Objectives: To assess the efficacy of mineral trioxide aggregate (MTA)pulpotomy in mature permanent teeth with irreversible pulpitis due to carious exposure. Placeand Duration: The study was conducted in the Department of Operative Dentistry, AltamashInstitute of Dental Medicine from 05-01-2016 to 05-07-2016. Methodology: A total of 70 teethof 70 patients with irreversible pulpitis were treated with mineral trioxide aggregate (MTA)pulpotomy. The patients were re-evaluated after 2 months. All patients were asked whether theyhad experienced spontaneous pain, pain on chewing or pain stimulated by hot/cold and thetreatment was counted as successful in case of no post-operative symptoms. Results: Efficacyof pulpotomy treatment with mineral trioxide aggregate in symptomatic mature permanentteeth with irreversible pulpitis and normal periapical periodontium was 88.57%. Conclusion:MTA pulputomy is a good alternative treatment for cases of irreversible pulpitis in permanentteeth with mature roots and normal apical periodontium. However, long term clinical trials andhistological assessment is desirable.