Efficacy Of Various Concentrations Research Articles

Development of an Advanced Material for Obturation in Primary Teeth Based on the Microbiological Analysis of the Antimicrobial Efficacy of Various Concentrations: An In Vitro Study.

Endodontics in primary teeth has brought a revolution in preserving the primary teeth until it is physiological exfoliation. However, reinfection and failure of the pulpectomy-treated teeth should not be neglected. This raises the need for the best root canal filling material with the strongest antibacterial capabilities for primary teeth. The current study set out to assess the antimicrobial efficacy of different concentrations of the novel obturating material against Streptococcus mutans and Enterococcus faecalis, the two most common microorganisms found in primary tooth infections. The antimicrobial efficacy of the novel obturating material was evaluated against Streptococcus mutans and Enterococcus faecalis using Mueller-Hinton agar (MHA) for 24 hours at 37°C. Twelve distinct concentrations of the material were prepared by varying the proportions of calcium hydroxide, zinc oxide, and metronidazole. After a day, the zone of inhibition was measured in millimeters. After entering the input into a Microsoft Excel spreadsheet, the values underwent statistical analysis. The mean zone of inhibition against Streptococcus mutans and Enterococcus faecalis was greater with 2% 60-40 concentration of the novel obturating material. However, no statistically significant difference was noted (p = 0.74, 0.22, respectively). The most effective formulation of the novel obturating material with the greatest antimicrobial property was determined to be 2% 60-40 concentration of metronidazole, calcium hydroxide, and zinc oxide. Govindaraju L, Jeevanandan G. Development of an Advanced Material for Obturation in Primary Teeth Based on the Microbiological Analysis of the Antimicrobial Efficacy of Various Concentrations: An In Vitro Study. Int J Clin Pediatr Dent 2023;16(6):800-803.

Clinical Efficacy of Different Concentrations of Povidone Iodine in the Management of Peri-Implant Mucositis.

Mechanical plaque control is the first line of management of peri-implant diseases. Povidone iodine is one of the broad spectrum and potent antiseptics available at various concentrations. The aim of the study was to assess and compare the efficacy of various concentrations of povidone iodine in the management of peri-implant mucositis. In the present double blinded, parallel designed, randomized clinical trial, a total of 60 patients with peri-implant mucositis (20 participants in each group [Group 1 (povidone iodine 0.1%), Group 2 (povidone iodine 2%) and Group 3 (povidone iodine 010%)] were enrolled. Scaling and root planing was done and then the peri-implant sulcus was irrigated with respective irrigant and repeated once in a week for 4 weeks. Loe and Silness Gingival Index (GI) and Mombelli Modified sulcular bleeding index (BI) were recorded at baseline and after a month and compared. Statistical analysis was done using One-way ANOVA and Tukey's HSD post hoc test. A statistically significant difference (P = 0.000) observed between the three concentrations when compared after 1 month. Also, a statistically significant difference between Group 1 and Group 3 and Group 2 and Group 3 was observed in terms of post GI (P = 0.000) and post BI (P = 0.000) but statistically no significant difference was observed between Group 1 and Group 2 in terms of post GI (P = 0.171) and post BI (P = 0.338). The 2% and 10% povidone iodine showed significant improvement in gingival index and bleeding index and hence it could be an effective adjunct to scaling and root planing in the management of peri-implant mucositis.

Determination of the efficacy of thymol, Artemisia absinthium oil and nanoparticle ozone in the treatment of Nosema ceranae in adult honey bees

The therapeutic efficacy of various concentrations and combinations of thymol, Artemisia absinthium oil and nanoparticle ozone against Nosemosis caused by Nosema ceranae in honey bees was studied. Two-hundred colonies were examined for N. ceranae positivity and negativity under light microscopy; 119 colonies were positive and 81 colonies were negative for Nosemosis. The study evaluated the therapeutic efficacy of 17 different combinations against N. ceranae. Nine of the 17 combinations were applied by spray to frames and eight were applied orally (combinations added to the sugar syrup reservoir) to the hives. Each of the 17 combinations was prepared in 1,000 ml from (2%, 3% and 5%) of thymol and (2% and 3%) A. absinthium oil, and 1,000, 2,000, 4,000, and 8,000 ppm concentrations of nanoparticle ozone. N. ceranae spore counts before and after the treatment were counted on Neubauer thoma slides by the digestion method, and the efficacy of the combinations was determined by the percent reduction test. In spray form, 200 ml 2,000 ppm nanoparticle ozone + 100 ml 3% thymol + 700 ml sugar syrup (efficacy-89.47%) (Group 5) and in oral form, 250 ml 2% thymol + 200 ml 2% A. absinthium oil + 500 ml sugar syrup (efficacy-85.95%) (Group 16), were found to be more effective. In conclusion, the combination of thymol and nanoparticle ozone in spray form was more effective than other combinations in this study.

Comparative efficacy of different concentrations of chlorhexidine for prevention of ventilator‐associated pneumonia in intensive care units: A systematic review and network meta‐analysis

AbstractBackgroundVentilator‐associated pneumonia is a common type of hospital‐acquired infection in critically ill patients. The implementation of effective oral care with chlorhexidine is known to be an effective intervention to prevent ventilator‐associated pneumonia. Nonetheless, no well‐supported evidence exists yet to rank the efficacy of various concentrations of chlorhexidine.AimTo compare the efficacy of different concentrations of chlorhexidine (0.12%, 0.20% and 2.0%) for prevention of ventilator‐associated pneumonia.Study DesignElectronic databases, as well as bibliographies, were systematically searched to find relevant studies published in English from inception until January 1, 2022. We included randomized controlled trials of mechanically ventilated adult patients in intensive care settings comparing at least two of the following treatments: 0.12% chlorhexidine, 0.2% chlorhexidine, 2.0% chlorhexidine, or placebo. The selected outcome was the incidence of ventilator‐associated pneumonia. A frequentist network meta‐analysis was performed. We ranked the concentrations of chlorhexidine for the selected outcome by p score (estimate of the probability of being the best treatment).ResultsWe included 19 trials with 3183 participants, conducted across nine countries. The use of 2.0% chlorhexidine and 0.12% chlorhexidine decreased the risk of ventilator‐associated pneumonia compared with placebo (OR 0.46, 95% CI = 0.28–0.75; OR 0.49, 95% CI = 0.33–0.71, respectively), whereas the effect resulting from 0.02% chlorhexidine remains unclear (OR 0.78, 95% CI = 0.54–1.13). Our network meta‐analysis ranked the 2.0% concentration as the most beneficial for ventilator‐associated pneumonia prevention (p score: 0.92).ConclusionThe 2.0% chlorhexidine and the 0.12% chlorhexidine reduce the risk of ventilator‐associated pneumonia in mechanically ventilated patients. According to the results of our network meta‐analysis, 2.0% chlorhexidine seems more beneficial. Chlorhexidine needs to be further evaluated in large‐scale controlled trials.Relevance to clinical practiceIn patients in intensive care units, and within the inevitable limitations of a network meta‐analysis, this study proposes that 2.0% chlorhexidine is superior to other chlorhexidine concentrations in terms of preventing ventilator‐associated pneumonia.

Efficacy of various concentrations of synthetic hormones on the induced breeding of Channa marulius (Sole)

AbstractA study was conducted to determine the artificial breeding response, embryonic and larval development of giant snakehead, Channa marulius, with the application of various dosages of stimulatory hormones: Suprefact® (LHRH) agonist and Ovaprim® (GnRH + dopamine inhibitor). First and second hormonal dosages of Suprefact® (diluted) and Ovaprim® (0.3, 0.4, and 0.5 ml for male and 0.8, 0.9, and 1.0 ml for females per kilogram body weight) were used. There were three treatments T1, T2, and T3 and each treatment had three replicates. Male fish were treated with T1 (0.3), T2 (0.4), T3 (0.5) ml kg−1 of body weight while female fish with T1 (0.8), T2 (0.9), T3 (1.0) ml kg−1 of body weight. The results showed that fish stimulated with T3 (1.0) obtained better fecundity rate (2951.7) followed by T2 (0.4) (2678.3), while the lowest fecundity (466.7) was recorded in T1. The numerically higher values of gonado‐somatic index (males and females) and fertilization rate appeared in T2. The highest survival rate (97.1%) was found in T3 followed by T2 (97.0%), while the lowest (32.2%) in T1. In conclusion, the application of Suprefact® and Ovaprim® at 0.4 and 0.5 ml kg−1 body weight for male and 0.9 and 1.0 ml kg−1 for female fish successfully stimulated snakehead fish induced breeding.

Trichoderma virens mitigates the root-knot disease progression in the chickpea plant

ABSTRACT This study was planned to investigate the efficacy of various concentrations of Trichoderma virens against Meloidogyne incognita in vitro. The five concentrations viz., S, S/2, S/ 10, S/25, S/50 were prepared and planned for in vitro study to test the potential of T. virens against hatching and mortality of second-staged juveniles of M. incognita. It was observed a reduction in second-staged juveniles hatching within all tested aqueous concentrations of T. virens. The second-stage juvenile mortality was also recorded in the above-given concentrations of T. virens. The maximum decrease in second-stage juveniles hatching was found in standard aqueous fungal concentration (S). Moreover, in the same T. virens concentration (S), mortality of juveniles was also recorded as highest, and was followed by S/2, S/10, S/25 and S/50. Additionally, the application of T. virens as an individual, simultaneous, and sequential order with M. incognita was also investigated in pot-grown chickpea plants and found that its use was significantly effective in suppressing root-galling disease and improved the plants' growth and physiological attributes. According to the correlation coefficient analysis, the root-knot index correlated significantly with the per cent reduction of the plants' growth and physiological attributes.

Biosynthesis and characterization of zinc oxide nanoparticles from an estuarine-associated actinobacterium Streptomyces spp. and its biotherapeutic applications

The present study was conducted to biosynthesize zinc oxide nanoparticles (ZnONPs) by an estuarine associated actinobacterium Streptomyces spp., characterized its spectral features and also determined its biomedical properties. At first, ZnONPs were synthesized by actinobacterial-mediated reduction of zinc oxide metal and then, it was characterized by following standard methodologies. The biosynthesized ZnONPs were confirmed through the reduction of Zn ions with the strong intensity range (3eV) at zinc region and the absorption range of 363nm. The XRD pattern of biosynthesized ZnONPs showed six intensive peaks with 2θ values of 36.38-83.34º. This ZnONPs exhibited spherical in shape with the average size ranges in between 51.42 and 65.13nm. The antimicrobial efficacy of various concentrations of ZnONPs was determined against both bacterial and fungal pathogens and it revealed the highest growth inhibition against E. coli (22mm) at 20μl concentration with the respective MIC and MBC values of 0.625μg/ml and ≤ 2.5μg/ml. Similarly, the antifungal activity represented the maximum (20mm) zone of growth inhibition against Candida fructus at 20μl concentration. Furthermore, the in vitro antioxidant, anti-inflammatory and antibiofilm efficiency of ZnONPs were tested. The result inferred that the maximum in vitro total antioxidant activity (70.86%), DPPH (79.26%), nitric oxide (67.31%) and hydroxyl radical (76.83%) scavenging activities were noted at 125μg/ml concentration of ZnONPs with the respective half maximal inhibitory concentration (IC50) values of 81.69, 83.42, 76.09 and 82.46μg/ml. In in vitro anti-inflammatory activity study, the maximum concentration (500μg/ml) of ZnONPs showed the inhibition of 94.62% with the IC50 value of 146.70μg/ml. Finally, the ZnONPs effectively reduced the biofilm formation with the highest percentage (91.08%) of inhibition against E. coli at 75μg/ml concentration. Based on these results, it could be concluded that the actinobacterium Streptomyces spp., mediated biosynthesized ZnONPs has biomedical potential with antimicrobial, antioxidant, antiinflammatory and antibiofilm properties.

Efficacy of some Egyptian native plant extracts against\xa0Haemonchuscontortus in vitro and in experimentally infected sheep along with the associated haematological and biochemical alterations

BackgroundHaemonchosis is a serious disease affecting ruminants’ productivity worldwide. Medicinal plants are deemed one of the most natural bio-products safely used as alternatives to the synthetic anthelmintics. In the present study, comparative efficacy of crude ethanolic extracts (CEEs) of Artemisia herba-alba (A. herba-alba), Balanites aegyptiaca (B. aegyptiaca) and Allium sativum (A. sativum) as alternative treatments was tested on Haemonchus contortus ( H. contortus). An in vitro test to evaluate the anthelmintic efficacy of various concentrations of extracts at 25, 30 and 50 mg/ml was accomplished on motility and viability of adult worms in comparison with albendazole, reference drug at 10 μg/ml at various time intervals. An in vivo test was carried out in lambs experimentally infected with H. contortus to detect anthelmintic activity of CEEs of A. herba-alba and B. aegyptiaca compared to albendazole. Fifteen parasite-free Baladi Egyptian lambs aged 4–8 months old were categorized into five groups, each of three lambs as follows: G1 was kept as uninfected untreated one, G2 was utilized as infected untreated group, G3 was given CEE of A. herba-alba, G4 was received CEE of B. aegyptiaca, and G 5 was treated with albendazole.ResultsThe in vitro test revealed that CEE of B. aegyptiaca had the most significant anthelmintic activity on adult H. contortus followed by A. herba-alba, while A. sativum was of the lowest effect. The in vivo test showed that the CEE of B. aegyptiaca achieved an excellent faecal egg reduction (100%) at the 7th day post-treatment. The most efficient treatments that improved the haematological parameters and regained the level of serum total protein, albumin and A/G ratio, serum globulin, SGoT, SGPT, urea and creatinine to the almost normal levels were CEE of B. aegyptiaca, albendazole and CEE of A. herba-alba, respectively.ConclusionsThis study highlighted the marked anthelmintic potency of the CEEs of B. aegyptiaca and A. herba-alba on H. contortus and the superiority of CEE of B. aegyptiaca as a talented anti-parasitic medicinal plant for sheep.

Probiotic Ayran development by incorporation of phytosterols and microencapsulated Lactobacillus casei L26 in sodium caseinate–gellan mixture

Ayran is a traditional fermented dairy drink that is manufactured by mixing yoghurt with water. In this research, the development of phytosterol‐enriched probiotic Ayran, the efficacy of various concentrations of phytosterol (0, 0.5 and 1 mg/L) and inoculation type (free or encapsulated) were investigated. The viability of Lactobacillus casei L26 and physicochemical indices of samples during storage time was studied. Samples with encapsulated probiotics showed lower serum separation due to the hydration of encapsulated agents. Sensory scores revealed that phytosterols are suitable for the enrichment of the probiotic Ayran. Moreover, phytosterol showed no significant changes in pH, acidity and viscosity during storage.

Development of a porcine hard-to-heal wound model: evaluation of a bromelain-based enzymatic debriding agent.

We describe the development of a novel porcine eschar model and compare the debridement efficacy of various concentrations of a novel bromelain-based enzymatic agent with collagenase. Full thickness excisional wounds were created on pigs and injected intradermally with various doses of doxorubicin. Wounds were monitored for a period of 46 days for the development of eschar and wound closure. After determining the optimal concentration and dose of doxorubicin resulting in non-healing eschars, these conditions were used to create additional wounds on another set of animals. The resulting eschars were treated with various concentrations of a novel bromelain-based enzymatic agent (EscharEx-02) or collagenase. The primary endpoint was greater than 95% removal of the central eschar. Consistent eschars composed of two distinct areas (a central area of exudate and slough representing the hard-to-heal wound bed, and a peripheral area of full-thickness mummified necrosis) were seen after injection of doxorubicin (0.5 ml/cm2 of stock solution 0.75mg/ml) at one and six days after wound creation. Complete removal of the central eschar was achieved in all wounds after five and eight treatments with 5% and 2% EscharEx-02 respectively. Complete removal of the central eschar with collagenase was achieved in 0% and 82% of the wounds after 10 and 16 treatments respectively. We describe a porcine model for creating eschars similar to hard-to-heal wounds in humans. A novel bromelain-based enzymatic debridement agent was more effective than a commercially available collagenase in removing eschars in this wound model.

Определение терапевтической эффективности различных концентраций сивелестата на лабораторной модели псориаза (Часть 1)

Определение терапевтической эффективности различных концентраций сивелестата на лабораторной модели псориаза (Часть 1)

Efficacy of atropine eyedrops in reducing myopia progression and axial elongation in myopic children: a meta-analysis

Purpose: To determine the efficacy of various concentrations of atropine eyedrops on retarding myopia progression and axial elongation in Asian children. Study design: Meta-analysis. Methods: Randomized clinical trials and prospective interventional non-randomized studies which enrolled children aged 4 to 14 years old who received atropine treatment for myopia were included in the study. The Cochrane Collaboration 6 aspects of bias was used to assess the risk of bias for all included studies. Outcome measures were myopia progression and axial elongation. Meta-analysis was conducted using the random-effects model. Results: Eight randomized clinical trials and two prospective interventional non-randomized studies which included a total of 1,229 Asian children were included in the analysis. The pooled mean difference between control and atropine for myopia progression was 0.77 diopters (D) per year [CI 0.64, 0.89]. Subgroup analysis by concentration showed a decreasing trend with decreasing concentration. The pooled mean difference of myopia progression for 1%, 0.5%, 0.25%, and 0.1–0.125% atropine was 0.97 D/year [CI 0.72, 1.21], 0.88 D/year [CI 0.74, 1.02], 0.79 D/year [CI 0.37, 1.21], and 0.80 D/year [CI 0.62, 0.97], respectively; whereas that for 0.01% atropine was 0.46 D/year [CI -0.02, 0.94] indicating that this intervention may or may not be favorable for slowing myopia progression. The pooled mean difference between control and atropine for axial elongation was -0.22 mm [CI -0.29, -0.14] favoring atropine. Subgroup analysis by concentration also showed decreasing trend with decreasing concentration. The pooled mean difference of axial elongation for 1%, 0.5%, 0.1%, 0.05%, and 0.025% atropine was -0.44 mm [CI -0.57, -0.32], -0.19 mm [CI -0.35, -0.04], -0.10 mm [CI -0.17, -0.03], -0.21 mm [CI -0.28, -0.14], and -0.12 mm [CI -0.16, -0.08], respectively; whereas that for 0.01% atropine was -0.01 mm [CI -0.09, 0.06] indicating that this intervention may or may not be favorable in reducing axial elongation. Conclusion: This meta-analysis shows that the effects of atropine for both myopia progression and axial elongation are dose-dependent for the concentration 0.025% to 1%. Results for 0.01% atropine are still equivocal.

Cevimeline-induced anti-inflammatory effect through upregulations of mucins in the ocular surface of a dry eye mouse model

This study aimed to investigate the effects of various concentrations of cevimelines (CVMs) and compare them with commercial drugs in a murine model of dry eye. The experimental mouse model used male and female NOD.B10.H2b mice over 12 weeks of age. Desiccation stress was performed at 30–40% ambient humidity, and subcutaneous injection of 0.5 mg/0.2 mL scopolamine hydrobromide was performed four times a day for 10 days. The efficacy of various concentrations of CVMs (seven experimental groups) was first evaluated, and then 2% CVM was compared with commercial drugs, such as cyclosporine A (CsA), diquafosol (DQS), and rebamipide (REB) (seven experimental groups). The clinical changes, including tear production, corneal irregularity, and fluorescein staining, were measured after the instillation of various concentrations of CVMs and commercial drugs for 0, 3, 5, 7, and 10 days. Histological changes, such as corneal detachment, conjunctival goblet cell and mucin density staining, were assessed by staining the cornea or conjunctiva with hematoxylin-eosin, periodic acid-Schiff, and alcian blue. The expression of inflammatory markers and mucin factors was detected by immunohistochemistry and immunofluorescence in the lacrimal gland, cornea, and conjunctiva. Tear production was significantly increased in the 2% CVM group and was similar to that in the DQS and REB groups (P < 0.05). The corneal smoothness and fluorescein staining score were significantly improved in the 2% CVM group and were similar to those in the REB group (P < 0.05). Corneal epithelial cells were significantly decreased in the 2% CVM group, with similar observations made in the DQS and REB groups (P < 0.05). The conjunctival goblet cells and mucin density recovered in the 2% CVM group were similar to those in the CsA and REB groups (P < 0.05). The 2% CVM group showed suppressed expression of inflammatory factors in the lacrimal gland and was comparable to that seen in the CsA and REB groups. The expression of mucin factors was upregulated in the cornea and conjunctiva of the 2% CVM group and was similar to that of the CsA and REB groups. In conclusion, administration of CVM resulted in recovery or clinical and histological improvement of the murine dry eye model, and all the observed parameters were comparable to those with commercial drugs.

Effect of Chlorine Dioxide Treatment on Human Pathogens on Iceberg Lettuce.

In the vegetable processing industry, the application of chlorine dioxide (ClO2) as a disinfectant solved in washing water to eliminate undesirable microorganisms harmful to consumers’ health and the shelf life of produce has been discussed for years. The disinfection efficacy depends on various factors, e.g., the location of microorganisms and the organic load of the washing water. The present study analyzed the sanitation efficacy of various concentrations of water-solved ClO2 (cClO2: 20 and 30 mg L−1) on Escherichia coli (1.1 × 104 cfu mL−1), Salmonella enterica (2.0 × 104 cfu mL−1) and Listeria monocytogenes (1.7 × 105 cfu mL−1) loads, located on the leaf surface of iceberg lettuce assigned for fresh-cut salads. In addition, it examined the potential of ClO2 to prevent the cross-contamination of these microbes in lettuce washing water containing a chemical oxygen demand (COD) content of 350 mg L−1 after practice-relevant washing times of 1 and 2 min. On iceberg leaves, washing with 30 mg L−1 ClO2 pronouncedly (1 log) reduced loads of E. coli and S. enterica, while it only insignificantly (<0.5 × log) diminished the loads of L. monocytogenes, irrespective of the ClO2 concentration used. Although the sanitation efficacy of ClO2 washing was only limited, the addition of ClO2 to the washing water avoided cross-contamination even at high organic loads. Thus, the application of ClO2 to the lettuce washing water can improve product quality and consumer safety.

The Skin Whitening Effect of Co-Cultured Conditioned Medium: Involvement of Synergy between Stem Cells and Immune Cells

Many researchers have described that mesenchymal stem cells conditioned medium and immune cells conditioned medium have a clear whitening effect when they are used as cosmetic ingredients. In this study, we confirmed the whitening efficacy of various concentrations of immune cells and stem cell conditioned media. The author tried to study a conditioned medium that has a strong whitening effect even with a composition of less than 20% (the most used concentration in cosmetics). Because of the fact that the conditioned medium contains various cytokines and growth factors secreted by stem cells or immune cells, it is known to have effects such as wound healing, antioxidant, and whitening effect. Recently, stem cells have been used not only in the development of cosmetic raw materials but also in skincare procedures, and there are reports being released of cosmetics using immune cells conditioned medium. The concentration-dependent whitening effect equivalently increased as the concentration of the mono-cultured conditioned medium was obtained through the stem cells or immune cells culture. In the case of co-culture, whitening results are like the effect of positive control such as arbutin in the medium carrying only 10% of the co-cultured conditioned medium. It is possible that enhanced whitening efficiency in co-cultured conditioned medium leads to a major innovation in the global cosmetic market.

Ethanol and isopropanol inactivation of human coronavirus on hard surfaces

BackgroundThe coronavirus disease 2019 pandemic has greatly increased the frequency of disinfecting surfaces in public places, causing a strain on the ability to obtain disinfectant solutions. An alternative is to use plain alcohols (EtOH and IPA) or sodium hypochlorite (SH).AimTo determine the efficacy of various concentrations of EtOH, IPA and SH on a human coronavirus (HCoV) dried on to surfaces using short contact times.MethodsHigh concentrations of infectious HCoV were dried on to porcelain and ceramic tiles, then treated with various concentrations of the alcohols for contact times of 15 s, 30 s and 1 min. Three concentrations of SH were also tested. Reductions in titres were measured using the tissue culture infectious dose 50 assay.FindingsConcentrations of EtOH and IPA from 62% to 80% were very efficient at inactivating high concentrations of HCoV dried on to tile surfaces, even with a 15-s contact time. Concentrations of 95% dehydrated the virus, allowing infectious virus to survive. The dilutions of SH recommended by the Centers for Disease Control and Prevention (1/10 and 1/50) were efficient at inactivating high concentrations of HCoV dried on to tile surfaces, whereas a 1/100 dilution had substantially lower activity.ConclusionsMultiple concentrations of EtOH, IPA and SH efficiently inactivated infectious HCoV on hard surfaces, typical of those found in public places. Often no remaining infectious HCoV could be detected.

Efficacy of Various Concentrations of Honey Solution in the Fixation of Some Selected Tissues in Wistar Rats

Introduction: Honey is thought to preserve the cells by preventing autolysis and putrefaction. Thus, studies have shown that honey has a good preventive putrefactive property mainly because honey contains seven tetracycline derivatives, fatty acids, lipids, amylases and ascorbic acid and hydrogen peroxide. Therefore, honey is used as an agent for preventing autolysis and putrefaction.&#x0D; Aim: This research aims to investigate the efficacy of some concentrations of the honey solution in fixation of some tissues (Such as kidney, liver, heart, lungs and muscle).&#x0D; Methodology: A Wistar rat was sacrificed by vertical abdominal incision and the above-mentioned organs ware fixed in 10% formalin (positive control), 10% honey and 20% honey this grouped the research specimen into 3 groups. Histological examinations of these organs were carried out. &#x0D; Results: Almost all the histological sections fixed in honey solution revealed normal nuclear as well as a cytoplasmic outline as compared with formalin-fixed organs, except for muscle which shown some level of swollen and fragmenting tissue.

Innovative Delivery of Cu(II) Ions by a Nanostructured Hydroxyapatite: Potential Application in Planta to Enhance the Sustainable Control of Plasmopara viticola.

Downy mildew caused by Plasmopara viticola is probably the most serious disease affecting grapevine (Vitis vinifera), and it is capable of causing consistent yield losses. In organic viticulture, the only acceptable and effective means to control the disease is by applications of copper-based fungicides. However, the use of copper in agriculture is expected to be further restricted by European countries because of its critical ecotoxicological and phytotoxicological profile. Research on ways to reduce the effective amounts of copper by developing innovative formulations as well as optimization of the distribution and persistence of copper-based pesticides for downy mildew control seems to be a promising approach. This research investigated the delivery properties of biomimetic synthetic hydroxyapatite (HA) to enhance the biological activity of Cu(II) ions. To this aim, four Cu(II) compounds were formulated with the innovative HA component and applied in an in vitro antifungal assay against Botrytis cinerea, a common grapevine pathogen suitable for in vitro activity tests, and finally, in in planta efficacy assays against P. viticola under greenhouse conditions. The in vitro results highlighted a different inhibition activity for each Cu(II) compound and indicated a different interaction between the cupric compounds and HA, potentially related to different delivery mechanisms of Cu(II) from HA. Under greenhouse conditions, additional findings on the biological activity of the applied formulations were gained, especially on the efficacy of various concentrations of HA in the formulations, the influence of dose variation of the formulation and the treatment efficiency, and the persistence under rain-washing effect. This study revealed promising findings on the formulation based on the HA particles and the soluble Cu(II) compound, which resulted in reduced disease severity and incidence in all of the experimental conditions, including the lower Cu(II) dosage and the rain-washing effect. This suggests that coformulation of the three insoluble Cu(II) compounds with HA might significantly enhance the adsorption and release of Cu(II) ions by HA particles.

An In Vitro Evaluation of Antibacterial Efficacy of Various Concentration of Eucalyptus globulus Leaf Extract on Periodontal Pathogens

The aim of the present study was to evaluate the antibacterial efficacy of various concentrations of Eucalyptus globulus leaf extract on periodontal pathogens. Matured, healthy and disease-free leaves of Eucalyptus globulus were collected. The leaves were washed under tap water and finally dried in an oven at a temperature of 45°C for 48 hours. The dried plants were ground in an electric blender to make them into a powder. The powder was mixed with 100% ethanol and kept it inside a shaker overnight at 35°C. The mixture was centrifuged for 10 minutes at 2,500 rpm. Three different concentrations (10%, 50%, and 100% v/v) were used as antibacterial agents. Chlorhexidine (0.2%) was considered as positive control and dimethyl formamide was considered as negative control against P. gingivalis and A. actinomycetemcomitans. The disc diffusion method was used to determine the extract's antibacterial activity against the test organisms. A digital Vernier caliper was used to measure the diameter of antibacterial activity showing the zone of inhibition in millimeters. Eucalyptus globulus with 100% concentration showed a maximum zone of inhibition against A. actinomycetemcomitans and P. gingivalis (5.38 ± 0.32 mm, 4.82 ± 0.11 mm) followed by 50% and 10% accordingly. The negative control of dimethyl formamide showed a zone of inhibition of 0.48 ± 0.96 mm and 0.63 ± 0.20 mm against A. actinomycetemcomitans and P. gingivalis. The positive control of 0.2% chlorhexidine showed a zone of inhibition of 8.46 ± 1.02 mm and 7.18 ± 0.54 mm against A. actinomycetemcomitans and P. gingivalis. The ANOVA test showed a highly significant antibacterial efficacy in 0.2% chlorhexidine and 100% concentration Eucalyptus globulus. A significant maximum zone of inhibition against A. actinomycetemcomitans and P. gingivalis was showed by 100% concentration of Eucalyptus globulus. Other than the systemic diseases treatment, Eucalyptus globulus also serves as an effective promising alternative to antibiotics in the prevention of oral infections because of the natural phytochemicals existing in them.

Evaluation of efficacy on RANKL induced osteoclast from RAW264.7 cells.

Established RAW264.7 cell lines for osteoclastic differentiation has been widely engaged in bone homeostasis research, however, the efficacy of RANKL independently stimulating has rarely been defined, because protocols were usually developed and modified by various laboratories. Otherwise, problematic issues are also lie in the cell's seeding density, RANKL stimulating time point, and distinguishing osteoclastogenesis ability of RANKL-treated RAW264.7 cells. Therefore, in the current study, we examined the efficacy of various concentrations of RANKL-treated RAW264.7 for its osteoclastic differentiation with or without pretreated other costimulators such as: LPS and/or M-CSF. The oteoclastogenesis ability of RANKL-treated RAW264.7 cells was demonstrated by bone resorption pit, F-actin, and osteoclastogenesis specific marker studies. Besides that, through tartrate-resistant acid phosphatase (TRAP) staining, we clarified to start the treatment with 30 ng/ml RANKL at 12 hr after seeded RAW264.7 with the density of 6.25 × 10 3 cells/cm 2 manifested an significantly increased number of multinucleated osteoclastic cells. Overall, our results establishing an optimal method for RANKL independently inducing RAW 264.7 cell osteoclastic differentiation, which could efficiently generate osteoclasts in vitro for significant advances in our understanding of bone biology.