This paper presents the field tests and vibration performance assessment of two long-span floors with tuned mass dampers (TMDs). The floors considered are made of steel beams and concrete slabs, as part of a gymnasium with composite floors spanning 36 m in each direction and equipped with 30 TMDs. Operational modal analysis based on ambient acceleration measurements is performed to extract the modal parameters of the floors. Ambient vibration tests were conducted at three stages of construction for each floor, namely (i) after the concrete slab was completed, (ii) after one layer over the concrete slab was added, and (iii) after the flooring (surfacing) was fully finished. The effects of the layers making up the flooring system and of the TMDs on the dynamic properties of the floors are studied. The finite element models of the floors are validated using the identified modal parameters. The effects of natural frequency of TMDs on the dynamic properties of the floors are investigated using the validated model. Finally, the effects of flooring on the vibration serviceability of the two floors are studied with TMDs in operation, when the floors were subjected to crowd-induced rhythmic loading, from which the efficiency of TMDs is assessed numerically. The results show that the coupled vibrations of the two floors with TMDs turned off occur in the first two modes, while the natural frequencies of the floors decrease with the addition of layers. The TMDs in operation break the first mode of the floor into two modes with similar mode shapes, resulting in smaller vibration response and larger damping ratios, which vary with the natural frequency of TMDs. Also, the wood flooring significantly increases the human-induced vibration of the floor, while the plastic flooring shows basically no effect.