Effects Of Tree Species Research Articles

Soil bacterial community characteristics and influencing factors in different types of farmland shelterbelts in the Alaer reclamation area

To investigate the effects of various types of farmland shelterbelts on soil quality and soil bacterial community diversity, this study focused on soil samples from four different shelterbelt types in the Alaer reclamation area, including Populus euphratica Oliv.- Populus tomentosa Carrière (PP), Elaeagnus angustifolia L.- Populus euphratica Oliv. (EP), Populus alba var. pyramidalis Bunge (P), and Salix babylonica L. (S). We analyzed their physical, chemical, biological properties as well as the differences in bacterial community structure, and explored the influencing factors on soil microbial community characteristics through microbial correlation network analysis. The results showed that: (1) There were significant differences in soil properties among the four types of farmland shelterbelts (p < 0.05), with P soils exhibiting the highest levels of organic matter, total nitrogen, and total phosphorus contents. (2) The Alpha diversity indices of soil bacteria showed significant differences among the four types of farmland shelterbelts (p < 0.05), with the P soils displayed the highest Chao1 and Shannon indices. (3) There were differences in the composition and abundance of dominant soil bacterial communities among different farmland shelterbelts, notably, the abundances of Verrucomicrobia, Acidobacteria, and Planctomycetes were significantly higher in P soils compared to the other three types. (4) The complexity of the correlation network between microbial species and environmental factors was highest in EP soils, soil microbial biomass nitrogen and available phosphorus were the main influencing factors. These findings indicated that different types of farmland shelterbelts had significant impacts on soil properties and soil bacterial communities. Soil bacterial communities were regulated by soil properties, their changes reflected a combined effect of soil characteristics and tree species.

Effects of urban green space habitats and tree species on ectomycorrhizal fungal diversity

Ectomycorrhizal fungi (EMF) are key symbiotic microbial components for the growth and health of trees in urban greenspace habitats (UGSHs). However, the current understanding of EMF diversity in UGSHs remains poor. Therefore, in this study, using morphological classification and molecular identification, we aimed to investigate EMF diversity in three EMF host plants: Cedrus deodara in the roadside green belt, and C. deodara, Pinus massoniana, and Salix babylonica in the park roadside green belt, in Guiyang, China. A total of 62 EMF Operational Taxonomic Units (OTUs) were identified, including 13 EMF OTUs in the C. deodara roadside green belt, and 23, 31, and 9 EMF OTUs in the park green belts. C. deodara, P. massoniana, and S. babylonica were respectively identified in park green belts. Ascomycota and Basidiomycota were the dominant phylum in the EMF communities in roadside and park green habitat, respectively. The Shannon and Simpson indexes of the C. deodara EMF community in the park green belt were higher than those in the roadside green belt. EMF diversity of the tree species in the park green belt was P. massoniana > C. deodara > S. babylonica. Differences in EMF community diversity was observed among the different greening tree species in the UGSHs. UGSHs with different disturbance gradients had a significant impact on the EMF diversity of the same greening tree species. These results can be used as a scientific reference for optimizing the design and scientific management of UGSHs.

Impact of ENVI-met-Based Road Greening Design on Thermal Comfort and PM2.5 Concentration in Hot–Humid Areas

Road greening markedly impacts road thermal comfort and air quality. However, previous studies have primarily focused on thermal comfort or PM2.5 individually, with relatively few addressing both aspects comprehensively, particularly in humid regions. This study combined field measurements and simulations. It employed physiological equivalent temperature (PET) and quantified the horizontal distribution of particulate matter 2.5 (PM2.5). The research examines the effects of planting spacing, tree species, and tree–shrub combinations on pedestrian walkways in humid climates during both summer and winter. Using measured tree data and road PM2.5, a plant model was established and pollution emission parameters were set to validate the effectiveness of the ENVI-met through fitting simulations under various scenarios. The results indicated that (1) plant spacing for trees influenced both the road thermal environment and PM2.5 levels. Smaller spacing improved thermal conditions but increased PM2.5. (2) trees with large canopies and high leaf area indices (LAIs) notably enhanced thermal comfort, while those with smaller canopies and dense understories facilitated PM2.5 dispersion. The 3 m spacing resulted in a maximum absolute PM2.5 concentration difference (C) of 5.05 μg/m3 in summer and a maximum mean absolute PM2.5 concentration difference (M) in the downwind region of 2.13 μg/m3 in winter. (3) Combining trees with shrubs moderately improved pedestrian thermal comfort. However, taller shrubs elevated PM2.5 concentrations on walkways; heights ranging from 1.5 m to 2 m in summer showed higher C values of 5.38 μg/m3 and 5.37 μg/m3. This study provides references and new perspectives for the optimization of roadway greening design in humid areas in China.

Tree species-mediated soil properties shape soil fauna community structure more strongly in the soil layer: Evidence from a common garden experiment

Soil fauna plays a crucial role in the soil biogeochemical cycles of forest ecosystems, with tree species composition regulating their diversity and functionality through alterations in habitat conditions and nutrient availability. However, identifying the impacts of tree species on soil faunal communities remains challenging due to the difficulty in distinguishing the effects of tree species-induced habitat changes from those of historical environmental conditions. Here, we conducted a field study in a common garden established in 2015, with consistent climate, soil, and land-use conditions to clarify the response of the soil faunal community to changes in tree species. After 5 years of plantation growth, we evaluated the differences in tree species identities and soil faunal community structures between the litter and soil layers in four closed-canopy broadleaf forests (Cinnamomum septentrionale, Cinnamomum camphora, Cinnamomum longepaniculatum and Toona sinensis). We found that the soil faunal communities differed significantly among the four broad-leaved tree species, with 18.7 % being dominated by Collembola and 67.2 % by Acari, with a relatively high proportion of microbivores (65.5 %). Compared with that of the other stands, the C. longepaniculatum stand had the most notable differences in taxa composition, while the T. sinensis stand exhibited the greatest soil biological quality, with a QBS value 1.25 times greater than that of other stands. The greatest total soil faunal abundance was observed in C. camphora, and this high abundance was due to a greater proportion of microbivores (75.4 %), whereas the detritivores, herbivores, and predators exhibited greater abundance in T. sinensis than in the other stands due to the high-quality litter input (low C/P and C/N ratios) and low tree biomass. The distribution of soil fauna across the habitat layers was significantly influenced by tree species changes, with the most pronounced differences occurring in the soil layer rather than in the litter layer. Finally, soil properties, rather than litter and plant conditions, were the primary factors explaining interspecific variations in total abundance and total diversity of the soil fauna and the QBS index, accounting for 61.6 %, 71.7 %, and 34.4 % of the variation, respectively. The results from the common garden experiment suggest that the change in the soil faunal community due to tree species identity was greater in the soil layer than in the litter layer, with the crucial determinant being tree-species-mediated soil properties. These insights enhance our understanding of the effect of tree species on soil faunal communities, which is essential for biodiversity conservation in artificial forests and for guiding tree species selection.

The interactive effect of tree mycorrhizal type, mycorrhizal type mixture and tree diversity shapes rooting zone soil fungal communities in temperate forest ecosystems

Abstract The underlying processes of plant‐microbe associations particularly their interactions with their mycorrhizal fungal partners have been extensively studied. However, considerably less is known about the consequences of tree‐tree interactions on rooting zone soil microbiota when tree species of different mycorrhizal type (myco‐type) grow together as mono and mixed myco‐type mixtures along a tree diversity gradient. Using the MyDiv tree diversity experiment, where arbuscular mycorrhizal (AM) and ectomycorrhizal (EcM) trees and their mixtures were planted in monocultures, two‐species and four‐species mixture plots, we investigated the interplay of target tree myco‐type, myco‐type mixture, tree diversity and rooting zone compartment (target tree dominated and its interaction zones with neighbour trees) on the rooting zone soil mycobiota employing meta‐barcoding of the ITS2 rDNA fragment of the fungal internal transcribed spacer (ITS). Our results revealed significant individual and interaction effects of tree myco‐type, myco‐type mixture and tree diversity but not rooting zone compartment on the fungal taxonomic and functional alpha and beta diversity. This implies intermingling of roots of target and neighbouring tree species there by reducing the target tree species effect in its rooting zone. As tree diversity increases, we found convergence of the fungal community in general, where the fungal community dissimilarity varies depending on the co‐existing tree species myco‐type and tree species diversity. Furthermore, the fungal community composition in the two and four species mixtures were consistently influenced by soil pH, whereas in the mixed multi‐species stands basal respiration, N, PO4−, NO3− were found to be equally important unlike in AM and EcM multi‐species stands. Comparative analysis of the fungal taxa specialisation between mono and mixed myco‐type multi‐species stands revealed that the mixed myco‐type plots shared 23.5% (AM) and 19.7% (EcM) of the generalist fungal communities However, the percentage of specialised fungal community in mixed myco‐type plots (13.2%) was significantly higher as compared to EcM (9.5%), and significantly lower (9%) as compared to AM (11.7%) plots, resulting in myco‐type and myco‐type mixture specific fungal communities and functional guild patterns Our results provide novel insights on the significance of tree species and its co‐existing trees preferred mycorrhizal association in shaping the target tree rooting zone soil mycobiome along a tree diversity gradient. Furthermore, it highlights the significance of generalist and specialist fungal communities in mono and mixed myco‐type stands in modulating tree‐tree interaction, tree species co‐existence and regulating soil properties and ecosystem functions. Read the free Plain Language Summary for this article on the Journal blog.

The influence of shade tree species and coffee varieties on selected soil physicochemical properties in coffee-based farming system of southwestern Ethiopia

Different shade tree species are used in various coffee production systems across the world. Outside the benefits of biodiversity protection, temperature protection, and carbon sequestration shade trees can influence soil nutrient states through litter inputs and nitrogen fixation. However, little information is available in coffee plantations whether shade tree species and type of coffee variety planted under shade tree have an influence on soil physical properties and nutrient status in coffee-based farming systems of southwestern Ethiopia. Hence, the study was carried out to investigate the effect of shade tree species and coffee varieties in coffee plantation on the physicochemical properties of the soil. The study was conducted in Chora Botor district (Chalalaki coffee plantation), located in Jimma Zone, Oromia regional states of Ethiopia. The study was superimposed on coffee farm that has been established using four released coffee varieties (7440,744, F59 and 75227) under three recommended coffee shade trees (Albizia gummifera, Millettia feruginea and Acacia abyssinica). Soil physical properties and nutrient status were investigated in response to shade tree species, coffee varieties and their combination. The results indicated that the physical and chemical properties of the soil vary across the shade trees and coffee variety grown. The effect of shade tree species on soil depends on the type of coffee varieties grown under the shade tree species, Albizia gummifera and Acacia abyssinica trees enhanced more soil nutrient content and water-holding capacity of the soil than Millttia ferrugnia. Total nitrogen, organic matter, available phosphorous, exchangeable potassium, Cations Exchange Capacity, and bulk density were higher underneath Albizia gummifera than other coffee shade tree species. On the other hand, moisture content, available P, exchangeable K and CEC were higher beneath Acacia abyssinica than other coffee shade tree species. Soil pH was negatively correlated with Millettia ferruginia. Use of Acacia abysinica and Albizia gummifera shade tree species with compatible coffee varieties could be a viable option to augment soil fertility management practices in the coffee production systems of the southwest Ethiopia.

Preliminary Study on the Chemical Properties of Non-Oak Wood Species: Suitability for Wooden Barrel Production

Oak wood (Quercus spp.) is the traditional and widely utilized raw material for wooden barrels deployed for the maturation of alcoholic beverages. The chemical compounds, extractives, lignin, hemicellulose, and others, in the wood are claimed to significantly influence the sensory attributes of the beverages during the aging process. The study aimed to assess the chemical properties of non-oak wood species. These species include Sandoricum koetjape (Burm. f.) Merr., Swietenia macrophylla King, Acacia mangium Willd., and Eucalyptus camaldulensis Dehnh., which are all locally available. The investigation was prompted by the limited availability and exorbitant fees associated with the importation of oak barrels into the country. The chemical properties of these non-oak wood species were compared to those of Quercus alba. The effects of tree species (TS) and tree portions (TP) on the chemical properties were evaluated using different TAPPI and ASTM methods. Results showed that S. macrophylla had the highest solubilities with 29.12, 14.64, and 9.09% in 1% sodium hydroxide solution, hot water, and ethanol-cyclohexane, respectively – higher than the solubilities of Q. alba in the three solvents. Meanwhile, E. camaldulensis had the highest lignin content (23.82%), whereas S. koetjape had the lowest lignin and S. macrophylla had the highest hemicellulose content. Q. alba had higher lignin content than all the species studied. The species’ alpha-cellulose contents were lower than that of Q. alba, whereas their holocellulose values were higher. Overall, the effect of TP (top, middle, and bottom) on the chemical composition of each of the wood samples was determined to have little to no effect on the chemical properties, hence indicating a relatively homogeneous distribution of chemical components from top to bottom. On the other hand, TS highly significant effect on the wood species on all the chemical properties analyzed. Overall, locally available non-oak wood species could be suitable as a raw material for wooden barrel fabrication. However, there is a need to conduct further studies on their anatomical, physico-mechanical, and other chemical properties.

Effects of tree and shrub species on soil quality, sediment detachment capacity caused by rills and surface slope stability in forest lands of Northern Iran

A root system is an important factor to increase soil resistance to detachment of soil particles. However, due to the large number of species, there is a need for studying the impacts of native plant species on soil quality and soil erodibility. This investigation did flume experiments at various soil slopes (9.2%, 18.1%, 25.1%, and 32.5%) and different water flow rates (0.56, 0.67, 0.74, 0.81, and 0.94 L/(m·s)), to evaluate sediment detachment capacity caused by rills (Dc) and rill erodibility (Kr) as well as the soil quality of hillslopes with three common species including Carpinus betulus (as a natural tree species), Alnus glutinosa (as a planted tree species) and Mespilus germanica (as a shrub species) in forestland of northern Iran. The variability of Dc has been associated with soil properties and root characteristics of Carpinus betulus. Dc was significantly lower (average, −45%) for soils under Carpinus betulus compared to soils with the two other plant species (p < 0.01). This was due to the higher values of soil properties including medium weight diameter of soil aggregates (MWD), organic carbon (OC), total nitrogen (TN), total phosphorous (TP), potassium (K), calcium (Ca), magnesium (Mg) as well as to the more extended root system, as confirmed by the negative correlations between Dc and the studied variables. Kr also was different among the studied soils and plant species. The root system of Carpinus betulus also played a useful role for increasing soil resistance to rill erosion yielding a safety factor (1.61) in the studied forest ecosystem. Overall, the current study supports a broader use of native species (such as Carpinus betulus) in areas exposed to surface erosion and instability, as an effective eco-engineering conservation technique and an alternative technology instead of utilizing artificial and expensive management practices.

Impact of Root Cutting on Acer platanoides and Tilia cordata Tree Stability in Urban Parks: A Case Study in Quebec City, Canada

Trees growing in urban environments are often impacted by maintenance or construction work involving the cutting of roots. Tree protection zones have been proposed to avoid critical damage to the tree. However, despite incorporating quantitative information, they heavily rely on expert judgement that remains to be validated. In a study conducted across six parks in Quebec City, Canada, two commonly found tree species, Acer platanoides L. and Tilia cordata Mill., presumed to be different in terms of vulnerability to root damage, were subjected to a range of trenching treatments. The trees were between 23 and 40 cm diameter at breast height (DBH). A safety factor was calculated relating the turning moment the tree can withstand to the turning moment imposed by high winds likely to occur. The safety factor against uprooting was assessed for each tree before and after root trenching using a non-destructive pulling approach. The effects of tree species, distance to the trench, and their combined interaction were tested on tree stability. The relationship between tree stability and soil texture, tree characteristics, and the number of damaged roots were also tested. Safety factors were initially variable, ranging from 0.5 to 4.5. T. cordata safety factors were lower than those of A. platanoides and influenced by soil texture. Trenching treatments had no effect on the safety factor, even when two perpendicular trenches were dug at 1 m from the stem. No index of the amount of root damaged was significantly related to the safety factor. Root trenching treatments that encroached closer to the tree trunk than the recommended tree protection zones did not affect the stability of both species. Nevertheless, it is essential to recognize that other ecophysiological processes might still be influenced, and long-term monitoring is crucial. Both should be taken into account when determining these zones.

Evaluating the effects of different tree species on enhancing outdoor thermal comfort in a post-industrial landscape

A frequently emphasized strategy to reduce the burden of heat in cities across the world is the implementation of street trees. Here, we examine the effects of deciduous and coniferous tree deployment on meteorological variables and pedestrian thermal comfort through analysis of the new dynamic thermal comfort (dPET) index, using the latest version of the computational fluid dynamics model ENVI-met. We performed on site observational measurements of air temperature (Ta), relative humidity (Rh), wind speed (Ws), and mean radiant temperature (MRT) at five different locations on the hottest day of summer 2023, in a post-industrial urban landscape located in Tehran, Iran. Observations were used to evaluate ENVI-met simulation performance and served as a baseline against which sensitivity experiments—based on a minimum (35%) and maximum (75%) intervention scenario for deciduous and coniferous trees—were compared against. Our analysis indicates that 35% and 75% deployment reduced Ta by 1.2 °C and 4.2 °C, respectively, for deciduous tree species, compared to a 0.9 °C and 3.1 °C reduction for coniferous species, during the hottest day of summer 2023. The maximum deployment scenario decreased MRT by approximately 60 °C and 43 °C for deciduous and coniferous tree deployment, respectively. The maximum tree deployment scenario decreased dPET by nearly 16 °C and 14 °C for deciduous and coniferous trees, respectively, during the time of day that diurnal heating is maximized. Our findings highlight micrometeorological and personalized thermal comfort effects associated with variable tree species type and extent through examination of a pedestrian’s ambulatory experience across diverse urban microclimates in a region of the world that is particularly understudied.

Effect of Different Multipurpose Tree Species on Physico-chemical Properties of Loamy Sand Soil Under Rainfed Conditions

A field experiment was conducted at Agroforestry Research Station, Sardarkrushinagar Dantiwada Agricultural University, Sardarkrushinagar, during 2019-20. The soils of the experimental field was low in organic carbon (0.24 per cent), available nitrogen (143.50 kg N/ha) and DTPA extractable iron (3.18 mg/kg), medium in available phosphorus (Olsen’s P 48.90 kg P2O5/ha), available potash (180.00 kg K2O/ha), manganese (7.8 mg/kg) and zinc (0.79 mg/kg) and high in copper content (0.61 mg/kg). An experiment consisting of six years old Agroforestry tree plantation which consisted of five multipurpose tree species (MPTs) viz., Tectona grandis, Eucalyptus camaldulensis, Gmelina arborea, Casuriana equstifolia and Melia azadiracht were designed in CRD with four replications. The experiment consisting of 96 trees per species with a plot size of 24 m × 16 m under 4 m × 4 m tree spacing. Among the above six tree species significantly the lowest BD (1.56 Mg/m3), higher WHC (27.87 per cent) and soil porosity (38.03 per cent) was recorded under Casuarinas equisetifolia tree species. Similarly the maximum mean organic carbon (0.445 per cent), available N (165.17 kg/ha), available K2O (196.26 kg/ha) and DTPA extractable iron (4.22 mg/kg) and copper (1.05 mg/kg) content were noted under Casuarina equisetifolia tree species. Whereas, the mean maximum available P2O5 (57.92 kg/ha) was noted under Tectona grandis. The mean maximum DTPA extractable manganese (8.34 mg/kg) and zinc (0.925 mg/kg) was noted under Eucalyptus camaldulensis and Melia azadiracht tree species, respectively.

Response of Soil Microbial Community Structure and Diversity to Mixed Proportions and Mixed Tree Species in Bamboo–Broad-Leaved Mixed Forests

Bamboo and broad-leaved mixed forests have been widely recognized for their advantages in maintaining ecological balance, improving soil fertility, and enhancing biodiversity. To understand the effects of mixed broad-leaved tree species and mixing ratios on soil microbial communities in bamboo and broad-leaved mixed forests, we quantified the structure and diversity responses of soil microbial communities to tree species and mixing ratios using high-throughput sequencing of the 16 S rRNA gene. Three bamboo and broad-leaved tree mixed forests were studied, including bamboo–Castanopsis chinensis Hance mixed forest (CCB), bamboo–Alniphyllum fortune (Hemsl.) Makino mixed forest (AFB), and bamboo–Choerospondias axillaris (Roxb.) B. L. Burtt &amp; A. W. Hill mixed forest (CAB). We assessed the impact of tree species and mixing ratios on soil microbial communities by measuring soil properties and the diversity and composition of soil microbes. The results indicate that soil properties and the diversity and composition of microbial communities are highly dependent on broad-leaved tree species in mixed forests. The mixing ratios had a more pronounced effect on microbial diversity than on richness. In CAB, diversity peaked at mixing ratios of 10%–20% and 20%–40%. The presence of broad-leaved trees significantly altered the relationships among soil bacteria, with CAB showing the highest stability, likely due to the increased diversity and quantity of litter from Choerospondias axillaris. Our results show that the choice of broad-leaved tree species and their mixing ratios significantly influence soil microbial diversity and composition in bamboo–broad-leaf mixed forests. An optimal mixing ratio in CAB can maximize bacterial diversity and stability, providing insights for forest management and promoting ecosystem health and sustainability.

Mixing trembling aspen and white spruce increases the understory vegetation cover and improves soil properties but effects vary with broadleaf density

Despite the importance of species mixtures to ecosystem-based forest management, density effect of broadleaf trees in the mixture on understory vegetation and soil properties are poorly understood. In this study, we examine the effects of trembling aspen [aspen] (Populus tremuloides Michx.) -white spruce [ spruce] (Picea glauca (Moench) Vos) mixtures that vary in the proportion of aspen on understory vegetation cover, soil nutrient supply rates, and soil properties. Data were collected from an established study located in Alberta, Canada at age 20 and included the following treatments: 1. Natural regeneration of pure aspen; 2. Retaining only 1600 planted spruce ha−1 by controlling aspen and understory vegetation; 3. Retaining only 1600 planted spruce ha−1 by controlling understory vegetation; 4–7. Retaining only 400 planted spruce ha−1 along with unthinned aspen, 2000, 1200, and 800 aspen ha−1. In mixed stands, competition control around the spruce was done for its establishment, and subsequent thinning was done to control the density of aspen. The findings of our analysis showed that mixed stands had higher total vascular plant cover, potential soil N supply rates, soil pH, and lowered C: N ratio compared to monocultures of spruce or aspen. The treatment (mixed stands) with unthinned aspen along with spruce increased the above-mentioned variables most compared to other mixtures. Moreover, total forb cover was higher in the mixture with the highest density of aspen, but grass cover was higher in the lowest density of aspen. Our findings hint that the effect of mixing tree species on understory vegetation cover may be influenced by the density of broadleaf tree in the mixture. Further studies need to be conducted to reach a concrete conclusion. Regardless, this result might be useful for forest managers to take a decision for ecosystem-based forest management.

Effects of tree species and planting forms on the thermal comfort of campsites in hot and humid areas of China.

Camping has become a popular outdoor activity in China. However, the long and scorching summers in China's hot and humid regions pose challenges for campsites in maintaining thermal comfort. Therefore, we explored the impact of tree species and planting methods on the thermal comfort of urban campsites in hot and humid areas using the ENVI-met model to simulate the conditions of the study area. The reliability of the model was validated by comparing the simulated values of air temperature (Ta) and relative humidity (RH) with field measurements. We conducted an in-depth analysis of common trees in hot and humid areas and analyzed the effects of five tree species and four tree planting forms on the microclimate of campsites in such areas, using the physiological equivalent temperature (PET) as the evaluation index of thermal comfort. The results indicated that: (1) trees with larger crown widths were most effective in improving outdoor thermal comfort. The ability of trees to regulate microclimate was more influenced by crown width than by leaf area index (LAI), and (2) trees planted in patches provided the highest level of thermal comfort, whereas single trees provided the lowest. However, relying solely on tree planting made it difficult to significantly reduce outdoor heat stress. Therefore, other methods such as increasing ventilation or mist spray should be adopted to modify camping area. This study provides a reference for the planting design of outdoor campsites in hot and humid regions of China.

Will large-scale forestation lead to a soil water deficit crisis in China's drylands?

Trading water for carbon has cautioned large-scale afforestation in global drylands. However, model simulations suggested that the consumption of soil water could be partially offset by increasing precipitation due to vegetation feedback. A systematic meta-analysis of long-term and large-scale field observations is urgently required to address the abovementioned limitations, and the implementation of large-scale afforestation since 1978 in northern China provides an ideal example. This study collected data comprising 1226 observations from 98 sites in northern China to assess the variation in soil water content (SWC) with stand age after afforestation and discuss the effects of tree species, precipitation and conversions of land use types on SWC. We found that the SWC has been decreased by coniferous forest and broadleaf forest at rates of 0.6 and 3.2 mm decade–1, respectively, since 1978. There is a significant declining trend of SWC with the stand age of plantations, and the optimum growth stage for plantation forest is 0–20 a in northern China. However, we found increases in SWC for the conversion from grassland to forest and in the low-precipitation region, both are corresponding to the increased SWC in coniferous forest. Our study implies that afforestation might lead to a soil water deficit crisis in northern China in the long term at the regional scale but depends on prior land use types, tree taxa and the mean annual precipitation regime, which sheds light on decision-making regarding ecological restoration policies and water resource management in drylands.

Biological soil health indicators are sensitive to shade tree management in a young cacao (Theobroma cacao L.) production system

Cacao agroforestry systems can offer important benefits, such as greenhouse gas mitigation, microclimate regulation and improved soil health. The selection of tree species for cacao agroforestry systems is a critical step impacting cacao yields, as well as the environmental and economic sustainability of the production system. However, the effects of different tree species on soil processes and functions have been poorly studied. We assessed a series of soil health indicators in a five-year-old agroforestry trial located in the Ecuadorian Amazon. The following treatments: “control” (cacao monoculture), “timber” (cacao with Cedrelinga cateniformis Ducke; a leguminous tree), “fruit” (cacao with Bactris gasipaes), “N-fix” (cacao with Erythrina velutina Wild) and “mixed” (cacao with C. cateniformis + E. velutina) were replicated 3 times in randomized blocks. The experiment was managed organically with low levels of external inputs. We collected soil, litter and leaf samples of cacao trees at two different distances (∼2 m and ∼ 6 m) from the shade tree. Biological (potential soil respiration, and macrofauna abundance, richness and Shannon diversity), chemical (pH, CEC, total C and N, macro and micronutrients and Cd), and physical (bulk density and water holding capacity) soil health indicators were measured. Additionally, we analysed nutrients and Cd in cacao leaves and litter. Results showed positive effects of the “timber” and “mixed” treatments on biological soil health indicators, primarily earthworm abundance and potential respiration, as compared to cacao monocultures. Treatment effects on potential respiration showed different trends in litter versus soil, indicating that effects of shade trees on litter respiration do not transfer directly to soil respiration. Physical and chemical soil indicators, including available Cd which is irrelevant in the context of food safety regulations, did not show any differences among the treatments. Five years after establishment, no significant differences in yields were found among the control and any of the shade tree treatments.

Afforestation, Natural Secondary Forest or Dehesas? Looking for the Best Post-Abandonment Forest Management for Soil Organic Carbon Accumulation in Mediterranean Mountains

Forest expansion in Mediterranean mountain areas is a widespread phenomenon resulting from the abandonment of agricultural and pastoral activities during the last century. Therefore, knowledge of the long-term storage capacity of soil organic carbon (SOC) in Mediterranean forests is of great interest in the context of global change. However, the effects of these land uses and covers (natural secondary forest, afforestation with conifers and silvo-pastoral ecosystems (dehesas)) on SOC dynamics are still uncertain. The main objectives of this study were to evaluate physico-chemical soil properties, SOC and nitrogen stocks, and SOC fractions in Mediterranean forests and to assess the effects of tree species, the soil environment (acidic and alkaline), and land management. We selected five land uses and land covers: managed and non-managed afforestation and dehesa (except for alkaline dehesa) and a stage of succession when tree species begin to become established after abandonment. This study concludes that although total SOC stocks are higher in afforested systems with conifers, SOC is stored in less stable carbon pools than in broadleaf forests. In addition, this study confirms that there are marked differences in the results between acidic and alkaline environments. Finally, the management system is also a significant factor, particularly for afforested sites.

Effects of tree species mixing and soil depth on the soil bacterial and fungal communities in Chinese fir (Cunninghamia lanceolata) plantations

Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] is a critical timber tree species for its fast growth and high yield in southern China and is widely planted in subtropical regions of the country. However, the management of Chinese fir in the form of a monoculture plantation is not conducive to the maintenance of soil fertility and improvements in forest productivity. The establishment of mixed conifer–broad–leaved forests through near-natural transformation is developing into a promising method of forest cultivation for replacing large pure coniferous plantations. Bacteria and fungi are an important part of the soil microbiota and are involved in the soil nutrient cycle. Therefore, this study investigated the response of soil bacterial and fungal communities to the introduction of broad–leaved trees in three types of Chinese fir plantations [Cunninghamia lanceolata monoculture plantation (CMP), C. lanceolata–Castanopsis hystrix Hook. f. & Thomson ex A. DC.–Michelia hedyosperma Law. mixed plantation (CCM), and C. lanceolata–Castanopsis fissa (Champ. ex Benth.) Rehder & E.H.Wilson–Erythrophleum fordii Oliv. mixed plantation (CCE)] located in Guangxi Province, China. And the effect of soil layers (0–20, 20–40, and 40–60 cm soil depths) on the soil microbial communities was further analyzed. The results suggested that tree species mixing significantly affected bacterial and fungal communities, but soil depth only significantly affected bacterial communities (P < 0.01). The relative abundance of Basidiomycetes in CCE (53.91–78.76 %) was significantly higher than that in pure forest (P < 0.05). Bacteria exhibited more complex and multilinked networks in the mixed stands, particularly for the CCE plantation. Mixed planting patterns significantly enhanced bacterial nitrogen fixation and the presence of symbiotic fungi. Redundancy analysis (RDA) and correlation analyses indicated that the microbial community structure was strongly affected by soil environmental factors, with bacteria affected mainly by available phosphorous (AP) and fungi affected mainly by soil enzyme activity and total potassium (TK). In summary, the introduction of broad–leaved species (especially C. fissa and E. fordii) had a significant impact on the soil microbial community, increasing the relative abundance of bacterial nitrogen fixation and symbiotic fungi and generating a complex microbial network. We suggest that changes in AP, TK, and fungal communities should be considered in the near-natural transformation of Chinese fir or soil fertility maintenance and sustainable management to maintain soil fertility.

No effect of invasive tree species on aboveground biomass increments of oaks and pines in temperate forests

Prunus serotina and Robinia pseudoacacia are the most widespread invasive trees in Central Europe. In addition, according to climate models, decreased growth of many economically and ecologically important native trees will likely be observed in the future. We aimed to assess the impact of these two neophytes, which differ in the biomass range and nitrogen-fixing abilities observed in Central European conditions, on the relative aboveground biomass increments of native oaks Quercus robur and Q. petraea and Scots pine Pinus sylvestris. We aimed to increase our understanding of the relationship between facilitation and competition between woody alien species and overstory native trees. We established 72 circular plots (0.05 ​ha) in two different forest habitat types and stands varying in age in western Poland. We chose plots with different abundances of the studied neophytes to determine how effects scaled along the quantitative invasion gradient. Furthermore, we collected growth cores of the studied native species, and we calculated aboveground biomass increments at the tree and stand levels. Then, we used generalized linear mixed-effects models to assess the impact of invasive species abundances on relative aboveground biomass increments of native tree species. We did not find a biologically or statistically significant impact of invasive R. pseudoacacia or P. serotina on the relative aboveground biomass increments of native oaks and pines along the quantitative gradient of invader biomass or on the proportion of total stand biomass accounted for by invaders. The neophytes did not act as native tree growth stimulators but also did not compete with them for resources, which would escalate the negative impact of climate change on pines and oaks. The neophytes should not significantly modify the carbon sequestration capacity of the native species. Our work combines elements of the per capita effect of invasion with research on mixed forest management.

Effects of mixed broadleaved tree species with pure Pinus massoniana plantation on soil microbial necromass carbon and organic carbon fractions

Mixing native broadleaved tree species is a widely used method for renovating Pinus massoniana plantations. Soil microbial necromass carbon and organic carbon fractions are important parameters for evaluating the impacts of tree species mixing and soil organic carbon (SOC) stability. However, their responses to the mixing and renovation of P. massoniana plantation has not been understood yet. Here, we selected a pure P. massoniana plantation (PP) and a mixed P. massoniana and Castanopsis hystrix plantation, with ages of 16 (MP16) and 38 years (MP38), respectively, as the research objects. We quantified soil physical and chemical properties, microbial necromass carbon content, and organic carbon components at different soil layers to reveal whether and how the introduction of C. hystrix into P. massoniana plantation affected soil microbial necromass carbon and organic carbon components. The results showed that the mixed P. massoniana and C. hystrix plantation significantly reduced fungal necromass carbon content and the ratio of fungal/bacterial necromass carbon in the 0-20 cm and 20-40 cm soil layers. There were no significant differences in microbial necromass carbon contents, bacterial necromass carbon contents, and their contributions to SOC among the different plantations. The contribution of fungal necromass carbon to SOC was higher than that of bacterial necromass carbon in all plantation types. The contribution of soil mineral-associated organic carbon (MAOC) to SOC was higher than that of occluded particulate organic carbon (oPOC) and light-free particulate organic carbon (fPOC) for all plantation types. Mixing the precious broadleaved tree species (i.e., C. hystrix) with coniferous species (P. massoniana) significantly increased MAOC content and the contribution of MAOC, oPOC, and fPOC to SOC in the 0-20 cm and 20-40 cm soil layers. The MAOC of MP38 was significantly higher than that of PP in all soil layers and the MAOC of MP38 stands were significantly higher than MP16 stands in the 20-40 cm, 40-60 cm, and 60-100 cm soil layers, indicating that hybridization enhanced SOC stability and that the SOC of MP38 stands were more stable than MP16 stands. SOC and total nitrogen contents were the main environmental factors driving the changes in soil microbial necromass carbon, while soil total nitrogen and organically complexed Fe-Al oxides were the primary factors affecting organic carbon fraction. Therefore, SOC stability can be enhanced by introducing native broadleaved species, such as C. hystrix, during the management of the P. massoniana plantation.