Efficient Control Method Research Articles

Innovative Approaches to Combat Duck Viral Hepatitis: Dual-Specific Anti-DHAV-1 and DHAV-3 Yolk Antibodies

Background and Objectives: Duck viral hepatitis (DVH), a highly contagious and acutely fatal avian disease, is characterized by convulsions, acute death, liver swelling, and hemorrhage, leading to substantial losses in the duck industry. However, there is no efficient prevention and control method for DHV infection. Duck hepatitis A virus (DHAV) is one of the primary pathogens responsible for DVH. Methods: In this study, we prepared a highly effective anti-DHAV IgY antibody by immunizing Hy-Line Brown laying hens at the peak of egg production. Results and Conclusions: The neutralization index of this antibody was found to be up to 38.90 (DHAV-1 QYD strain) and 141.25 (DHAV-3 GY strain) in vitro. The antibody also exhibited effective prophylactic effects in a model of hepatic inflammation following the viral challenge of ducklings, with a dose of 0.5 mL per duckling (containing 64 mg/mL of IgY) significantly reducing DHAV-related mortality by 66%, providing substantial protection against the infection. Furthermore, it effectively alleviated oxidative damage caused by DHAV in the ducklings. The results of this study indicate that IgY has the potential for treating DHAV infection; it also provides a new way for the treatment of poultry diseases with specific antibodies.

ESO-Based Non-Singular Terminal Filtered Integral Sliding Mode Backstepping Control for Unmanned Surface Vessels.

Aiming at the control challenges faced by unmanned surface vessels (USVs) in complex environments, such as nonlinearities, parameter uncertainties, and environmental perturbations, we propose a non-singular terminal integral sliding mode control strategy based on an extended state observer (ESO). The strategy first employs a third-order linear extended state observer to estimate the total disturbances of the USV system, encompassing both external disturbances and internal nonlinearities. Subsequently, a backstepping sliding mode controller based on the Lyapunov theory is designed to generate the steering torque control commands for the USV. To further enhance the tracking performance of the system, we introduce a non-singular terminal integral sliding mode surface with a double power convergence law and redesign the backstepping sliding mode controller for the USV heading control. Meanwhile, to circumvent the differential explosion issue in traditional backstepping control, we simplify the controller design by utilizing a second-order sliding mode filter to accurately estimate the differential signals of the virtual control quantities. Theoretical analysis and simulation results demonstrate that the proposed control algorithm improves the convergence speed, adaptive ability, and anti-interference ability in complex environments compared to traditional linear backstepping sliding mode control, thereby enhancing its engineering practicability. This research offers a more efficient and reliable method for precise heading control and path tracking of USVs in complex and dynamic environments.

Optimization and Practice of a High-Strength Acoustic Wave Indirect Penetration Enhancement Scheme for the Drilling of Structural Coal Seams

The structural coal seam drilling process often faces challenges such as shallow drilling depth, low hole formation rate, and the presence of blind areas in gas control. To address these issues, this study proposes a novel high-strength acoustic penetration approach and optimization design method under in situ conditions. Field tests were conducted at the Yunnan Bailongshan Coal Mine and Huainan Xieqiao Coal Mine to evaluate the effectiveness of this technique. The results demonstrate that the coal seam or its roof can act as an acoustic energy converter to generate high-intensity acoustic waves that penetrate the coal seam, and the field test results confirm the efficacy of this method in increasing gas extraction. This study proposes a novel ‘hole replaces seam’ technique, optimizing the extraction process and reducing the risk of explosions and providing a more efficient and safer method for gas control in structural coal seams. Accordingly, a new technical method for replacing the bottom (top) extraction lane is proposed.

Control Design of Fractional Multivariable Grey Model-Based Fast Terminal Attractor for High Efficiency Pure Sine Wave Inverters in Electric Vehicles

In this paper, a fast and efficient control method is proposed for a pure sine wave inverter used in an electric vehicle system, which can provide better performance under transient and steady-state conditions. The proposed control technique consists of a fast terminal attractor (FTA) and a fractional multivariable grey model (FMGM). The FTA with finite time convergence offers a faster convergence rate of the system state and a singularity-free solution. However, if the uncertain system boundaries are overestimated or underestimated, chatter/steady-state errors can occur during the FTA, which can lead to significant harmonic distortion at the output of the pure sine wave inverter. A computationally efficient FMGM is incorporated into the FTA to solve the chatter/steady-state error problem when an uncertain estimate of the system boundary cannot be satisfied. Simulation results show that the proposed control technique exhibits low total harmonic distortion. Experimental results of a prototype pure sine wave inverter are presented to support the results of the simulation and mathematical analysis. Since the proposed pure sine wave inverter outperforms the classical TA (terminal attractor)-controlled pure sine wave inverter in terms of convergence speed, computational efficiency, and harmonic distortion elimination, this paper will serve as a useful reference for electric vehicle systems.

Bacterial biofilm inactivation by plasma activated nanobubble water

This research developed novel plasma activated nanobubble water (PANBW) by integrating atmospheric cold plasma and nanobubble water (NBW) technologies. Mixing of plasma reactive species with NBW to generate the PANBW makes it an effective solution for microbial biofilm inactivation and water treatment, possibly by leveraging the benefits of both technologies. Selected properties of PANBW, including the concentrations of reactive oxygen and nitrogen species (RONS) were characterized, and the stability of RONS during storage for 7 days were evaluated. The combination of argon and air as feed gases was used to determine the influence of feed gas type on RONS production and the effect of the generated PANBW on biofilm reduction. The effectiveness of PANBW in inactivating mixed-species bacterial biofilms was assessed against NBW, plasma activated water (PAW), and their combinations. This comparison involved treating biofilms of Salmonella enterica Typhimurium ATCC 13311 and Aeromonas australiensis, that were grown on stainless steel coupons by these solutions. The PANBW treatment was most effective in the inactivation of the tested mixed species biofilms with a reduction of >2 log CFU/cm2 in the biofilm population. The confocal laser scanning microscopy analysis was consistent with the bacterial inactivation results. This study highlights the potential of atmospheric cold plasma when combined with nanobubble technology, as a novel and efficient method for biofilm control and food safety applications.

The Efficacy and Acceptability of Flash Glucose Monitoring in Pregnant Women with Gestational Diabetes Mellitus: A Systematic Review.

Background: Gestational diabetes mellitus (GDM) occurs in approximately 9% of pregnancies, and proper glycemic control is of utmost importance in the prevention of GDM-associated obstetric complications. Flash glucose monitoring (FGM), a subtype of continuous glucose monitoring (CGM), offers intermittent blood glucose scanning and is considered a propitious alternative to the standard method of self-monitoring of blood glucose (SMBG). Aim: The aim of this review was to systematically assess the efficacy and acceptability of FGM in in pregnancies complicated by GDM. Methods: A systematic literature search was performed in the PubMed, MEDLINE, Scopus, and Cochrane databases. The review was conducted following the PRISMA guidelines, and the study protocol has been registered in the PROSPERO database with the registration number CRD42024545874. Results: A total of 872 articles were initially identified, 141 publications underwent an in-depth full-text analysis, resulting in 133 studies being excluded from further assessment. Eventually, eight studies were included in the analysis. Conclusions: The analysis revealed that FGM is a safe and efficient method of glycemic control in GDM. The majority of the studies consider its accuracy comparable to SMBG. Furthermore, FGM is well accepted by patients with numerous advantages in user-friendliness over SMBG.

Fixed-time adaptive sliding mode-based trajectory tracking control for Wheeled Mobile Robot: Theoretical development and real-time implementation

Wheeled mobile robots (WMR) are common autonomous systems requiring efficient control methods. This work investigates fixed-time adaptive sliding mode control (FxT-ASMC) for the trajectory tracking task of a WMR subject to disturbances/uncertainties. The developed control strategy seeks to avoid the chattering problem, cope with disturbances when the upper limit is undefined, and enhance the convergence of the system states. Indeed, a new adaptive technique has been developed to effectively handle disturbances, even in the absence of prior knowledge regarding their upper bound. This technique consists in estimating the switching gain for each channel separately. On the other hand, an appropriate design of the reaching law ensures the fixed-time convergence of the sliding variables to zero. Additionally, sliding variables are designed in a way that guarantees the tracking errors will stabilize within a set amount of time. This kind of convergence was demonstrated using Lyapunov stability theory. In order to realize the trajectory tracking control structure, the FxT-ASMC system is incorporated with a classical kinematic controller. The efficiency of the suggested control methodology is proven via simulations and practical implementation.

Phytophthora heterospora is the causal agent of black rot disease on the orchid Cattleya leopoldii in Brazil.

Widely produced and marketed worldwide, orchids suffers from several diseases that have a negative effect on production. Black rot disease is among the most common and severe disease, characterized by black spots of rot on leaves, pseudobulbs and roots, which usually lead to the plant death. The world literature lists some Phytophthora species as causal agents of the disease, but there is no advanced study on the etiology of this disease in Brazil, which makes it difficult to determine an efficient control method. This work aims to contribute to the study of the etiology of black rot on Cattleya leopoldii in Brazil. Severely diseased C. leopoldii plants with typical symptoms of black rot, collected from a commercial orchid farm in Brazil, were taken to the laboratory and used to isolate the pathogen. Based on morphological characters analysis combined with molecular data, the isolates were identified as belonging to the species Phytophthora heterospora. This is the first worldwide report of P. heterospora causing black rot disease on orchids.

Efficacy of Pre and Post Emergence Herbicides against Complex Weed Flora, Yield and Economics of Blackgram under High Hills Wet Temperate Zone of Himachal Pradesh

Background: Implementing integrated strategies for weed management is essential in maximizing blackgram yield. Unchecked weed proliferation leads to substantial decrease in crop productivity. Methods: The current field experiment was conducted at Salooni, Himachal Pradesh, to evaluate the efficacy of different integrated weed management practices during Kharif 2022 and 2023. Eleven treatments targeting weed control were evaluated both at pre and post - emergence stages. Result: The predominant weeds were Echinochloa colona, Galinsoga parviflora, Cyperus species and other broad leaf weeds were Plantago lanceolata, Trifolium repens, Trianthema species, Ageratum conyzoides. Data on weed dynamics revealed that; after weed-free treatment, the application of Imazethapyr 75 g/ha as PE fb HW at 30 DAS; Pendimethalin 1.0 kg/ha as PE fb HW at 30 DAS and Pendimethalin 1.0 kg/ha + imazethapyr 75 g/ha (PE) recorded a significantly lower total weed count as compared to other treatments. Parameters related to blackgram growth and yield, including plant height, number of branches per plant, pods per plant, grain yield and haulm yield were significantly higher with weed-free treatment followed by application of imazethapyr 75 g/ha as PE fb HW at 30 DAS, remaining at par with application of pendimethalin 1.0 kg/ha + imazethapyr 75 g/ha (PE) and pendimethalin 1.0 kg/ha as PE fb HW at 30 DAS. The most economically efficient weed control method was the combination of pendimethalin 1.0 kg/ha + imazethapyr 75 g/ha (PE), as it resulted in the highest net returns and marginal benefit-cost ratio.

Application of Ethephon Manually or via Drone Enforces Bud Dormancy and Enhances Flowering Response to Chilling in Litchi (Litchi chinensis Sonn.)

Ethephon (2-chloroethylphosphonic acid) is frequently used for flush management in order to maximize flowering in litchi. However, the optimal dosage of ethephon, which balances between flush control effect and the detrimental effect on leaves, is unknown. This study aimed to identify the optimal ethephon dosage and test more efficient ethephon application methods, using a drone for flush control and flowering promotion in litchi. The effects of a single manual full-tree spray of 250, 500 or 1000 mg/L of ethephon in early November on the bud break rate, leaf drop rate, net photosynthetic rate, LcFT1 expression and floral induction (panicle emergence rate and panicle number) in ‘Jingganghongnuo’ litchi were examined in the season of 2021–2022. In the season of 2022–2023, the effects of drone application of 1000 mg/L of ethephon in early November on bud growth and floral induction were observed. The results showed that the manual ethephon treatments were effective at enforcing bud dormancy and elongating the dormancy period and that the effects were positively dependent on dosage. One manual spray of 1000 mg/L of ethephon in late autumn enabled a dormancy period of 6 weeks. The treatments advanced seasonal abscission of old leaves in winter and caused short-term suppression on photosynthesis within 2 weeks after treatment. Ethephon treatments, especially at 1000 mg/L, enhanced the expression of LcFT1 in the mature leaves and promoted floral induction reflected by earlier panicle emergence and increased panicle emergence rate and number in the terminal shoot. The floral promotion effect was also positively dosage dependent. The cumulative chilling hours below 15 °C from the date of treatment to the occurrence of a 20% panicle emergence rate were lowered in ethephon treatments. A drone spray of 1000 mg/L of ethephon solution consumed a sixth of the manual spray solution volume and was two thirds less effective in suppressing bud break compared with manual spraying. However, it achieved a significant flowering promotion effect comparable to traditional manual spraying. The results suggest that ethephon application enhanced flowering responsiveness to chilling as well as enforced bud dormancy. The application of ethephon with a drone proved to be an efficient method for flush control and flower promotion.

Differentiation of Vespa velutina nigrithorax Colonies Using Volatile Organic Compound Profiles of Hornets and Nests.

Vespa velutina (Lepeletier, 1836) (Hymenoptera: Vespidae) is a eusocial insect that lives in colonies of hundreds to thousands of individuals, which are divided into castes according to their task: queens, workers, and males. The proper functioning of the colony requires communication between the individuals that make up the colony. Chemical signals (pheromones) are the most common means of communication used by these insects to alarm and differentiate between individuals belonging or not to the colony. In this work, profiles of volatile organic compounds were obtained from the hornets and the external cover of four secondary nests located in the Basque Country. The obtained profiles were treated using chemometric tools. The grouping of hornets and nests according to the different colonies and geographical location was observed. In total, 37 compounds were found in common in hornets and nests. Most of them have been reported in the literature as belonging to different insects and plant species. This would corroborate the transfer of chemical compounds between the nest and the hornets' nest and vice versa. This information could be applied to the development of more efficient control methods for this invasive species, such as attractive traps or baits containing the relevant compounds.

Histological Investigations on Potato Tuber Moths, Phthorimaea operculella Treated with a Botanical Extract of Basil (Ocimum basilicum L), with Particular Attention to the Alterations in the Ultrastructure of the Larval Digestive Tract

Background: Phthorimaea operculella (Zeller), the potato tuber moth (PTM) is one of the most important pests that attacks potato, Solanum tuberosum L. crops worldwide. Adult moths attack tubers in fields as well as in storage. Infested tubers become unsuitable for trade or consumption, the tuber damage increases gradually after several generations of the pest during storage time. Botanicals have been found to be an efficient control method especially when they act as antifeedant or repellent, or even stomach poisons. The estimation of the mortal impact of some extracts against PTM was examined by emphasizing the Ultrastructure changes in the larval digestive tract by using scanning and transmission electron microscope. Results: Structural changes throughout the entire digestive tract (Fore-, Mid- and Hind-gut), induced by Basil extract were detected through both microscope, these changes leading lastly to the death of the treated larvae. Many different ruptures and malformations were detected throughout all the digestive tract assessment. Conclusions: Studies of the occurred changes revealed some histopathological changes through out all the digestive tract (Fore-, Mid- and Hind-gut), the attached muscular layer, tracheal system, and visceral fat bodies of the larvae when fed with tubers treated with Basil extract. From this findings, the use of plant extracts can be recommended for protection of potato tuber against the infestation by the tubers moths.

Highly Nonlinear Light-Nucleus Interaction.

The interaction between light and atomic nuclei is typically weak and confined within the linear and perturbative regime, which limits the achievable nuclear excitation probability and impedes potential applications such as nuclear optical clocks, nuclear lasers, and nuclear energy storage. In this Letter we show that the interaction between hydrogenlike thorium-229 ions (^{229}Th^{89+}) and contemporary intense lasers propels light-nucleus interaction into a highly nonlinear and nonperturbative regime. This interaction unlocks remarkably efficient nuclear excitation: over 10% of the ^{229}Th nuclei can be excited to the isomeric state by a single femtosecond laser pulse. Moreover, the laser-driven ^{229}Th^{89+} ions radiate multiple wavelengths of light that are high-order harmonics of the laser frequency, akin to the high harmonic generation process from laser-driven atoms but with distinct characteristics. These results pioneer a new frontier for exploring light-matter interaction, furnish a powerful method for efficient control over atomic nuclei, and pave a new way of nuclear coherent light emission.

Evaluation of Praziquantel effectiveness in treating Nile tilapia clinostomid infections and its relationships to fish health and water quality

This study aimed to conduct a multidisciplinary investigation integrating detailed morphology, molecular characterization, water parameters, histopathology alteration, and the trials of treatment of Clinostomum spp. In this study, 300 Nile tilapia (Oreochromis niloticus) were collected from the farmed and wild Nile River at Al Bahr Al Aazam, Giza Governorate to assess Clinostomid infection prevalence. Fish and water samples were collected from private fish farms, and water drains at Dakahlia, and Giza, Egypt. Analysis of the water revealed inadequate water quality, particularly in the fish farms. Snails and piscivorous birds were abundant at fish collection sites. The recovered Clinostomid MCs morphological characteristics and COI gene sequence analysis identified them as Clinostomum complanatum, C. phalacrocoracis, and Euclinostomum heterostomum. Clinostomid MCs disturbed the fish’s hematological and biochemical blood parameters. Bath treatment of parasitized fish with praziquantel (2 mg/L for 24 h) revealed a significant reduction in the number of vital MCs vs. infected fish (non-treated). Praziquantel (PZQ) is an effective and safe therapy for controlling Clinostomid infections affecting farmed Nile tilapia. The current findings indicate a link between poor environmental conditions and Clinostomum infections in tilapia. The study highlights the impacts of Clinostomid MCs on fish health and recommends bath treatment with PZQ as an efficient control method for these dangerous parasites to protect human and fish health.

Herbicidal weed management options for pigeon pea(Cajanus cajan) grown in southeastern Rajasthan

A field experiment was carried out during the kharif seasons of 2020–21, 2021–22 and 2022–23 at Kota, Rajasthan, to study the effect of post-emergence herbicides on productivity of pigeonpea [Cajanus cajan (L.) Millsp]. The results indicated that the minimum weed density and dry weight, and the highest yield attributes, yields, and net return of pigeonpea were obtained under weed free plot. All the herbicidal treatments significantly reduced the weed density and biomass compared to the weedy check. However, application of pendimethalin 30 EC @ 0.75 kg /ha (PE) fb Imazethapyr 10 SL @ 100 g/ha at 20–25 DAS + one inter-cultivation at 50 DAS recorded significantly higher plant height (1.82 m), pods/branch (22.96), pods/plant (206.3), weed control efficiency (84.39% at 60 DAS), grain yield (1,868 kg/ha), net return (` 87.66 x 103 /ha) and B: C ratio (3.35) over weedy check. This leads to the 61.73% higher pigeonpea grain yield over the weedy check plot. Hence it can be inferred that the ap- plication of pendimethalin 30 EC @ 0.75 kg /ha (PE) fb Imazethapyr 10 SL @ 100 g/ha at 20–25 DAS + one inter- cultivation at 50 DAS is an efficient weed control method for profitable cultivation of pigeonpea in south eastern Rajasthan.

Exploration of weak-PGML Method for Efficient Stability Control During Machining Operations

Stable control of the machining process can be difficult to maintain in production environments due to many factors, but especially uncertainty about selection of optimal machining parameters. The true underlying stability model for production machines on the shop floor is generally unknown, so parameters are typically selected by operators based on manufacturer recommendations or, in most cases, their accumulated shop floor experience. A class of methods referred to as physics-guided machine learning (PGML) offers a new approach for optimal parameter selection that incorporates theoretical knowledge about machining dynamics into the data-driven machine learning workflow. The focus of this paper is a version of PGML appropriate to shop floor operations in small and medium-sized machine shops (SMMs)—referred to as weak-PGML—that does not require a priori theoretical knowledge of machining dynamics to approximate the true underlying stability model for a particular machining scenario. Rather, the weak-PGML leverages the operator’s accumulated domain knowledge to uncover the true stability model and informs parameter selection. Weak-PGML reflects the limited resources and lack of technical expertise typical of most SMMs with legacy machines and a new generation of workforce without the accumulated experience of operators who have spent many years on the shop floor.

Characterization of Photobacterium damselae subsp. damselae isolated from diseased Asian seabass (Lates calcarifer) and the preliminary development of a formalin-killed cell vaccine.

Asian seabass (Lates calcarifer) is an economically important fish species that is widely cultivated in Thailand. However, aquaculture of Asian seabass is limited by infectious diseases. One of the most serious diseases is photobacteriosis, caused by Photobacterium damselae. Vaccination is recognized as an efficient disease prevention and pathogen control method for strengthening the aquaculture industry. To promote vaccine development, the characterization of pathogenic bacteria and their pathogenesis is required. In this study, isolates of P. damselae were obtained from commercial aquaculture farms in Thailand during 2019-2021. Analyses of 16S rRNA and the urease subunit alpha genes identified the isolates as P. damselae subsp. damselae (Phdd). Antibiotic susceptibility analyses showed that all Phdd isolates were resistant to amoxicillin (10 μg). Haemolysis and phospholipase activities were used to categorize P. damselae into three groups based on their biological activities. The pathogenicity of four candidates (SK136, PD001, PD002 and T11L) was tested in Asian seabass. Isolate SK136 showed the highest virulence, with a lethal dose (LD50) of 1.47 × 105 CFU/fish, whereas isolate PD001 did not show any virulence. Genotypic characterization, based on multi-locus sequence typing analysis, demonstrated that all candidates were novel strains with new sequence types (64, 65, 66 and 67). Preliminary vaccination using formalin-killed cells (FKCs) protected Asian seabass from artificial challenges. Taken together, these results provide fundamental knowledge for vaccine development against Phdd infection in Asian seabass.

Adaptive Current Angle Compensation Control Based on the Difference in Inductance for the Interior PMSM of Vehicles

Achieving good performance in terms of a fast and accurate maximum torque per ampere (MTPA) control method depends on both accurate parameter estimation and a sophisticated control strategy. However, it often requires a complex and long computational process. This paper proposes an efficient control method using the relationship of torque and current angle for maximum torque per ampere control of the interior permanent magnet synchronous motor (IPMSM) for vehicle application. It was found that it is not necessary for the control method to determine the inductance in the d-q axes; the identification of the difference between each axis is enough. Furthermore, this paper presents a simple and effective procedure to estimate the difference in inductance online, where the linear iron loss calculation method is also designed to support the above process. The proposed control method was experimentally validated on a 5 kW prototype by a TMS320F28335 microcontroller and DSPACE synchronized with a personal computer. The results show that the control process has faster and more accurate performance than the conventional method.

Efficient predictive control method for ORC waste heat recovery system based on recurrent neural network

The model predictive control performs well in regulating operating parameters and ensuring system safety when the organic Rankine cycles are operating with variable heat sources. However, traditional nonlinear predictive control based on the organic Rankine cycle mechanism model involves significant computational complexity, making it challenging to quickly find a control solution. This limitation hinders its application in the organic Rankine cycle for rapid response control. To address this issue, a fast model predictive control method is proposed in this work. A recurrent neural network model is well-trained using the input–output data of the organic Rankine cycle process, and it is used as a surrogate model in the design of the model predictive controller for the control of organic Rankine cycle operating parameters. The formulated optimal control problem is then transformed into a mixed integer linear programming problem, which can obtain high-quality and fast solutions during the control process. Through comparison with recurrent neural network-based nonlinear predictive control and pseudo-sequential method-based fast nonlinear predictive control, the results show that the designed controller can effectively accomplish the control of organic Rankine cycle operating parameters with smaller overshoot. Moreover, its average control solution time is shorter by 89.59% and 93.27% respectively while the total net output power of the system during the control process is 0.54% and 1.3% higher than that of the other two controllers. It exhibits superior control performance, even under variable waste heat conditions.

Improving Beef Cattle Production: Safety and Effectiveness of New Immunocastration Vaccine.

Reproductive control in mammals, particularly in beef production systems, is crucial for managing behaviors driven by sex steroids, which can cause biosecurity issues, reduced weight gain, and decreased meat quality. Additionally, controlling sexual activity in females prevents unwanted pregnancies when both sexes are housed together for fattening. Surgical castration in cattle, commonly performed under field conditions, is associated with significant welfare concerns due to pain and infection risks. Furthermore, castrating female cattle in the field is often impractically expensive. Hence, new reproductive control methods that prioritize animal welfare and are suitable for both sexes are essential. Immunocastration (IC), which involves vaccination against the GnRH-I hormone, has emerged as a promising alternative, demonstrating efficacy and safety in various species. Immunocastration has proven to be an effective alternative to surgical castration in controlling reproduction and promoting animal welfare in several species. This study aims to evaluate the safety, immunogenicity, immunocastration effect, and productive impact of a novel IC vaccine developed in Chile for female cattle. Two consecutive trials were conducted, the first under controlled conditions and the second under field conditions. The results demonstrated that the new vaccine is safe and effective for use in beef cattle, inducing specific immunity against GnRH-I, reducing gonadal functionality, and improving productive parameters. These findings suggest that this new IC vaccine can significantly benefit the beef cattle industry by providing a welfare-friendly and efficient method of reproductive control.