Efficient Access Research Articles

One-Pot Transition-Metal-Free Synthesis of Alkynyl Amides.

Alkynyl amides play crucial roles in organic synthesis in the production of bioactive compounds and valuable heterocycles. Despite numerous studies on their synthesis, challenges persist due to the necessity of harsh or hazardous conditions and the use of costly or unstable reagents. Herein, we present a one-pot method for the synthesis of all three bonds of the alkyne under transition-metal free conditions. An important feature of this chemistry is the use of readily available feedstock chemicals, such as methyl esters and acetamides. This approach offers efficient access to a wide range of aryl and alkyl alkynyl amides and demonstrates excellent tolerance towards various functional groups in a sustainable and cost-effective manner.

Nickel-Catalyzed Enantioselective Reductive N-Cyclization-Thiolation Reaction of Alkene-Tethered Oxime Esters and Disulfides.

Asymmetric aza-Heck cyclization and coupling reactions offer efficient access to enantioenriched N-heterocycles, yet the current studies focus primarily on sequential C-N and C-C bond formation. Herein, we report an enantioselective reductive aza-Heck cyclization followed by a C-S coupling sequence, ultimately yielding sulfide-containing enantioenriched pyrrolines. The reaction is conducted under mild conditions and tolerates broad functionalities including alkynes, phenols, anilines, amides, nitriles, and bromides.

Visible-light-induced dual C[sbnd]H functionalization of biaryl ynones via a 6-exo-trig domino radical addition/cyclization/dearomatization: Efficient access to alkylated spiro[5.5] trienones

A visible-light-induced 6-exo-trig tandem cyclization dearomatization of biaryl ynones with simple ethers was developed. The reaction involves selective C(sp3)H bond cleavage and dual CC bond formation providing efficient, atom and step economical access to alkylated spiro[5.5]trienones under mild conditions.

Flow Cytometry Sorting for Random Access in DNA Data Storage: Encapsulation for Enhanced Stability and Sequence Integrity of DNA.

As digital data undergo explosive growth, deoxyribonucleic acid (DNA) has emerged as a promising storage medium due to its high density, longevity, and ease of replication, offering vast potential in data storage solutions. This study focuses on the protection and retrieval of data during the DNA storage process, developing a technique that employs flow cytometry sorting (FCS) to segregate multicolored fluorescent DNA microparticles encoded with data and facilitating efficient random access. Moreover, the encapsulated fluorescent DNA microparticles, formed through layer-by-layer self-assembly, preserve structural and sequence integrity even under harsh conditions while also supporting a high-density DNA payload. Experimental results have shown that the encoded data can still be successfully recovered from encapsulated DNA microparticles following de-encapsulation. We also successfully demonstrated the automated encapsulation process of fluorescent DNA microparticles using a microfluidic chip. This research provides an innovative approach to the long-term stability and random readability of DNA data storage.

Abstract A028: Revisiting cancer care inequities in Sub-Saharan Africa: standpoint and recommendations

Abstract Introduction: Cancer is a major global public health concern that claims around 2000 lives per day in Africa alone. Mortality rates are expected to rise in the absence of appropriate interventions. Equity and prioritizing are the main obstacles to cancer care in Africa, as policymakers and other stakeholders in the healthcare system do not give cancer enough attention. All aspects of the cancer care continuum especially these three main levels of cancer care—prevention, diagnosis, and treatment/management—are negatively impacted by this neglect. Addressing concerns of equity and prioritizing is essential to promoting equality in cancer care, as it guarantees that all individuals have an equal opportunity at cancer prevention, early detection, appropriate therapy, and follow-up treatment. Methods: We present an overview of the current status of cancer care in Africa and offer recommendations to bridge the gap using the material currently accessible from recognized databases. Results: Insufficient funding for cancer research, inadequate screening or diagnostic facilities, a lack of a well-organized and efficient cancer registry system and access to care, a shortage of specialized medical staff, high costs for screening, vaccination, and treatment, a lack of technical capacity, poor vaccination response, and late patient presentation for cancer screening are some of the factors that we highlighted as contributing to the disparity in cancer care in Africa. We also offer suggestions on overcoming some of these barriers to achieving fairness in cancer care. Our recommendations are broken down into capacity-based and national-level initiatives. These include improving healthcare budgeting for personnel, supplies, and facilities; encouraging screening and vaccine uptake; raising awareness of cancer health among medical professionals during every hospital visit, gaining knowledge through specialized training, financing cancer research, offering cancer insurance, and putting mobile health technologies to use for telemedicine diagnosis.Conclusion: A comprehensive approach to addressing issues related to cancer equity would increase the chances of cancer patients surviving longer, reduce risk factors for at-risk groups, and guarantee fair access to cancer care across the continent. This report describes the present barriers to achieving equity in cancer care, identifies the African countries' current positions on the matter, and offers recommendations that could have the greatest impact on achieving equity in cancer care. Citation Format: John Oluwafemi Teibo, Olabode Omotoso, Festus Adebayo Atiba, Tolulope Oladimeji, Tomiwa Kehinde Paimo, Farid S. Ataya, Gaber El-Saber Batiha, Athanasios Alexiou. Revisiting cancer care inequities in Sub-Saharan Africa: standpoint and recommendations [abstract]. In: Proceedings of the 17th AACR Conference on the Science of Cancer Health Disparities in Racial/Ethnic Minorities and the Medically Underserved; 2024 Sep 21-24; Los Angeles, CA. Philadelphia (PA): AACR; Cancer Epidemiol Biomarkers Prev 2024;33(9 Suppl):Abstract nr A028.

Electrooxidative C(sp3)‒H Cyanation of Sparteine and Lupanine in Undivided Batch and Continuous‐Flow Cells

Sparteine is widely used as a chiral ligand in asymmetric synthesis, but methods for providing efficient access to functionalized sparteine derivatives are still limited. Herein, we describe an electrochemical α‐cyanation of sparteine‐type bis‐quinolizidine alkaloids. This method features commercially available setups for batch and single‐pass continuous flow conditions, enabling easy gram scale synthesis of valuable racemic and enantiopure products. Moreover, insights into the selectivity of the reaction and overoxidation mechanisms are disclosed. This allows for the development of divergent oxidation pathways depending on the electrolysis conditions.

Monitoring molecular events during photo-driven ubiquinone pool reduction in PufX+ and PufX− membranes from Rhobobacter capsulatus by time-resolved FTIR difference spectroscopy

The PufX protein is found in the photosynthetic membranes of several purple bacteria and is involved in ubiquinol-ubiquinone exchange at the QB site of the reaction center. We have studied quinone pool reduction in chromatophores from PufX+ and PufX− strains of Rhodobacter capsulatus by time-resolved FTIR difference spectroscopy under and after continuous illumination. To our knowledge, it is the first time that quinone pool reduction has been directly followed in real time in Rba. capsulatus membranes.Thanks to the availability in the literature of IR marker bands for protein conformational changes, ubiquinone consumption, ubiquinol production, Q---QH2 quinhydrone complex formation, as well as for RC-bound QA− and QB− semiquinone species, it is possible to follow all the molecular events associated with light-induced quinone pool reduction. In Rba. capsulatus PufX + chromatophores, these events resemble the ones found in Rba. sphaeroides wild-type membranes.In PufX− chromatophores the situation is different. Spectra recorded during 22.7 s of illumination showed a much smaller amount of photoreduced quinol, consistent with previous observations that PufX is required for efficient QH2/Q exchange at the QB site of the RC. Q consumption and QH2 formation are rapidly associated with QA− formation, showing that the structure of the RC-LH1 complex in PufX− membranes does not provide efficient access to the QB site of the RC to a large fraction of the quinone pool, evidently because the LH1 ring increases in size to impair access to the RC. The presence of a positive band at 1560 cm−1 suggests also the transient formation, in a fraction of chromatophores or of RC-LH1 complexes, of a Q---QH2 quinhydrone complex.Experiments carried out after 2-flash and 10-flash sequences make it possible to estimate that the size of the quinone pool with access to the QB site in PufX− membranes is ≥ 5 ubiquinone molecules per RC. The results are discussed in the framework of the current knowledge of protein organization and quinone pool reduction in bacterial photosynthetic membranes.

A Photocaged Microtubule‐Stabilising Epothilone Allows Spatiotemporal Control of Cytoskeletal Dynamics

AbstractThe cytoskeleton is essential for spatial and temporal organisation of a wide range of cellular and tissue‐level processes, such as proliferation, signalling, cargo transport, migration, morphogenesis, and neuronal development. Cytoskeleton research aims to study these processes by imaging, or by locally manipulating, the dynamics and organisation of cytoskeletal proteins with high spatiotemporal resolution: which matches the capabilities of optical methods. To date, no photoresponsive microtubule‐stabilising tool has united all the features needed for a practical high‐precision reagent: a low potency and biochemically stable non‐illuminated state; then an efficient, rapid, and clean photoresponse that generates a high potency illuminated state; plus good solubility at suitable working concentrations; and efficient synthetic access. We now present CouEpo, a photocaged epothilone microtubule‐stabilising reagent that combines these needs. Its potency increases approximately 100‐fold upon irradiation by violet/blue light to reach low‐nanomolar values, allowing efficient photocontrol of microtubule dynamics in live cells, and even the generation of cellular asymmetries in microtubule architecture and cell dynamics. CouEpo is thus a high‐performance tool compound that can support high‐precision research into many microtubule‐associated processes, from biophysics to transport, cell motility, and neuronal physiology.

A Photocaged Microtubule-Stabilising Epothilone Allows Spatiotemporal Control of Cytoskeletal Dynamics.

The cytoskeleton is essential for spatial and temporal organisation of a wide range of cellular and tissue-level processes, such as proliferation, signalling, cargo transport, migration, morphogenesis, and neuronal development. Cytoskeleton research aims to study these processes by imaging, or by locally manipulating, the dynamics and organisation of cytoskeletal proteins with high spatiotemporal resolution: which matches the capabilities of optical methods. To date, no photoresponsive microtubule-stabilising tool has united all the features needed for a practical high-precision reagent: a low potency and biochemically stable non-illuminated state; then an efficient, rapid, and clean photoresponse that generates a high potency illuminated state; plus good solubility at suitable working concentrations; and efficient synthetic access. We now present CouEpo, a photocaged epothilone microtubule-stabilising reagent that combines these needs. Its potency increases approximately 100-fold upon irradiation by violet/blue light to reach low-nanomolar values, allowing efficient photocontrol of microtubule dynamics in live cells, and even the generation of cellular asymmetries in microtubule architecture and cell dynamics. CouEpo is thus a high-performance tool compound that can support high-precision research into many microtubule-associated processes, from biophysics to transport, cell motility, and neuronal physiology.

Application of 5G network technology in agricultural production and information management

Establishing a management information system that adapts to the continuous development of agricultural production management today, adopting advanced 5G technology and means, and comprehensively managing the entire agricultural production is an inevitable trend in the development of agricultural production management. In order to improve the effect of agricultural production informatization management, this paper combines 5G (fifth-generation) network technology to construct agricultural production and informatization management models. In view of the massive dynamic agricultural production management data, this paper studies the efficient multi-copy management strategy, which can consider the two aspects of load balancing and energy efficiency at the same time, so as to achieve efficient data access and energy efficiency. Moreover, this paper introduces a time series-based file access heat calculation model. In terms of determining the number of copies of files, this paper proposes a copy factor allocation algorithm centered on the ranking of file access popularity, and gives a characteristic function. Through cluster analysis, it can be seen that the agricultural production informatization management model based on 5G technology proposed in this paper can effectively improve the efficiency of agricultural information management.

Access Characteristic-Guided Remote Swapping Across Mobile Devices

Memory swapping ensures smooth application switching for mobile systems by caching applications in the background. To further play the role of memory swapping, remote swapping across mobile devices has been widely studied, which caches applications to nearby remote devices by remote paging. However, due to the massive remote I/Os and unguaranteed swap throughput, the current remote swapping is limited with an unsatisfactory user experience, especially under variable network conditions. This paper first studies the access characteristics of applications and clarifies the impact of various network traffic on remote swapping. Motivated by these, an efficient access characteristic-guided remote swapping framework (ACR-Swap + ) is proposed to optimize remote swapping across mobile devices with resilient remote paging. ACR-Swap + first performs selective remote paging based on the swap-in frequency of different processes and then prefetches data across devices based on the process running states. Finally, it conducts hierarchical remote paging to avoid the impact of network traffic on remote swapping. Evaluations on Google Pixel 6 show that ACR-Swap + reduces the application switching latency by 21.6% and achieves a negligible performance fluctuation under various network traffic compared to the state-of-the-art.

Designing an Entrepreneurial Model of Remote Communication Technologies in Clinical Medicine: A Qualitative Study

Background: Today, information technology has permeated all fields, transforming numerous aspects of our lives, while entrepreneurship has become increasingly essential. In medicine, the integration of this technology is becoming crucial. Objectives: This research focuses on designing an entrepreneurial model that leverages modern communication and information technologies in the health sector. By capitalizing on created opportunities, entrepreneurs can drive innovation, leading to cost savings, time efficiency, and greater access to health and medical services for people everywhere. Methods: In this qualitative study, the sample included faculty members from Iran University of Medical Sciences, selected through purposive sampling. Following this, the sample was expanded using snowball sampling, introduced through key experts, to include additional informed individuals. Data collection was conducted through semi-structured interviews with faculty members, experts, and knowledgeable individuals who were willing to participate. The interviews continued until data saturation was reached. Data analysis was carried out using content analysis, with coding performed using MAXQDA10 software. Results: After coding and identifying the main and sub-themes, content analysis was completed, resulting in 10 main themes and 57 sub-themes. All interviewees highlighted sub-themes such as "infrastructure and hardware," "internet bandwidth," "access to electronic health records," "equipment and supplies," "data privacy," "qualified physicians," and "counseling." Conclusions: When designing an entrepreneurial model in any region or country, it is essential to consider the existing needs and conditions, environmental demands, population characteristics, culture, habits, preferences of service recipients, infrastructural capabilities, governing laws, and security issues. Additionally, the model must incorporate specific leadership, management, and oversight to ensure it becomes a responsive and effective system.

Correction: Zhu et al. Energy Efficient Access Point Placement for Distributed Massive MIMO. Network 2022, 2, 288–310

Following publication, concerns were raised regarding the peer-review process related to the publication of this article [...]

Enhancing Literature Review Efficiency: A Case Study on Using Fine-Tuned BERT for Classifying Focused Ultrasound-Related Articles

Over the past decade, focused ultrasound (FUS) has emerged as a promising therapeutic modality for various medical conditions. However, the exponential growth in the published literature on FUS therapies has made the literature review process increasingly time-consuming, inefficient, and error-prone. Machine learning approaches offer a promising solution to address these challenges. Therefore, the purpose of our study is to (1) explore and compare machine learning techniques for the text classification of scientific abstracts, and (2) integrate these machine learning techniques into the conventional literature review process. A classified dataset of 3588 scientific abstracts related and unrelated to FUS therapies sourced from the PubMed database was used to train various traditional machine learning and deep learning models. The fine-tuned Bio-ClinicalBERT (Bidirectional Encoder Representations from Transformers) model, which we named FusBERT, had comparatively optimal performance metrics with an accuracy of 0.91, a precision of 0.85, a recall of 0.99, and an F1 of 0.91. FusBERT was then successfully integrated into the literature review process. Ultimately, the integration of this model into the literature review pipeline will reduce the number of irrelevant manuscripts that the clinical team must screen, facilitating efficient access to emerging findings in the field.

Efficient access to non-damaging GaN (0001) by inductively coupled plasma etching and chemical–mechanical polishing

As third-generation semiconductors advance, gallium nitride (GaN)-based devices are gaining significant attention. However, the processing of GaN substrates directly affects device performance. This paper proposes an ICP etching-chemical–mechanical polishing (CMP) strategy to efficiently produce GaN single-crystal substrates with non-destructive, atomic-level surfaces. The process time is significantly reduced from 15 h to 1.5 h. The wet oxidation reaction during CMP eliminates impurities and defects from dry etching. FIB-STEM tests confirm that this method is non-destructive, as the ICP technique enables isotropic etching, and CMP introduces no new damage to the substrate. This approach enhances the productivity of large-size GaN single-crystal substrates, promoting their broader application in laser displays, RF devices, and power electronics.

Outcomes of the Health Data Research Network (HDRN) Canada and Canadian Longitudinal Study on Aging (CLSA) Partnership

Objective and ApproachThe Canadian Longitudinal Study on Aging (CLSA) is the largest national longitudinal cohort study, following ~50,000 adults across 10 provinces for at least 20 years. Health Data Research Network (HDRN) Canada is a distributed pan-Canadian network including members in all 13 provinces and territories. In February 2021, the organizations partnered with the objective to provide researchers with efficient access to multi-regional CLSA data linked to other health/health-related data through HDRN Canada’s data centres. A Steering Committee was convened including representatives from all parties to oversee the process from data sharing agreements (DSAs), negotiating terms of access, through to data transfer and linkage. ResultsIn Spring 2024, linkage was complete at four data centres (Ontario, New Brunswick, British Columbia, and Nova Scotia), with a DSA also in place for Newfoundland and Labrador. DSAs are nearing approval at three others (Manitoba, Prince Edward Island, and Quebec), and in progress in Alberta. Saskatchewan is working towards participation. ConclusionsThe HDRN Canada-CLSA partnership is on the way to making linked provincial CLSA cohort data available in all participating jurisdictions, facilitated by streamlined agreements and processes. ImplicationsCollaborative efforts of organizations such as HDRN Canada and CLSA to foster data linkages and streamline data access at a multi-regional level offers an unparalleled opportunity to explore the intersection between aging and health care utilization. Research enabled by this initiative will inform health care decision making and evidence-based policy development at multiple levels, from the individual to multiple levels of government.

Stereoselective Synthesis of Oxetanes Catalyzed by an Engineered Halohydrin Dehalogenase.

Although biocatalysis has garnered widespread attention in both industrial and academic realms, the enzymatic synthesis of chiral oxetanes remains an underdeveloped field. Halohydrin dehalogenases (HHDHs) are industrially relevant enzymes that have been engineered to accomplish the reversible transformation of epoxides. In our work, a biocatalytic platform was constructed for the stereoselective kinetic resolution of chiral oxetanes and formation of 1,3-disubstituted alcohols. HheC from Agrobacterium radiobacter AD1 was engineered to identify key variants capable of catalyzing the dehalogenation of γ-haloalcohols (via HheC M1-M3) and ring opening of oxetanes (via HheC M4-M5) to access both (R)- and (S)-configured products with high stereoselectivity and remarkable catalytic activity, yielding up to 49% with enantioselectivities exceeding 99% ee and E>200. The current strategy is broadly applicable as demonstrated by expansion of substrate scope to include up to 18 examples for dehalogenations and 16 examples for ring opening. Additionally, the functionalized products are versatile building blocks for pharmaceutical applications. To shed light on the molecular recognition mechanisms for the relevant variants, molecular dynamic (MD) simulations were performed. The current strategy expands the scope of HHDH-catalyzed chiral oxetane ring constructions, offering efficient access to both enantiomers of chiral oxetanes and 1,3-disubstituted alcohols.

Stereoselective Synthesis of Oxetanes Catalyzed by an Engineered Halohydrin Dehalogenase

Although biocatalysis has garnered widespread attention in both industrial and academic realms, the enzymatic synthesis of chiral oxetanes remains an underdeveloped field. Halohydrin dehalogenases (HHDHs) are industrially relevant enzymes that have been engineered to accomplish the reversible transformation of epoxides. In our work, a biocatalytic platform was constructed for the stereoselective kinetic resolution of chiral oxetanes and formation of 1,3‐disubstituted alcohols. HheC from Agrobacterium radiobacter AD1 was engineered to identify key variants capable of catalyzing the dehalogenation of γ‐haloalcohols (via HheC M1‐M3) and ring opening of oxetanes (via HheC M4‐M5) to access both (R)‐ and (S)‐configured products with high stereoselectivity and remarkable catalytic activity, yielding up to 49% with enantioselectivities exceeding 99% ee and E>200. The current strategy is broadly applicable as demonstrated by expansion of substrate scope to include up to 18 examples for dehalogenations and 16 examples for ring opening. Additionally, the functionalized products are versatile building blocks for pharmaceutical applications. To shed light on the molecular recognition mechanisms for the relevant variants, molecular dynamic (MD) simulations were performed. The current strategy expands the scope of HHDH‐catalyzed chiral oxetane ring constructions, offering efficient access to both enantiomers of chiral oxetanes and 1,3‐disubstituted alcohols.

Implementing geographic information systems in academic paper search: A framework and case study: GISAPS

The Geographic Information Systems Academic Publication Search (GISAPS) project pioneers the integration of spatial data into academic research through a novel online platform. Utilizing Web Map Service (WMS) and advanced search functionalities, GISAPS enables efficient access and analysis of georeferenced academic publications. This paper outlines the development and capabilities of GISAPS, including experimental WMS layer integration for enhanced data visualization. Key contributions include facilitating interdisciplinary research, improving spatial data accessibility, and promoting open science through its open-source framework. GISAPS's implications for academia and the research community are significant, offering new avenues for data-driven research and education. Future enhancements aim to incorporate real-time data feeds, advanced spatial analysis tools, and mobile accessibility, ensuring GISAPS remains at the forefront of GIS technology in academic settings.

The Use of Telehealth to Improve Office Efficiency and Health Care Access Among Patients Being Evaluated for Revision Total Joint Arthroplasty.

Patients being evaluated for revision total joint arthroplasty (RTJA) are often referred to tertiary care centers, which may decrease their access to adequate health care and overburden these health care systems. The purpose of this study was to evaluate the feasibility and effectiveness of RTJA patient evaluation via telehealth. We identified a consecutive series of patients newly evaluated for a symptomatic TJA by two academic surgeons during a 1-year period. Clinical records, radiographs, and laboratory values were reviewed to determine whether the patient was indicated for RTJA. Efficiency was determined by calculating the percentage of patients who could have been adequately evaluated with telehealth. We then used the modalities required for diagnosis in each RTJA case to determine the feasibility of evaluating such patients through telehealth. Of the 381 patients evaluated for RTJA candidacy, 154 (40.4%) were indicated for revision surgery. All 152 patients evaluated for possible hip revision could have been evaluated and diagnosed via telehealth, demonstrating a telehealth efficiency of 100%. Of 229 patients evaluated for possible knee revision, 183 were able to be evaluated and diagnosed via telehealth. The 46 remaining patients were indicated for revision secondary to instability, which would require an in-office examination for diagnosis. The efficiency of telehealth for potential knee revision patients was 79.9%. Telehealth may be useful in evaluating patients with symptomatic TJA. It may increase the efficiency of in-office evaluations and reduce potential barriers to health care access. [Orthopedics. 202x;4x(x):xx-xx.].