High anticancer efficacy of solid lipid nanoparticles containing Zataria multiflora essential oil against breast cancer and melanoma cell lines

Alireza Valizadeh,Ali Asghar Khaleghi,Mahmoud Osanloo,Ghazaal Roozitalab

doi:10.1186/s40360-021-00523-9

Abstract

BackgroundThe cancer burden is rising rapidly worldwide, and it annually causes about 8.8 million deaths worldwide. Due to chemical drugs’ side effects and the emergence of resistance, the development of new green drugs has received much attention. We aimed to investigate whether solid-lipid nanoparticles containing essential oil of Zataria multiflora (ZMSLN) enhanced the anticancer efficacy of the essential oil against breast cancer (MDA-MB-468) and melanoma (A-375) cells.ResultsZMSLN was prepared by the high-pressure homogenizer method; particle size 176 ± 8 nm, polydispersity index 0.22 ± 0.1, entrapment efficiency 67 ± 5%. The essential oil showed a dose-dependent antiproliferative effect on MDA-MB-468 and A-375 cells at all examined concentrations (75, 150, 300, 600, and 1200 μg/mL). Interestingly, after treating both cells with 75 μg/mL of ZMSLN, their viabilities were reduced to under 13%.ConclusionThe finding showed that ZMSLN had a distinct antiproliferative efficacy; it could thus be considered a green anticancer candidate for further in vivo and in vivo studies.

Highlights

IntroductionEmotional, and physical burdens in all societies and comprise approximately 17% of global deaths [1]
Cancers have many financial, emotional, and physical burdens in all societies and comprise approximately 17% of global deaths [1]
The morphology of ZMSLN was demonstrated by TEM; it showed a spherical shape with a toothed shell-like

Summary

Introduction

Emotional, and physical burdens in all societies and comprise approximately 17% of global deaths [1]. The identification and development of novel anticancer using natural products with fewer serious adverse effects have received more attention [9, 10]. The Z. multiflora essential oil (ZMEO) possesses many biological activities, including anticancer and antioxidant effects [11, 12]. Solid-lipid nanoparticles (SLN) possess advantages over other colloidal systems, such as improved physical stability and sustaining the drug release [15, 16]. We describe here SLNs containing ZMEO (ZMSLN) model for natural indexing products with anticancer effects against breast cancer (MDA-MB-468) and melanoma (A-375) cells. We aimed to investigate whether solid-lipid nanoparticles containing essential oil of Zataria multiflora (ZMSLN) enhanced the anticancer efficacy of the essential oil against breast cancer (MDAMB-468) and melanoma (A-375) cells

Objectives

Methods

Results

Conclusion