Efficacy Of Solid Lipid Nanoparticles Research Articles

High anticancer efficacy of solid lipid nanoparticles containing Zataria multiflora essential oil against breast cancer and melanoma cell lines

BackgroundThe cancer burden is rising rapidly worldwide, and it annually causes about 8.8 million deaths worldwide. Due to chemical drugs’ side effects and the emergence of resistance, the development of new green drugs has received much attention. We aimed to investigate whether solid-lipid nanoparticles containing essential oil of Zataria multiflora (ZMSLN) enhanced the anticancer efficacy of the essential oil against breast cancer (MDA-MB-468) and melanoma (A-375) cells.ResultsZMSLN was prepared by the high-pressure homogenizer method; particle size 176 ± 8 nm, polydispersity index 0.22 ± 0.1, entrapment efficiency 67 ± 5%. The essential oil showed a dose-dependent antiproliferative effect on MDA-MB-468 and A-375 cells at all examined concentrations (75, 150, 300, 600, and 1200 μg/mL). Interestingly, after treating both cells with 75 μg/mL of ZMSLN, their viabilities were reduced to under 13%.ConclusionThe finding showed that ZMSLN had a distinct antiproliferative efficacy; it could thus be considered a green anticancer candidate for further in vivo and in vivo studies.

The effect of efflux pump inhibitors on in vitro and in vivo efficacy of solid lipid nanoparticles containing SN38

7-ethyl-10-hydroxy camptothecin (SN38) is poorly soluble in water and pharmaceutical solvents. Oral administration of SN38 was restricted due to poor solubility. Many ways such as encapsulation in carriers are used for solving this restriction. Solid lipid nanoparticles (SLNs) are able to encapsulate lipophilic drugs and improve oral bioavailability. In addition, concomitant use of the efflux pump inhibitors can improve the performance of the encapsulated drug. In the present study, SN38 was loaded in SLNs. Nanoparticle size, zeta potential and drug encapsulation efficacy were investigated. The drug release from nanoparticles was also determined in the simulated intestinal fluid (SIF) and simulated gastric fluid (SGF). Cytotoxicity of nanoparticles after 24 and 48 h were measured in C26 and Caco-2 cell lines. The cellular uptake of the drug was determined. Tumor growth inhibition and survival were evaluated in animal model. The results showed that the drug-loaded SLNs were about 172.3 ± 3.3 nm in size. The encapsulation efficacy was 61% ± 7. In cellular studies, higher toxicity of the drug-loaded nanoparticles with the two substances piperine and quercetin were achieved in the Caco-2 cell line. It was observed that drug uptake of nanoparticles containing SN38 with or without piperine and quercetin by C26 and Caco-2 cell line was significantly higher than free SN38 in 2 and 4 h. According to in vivo studies, the use of piperine and quercetin with SLN containing SN38 has produced a favorable synergistic effect as increasing the survival time in animal model.

Designed Meso‐macroporous Silica Framework Impregnated with Copper Oxide Nanoparticles for Enhanced Catalytic Performance

AbstractThe template efficacy of solid lipid nanoparticles for generating porous silica materials with the amalgamation of Cu‐functionalized cetylpyridinium chloride (CPC; as a co‐emulsifier and as a metal source for generation of CuO oxide nanoparticles) has been explored. Impregnation of CuO nanoparticles (∼10–12 nm) onto the silica matrix proffers to be a propitious route for the fabrication of twin sized porous, highly active catalytic materials. The surface morphology and structural characterization of the synthesized CuO@meso‐macroporous silica framework was elucidated by TEM, field ‐emission (FE)‐SEM, energy‐dispersive X‐ray (EDX), wavelength dispersive (WD)‐XRF, X‐ray photoelectron spectroscopy (XPS), small‐angle X‐ray scattering (SAXS), XRD, and N2 adsorption/desorption studies. All the results have shown that there exists a structural network interconnecting the mesopores with macropores. The catalytic performance of the synthesized framework (ascribed catalyst) was checked for two model reactions. For the reduction of p‐nitrophenol to p‐aminophenol, only 0.25 % (w/v) catalyst was sufficient to accomplish the reaction in 6 min, giving 99.9 % yield. On the other hand, the redox reaction of potassium hexacyanoferrate(III) and sodium thiosulfate utilized 5 % (w/v) catalyst and completed the reaction in 40 min, yielding 98.43 %. Therefore, CuO@meso‐macroporous silica materials could hold great importance in the world of nanocatalysis owing to the present promising results.

Development of Piroxicam loaded SLN-based Hydrogel for Transdermal Delivery

The aim of present study was to construct and investigate the efficacy of solid lipid nanoparticles (SLNs) based hydrogel for transdermal delivery of non-steroidal anti-inflammatory drug (NSAID) piroxicam. Solid lipid nanoparticles (SLNs) of piroxicam were produced by solvent emulsification diffusion method in a solvent saturated system. The SLNs were composed of tripalmitin lipid, polyvinyl alcohol (PVA) as stabilizer, and solvent ethyl acetate. All the formulations were subjected to particle size analysis, zeta potential, drug entrapment efficiency, percent drug loading determination and in-vitro release studies. The SLNs formed were in nano-size range with maximum entrapment efficiency of 88.50±0.92. Further, Carbopol-934 was used as a gel matrix for preparation of hydrogel for improving the penetration rate across the skin and viscosity of piroxicam loaded SLNs for transdermal drug administration. The drug release behaviors from piroxicam loaded SLNs based hydrogel exhibited long duration and constant rate of drug release over 24 hr. Skin penetration of piroxicam loaded SLNs based hydrogel was assessed by confocal laser scanning microscopy (CLSM).