A two-temperature-stage process was developed for the production of lipopeptides under SSF conditions. The effects of various temperatures, ranging from 25 to 40°C, on the bacterial growth during the growth stage and on the production of lipopeptides during the productive stage were investigated. The optimum temperatures were found to be 30°C for the growth of the strain and 37°C for the biosynthesis of lipopeptides. The two-stage fermentation temperatures should be 30°C in the initial 24h and then 37°C for the enhanced production of lipopeptides. The bioprocess results obtained in a 50L fermenter verified the efficacy of this technique, which increased the yield of lipopeptides by 8.40% in flasks and by 13.11% in the fermenter, with a 4h decrease of fermentation time in the fermenter. The 1000-fold scale-up of fermentation in a fermenter was successfully achieved.