical system of P. palustre film mulching cultivation.