Effects of tree species mixing and soil depth on the soil bacterial and fungal communities in Chinese fir (Cunninghamia lanceolata) plantations

Abstract

Chinese fir [Cunninghamia lanceolata (Lamb.) Hook.] is a critical timber tree species for its fast growth and high yield in southern China and is widely planted in subtropical regions of the country. However, the management of Chinese fir in the form of a monoculture plantation is not conducive to the maintenance of soil fertility and improvements in forest productivity. The establishment of mixed conifer–broad–leaved forests through near-natural transformation is developing into a promising method of forest cultivation for replacing large pure coniferous plantations. Bacteria and fungi are an important part of the soil microbiota and are involved in the soil nutrient cycle. Therefore, this study investigated the response of soil bacterial and fungal communities to the introduction of broad–leaved trees in three types of Chinese fir plantations [Cunninghamia lanceolata monoculture plantation (CMP), C. lanceolata–Castanopsis hystrix Hook. f. & Thomson ex A. DC.–Michelia hedyosperma Law. mixed plantation (CCM), and C. lanceolata–Castanopsis fissa (Champ. ex Benth.) Rehder & E.H.Wilson–Erythrophleum fordii Oliv. mixed plantation (CCE)] located in Guangxi Province, China. And the effect of soil layers (0–20, 20–40, and 40–60 cm soil depths) on the soil microbial communities was further analyzed. The results suggested that tree species mixing significantly affected bacterial and fungal communities, but soil depth only significantly affected bacterial communities (P < 0.01). The relative abundance of Basidiomycetes in CCE (53.91–78.76 %) was significantly higher than that in pure forest (P < 0.05). Bacteria exhibited more complex and multilinked networks in the mixed stands, particularly for the CCE plantation. Mixed planting patterns significantly enhanced bacterial nitrogen fixation and the presence of symbiotic fungi. Redundancy analysis (RDA) and correlation analyses indicated that the microbial community structure was strongly affected by soil environmental factors, with bacteria affected mainly by available phosphorous (AP) and fungi affected mainly by soil enzyme activity and total potassium (TK). In summary, the introduction of broad–leaved species (especially C. fissa and E. fordii) had a significant impact on the soil microbial community, increasing the relative abundance of bacterial nitrogen fixation and symbiotic fungi and generating a complex microbial network. We suggest that changes in AP, TK, and fungal communities should be considered in the near-natural transformation of Chinese fir or soil fertility maintenance and sustainable management to maintain soil fertility.