Biobased furfuryl alcohol (FOL) was a common building block for the production of resins, lubricants, plasticizers, organic compounds and value-added intermediates. For the effectual efficient production of FOL, a combination of chemocatalytic and biocatalytic approach was built for the transformation of D-xylose to FOL in a deep eutectic solvent (DES) lactic acid:betaine-H2O (LA:Bet-H2O) system. The furfural (FAL) yield of LA:Bet-H2O (10:90, vol/vol) for 30 min at 180 °C reached 81.6 % using D-xylose (150 g/L) as substrate in a high-pressure reactor. Using glucose as co-substrate, E. coli HMFOMUT whole cell could transform the formed FAL (125 mM) into FOL (96.8 % yield) in a conical flask at 37 °C and pH 7.0 in this LA:Bet-H2O (5:95, vol/vol) medium. In 50 mL LA:Bet-H2O, HMFOMUT cells effectually convert D-xylose-derived FAL to FOL, and 0.75 g of D-xylose formed FOL (0.384 g, 78.2 % yield) and furoic acid (FA) (0.019 g, 3.4 % yield). In the present study, a one-pot tandem method was used to effectually catalyze the synthesis of FOL from D-xylose using an effectual combination of chemocatalysis with LA:Bet and biocatalysis with E. coli HMFOMUT in a reactor. It affords a new idea for the effectual use of biomass resources to synthesize high value-added compounds, and also renders a mild, green, environmentally friendly and sustainable strategy for the manufacturing of bio-based platform derivatives.
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