Efficiency Of Pig Production Research Articles

Transcriptome Analysis Reveals the Early Development in Subcutaneous Adipose Tissue of Laiwu Piglets.

Adipose tissue plays an important role in pig production efficiency. Studies have shown that postnatal development has a vital impact on adipose tissue; however, the mechanisms behind pig adipose tissue early-life programming remain unknown. In this study, we analyzed the transcriptomes of the subcutaneous adipose tissue (SAT) of 1-day and 21-day old Laiwu piglets. The results showed that the SAT of Laiwu piglets significantly increased from 1-day to 21-day, and transcriptome analysis showed that there were 2352 and 2596 differentially expressed genes (DEGs) between 1-day and 21-day SAT in male and female piglets, respectively. Expression of genes in glycolysis, gluconeogenesis, and glycogen metabolism such as pyruvate kinase M1/2 (PKM), phosphoenolpyruvate carboxy kinase 1 (PCK1) and amylo-alpha-1, 6-glucosidase, 4-alpha-glucanotransferase (AGL) were significantly different between 1-day and 21-day SAT. Genes in lipid uptake, synthesis and lipolysis such as lipase E (LIPE), acetyl-CoA carboxylase alpha (ACACA), Stearoyl-CoA desaturase (SCD), and 3-hydroxy-3-methylglutaryl-CoA synthase 1 (HMGCS1) were also differentially expressed. Functional analysis showed enrichment of DEGs in transcriptional regulation, protein metabolism and cellular signal transduction. The protein-protein interaction (PPI) networks of these DEGs were analyzed and potential hub genes in these pathways were identified, such as transcriptional factors forkhead box O4 (FOXO4), CCAAT enhancer binding protein beta (CEBPB) and CCAAT enhancer binding protein delta (CEBPD), signal kinases BUB1 mitotic checkpoint serine/threonine kinase (BUB1) and cyclin-dependent kinase 1 (CDK1), and proteostasis-related factors ubiquitin conjugating enzyme E2 C (UBE2C) and cathepsin D (CTSD). Moreover, we further analyzed the transcriptomes of SAT between genders and the results showed that there were 54 and 72 DEGs in 1-day and 21-day old SAT, respectively. Genes such as KDM5D and KDM6C showed gender-specific expression in 1-day and 21-day SAT. These results showed the significant changes in SAT between 1-day and 21-day in male and female Laiwu pigs, which would provide information to comprehensively understand the programming of adipose tissue early development and to regulate adipose tissue function.

446 A stochastic, dynamic agent-based model of swine nutrient requirements for the growing finishing production phase

Abstract Agent-based modeling (ABM) is a powerful tool that offers the flexibility to represent the stochasticity exhibited by swine systems due to individual animal nutritional requirements. ABM can also accurately mimic the biological dynamic growth performance of pigs. Addressing and simulating the biological growth dynamics enables precision nutrition and sustainable production management. To realistically replicate the nutrient requirement and growth performance of growing-finishing pigs, we developed an ABM based on the principles and equations from the eleventh Swine Nutrient Requirement Council Model. Each individual pig (agent) in the model had a starting weight of 20 kg and a fixed finishing weight of 140 kg, with a variable number of days on feed. Our model considers three sexes: gilt, barrow, and intact male. Pigs move through the farm, feed, and interact with the environment. Our model dynamically simulates the complex interactions between feed intake, metabolism, and growth processes of growing pigs, thereby being a virtual visualizable growing-finishing swine farm (Figure 1). The basic parameters of the biological growth of pigs, including the body weight, feed intake, metabolizable energy (ME) intake, maintenance ME requirements, protein deposition (Pd), lipid deposition (Ld), and probe backfat thickness (Figure 2), were modeled based on NRC equations. In addition to the above parameters, the daily amino acid, calcium (Ca), and phosphorus (P) requirements for individual pigs were calculated daily. To demonstrate ABM responsiveness to feeding strategies, ractopamine supplementation is included to evaluate the impact on protein deposition. The ABM offers more personalized nutrition management by considering unique pig growth potential and nutritional needs based on characteristics such as age and sex, which can lead to improved growth performance and feed efficiency. Besides, the ABM enables the modeling of population-level dynamics and the evaluation of different feeding scenarios to optimize growth outcomes by allocating optimized feed based on individual nutritional needs. Our ABM will be used to test different feeding management strategies and scenarios, providing valuable insights into the impact of various factors on pig nutrition and growth outcomes while minimizing both time and financial expenditures. Our ABM aims to provide a robust framework for simulating and optimizing feeding strategies and enhancing overall pig production efficiency by leveraging the comprehensive and scientifically validated guidelines provided in the NRC. To verify the accuracy and reliability of the proposed ABM, the results generated by the model were rigorously evaluated and compared with the NRC model results. The results demonstrated a highly satisfactory prediction performance by our ABM, paving the way for more real-time decisions to be made for daily feed management on growing-finishing swine operations.

Lysine requirement of weaned piglets

Lysine, often referred to as the ‘first limiting amino acid’ in pig nutrition, plays a pivotal role in growth performance. Variability in lysine requirements arises due to factors such as age, sex and environmental conditions. Optimising pig health and production efficiency and minimising nitrogen excretion require accurate knowledge of estimated lysine requirements accounting for factors such as genetics, feeding practices, scientific advancements, and environmental considerations. This study aimed to determine standardised ileal digestible (SID) lysine requirements of weaned piglets (5–30 kg) based on a literature review using meta-analytical approaches. The literature review yielded 344 studies that were screened for title and abstract. In total, 41 experiments met the inclusion criteria, resulting in a dataset of 206 treatment means. Linear, quadratic and linear-plateau models were used to gain insight into the effect of SID lysine addition on average daily gain and feed efficiency for the combined dataset and separately for the individual experiments. Regression analysis showed a predominant linear increase in average daily gain and feed efficiency as an overall response to increasing lysine levels across both the combined dataset and individual experiments. Breakpoint estimation from the linear-plateau models was inconclusive, indicating that the optimal SID lysine requirement to maximise piglet growth performance likely exceeded the upper lysine levels tested in most studies, thus surpassing 1.3 g SID lysine per MJ net energy. This review indicates high values for the lysine requirement to achieve maximum growth performance. Results may suggest that piglet feed formulation should focus on an optimal dietary SID lysine to CP ratio, rather than SID lysine per kg of diet or unit of net energy. However, more research is needed to investigate this suggestion.

Buserelin Acetate Added to Boar Semen Enhances Litter Size in Gilts in Tropical Environments.

The use of exogenous hormones has long been of interest for improving reproductive performance in swine production. Enhancing litter size directly impacts the economic efficiency of pig production. Various strategies, including nutritional, genetic, and hormonal approaches, have been explored with varying degrees of success. Administering a gonadotropin-releasing hormone (GnRH) agonist, such as buserelin, at the onset of estrus can induce ovulation and reduce the variation in ovulation timing among sows. This study assessed the impact of GnRH agonist supplementation in boar semen doses on the litter size of inseminated gilts. The research was conducted on a commercial swine herd in northern Thailand. A total of 231 Landrace × Yorkshire crossbred gilts, aged 224.5 ± 16.2 days at the onset of estrus synchronization, participated in the experiment. The gilts' estrus was synchronized with oral altrenogest supplementation at a dosage of 20 mg/day for 18 days. After exhibiting standing estrus, the gilts were randomly divided into three groups. Control group: gilts were inseminated at 0 and 12 h post standing estrus onset with a conventional semen dose (n = 94). Treatment 1: similar to the control group, but with an added 5 µg (1.25 mL) of buserelin acetate to the boar semen dose during the first insemination (n = 71). Treatment 2: similar to the control group, but with 10 µg (2.5 mL) of buserelin acetate added to the boar semen dose during the first insemination (n = 66). All gilts were inseminated twice during their standing estrus using the intrauterine artificial insemination method. Each semen dose contained 3.0 × 109 motile sperm in 80 mL. The farrowing rate averaged 78.8% and did not significantly differ between the groups (p = 0.141). The total number of piglets born per litter in the treatment 2 group was greater than in the control group (14.0 ± 0.3 vs. 13.2 ± 0.3, respectively, p = 0.049), but was not significantly different from the treatment 1 group (13.3 ± 0.3, p = 0.154). Similarly, the number of live-born piglets in the treatment 2 group was greater than in the control and treatment 1 groups (13.2 ± 0.4 vs. 12.3 ± 0.3 and 12.0 ± 0.4, respectively, p < 0.05). Moreover, the live-born piglets' litter birth weight in the treatment 2 group was greater than in the control group (17.0 ± 0.4 vs. 15.6 ± 0.3 kg, respectively, p = 0.008) and the treatment 1 group (15.7 ± 0.4 kg, p = 0.025). In conclusion, adding a GnRH agonist to boar semen appears to enhance the litter size of gilts. Further research should focus on understanding the underlying mechanisms and determining the optimal dose and timing for GnRH agonist supplementation.

Resource Use Efficiency of Pig Production raised with and without Open Area during Summer Season

A total of 20 piglets (10 castrated males and 10 females) at 3 months of age with initial body weight of 18.94 ± 1.04 Kg were randomly subjected to minimum limit of floor area recommendations of housing standards (IS: 3916-1966) range of 0.9-1.8 square meter for weaner to finisher. In treatments Tc, pigs were provided with only 0.9 m2 covered floor area per pig without access to open area and in control (To) with covered floor area of 0.9 m2 plus additional equal open floor area (total 1.8 m2 per pig). Result showed that resource use efficiency in terms of saving of 90 % space, 41.23 % labor and 22.05 % water per pig in Tc were significantly (p &lt; 0.01) better than To. However, production cost per kg live weight in Tc was 5.38 % higher than To.

Current issues of animal welfare and health in pig breeding (review)

Objective. To summarise current issues related to welfare and health in pig production. Methods. Domestic and foreign sources of literature on the subject of research and their desk analytical analysis. Results. Today, animal welfare is determined by a combination of three interrelated components: the physical, mental and natural state of certain animals, as well as, of course, humane human attitude to meeting their needs. Until recently, the problems of humane treatment ofproductive animals were exclusively related to economically developed countries where intensive livestock production technologies were used. Pig farms need to have highly productive animals, adequate and balancedfeed, and use resource-saving technologies to produce breeding and marketable products at the optimum cost. Improving the efficiency of pig production primarily has a negative impact on the welfare of pigs. At the same time, it should be understood that, in general, pigs are raised to produce meat and lard products, which is directly related to their slaughter and, accordingly, the taking of their lives. Therefore, the needs of pigs will not be fully met. However, the basic needs - the so-calledfive freedoms of animals - must be met. A detailed grouping of approaches to determining animal welfare is formed in three main aspects: physical condition, mental condition and naturalness. It should be borne in mind that the satisfaction of specific needs in the natural environment does not always ensure their full satisfaction in the two aspects ofphysical and mental health. In the case of pig breeding, it is necessary to take into account the consequences of long term targeted selection of the main breeds and significant modification of their representatives compared to wild forms. The provision of air, water and feed for pigs is critical and should be provided in full. However, this is not always taken into account on Ukrainian pig farms. In addition, the welfare of pigs is inextricably linked and directly depends on the health of the animals. Conclusions. The review of literature sources to summarise current issues related to welfare in pig production indicates that there are a number of issues to be addressed in Ukraine. First and foremost, we are talking about EU legislation that should be implemented in domestic production, which is especially relevant on Ukraine's path to full EU membership. With regard to the existing pig welfare issues, the list of methods and approaches for determining certain indicators is gradually expanding, but it should be borne in mind that welfare in pig production is not limited to pig housing. These are issues related to the transport and slaughter of pigs, aspects that synergise with the subsequent safety of pig products. Of particular importance are the issues in which pig welfare intersects with biosecurity and ensuring appropriate animal health. Key words: pigs, welfare, animal health, housing, feeding, productivity, commercial pork production.

Environmental Efficiency of Pig Production in Chuong My District, Hanoi

Environmental Efficiency of Pig Production in Chuong My District, Hanoi

PSIII-2 Pediococcus Acidilactici Demonstrates Probiotic Properties and Inhibits the Growth of Enterotoxigenic Escherichia Coli

Abstract Lactic acid bacteria are recognized to possess probiotic properties including modulating host physiology. Probiotics are used as direct-fed microbials in the swine industry to optimize pig performance and production efficiency. In the nursery, probiotics have been shown to mitigate post-weaning diarrhea caused by enterotoxigenic Escherichia coli (ETEC). Studies show beneficial effects of supplementing probiotics and the data were inconsistent. Microbial genome sequencing could help better define the mechanisms of probiotic mode of action and dose required to improve probiotic efficacy. The aim of this study was to determine if Pediococcus acidilactici PECh3A (PA) that has been genome sequenced for determining gut physiology mechanisms expresses probiotic properties in-vitro. The growth curve of PA was conducted to determine colony forming units (CFU)/mL concentrations in 2-hour intervals until plateau at 15 hours. Based on the results of the growth curve dose response analysis were performed for acid and bile tolerance and antimicrobial activity against ETEC (3030-2: K88ac LT and STb). Doses investigated were 1010, 109, and 108 CFU/mL. For the acid and bile tolerance experiment PA was grown to concentration 1010 CFU/mL and was centrifuged and inoculated in De Mab, Rogosa, and Sharpe broth (MRS Broth) at pH 2, 4, or 6.5 (control) or MRS ± 0.3% oxgall (wt/vol), respectively, and incubated for 4 h. Following the 4 h incubation samples were serially diluted, plated, and incubated at 37°C for 24 h to determine percent survival. Antimicrobial inhibition of ETEC was assessed by the agar zone of inhibition (ZOI) methods. PA was grown to previously defined CFU/mL concentrations and 100 µL of the culture and filtered supernatant were plated into wells on nutrient agar plates spread with 107 ETEC CFU/mL. ZOI were measured in mm. Data were analyzed according to a factorial design using PROC MIXED procedures in SAS. Survival in acidic media relative to control media was observed in all doses (108,109, and 1010) at pH 4 and minimally at pH 2 (81.8, 90.6, and 89.5% or 33.8, 28.0, and 0% ±7.7, respectively; P &amp;lt; 0.01). PA survival in 0.3% oxgall increased with increasing dose (74.3, 83.3, and 91.7% ±0.67; P &amp;lt; 0.01). PA culture exhibited antimicrobial activity against ETEC at all doses tested, and the PA filtered supernatant exhibited antimicrobial activity from 109 CFU/mL (4.260 mm ± 0.9437, P &amp;lt; 0.01). Taken together the findings indicate that PA PECh3A exhibits functional probiotic properties to potentially improve swine intestinal physiology. Further in vitro and in vivo experiments will access Pediococcus acidilactici PECh3A function and interaction within the host environment.

FGF2, LIF, and IGF1 (FLI) supplementation during human in vitro maturation enhances markers of gamete competence.

Does a chemically defined maturation medium supplemented with FGF2, LIF, and IGF1 (FLI) improve in vitro maturation (IVM) of cumulus-oocyte complexes (COCs) obtained from children, adolescents, and young adults undergoing ovarian tissue cryopreservation (OTC)? Although FLI supplementation did not increase the incidence of oocyte meiotic maturation during human IVM, it significantly improved quality outcomes, including increased cumulus cell expansion and mitogen-activated protein kinase (MAPK) expression as well as enhanced transzonal projection retraction. During OTC, COCs, and denuded oocytes from small antral follicles are released into the processing media. Recovery and IVM of these COCs is emerging as a complementary technique to maximize the fertility preservation potential of the tissue. However, the success of IVM is low, especially in the pediatric population. Supplementation of IVM medium with FLI quadruples the efficiency of pig production through improved oocyte maturation, but whether a similar benefit occurs in humans has not been investigated. This study enrolled 75 participants between January 2018 and December 2021 undergoing clinical fertility preservation through the Fertility & Hormone Preservation & Restoration Program at the Ann & Robert H. Lurie Children's Hospital of Chicago. Participants donated OTC media, accumulated during tissue processing, for research. Participants who underwent OTC and include a pediatric population that encompassed children, adolescents, and young adults ≤22 years old. All participant COCs and denuded oocytes were recovered from media following ovarian tissue processing. IVM was then performed in either a standard medium (oocyte maturation medium) or one supplemented with FLI (FGF2; 40 ng/ml, LIF; 20 ng/ml, and IGF1; 20 ng/ml). IVM outcomes included meiotic progression, cumulus cell expansion, transzonal projection retraction, and detection of MAPK protein expression. The median age of participants was 6.3 years, with 65% of them classified as prepubertal by Tanner staging. Approximately 60% of participants had been exposed to chemotherapy and/or radiation prior to OTC. On average 4.7 ± 1 COCs and/or denuded oocytes per participant were recovered from the OTC media. COCs (N = 41) and denuded oocytes (N = 29) were used for IVM (42 h) in a standard or FLI-supplemented maturation medium. The incidence of meiotic maturation was similar between cohorts (COCs: 25.0% vs 28.6% metaphase II arrested eggs in Control vs FLI; denuded oocytes: 0% vs 5.3% in Control vs FLI). However, cumulus cell expansion was 1.9-fold greater in COCs matured in FLI-containing medium relative to Controls and transzonal projection retraction was more pronounced (2.45 ± 0.50 vs 1.16 ± 0.78 projections in Control vs FLIat 16 h). Additionally, MAPK expression was significantly higher in cumulus cells obtained from COCs matured in FLI medium for 16-18 h (chemiluminescence corrected area 621,678 vs 2,019,575 a.u., P = 0.03). Our samples are from human participants who exhibited heterogeneity with respect to age, diagnosis, and previous treatment history. Future studies with larger sample sizes, including adult participants, are warranted to determine the mechanism by which FLI induces MAPK expression and activation. Moreover, studies that evaluate the developmental competence of eggs derived from FLI treatment, including assessment of embryos as outcome measures, will be required prior to clinical translation. FLI supplementation may have a conserved beneficial effect on IVM for children, adolescents, and young adults spanning the agricultural setting to clinical fertility preservation. This work was supported by Department of Obstetrics and Gynecology startup funds (F.E.D.), Department of Surgery Faculty Practice Plan Grant and the Fertility & Hormone Preservation & Restoration Program at the Ann & Robert H. Lurie Children's Hospital of Chicago (M.M.L. and E.E.R.). M.M.L. is a Gesualdo Foundation Research Scholar. Y.Y.'s research is supported by the internal research funds provided by Colorado Center of Reproductive Medicine. Y.Y., L.D.S., R.M.R., and R.S.P. have a patent pending for FLI. The remaining authors have no conflict of interest. N/A.

Using Recombinant Superoxide Dismutase to Control Oxidative Stress in the Gastrointestinal Tract of Cyclic Heat-Stressed Pigs.

Climate change is associated with an increased frequency and intensity of heat waves, posing a threat of heat stress to pig production. Heat stress compromises the efficiency of pig production partly due to causing oxidative stress, intestinal dysfunction, and inflammatory responses. Superoxide dismutase is an antioxidant enzyme reported to reduce oxidative stress and inflammation. Therefore, this experiment aimed to investigate whether recombinant superoxide dismutase (rSOD) could ameliorate oxidative stress and inflammatory responses in heat-stressed grower pigs. Sixty-four female pigs (Large White × Landrace, 27.8 ± 1.65 kg, mean ± SD) were randomly allocated to a control diet (standard grower feed, CON) or the control diet supplemented with 50 IU recombinant superoxide dismutase (rSOD) for 14 days. After acclimation to the diet, pigs were then housed under thermoneutral (TN, 20 °C, 35-50% relative humidity) or cyclic heat stress conditions (CHS, at 35 °C: 9 a.m. to 5 p.m. and 28 °C: 5 p.m. to 9 a.m., 35-50% relative humidity) for 3 days. Heat stress increased respiration rate (RR), skin and rectal temperature (RR and RT) (p < 0.001 for all), and reduced plasma thyroid hormone concentration (p < 0.001). The amount of oxidized glutathione (GSH:GSSG) was increased in the jejunum and ileum of CHS pigs. In the jejunum, rSOD also increased the amount of oxidized glutathione in both TN and CHS pigs, without any change in endogenous SOD activity. In the ileum, rSOD prevented increases in oxidized glutathione formation in the CHS pigs only. Taken together, this may reflect increased oxidative stress in both the jejunum and ileum in CHS pigs. Alternatively, rSOD increased the conversion of reduced to oxidized glutathione independently of CHS, possibly reflecting an increased overall SOD activity due to the addition of exogenous SOD. In conclusion, the use of in-feed SOD enzymes at a dose of 50 IU/kg may be a useful strategy for preventing oxidative stress in pigs.

Comprehensive analysis of transcriptomic and metabolomic profiles uncovered the age-induced dynamic development pattern of subcutaneous fat in Ningxiang pig

Enhancing meat production and quality is the eternal theme for pig breeding industries. Fat deposition has always been the focus of research in practical production because it is closely linked to pig production efficiency and pork quality. In the current study, multi-omics techniques were performed to explore the modulatory mechanisms of backfat (BF) accumulation at three core developmental stages for Ningxiang pigs. Our results identified that 15 differentially expressed genes (DEGs) and 9 significantly changed metabolites (SCMs) contributed to the BF development via the cAMP signaling pathway, regulation of lipolysis in adipocytes, and biosynthesis of unsaturated fatty acids. Herein, we found a series of candidate genes such as adrenoceptor beta 1 (ADRB1), adenylate cyclase 5 (ADCY5), ATPase Na+/K+ transporting subunit beta 1 (ATP1B1), ATPase plasma membrane Ca2+ transporting 3 (ATP2B3), ATPase Na+/K+ transporting subunit alpha 2 (ATP1A2), perilipin 1 (PLIN1), patatin like phospholipase domain containing 3 (PNPLA3), ELOVL fatty acid elongase 5 (ELOVL5) and metabolites like epinephrine, cAMP, arachidonic acid, oleic acid, linoleic acid, and docosahexaenoic acid existed age-specificeffects and played important roles in lipolysis, fat accumulation, and fatty acid composition. Our findings provide a reference for molecular mechanisms in BF tissue development and the optimization of carcass quality.

Distribution and intensification of pig production in China 2007–2017

Driven by population growth and rising incomes, the demand for animal source foods in low and middle-income countries is increasing rapidly. Pork is one of the most commonly consumed animal-based food, with the highest demand being in China due to its largest population and changing dietary habits linked to increasing wealth. Here, we show the changes in pig production systems in terms of farms capacity, productivity and production at the national and provincial levels by analyzing several censuses of China. In addition, we used a downscaling methodology to provide a recent and highly detailed map of the distribution of pigs in China. Between 2007 and 2017, pork production in China increased by 26.6%, up to 55 million tons and the number of large-scale farms with a yearly production of over 10 000 heads increased by 145%. Much of the production has changed from extensive backyard subsistence farming to intensive corporate farming. Moreover, the pig distribution has shifted from watercourse-intense southeast to northeast and southwest of China due to environmental policy in 2015. These policy-driven transitions primarily aimed to increase pig production efficiency and reduce environmental impacts and resulted in a profound transformation of geographic production patterns.

Scale Difference from the Impact of Disease Control on Pig Production Efficiency.

Simple SummaryThis paper uses the data of fixed observation points in the country’s rural areas, the Data Envelopment Analysis model is used to calculate the production efficiency of pig farmers, which shows the efficiency level of pig breeding in the country. Then, the Tobit model is used to analyze that epidemic prevention and control has a positive effect on production efficiency. Finally, the threshold regression model was used to analyze the results: the effect of disease control on production efficiency was different under different breeding scales. When the breeding scale was less than 6.0002, the disease control had a negative impact on the production efficiency. When the breeding scale was greater than 12.9994, the disease control had a positive impact on the production efficiency. The research results of this paper not only help farmers to correctly understand the relationship between epidemic prevention and production efficiency, guide farmers to expand their breeding scale, and actively participate in epidemic prevention and control, but also provide a reference for the government to formulate policies according to different scales to guide farmers to carry out epidemic prevention and control.Epidemic disease prevention plays a critical role in ensuring the healthy development of livestock farming, and the subjective willingness of breeders can be affected by the cost of epidemic disease prevention. To correct the misconception that farmers regard the cost of disease control as an ineffective cost, and to promote the healthy development of the pig breeding industry, our study employed the data envelopment analysis super-efficiency model and panel threshold regression model to evaluate the combination of the cost of epidemic disease prevention and swine productivity using data collected from 1998–2018 across 30 provinces in China. The following results were obtained. (1) The cost of epidemic disease prevention generated a non-linear on swine productivity when the swine farming scale was limited; (2) When the number of animals at the beginning of the year was less than 6.0002, swine productivity was impacted negatively; (3) When the number of animals at the beginning of the year ranged between 6.0002 and 12.9994, the impact was insignificant; (4) A strong correlation was observed between the expenses of epidemic disease prevention and animal productivity when the number of animals at the beginning of the year exceeded 12.9994. These results indicate that publicity should be enhanced to elucidate the combination of epidemic disease prevention and swine productivity among breeders. In addition, the government should introduce relevant policies to encourage the development of large-scale pig farming, such as subsidies for the construction of large-scale farms and insurance.

Porcine Circovirus Type 2 Vaccines: Commercial Application and Research Advances.

Porcine circovirus type 2 (PCV2) infection can lead to porcine circovirus-associated disease (PCVAD), causing great economic losses to the global swine industry. Conventional vaccination programs are a major measure in the prevention and control of this disease. Currently, there are 5 commercially available PCV2 vaccines in the international market and 10 kinds commercially available PCV2 vaccines in the Chinese market that confer good efficacy against this virus by alleviating clinicopathological manifestations and enhancing growth performance in pigs. In addition, diverse experimental PCV2 vaccines with protective efficiency have been developed, including attenuated chimeric, nucleic acid, subunit, multivalent, and viral-vectored vaccines. These experimental vaccines have been shown to be relatively effective in improving the efficiency of pig production and simplifying prevention procedures. Adjuvants can be used to promote vaccines with higher protective immunity. Herein, we review the application of multiple commercial vaccines over the years and research advances in experimental vaccines, which provide the possibility for the development of superior vaccines to successfully prevent and control PCV2 infection in the future.

Simulating the Commercial Implementation of Gene-Editing for Influenza A Virus Resistance in Pigs: An Economic and Genetic Analysis.

The development of swine Influenza A Virus resistance along with genetic technologies could complement current control measures to help to improve animal welfare standards and the economic efficiency of pig production. We have created a simulation model to assess the genetic and economic implications of various gene-editing methods that could be implemented in a commercial, multi-tiered swine breeding system. Our results demonstrate the length of the gene-editing program was negatively associated with genetic progress in commercial pigs and that the time required to reach fixation of resistance alleles was reduced if the efficiency of gene-editing is greater. The simulations included the resistance conferred in a digenic model, the inclusion of genetic mosaicism in progeny, and the effects of selection accuracy. In all scenarios, the level of mosaicism had a greater effect on the time required to reach resistance allele fixation and the genetic progress of the herd than gene-editing efficiency and zygote survival. The economic analysis highlights that selection accuracy will not affect the duration of gene-editing and the investment required compared to the effects of gene-editing-associated mosaicism and the swine Influenza A Virus control strategy on farms. These modelling results provide novel insights into the economic and genetic implications of targeting two genes in a commercial pig gene-editing program and the effects of selection accuracy and mosaicism.

Using Machine Learning to Identify Biomarkers Affecting Fat Deposition in Pigs by Integrating Multisource Transcriptome Information

Fat deposition in pigs is not only closely related to pig production efficiency and pork quality but also an ideal model for human obesity. Transcriptome sequencing is widely used to study fat deposition. However, due to small sample sizes, high false positive rates, and poor consistency of results from different studies, new strategies are urgently needed. Machine learning, a new analysis method, can effectively fit complex data and accurately identify samples and genes. In this study, 36 samples of adipose tissue, muscle tissue, and liver tissue were collected from Songliao black pigs and Landrace pigs, and the mRNA of all the samples was sequenced. In addition, we collected transcriptome data for 64 samples in the GEO database from four different sources. After standardization and imputation of missing values in the data set comprising 100 samples, traditional differential expression analysis was carried out, and different numbers of expressed genes were selected as features for the training model of eight machine learning methods. In the 1000 replications of fourfold cross validation with 100 samples, AdaBoost performed best, with an average prediction accuracy greater than 93% and the highest mean area under the curve in predicting the high- and low-fat content groups among the eight ML methods. According to their performance-based ranks inferred by AdaBoost, 12 genes related to fat deposition were identified; among them, FASN and APOD were specifically expressed in adipose tissue, and APOA1 was specifically expressed in the liver, which could be important candidate biomarkers affecting fat deposition.

Profitability and efficiency of pig production in Tamil Nadu

The present study was conducted to explore the cost and returns, profitability in pig (Swine) farming and to analyze the various factors affecting pig production and their level of efficiency in pig production. For the study, a sample of 45 swine farms was purposively selected by a simple random sampling procedure in the North-eastern zone of Tamil Nadu. The data were collected by personal interview method with the help of a pre-tested questionnaire pertaining to the year 2013–2014. The results revealed that minimum cost of production and maximum net return per farm per year from the pig rearing was noticed in large farms. Overall benefit-cost ratio was 1.46, which revealed profitable nature of swine farms in the study area. The results of cobb-douglas production function revealed that the variables, feed, and veterinary care were significant and positively influence the pork production. Allocative efficiency analysis showed that the resource feed was over-utilized and veterinary care was under-utilized. Technical efficiency was calculated using stochastic frontier production function and mean technical efficiency was identified as 76.37% and hence 23.63% of their technical abilities were not realized. Multiple regression was used to identify the factors influencing farm specific technical efficiency and profitability. Thus, the efficiency in pig production in the study area could be achieved through efficient extension programs about advanced scientific management practices for optimum utilization of resources.

Viral co-infections of the porcine respiratory tract: Insight into the local cytokine response

Infections of the porcine respiratory tract are frequently multifactorial, with more than one pathogen involved. They have a significant impact on the efficiency of pig production. One example of such a mixed infection is the porcine respiratory disease complex (PRDC). PRDC can be caused by various viral or bacterial agents. The main viral agents associated with PRDC and considered the primary pathogens are porcine reproductive and respiratory syndrome virus (PRRSV), swine influenza virus (SIV), and porcine circovirus type 2 (PCV2). PRRSV, SIV, and PCV2 are known as inducers of inflammatory cytokines. Cytokines play an important role in all aspects of immune responses, but their uncontrolled release in virus-induced diseases may aggravate the course of the disease and the severity of pathological lesions. Although data regarding the kinetics of the local cytokine response in porcine lungs during mono-infection with these pathogens are abundant, their impact on each other during simultaneous infection in different combinations is not thoroughly understood. This paper aims to present the available data on interactions between SIV, PRRSV, and PCV2 in mixed infections of the porcine respiratory tract and the influence of co-infections on local cytokine profiles in the lungs.

Impact of Animal Welfare on the Efficiency of Pig Production in Korea

The purpose of this study is to analyse the effect of factors related to animal welfare on pig productivity in Korea. For the normal DEA and bootstrap DEA analysis, MSY was selected as the output variable and 6 items of animal welfare was selected as the input variables. As a result, the production efficiency of CCR model was 0.8613 for DEA and 0.8256 for bootstrap DEA, and the production efficiency of BCC model was 0.9140 for DEA and 0.8845 for bootstrap DEA. And the scale efficiency showed an overall average of 0.9425 for DEA (0.9354 for bootstrap DEA). In the OLS regression analysis, anmimal welfare point as an input factor had negative coefficient. In conclusion, animal welfare efforts have had little effect on MSY in hog farms in Korea yet.

Feeding Strategies to Reduce Nutrient Losses and Improve the Sustainability of Growing Pigs.

The efficiency of pig production using nutrients has increased over the years. Still, better efficiency of nutrient utilization can be achieved by feeding pigs with diets adjusted to their estimated requirements. An increase in nutrient efficiency of utilization represents economic gains while maximizing environmental performance. The objective of this paper is to review the impact of different methods of diet formulation that provide farm animals with the amount of nutrients to satisfy their needs while minimizing nutrient excretion and greenhouse gas emissions. Diet formulation is one tool that can help to maximize nitrogen and energy utilization by decreasing crude protein content in diets. The use of local feedstuff and non-human-edible products (e.g., canola meal) associated with synthetic amino acid inclusion in the diet are valuable techniques to reduce carbon footprint. Precision feeding and nutrition is another powerful tool that allows not only daily tailoring of diets for maximal nutrient efficiency of utilization but also to reduce costs and improve nitrogen efficiency of utilization. In this review, we simulated through mathematical models the nitrogen and energy efficiency of utilization resulting from crude protein reduction in the diet. An 8% crude protein reduction in the diet can increase nitrogen efficiency of utilization by 54% while costing 11% less than a control diet without synthetic amino acids. The same reduction in crude protein represented a major improvement in available energy due to the decrease of energetic losses linked to protein deamination. Urinary and hindgut fermentation energy losses were 24% lower for pigs fed with low-protein diets when compared to control diets. In terms of modern feeding techniques and strategies, precision feeding and nutrition can decrease nitrogen excretion by 30% when compared to group phase feeding. The benefits of feeding pigs with low-protein diets and precision feeding techniques are additive and might result in a 61% nitrogen efficiency of utilization. There is room for improvement in the way nutrient requirements are estimated in pigs. Improving the understanding of the variation of nutrient utilization among pigs can contribute to further environmental gains.