Precipitation Efficiency Research Articles

Improvement of microbial alkalinity resistance in self-healing cementitious materials by means of gradient domestication

The harsh environment of high alkalinity inhibits the effective induction of calcium carbonate precipitation by microorganisms within concrete. In this study, a gradient domestication method was employed to enhance the alkali tolerance of Sporosarcina pasteurii, and various activity parameters (OD600, conductivity, urease activity, and calcium carbonate content) were assessed in an alkaline cultivation setting. Subsequently, a comprehensive evaluation was conducted to compare the impact of domesticated microorganisms on the mechanical properties, impermeability, and self-healing capacity of concrete. Scanning electron microscopy (SEM) and X-ray diffraction (XRD) techniques were employed to characterize the morphology and composition of mineralization products. The results demonstrate that gradient domestication techniques effectively enhance the activity of microorganisms in highly alkaline environments and confirmed that enhanced alkalinity tolerance possesses the potential for multigenerational retention. In comparison to the undomesticated microbial samples at 28 d, concrete incorporating domesticated microorganisms exhibited a 16.59 % increase in compressive strength, a 37.74 % reduction in sorption, and a 23.08 % decrease in permeability. Additionally, the maximum closed crack width measured 0.57 mm, exceeding the 0.44 mm observed in the non-domesticated bacterial group. This study offers a promising strategy for improving the efficiency of microbial-induced calcium carbonate precipitation in concrete.

Improvement of the Sand Quality by Applying Microorganism-induced Calcium Carbonate Precipitation to Reduce Cement Usage

This research determines the Microbially Induced Calcium Carbonate Precipitation (MICP) process utilized by the bacteria found in Thailand. Many researchers typically use the high-efficiency MICP bacteria to precipitate calcium carbonate. However, it is only available in some countries, leading to a high import expense. Therefore, the methodology for using the bacteria capable of producing calcium carbonate in Thailand was investigated. The five pure bacteria strains are obtained from the Thailand Institute of Scientific and Technological Research (TISTR), i.e., Proteus mirabilis TISTR 100, Bacillus thuringiensis TISTR 126, Staphylococcus aureus TISTR 118, Bacillus sp. TISTR 658 and Bacillus megaterium TISTR 067. To screen urease production, the bacteria were spread on Christensen's Urea Agar (UA) slant surface via a colorimetric method. All bacteria strains can produce urease enzymes by observing the color changes in the UA. Berthelot's method was used to determine the urease activity. The result shows the bacteria's urease activity: 2389, 1989, 1589, 789, and 589 U/ml, respectively. These directly lead to calcium carbonate production: 3.430, 3.080, 2.590, 1.985, and 1.615 mg/ml, respectively. Despite the bacteria in this research having a low precipitation efficiency compared to the strain used in many research studies, they can improve sand stabilization in 7 days. Proteus mirabilis TISTR 100 was the most stable and effective strain for the MICP process in Thailand. Hence, this research reveals the ability of the local bacteria to bond with the sand particle. Briefly, the improvement of the MICP process in sand stabilization can be improved to reduce imported expenses. In addition, the MICP process can reduce the use of cement in sand stabilization work.

Removal of magnesium and aluminum from wet phosphoric acid by ralstonite precipitation: Thermodynamic and performance analysis, mechanistic studies and precipitate characterization

The purification of wet-process phosphoric acid has always posed a challenge for both academic and industrial researchers. In order to address this issue, a treatment unit was developed to mitigate magnesium and aluminum impurities from industrial semi-aqueous wet process phosphoric acid (SWPPA) (39.4 wt% P2O5) through ralstonite precipitation using Na+/F− as the precipitator (F− provided by HF). Thermodynamic analysis, DFT calculation and separation experiments results were used to investigate the impact of sodium sources properties on impurities removal rates and H3PO4 yield. Additionally, the effects of reaction temperature, reaction time, precipitating agent dosage, and aging time on precipitation efficiency were also examined. The results show that the ability of sodium source to ionize Na+ and react with H3PO4 and HF plays a crucial role in determining the impurity removal performance of magnesium and aluminum. The precipitants NaCl and Na2CO3 exhibit the highest removal rates for magnesium and aluminum, which is attributed to the easy ionization of Na+ ions. In comparison to Na2CO3, the higher H3PO4 yield with NaCl as precipitator can be achieved due to its lower nucleation rate and higher crystal growth rate in the phosphoric acid system. NaCl and HF as precipitants effectively reduces the concentrations of magnesium and aluminum in the untreated SWPPA solution from 23.47 g MgO/kg P2O5 to 3.29 g MgO/kg P2O5, 48.98 g Al2O3/kg P2O5 to 15.54 g Al2O3/kg P2O5. The removal rates for magnesium and aluminum are found to be 85.98% and 68.27%, respectively, with minimal residue of Na+/F−.

Quantifying the Impacts of an Urban Area on Clouds and Precipitation Patterns: A Modeling Perspective

AbstractOver the past century, rapid urbanization has greatly altered landscapes and affected atmospheric conditions causing impacts across a wide range of sectors including human welfare, infrastructure, and ecosystems. As a result, there is a growing imperative to improve understanding of urbanization impacts on local and regional weather events and hydroclimates. This study investigates how urbanization affects precipitation and cloud fraction (CF) in the vicinity of Indianapolis. We employ multi‐month simulations using the Weather Research and Forecasting model to: (a) assess the impact of an urban area on precipitation and CF, (b) quantify how this impact varies with urban growth, and (c) examine the main mechanisms through which the city alters the local hydroclimate. Specifically, two perturbed simulations, where the urban land cover is either replaced by croplands or is increased in size, are also performed for the two rainiest months. Comparisons of the control run with no city against the perturbed runs indicate statistically significant impacts of the urban area in enhancing precipitation amounts, frequency and low‐level CF, particularly within the first 100 km radius downwind of the city boarder. The urban environment is found to increase precipitation efficiency over the city and in downwind regions. Temperature at 2 m height, planetary boundary layer height, turbulent kinetic energy, and convective available potential energy, are also enhanced in the perturbed runs and drive changes in vertical mixing, downwind precipitation amounts, frequency and low‐level height CF. All these changes appear to be a non‐linear function of the city size.

A novel GNSS and precipitation-based integrated drought characterization framework incorporating both meteorological and hydrological indicators

The Global Navigation Satellite System (GNSS) has become instrumental in developing drought indices, particularly meteorological drought indicators derived from atmospheric precipitable water vapor and hydrological drought indicators based on inverted terrestrial water storage changes. However, these indices traditionally focus on individual aspects of droughts, either meteorological or hydrological droughts, and do not fully capture the integrated nature of drought phenomena. Addressing this gap, this study proposes a novel integrated drought characterization framework using the Gringorten plotting position to derive joint probabilities for GNSS-derived meteorological and hydrological drought indicators. This leads to the creation of a comprehensive multivariate drought severity index (GNSS-MDSI). The analysis across the western United States indicates significant spatial variability in multiyear average precipitation efficiency, ranging from 7.51% to 28.1%. This variability corresponds with marked differences in seasonal terrestrial water storage changes, which oscillate between 25 and 123 mm. Applying this framework in eight states, 9–13 comprehensive drought events from January 2006 to December 2021, with durations spanning from 3 to 54 months, were identified. The GNSS-MDSI not only captured these comprehensive drought periods across various temporal and spatial scales but also aligned closely with drought classifications provided by the US Drought Monitor. These results underscore the utility of this framework in providing a more nuanced and multifaceted perspective on drought conditions, surpassing the capabilities of single-indicator systems. Overall, this study presents an innovative framework for drought monitoring by integrating two GNSS-derived drought indicators, enabling precise and comprehensive delineation of drought characteristics, and offering a geodesy-based solution for integrated global and regional drought monitoring.

Buffering of Aerosol‐Cloud Adjustments by Coupling Between Radiative Susceptibility and Precipitation Efficiency

AbstractAerosol‐cloud interactions (ACI) in warm clouds are the primary source of uncertainty in effective radiative forcing (ERF) during the historical period and, by extension, inferred climate sensitivity. The ERF due to ACI (ERFaci) is composed of the radiative forcing due to changes in cloud microphysics and cloud adjustments to microphysics. Here, we examine the processes that drive ERFaci using a perturbed parameter ensemble (PPE) hosted in CAM6. Observational constraints on the PPE result in substantial constraints in the response of cloud microphysics and macrophysics to anthropogenic aerosol, but only minimal constraint on ERFaci. Examination of cloud and radiation processes in the PPE reveal buffering of ERFaci by the interaction of precipitation efficiency and radiative susceptibility.

Short-Term and Imminent Rainfall Prediction Model Based on ConvLSTM and SmaAT-UNet.

Short-term precipitation forecasting methods are mainly divided into statistical forecasting, numerical model-based forecasting, and radar image extrapolation techniques. The two methods based on statistical prediction and numerical model have the disadvantages of being unstable and generating large errors. Therefore, this study proposes the use of deep learning for radar image extrapolation for precipitation forecasting, in particular by developing algorithms for ConvLSTM and SmaAT-UNet. The ConvLSTM model is a fusion of a CNN (Convolutional Neural Network) and LSTM (Long Short-Term Memory network), which solves the challenge of processing spatial sequence data, which is a task that traditional LSTM models cannot accomplish. At the same time, SmaAT-UNet enhances the traditional UNet structure by incorporating the CBAM (Convolutional Block Attention Module) attention mechanism and replacing the standard convolutional layer with depthwise separable convolution. This innovative approach aims to improve the efficiency and accuracy of short-term precipitation forecasting by improving feature extraction and data processing techniques. Evaluation and analysis of experimental data show that both models exhibit good predictive ability, with the SmaAT-UNet model outperforming ConvLSTM in terms of accuracy. The results show that the performance indicators of precipitation prediction, especially detection probability (POD) and the Critical Success index (CSI), show a downward trend with the extension of the prediction time. This trend highlights the inherent challenges of maintaining predictive accuracy over longer periods of time and highlights the superior performance and resilience of the SmaAT-UNet model under these conditions. Compared with the statistical forecasting method and numerical model forecasting method, its accuracy in short-term rainfall forecasting is improved.

COMPLEX TITANIUM-CONTAINING REAGENTS IN WASTEWATER TREATMENT PROCESSES OF THE FISHING INDUSTRY

The issues of removing nutrients from wastewater from various industries have recently received more and more attention. Traditional coagulants based on aluminum and iron salts can effectively remove phosphate ions from water, but these reagents are not effective against ammonium ions. As part of the work done, the possibility of using complex titanium-containing coagulants in the processes of dephosphatization of wastewater from the fish processing industry was considered. It has been proven that the use of magnesium titanium reagents makes it possible to carry out the coprecipitation of ammonium and phosphate ions in the form of the complex compound of magnesium ammonium phosphate (struvite) with high efficiency. To implement the purification process, a 10–20% stoichiometric excess of magnesium compounds from the calculated value is required, while the efficiency of precipitation of ammonium compounds exceeds 50%, and phosphate anion 95%. The optimal pH range for the process of coprecipitation of ammonium and phosphate ions in the form of a complex compound is 10.0 – 10.5. The results of studies conducted on real water from a fish processing plant demonstrated the increased efficiency of complex titanium-containing reagents compared to traditional coagulants based on aluminum, iron and magnesium salts. The possibility of using magnesium-containing waste from the production of refractory materials has been established both as a precursor for the production of complex coagulants and as an individual reagent. It has been proven that the use of complex titanium-containing reagents makes it possible to significantly intensify the processes of sedimentation (15%) and filtration (20%) of coagulation sludge. The use of complex reagents will significantly increase the level of environmental safety of production and reduce the anthropogenic load on the hydrosphere. For citation: Kuzin E.N. Complex titanium-containing reagents in wastewater treatment processes of the fishing industry. ChemChemTech [Izv. Vyssh. Uchebn. Zaved. Khim. Khim. Tekhnol.]. 2024. V. 67. N 7. P. 127-135. DOI: 10.6060/ivkkt.20246707.6997.

Life Cycle Evolution of Mixing in Shallow Cumulus Clouds

AbstractUnderstanding how entrainment and mixing shape the cloud droplet size distribution (DSD) is crucial for understanding the optical properties and precipitation efficiency of clouds. Different mixing scenarios, mainly homogeneous and inhomogeneous, shape the DSD in a distinct way and alter the cloud's impact on climate. However, the prevalence of these mixing scenarios and how they vary in space and time is still uncertain, as underlying processes are commonly unresolved by conventional numerical models. To overcome this challenge, we employ the L3 model, which considers supersaturation fluctuations and turbulent mixing down to the finest relevant lengthscales, making it possible to represent different mixing scenarios realistically. We investigate the spatial and temporal evolution of mixing scenarios over the life cycle of shallow cumulus clouds for varying boundary layer humidities and aerosol concentrations. Our findings suggest homogeneous mixing is generally predominant in cumulus clouds, while different mixing scenarios occur concurrently in the same cloud. Notably, inhomogeneous mixing increases over the cloud life cycle across all analyzed cases. The mean and standard deviation of supersaturation are found to be the most capable indicators of this evolution, providing a comprehensive insight into the characteristics of mixing scenarios. Finally, we show inhomogeneous mixing is more prevalent in drier boundary layers and for higher aerosol concentrations, underscoring the need for a more comprehensive investigation of how these mixing dynamics evolve in a changing climate.

Preparation of V2O3 by a short process of ammonium salt reduction and its electrocatalytic properties investigation

Ammonium salt vanadium precipitation is the main method of vanadium metallurgy at home and abroad, but the traditional ammonium precipitation of vanadium can only obtain high valence of V2O5, and the NH3 produced in the calcination process cannot be fully utilized. And the preparation of low-valence vanadium oxide V2O3 is long and costly. Self-reduction of thermal decomposition of nitrogen-containing precipitation products to produce V2O3 is a novel and short process method that can achieve full utilization of ammonia. NaVO3 was used as the vanadium source, choline chloride (C5H14ClNO (ChCl)) was used as the vanadium precipitation reagent, and the vanadium concentration was 30 g/L, n(ChCl)/n(V) = 1.5, the initial pH was 1.5, the temperature was 85°C, the time was 2 h, and the vanadium precipitation efficiency could reach 97.05%. V2O3 with purity of 97.98% can be obtained by calcination at 550°C for 3 h in an argon atmosphere. XRD, FTIR, XPS, SEM-EDS were used to characterize the product, which further proved that the final product was V2O3. The electrocatalytic hydrogen evolution reaction (HER) of V2O3 in acidic media showed high stability after 1000 cyclic voltammetry (CV) cycles, with a low Tafel slope of 88.5 mVdec−1. The electrochemical double layer capacitance (EDLC) was measured by CV at different scan rates, and the EDLC of V2O3 was 1.104 mFcm−2. This study provides a short-flow preparation method for V2O3 and can provide predictions for the application of V2O3 in HER and supercapacitors.

Potential Modulation of Aerosol on Precipitation Efficiency in Southwest China

Potential Modulation of Aerosol on Precipitation Efficiency in Southwest China

Efficient and clean treatment of indium-bearing zinc ferrite: A new approach using a water-regulated deep eutectic solvent

Indium, a strategic key metal, is mainly recovered from zinc leaching residue. However, a challenging problem in the treatment of zinc leaching residue is how to efficiently and cleanly treat the poorly soluble phase, the indium-bearing zinc ferrite (IBZF). Here, we propose a novel strategy for the selective extraction of indium, zinc and iron from IBZF. By introducing water to regulate the coordination environment of a choline chloride-anhydrous oxalic acid based deep eutectic solvent (ChCl-Oxa based DES), efficient leaching and deep separation of indium, zinc and iron were achieved. Water, acting as both co-solvent and diluent, played a critical role in the whole process. The addition of a little water reduces the viscosity of the DES, thereby improving the leaching of indium, zinc and iron. Under specific leaching conditions (leaching temperature of 90 °C, liquid/solid ratio of 20:1 ml/g, molar ratio of ChCl:Oxa = 1:1, molar ratio of DES:H2O = 1:3 and reaction time of 3.5 h), the leaching efficiencies of indium, zinc and iron were 96.27 %, 95.55 % and 96.33 %, respectively. The subsequent addition of large amounts of water dilutes and dissociates the structure of the DES, facilitating the stepwise precipitation and deep separation of metal ions. The precipitation efficiency of zinc and iron reached 93.06 % and 98.07 %, respectively. Residual indium ions in the solution were effectively adsorbed by activated carbon, with a recovery efficiency of 75.7 % after five cycles of adsorption. The solution separated from the metal ions was well regenerated by evaporation and addition of Oxa. Our work represents a novel approach for the recycling and clean utilization of zinc leaching residue. And the regulation of the coordination environment of metal ions in DES is proved to have great potential in metal recycling.

Recovery of Vanadium from Carbonated Solution by Extraction with Transformed Aliquat 336 and Stripping-Precipitation

ABSTRACT The leaching solution from the direct reduction-sodium smelting process of ultra-poor vanadium-bearing titanomagnetite contained sodium, vanadium, and impurities. It was carbonated to ensure sodium recovery, and the recovery of vanadium proved challenging. This study proposed and optimized a novel process of extracting vanadium from this alkaline carbonated solution using the transformed organic phase of 20 vol% (0.44 mol/L) Aliquat 336, 8 vol% 2-octanol, and 72 vol% sulfonated kerosene. To achieve superior vanadium extraction, Aliquat 336 in the organic phase was transformed into carbonate type by successively washing with NaHCO3 and NaOH solutions. The single-stage extraction efficiency of vanadium exceeded 85% at a pH of 8.0‒9.0 and an organic-to-aqueous phase ratio of 1:1. The thermodynamics of the vanadium extraction process was examined, confirming that the extraction process is an exothermic reaction (ΔH 0 = −9.56 kJ/mol). Furthermore, using a solution of 4 mol/L NH4Cl and 1 mol/L NH4OH with a pH of 8.8 as the effective stripping reagent to realize one-step stripping and precipitation of vanadium, the single-stage stripping efficiency of vanadium was 99.8%, and the precipitation efficiency was about 95%. Through this stripping-precipitation process, solid ammonium metavanadate with a purity of 98.14% was obtained in one step. The mechanisms of transformation, extraction, and stripping were studied via infrared spectra of the organic phases.

Performance of Co9S8/Cu2S@LDH nanowires prepared by dual sulfur sources to promote oxygen evolution performance

Hydrogen production through water electrolysis is an effective method for obtaining clean energy. The oxygen evolution reaction plays a key role in the process of water electrolysis, and the development of efficient and stable oxygen evolution electrocatalysts is crucial to improve the efficiency of hydrogen production. This paper presents the preparation of Co9S8/Cu2S@LDH nanowire catalysts on copper foam using thiourea and sodium sulfide as dual sulfur sources by a hydrothermal method combined with electrochemical deposition. The composition, structure, and performance of the catalysts were investigated using characterization methods of XRD, SEM, Raman, and electrochemical analyses. Also, the mechanism of thiourea's role in the process of sulfurization of dual sulfur sources was also revealed. The results indicate that the introduction of thiourea facilitates the generation of the active center CoOOH and improves the catalytic efficiency of oxygen precipitation with a low overpotential of 199 mV at 10 mA/cm2 in 1.0 M KOH electrolyte. Additionally, the LDH deposited in the outer layer effectively prevents the oxidation of Co9S8/Cu2S and improves the catalyst's stability. Therefore, the prepared Co9S8/Cu2S@LDH nanowire catalyst exhibits high oxygen evolution reaction (OER) catalytic activity and stability in alkaline electrolyte, making it a promising OER catalyst.

Selective removal of Fe(Ⅲ) from HNO3 leach liquor of saprolitic laterite ore: Precipitation efficiency, hydrolysis mechanism, and removal strategy

HNO3 leach liquor of saprolitic laterite ore contains Fe3+, Al3+, Sc3+, Ni2+, Co2+, Mg2+, etc. Efficient removal of Fe3+ while reducing the loss of valuable metals is the key to solution purification. In this paper, a detailed study was carried out on the removal of Fe3+ by hydrolysis precipitation. First, thermodynamic calculations were conducted to analyze the precipitation pH changes and possible hydrolysis products at different temperatures. Subsequently, the experimental results show that more than 99 % Fe can be removed via low-temperature hydrolysis at 25–80 ℃ and pH = 3.0–3.5, but over 40 % Al and 30 % Sc also precipitate together, causing valuable metals losses. Differently, under high-temperature conditions (180 ℃, pH = 1.0, 0.5 h), 99.49 % of Fe can be selectively removed as Fe2O3 product, while other valuable metals hardly precipitate. It was found that the low-temperature Fe removal residue exhibits irregular agglomeration and uneven particle size, and contains 9.48 % NO3– and 25.86 % Fe. The high-temperature Fe removal residue presents a nanoscale spherical shape with uniform particle size and only contains 0.087 % NO3–, and the Fe content is up to 69.07 %. Finally, the Fe3+ hydrolysis mechanism was analyzed and an efficient Fe removal process from the HNO3 system including pH adjustment of leach liquor, selective Fe removal at high temperature, flash evaporation to recover free HNO3, and solid–liquid separation to obtain Fe2O3 products was proposed.

An integrated process incorporating pH-controlled biomineralization and sulfate bioreduction to facilitate recovery of schwertmannite and sulfated polysaccharides from acid mine drainage

Acid mine drainage (AMD) poses an environmental challenge to the global mining industry. However, it is also a potential source of iron and sulfate for recovery. In this work, we proposed an integrated process of pH-controlled biomineralization of iron and biological sulfate reduction to facilitate the concurrent purification of AMD and selective recovery of iron and sulfate as valuable products. Compared with the biomineralization controlling pH at 2.8, the modified biomineralization controlling pH at 3.2 improved the iron precipitation efficiency from 9.7 % to 95.3 %, leading to a 53–fold increase in pure-grade schwertmannite Fe8O8(OH)4.08(SO4)1.96 production in AMD. This improvement may be related to the relatively low Gibbs free energy value of the Fe3+ mineralization reaction at pH 3.2. The solution equilibrium calculation using Visual MINTEQ indicated that keeping the system pH at an appropriate level is crucial for the spontaneous and effective biomineralization of Fe to schwertmannite at room temperature. The specific pH required for Fe biomineralization is slightly affected by the content of metal cations (e.g., Al3+, Ca2+, Mg2+, Cu2+, and Mn2+) and oxoanion (AsO33−/AsO43−) in AMD but greatly affected by sulfate content (mM) (pH = 0.149 ln c(SO42−) + 2.551). For the effluent resulting from AMD biomineralization, sulfate reduction at pH 5.0 removed residual Zn2+ (99.9 %), Mn2+ (97 %), Cu2+ (100 %), and sulfate (87.1 %) while recovering sulfated polysaccharides with antiangiogenic bioactivity (as confirmed by the chorioallantoic membrane tests). The pilot-scale continuous flow operation experiments demonstrated that this integrated method has the potential to recover valuable iron/sulfate products from AMD.

El Niño‐La Niña Asymmetries in the Changes of ENSO Complexities and Dynamics Since 1990

AbstractIn around 1990, significant shifts occurred in the spatial pattern and temporal evolution of the El Niño‐Southern Oscillation (ENSO), with these shifts showing asymmetry between El Niño and La Niña phases. El Niño transitioned from the Eastern Pacific (EP) to the Central Pacific (CP) type, while La Niña's multi‐year (MY) events increased. These changes correlated with shifts in ENSO dynamics. Before 1990, El Niño was influenced by the Tropical Pacific (TP) ENSO dynamic, shifting to the Subtropical Pacific (SP) ENSO dynamic afterward, altering its spatial pattern. La Niña was influenced by the SP ENSO dynamic both before and after 1990 and has maintained the CP type. The strengthened SP ENSO dynamic since 1990, accompanied by enhanced precipitation efficiency during La Niña, make it easier for La Niña to transition into MY events. In contrast, there is no observed increase in precipitation efficiency during El Niño.

Polydispersity-mediated high efficacy of an in-situ aqueous nanosuspension of PPEF.3HCl in methicillin resistant Staphylococcus aureus sepsis model

Recently, World Health Organization declared antimicrobial resistance as the third greatest threat to human health. Absence of known cross-resistance, new class, new target, and a new mode of action are few major strategies being undertaken by researches to combat multidrug resistant pathogen. PPEF.3HCl, a bisbenzimidazole was developed as highly potent antibacterial agent against ESKAPE (Enterococcus faecium, Staphylococcus aureus, Klebsiella pneumoniae, Acinetobacter baumannii, Pseudomonas aeruginosa, and Enterobacter species) pathogens, targeting topoisomerase IA. The present work encompasses a radical on-site generation of In-situ nanosuspension of PPEF.3HCl with enhanced efficacy against methicillin resistant S. aureus in septicemia model. We have generated instantaneously a PPEF.3HCl nanosuspension (IsPPEF.3HCl-NS) by mixing optimized monophasic PPEF.3HCl preconcentrate in propylene glycol into an aqueous medium comprising tween 80 as stabilizer. The IsPPEF.3HCl-NS showed precipitation efficiency of > 90 %, average particle size < 500 nm, retained upto 5 h, a negative zeta potential and bi/trimodal particle size distribution. Differential scanning calorimetry, X-ray diffraction confirmed partial amorphization and transmission electron microscopy revealed spherical particles. IsPPEF.3HCl-NS was non-hemolytic and exhibited good stability in serum. More significantly, it exhibited a ∼ 1.6-fold increase in macrophage uptake compared to free PPEF.3HCl in the RAW 264.7 macrophage cell line. Confocal microscopy revealed accumulation of IsPPEF.3HCl-NS within the lysosomal compartment and cell cytosol, proposing high efficacy. In terms of antimicrobial efficacy, IsPPEF.3HCl-NS outperforms free PPEF.3HCl against clinical methicillin sensitive and resistant S. aureus strains. In a pivotal experiment, IsPPEF.3HCl-NS exhibited over 83 % survival at 8 mg/kg.bw and an impressive reduction of ∼ 4–5 log-fold in bacterial load, primarily in the kidney, liver and spleen of septicemia mice. IsPPEF.3HCl-NS prepared by the In-situ approach, coupled with enhanced intramacrophage delivery and superior efficacy, positions IsPPEF.3HCl-NS as a pioneering and highly promising formulation in the battle against antimicrobial resistance.

Assessment of the Spraying System Impact at the Preparation Stage of Iron Ore Concentrate Products

Introduction. Harmful components of ore dust, formed during the unloading of products in the preparation of iron ore concentrate (PPIOC) at the mixing stage, cause damage to both workers and equipment. To address this issue, liquid aerosol spraying using nozzles with large diameters (&gt;20 μm) is used. However, this method proves ineffective in capturing fine-dust particles. Therefore, enhancing the efficiency of the dust deposition method through PPIOC dust spraying becomes a pressing challenge. The aim of this study is to investigate the impact of the Dry Fog technology, generating liquid droplets up to 20 μm in size, during the unloading stage of PPIOC at a mining and metallurgical enterprise in the precipitation of suspended fine-dust particles. The primary goal of this research was to assess the effectiveness and potential advantages of applying the Dry Fog technology for dust spraying with subsequent precipitation, as this technology has not been previously applied to PPIOC dust.Materials and Methods. The experiment on the PPIOC dust deposition was conducted in a specially designed laboratory setup. Through physical modeling in the laboratory setup, parameters of the precipitation process were obtained. Subsequently, the results were analyzed to understand the dependence of dust precipitation over time, taking into account the influence of the Dry Fog technology. An experiment program was developed for physical modeling. According to the devised program, dust was uniformly loaded into the interior of the laboratory setup (from the top), distributed in the air stream throughout the volume of the setup by a fan, and an instrument located at the bottom recorded changes in concentration over time. Experiments on dust precipitation were then conducted using liquid spraying (filtered water as the liquid) introduced into the setup through nozzles generating droplets with sizes of 10 and 15 μm, concurrently with the loading of dust into the laboratory setup. The effectiveness of the Dry Fog technology in the deposition of PPIOC dust was determined visually and further analyzed based on a comparison of graphs. The dynamics of changes in the average dust concentrations depending on time was studied both during precipitation without spraying and using the Dry Fog technology. During the experiment, the characteristics of the microclimate inside the laboratory setup (humidity, temperature and air velocity) and the parameters of two nozzles — their operating pressure and the supplied liquid spraying time — were recorded.Results. The comparison of the results showed a reduction in the dust precipitation time by 40 % and 75 % when using nozzles with sizes of 10 μm and 15 μm, respectively.Discussion and Conclusion. The experiment results confirm the effectiveness of the Dry Fog technology for PPIOC dust precipitation during unloading at the mixing stage. Fundamental findings have been obtained, providing a basis for further assessment of the efficiency of dust precipitation with the additional application of pulsating ventilation. In such a combination, an additional 20–25 % increase in precipitation efficiency is anticipated compared to the results presented in this article. The obtained results will support the justification of rational parameters and the implementation of the described method in production to enhance dust precipitation efficiency. Additionally, they will aid in developing a methodology to accelerate the PPIOC dust precipitation using the pulsating ventilation method.

Sulfate Doping Promotes Agglomeration of Calcium Fluoride Crystals.

Calcium salt precipitation is an effective solution to wastewater fluoride pollution. The purity and precipitation efficiency of calcium fluoride is critical for its removal and recovery. This study aimed to reveal the role of coexisting sulfates in the precipitation of calcium fluoride. A low sulfate concentration promoted calcium fluoride precipitation. The size of calcium fluoride-aggregated particle clusters increased from 750 to 2000 nm when the molar ratio of sulfate to fluoride was increased from 0 to 3:100. Sulfate doped in the calcium fluoride crystals neutralized the positive charge of the calcium fluoride. Online atomic force microscopy measurements showed that sulfate reduced the repulsive force between calcium fluoride crystals and increased the adhesion force from 1.62 to 2.46 nN, promoting the agglomeration of calcium fluoride crystals. Sulfate improved the precipitation efficiency of calcium fluoride by promoting agglomeration; however, the purity of calcium fluoride was reduced by doping. Sulfate reduced the induction time of calcium fluoride crystallization and improved the nucleation rate of calcium fluoride. Sulfate should be retained to improve the precipitation of calcium fluoride and to avoid its loss from the effluents. However, it is necessary to separate sulfate from fluoride to obtain high-purity calcium fluoride. Therefore, sulfate concentration regulation in high-fluoride wastewater is key to achieving the efficient removal and recovery of fluoride ions.