Effect Of Thermotherapy Research Articles

Enhanced effect of silver nanoparticles combined with thermotherapy on rat C6 glioma cells

Objective To investigate the effect of silver nanoparticles (AgNPs) on rat glioma C6 cells upon the combination of hyperthermia treatment (HTT).Methods The polyol process and transmission electron microscopy (TEM) were used to synthesize the AgNPs.Methylthiazol tetrazolium (MTT) assay was used to analyze the cytotoxicity of AgNPs.The enhance effect of AgNPs in combination with HTT was detected by using colony-forming assay and TEM.Results AgNPs were synthesized and the mean size was 15 nm.The particles showed dose-dependent cytotoxicity on C6 cells from the experimental data [50％inhibitory concentration (IC50):4 mg/L].Survival rate in heating (44℃C 15 min) combined with AgNPs group was 10.5％,and that in the control group was up to 39.7％.Conclusion AgNPs could increase the effect of thermotherapy on rat C6 glioma cells by inducing cytotoxicity and enhancing the thermosensitivity. Key words: Gliomas ; Silver nanoparticles; Hyperthermia

Effectiveness of Thermotherapy Using a Heat and Steam Generating Sheet for Cartilage in Knee Osteoarthritis

[Purpose] Superficial heat treatment is one of the most widely used physical therapies for osteoarthritis (OA). We clinically evaluated the effects of local heat treatment and exercise therapy for knee OA, and evaluated the articular cartilage using magnetic resonance imaging (MRI) T2 mapping. [Subjects and Methods] Eighteen females aged 50–69 (59.5 ± 8.5 years, mean ± SD) years diagnosed with early-stage knee OA were randomly assigned using computer-generated random numbers to either a local heat treatment group (LH group, 9 subjects) or an exercise therapy group (EX group, 9 subjects). These groups were subjected to a 12-week intervention experiment. MRI T2 mapping was performed for cartilage imaging and quantitative evaluation. For clinical evaluation, the Japanese Knee Osteoarthritis Measure (JKOM) and the Timed Up and Go (TUG) test were performed. Both clinical and MRI evaluations were performed at the beginning and end of the intervention (0 week (Time 0) and 12 weeks). [Results] The total JKOM score had a significantly decreased in the LH group at 12 weeks. However, in the EX group the total JKOM scores at Time 0 and 12 weeks were not significantly different. The TUG time in the EX group was significant shorter at 12 weeks, whereas it showed no significant change in the LH group at 12 weeks, though the TUG times of 7 of the 9 patients decreased, exhibiting some improvement. The T2 value of the LH group was significantly shorter at 12 weeks. However, the T2 value in the EX group showed no significant change at 12 weeks. [Conclusion] After local heat treatment using heat- and steam moisture-generating sheets for 12 weeks, we observed improvements in clinical symptoms and walking abilities. Moreover, positive effects on cartilage metabolism were suggested.

Nanocomposites: A Low‐Toxic Multifunctional Nanoplatform Based on Cu9S5@mSiO2 Core‐Shell Nanocomposites: Combining Photothermal‐ and Chemotherapies with Infrared Thermal Imaging for Cancer Treatment (Adv. Funct. Mater. 35/2013)

J. Hu and co-workers successfully develop mesoporous silica-coated copper with a core-shell nanostructure (Cu9S5@mSiO2) as a versatile multifunctional platform. On page 4281, the Cu9S5@mSiO2 shows low cytotoxicity and excellent blood compatibility, and is effective for both photothermal therapy and infrared thermal imaging. Moreover, it can deliver Doxorubicin into cancer cells for chemotherapy, which enhances the effect of thermotherapy. These results will encourage development of such a nanoplatform, synergetically combining thermo- and chemotherapy for real potential in the clinic.

Effects of thermotherapy combined with indomethacin on proliferation and invasiveness of hepatocellular carcinoma cell line MHCC97L

Objective To investigate the effects of thermotherapy combined with indomethacin on proliferation and invasiveness of hepatocellular carcinoma cell line MHCC97L.Methods Indomethacin (0.2 mmol/L) was added 2 h before thermotherapy.Chemicals were omitted for the thermotherapy group.Chemicals and thermotherapy were omitted for the control group.Proliferation,growth curve and plate efficiency were observed.Flow cytometry was used to analyze the cell cycle.Transwell invasion assay and cell motility assay were done.The expression ov vascular endothelial growth factor (VEGF) and matrix metalloproteinase-2 (MMP-2) protein in MHCC97L cells was detected by using enzyme-linked immunosorbent assay.Results Compared to the control group,the proliferation of MHCC97L cells was significantly decreased after thermotherapy combined with indomethacin (P ＜ 0.01).The inhibition ratio reached the maximum at 48 h after thermotherapy up to 53.6％,and the doubling time increased 2.07 times.The plate efficiency of the same number of cells 48 and 96 h after thermotherapy combined with indomethacin was decreased (P ＜0.01).Flow cytometry revealed that the ratio of cells in G1 phase was increased and that in S + G2 phase decreased 48 and 96 h after thermotherapy combined with indomethacin,respectively (P ＜0.01,P ＜ 0.05).The average amount of invading cclls per field in cell invasion assay and motility assay of the same number of cells 48 and 96 h after thermotherapy combined with indomethacin was significantly less than solitary thermotherapy group,respectively (P ＜ 0.01).Compared to solitary thermotherapy group,the expression of MMP-2 and VEGF 48 and 96 h after thermotherapy combined with indomethacin was decreased,respectively (P ＜ 0.05).Conclusion Indomethacin suppressed thermotherapy-augmented proliferation of MHCC97L cells,which was in part mediated by inhibiting DNA synthesis and mitosis of the cells.Indomethacin further inhibited the invasiveness of the cells,which was associated with the reduced VEGF and MMP-2 protein expression. Key words: Hepatocellular carcinoma; Thermotherapy; Indomethacin; Proliferation; Invasiveness; Vascular endothelial growth factor; Matrix metalloproteinase

Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway

Mechanisms of synergistic effect of thermotherapy and chemotherapy on human lung adenocarcinoma cell growth are unclear. The purpose of this study was to investigate these effects and explore the function of ROS, Akt and caspase-3 in relation to these. A549 cells were subjected to different thermochemotherapy treatments: 43˚C heat+50 µg/l paclitaxel (thermochemotherapy group), 43˚C heat+50 µg/l paclitaxel+1 µmol/l wortmannin (wortmannin group), 43˚C heat+50 µg/l paclitaxel+30 µmol/l NAC (NAC group) and 50 µg/l paclitaxel (chemotherapy group). The cells without any treatment were regarded as controls. Cell proliferation rates were measured with MTT assay and intracellular ROS levels were detected with fluorescence labeling technologies. Phosphorylation of Akt and caspase-3 expression were determined by western blotting and the cell apoptosis rates were examined by flow cytometry. Tests in vivo were carried out at the same time. It was found that the cell proliferation rates of the thermochemotherapy group were significantly lower compared to those of the controls or the chemotherapy group (P<0.05). The intracellular ROS levels of the thermochemotherapy group were elevated significantly compared with those of other groups, and these changes could be reversed using the ROS inhibitor NAC but not a PI3K inhibitor (wortmannin). Phosphorylation of Akt was significantly decreased in the thermochemotherapy group (P<0.05), which could be blocked by wortmannin, but increased by NAC (P<0.05). The caspase-3 expression levels and cell apoptosis rates of the thermochemotherapy group were higher compared to those of the other groups (P<0.05). All results have been confirmed by in vivo tests. Thus, the combination of thermotherapy with chemotherapy showed a stronger inhibitory effect on A549 cell growth compared to chemotherapy alone, which may be able to cause additional cell apoptosis through inhibition of Akt phosphorylation and activation of caspases by increased intracellular ROS production.

Métodos de detecção de Leifsonia xyli subsp. xyli e efeito da termoterapia na brotação das gemas de diferentes variedades de cana-de-açúcar

Ratoon stunting disease [Leifsonia xyli subsp xyli (Lxx)] is one of the main diseases of sugarcane and it is of difficult detection in the field. Its spread to uninfected plants and into new areas occurs exclusively through contaminated planting material or mechanically. Thermotherapy at a 52oC/30 min regime is broadly employed in Brazil for producing healthy seed cane. Another recommended regime (50oC/2h) has not been commonly used in Brazil. The purposes of this research were to examine the efficacy of different methods of detection of Lxx in 4-9 months-old plants in cane varieties having different periods of maturity and the effect of thermotherapy (50oC/2h) on bud germination including a consideration on the location of individual buds in the stalks. Microscopy was the best method allowing the detection of Lxx in plants of all ages whereas identification of Lxx in infected plants through dot blot and PCR methods, performed according to published protocols, failed for all treatments. A modification of the dot blot test involving the use of concentrated cane sap was successful in improving the method, allowing the identification of Lxx in infected nine-month old plants. Thermotherapy at a regime of 50oC/2h did not completely eradicate Lxx from infected planting material. This regime was harmful for the germination of buds in var. RB935744 but not for vars RB855156 and RB867515. It increased the percentage of germination of basal buds of RB867515 and decreased that of apical and mid length buds of RB935744.

Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway

Involvement of ROS in the inhibitory effect of thermotherapy combined with chemotherapy on A549 human lung adenocarcinoma cell growth through the Akt pathway

Magnetic nanoparticle-based hyperthermia for head & neck cancer in mouse models.

In this study, magnetic iron oxide nanoparticle induced hyperthermia is applied for treatment of head and neck cancer using a mouse xenograft model of human head and neck cancer (Tu212 cell line). A hyperthermia system for heating iron oxide nanoparticles was developed by using alternating magnetic fields. Both theoretical simulation and experimental studies were performed to verify the thermotherapy effect. Experimental results showed that the temperature of the tumor center has dramatically elevated from around the room temperature to about 40oC within the first 5-10 minutes. Pathological studies demonstrate epithelial tumor cell destruction associated with the hyperthermia treatment.

DETECTION OF TOMATO MOSAIC VIRUS IN TOMATO SEED AND TREATMENT BY THERMOTHERAPY

DETECTION OF TOMATO MOSAIC VIRUS IN TOMATO SEED AND TREATMENT BY THERMOTHERAPY

MP-08.04 Effects of Thermotherapy and Medical Therapy for Chronic Prostatitis/Chronic Pelvic Pain Syndrome: A Prospective Study

Chronic prostatitis/chronic pelvic pain syndrome (CP/CPPS) is long-term pain and urinary symptoms that involve the prostate or other parts of a man's lower urinary tract or genitalia. The treatment goal of CP/CPPS is to control symptoms, because a cure is difficult to achieve. We analyzed the clinical outcomes of combination treatment of thermotherapy using URO-Dr™ and medical therapy.

Co-Inhibition of HSP70/HSP90 Synergistically Sensitizes Nasopharyngeal Carcinoma Cells to Thermotherapy

The upregulation of both HSP70 and HSP90 frequently compromises the effects of thermotherapy. The co-inhibition of HSP70/HSP90 may be preferable to enhance the effects of thermotherapy on nasopharyngeal carcinoma cells. The changes of HSP70 and HSP90 were detected after thermotherapy in human nasopharyngeal cancer cell HNE1. 17-Dimethylaminoethylamino-17-demethoxygeldanamycin (17-DMAG) and quercetin were used to inhibit the activity of HSP90 and HSP70. The enhanced effects were evaluated in vitro and in vivo. Both HSP70 and HSP90 were upregulated promptly in HNE1 after thermotherapy. Single inhibition of HSP70 resulted in overexpression and delayed descent of HSP90. The co-inhibition of HSP70/HSP90 with quercetin plus 17-DMAG significantly increased apoptosis in hyperthermia-treated HNE1 cells both in vitro and in vivo. The co-inhibition of HSP70/HSP90 synergistically sensitizes nasopharyngeal carcinoma cells to hyperthermia.

Qualidade sanitária e fisiológica de sementes de Amburana cearensis A.C. Smith submetidas à termoterapia e tratamento químico

The objective of this work was to evaluate the effectiveness of thermotherapy in the control of fungi associated with seeds of cumaru ( Amburana cearensis A.C. Smith) from two localities, after thermal and chemical treatment. The health assessment was conducted by the method of incubation on paper filter (Blotter Test) and physiological quality seeds was determined by germination tests and electrical conductivity. The thermal treatment was used by immersion in water heated to 60 degrees for 5, 10 and 20 minutes. The chemical treatment was done with Captan fungicide. The study used 100 seeds per treatment (10 seeds per petri dish), which were incubated for seven days, at a temperature of 22 ± 2°C and 12-hour photoperiod. The qualitative and quantitative assessment (expressed in percentage) of the fungi associated with the seeds was done with the help of a stereoscope. In the examined samples, only the Aspergillus niger fungus was found. There was a higher incidence of A. niger in the lot from the town of Catole Rocha, Paraiba State, with very low incidence of the fungus in the town Petrolina, Pernambuco State. The treatments with the best results were fungicide and thermotherapy for 20 minutes.

Preparation and biodistribution of 188Re-labeled folate conjugated human serum albumin magnetic cisplatin nanoparticles (188Re-folate-CDDP/HSA MNPs) in vivo

BackgroundThe purpose of this study was to develop intraperitoneal hyperthermic therapy based on magnetic fluid hyperthermia, nanoparticle-wrapped cisplatin chemotherapy, and magnetic particles of albumin. In addition, to combine the multiple-killing effects of hyperthermal targeting therapy, chemotherapy, and radiotherapy, the albumin-nanoparticle surfaces were linked with radionuclide 188Re-labeled folic acid ligand (188Re-folate-CDDP/HSA).MethodsHuman serum albumin was labeled with 188Re using the pre-tin method. Reaction time and optimal conditions of labeling were investigated. The particles were intravenously injected into mice, which were sacrificed at different time points. Radioactivity per gram of tissue of percent injected dose (% ID/g) was measured in vital organs. The biodistribution of 188Re-folate-CDDP/HAS magnetic nanoparticles was assessed.ResultsOptimal conditions for 188Re-labeled folate-conjugated albumin combined with cisplatin magnetic nanoparticles were: 0.1 mL of sodium gluconate solution (0.3 mol/L), 0.1 mL of concentrated hydrochloric acid with dissolved stannous chloride (10 mg/mL), 0.04 mL of acetic acid buffer solution (pH 5, 0.2 mol/L), 30 mg of folate-conjugated albumin combined with cisplatin magnetic nanoparticles, and 188ReO4 eluent (0.1 mL). The rate of 188Re-folate-CDDP-HSA magnetic nanoparticle formation exceeded 90%, and radiochemical purity exceeded 95%. The overall labeling rate was 83% in calf serum at 37°C. The major uptake tissues were the liver, kidney, intestine, and tumor after the 188Re-folate-CDDP/HSA magnetic nanoparticles were injected into nude mice. Uptake of 188Re-folate-CDDP/HSA magnetic nanoparticles increased gradually after injection, peaked at 8 hours with a value of 8.83 ± 1.71, and slowly decreased over 24 hours in vivo.ConclusionThese results indicate that 188Re-folate-CDDP/HSA magnetic nanoparticles can be used in radionuclide-targeted cancer therapy. Surface-modified albumin nanoparticles with folic acid ligand-labeled radionuclide (188Re) were successfully prepared, laying the foundation for a triple-killing effect of thermotherapy, chemotherapy, and radiation therapy.

Interstitial laser thermotherapy of a rat liver tumour: Effect of hepatic inflow occlusion

Interstitial laser thermotherapy was used to treat rat liver tumours. The aim was to investigate the influence of temperature and temporary hepatic inflow occlusion on tumour growth and blood perfusion. Liver tumours were treated at 44°C at the tumour border for 30 minutes, hepatic inflow occlusion only, or a combination of these methods. Interstitial laser Doppler flowmetry was used to measure hepatic perfusion at the tumour border during and after heat treatment, for a total time of 60 minutes. Tumour growth was evaluated 6 days after treatment. Tumours subjected to the combined treatment of hepatic inflow occlusion and interstitial laser thermotherapy displayed a blood perfusion reduction 30 minutes after treatment to 18 ± 5% of initial perfusion, which was significantly lower than achieved with thermotherapy alone (52 ± 10%, P = 0.02). The combined treatment and treatment with thermotherapy alone resulted in relative tumour growth of 0.3 ± 0.1 and 1.0 ± 0.2, respectively (P = 0.04). Inflow occlusion enhanced the effect of thermotherapy not by augmenting treatment temperatures but by increasing the thermal sensitivity of the tumour, reflected by an immediate effect on tumour blood perfusion.

Effects Of Thermotherapy And Cryotherapy On Muscle Hypertrophy And Muscle Contraction Force Following Resistance Exercise

INTRODUCTION: Physical therapists seek to improve skeletal muscle contraction force and muscular hypertrophy in the most time efficient manner. Different types of modalities that are applied post-therapy may have an effect on these improvements. PURPOSE: To examine the effects of thermotherapy (THERM) and cryotherapy (CRYO) on skeletal muscle contraction force and muscle hypertrophy of the biceps muscle following a high volume and medium intensity resistance exercise program. METHODS: Thirty one (31) subjects were randomly assigned to THERM, CRYO and control (CON) groups. Upper arm girth measurements (1/3, 1/2, and 2/3 of the upper arm length) and 1-RM tests were performed prior to any resistance training, and at 2, 4, and 6 weeks during training. RESULTS: Results showed no significant % change (p> 0.05) between the treatments on muscle hypertrophy (THERM: 0.804+3.3%; CRYO: -0.123+1.6%; CON: 0.286+1.5%) and skeletal muscle contraction force (THERM: 25.57+12.0%; CRYO: 19.15+14.0%; CON: 26.54+21.8%) following six weeks of resistance exercise program. A significant (p<0.0001) increase in skeletal muscle contraction force over time was observed in all three groups. CONCLUSIONS: These data indicate that neither modality resulted in a significant change in muscle contraction force or muscle hypertrophy following resistance exercise, however it does demonstrate a significant increase in skeletal muscle contraction force over time.

Effects of Thermotherapy with a Bath Additive Containing a Vasodilator on Chronic Shoulder-Neck Pain -Comparison with the Existing Remedies

The purpose of this study was to investigate the effects of thermotherapy using a bath additive containing a vasodilation agent on chronic shoulder-neck pain in comparison with conventional remedies. Male subjects reporting chronic shoulder-neck muscle pain and receiving frequent established remedies (group A: acupuncture; group B: massage; and group C: over-the-counter medicine) participated in this study. These treatments were replaced by treatment with carbon dioxide baths containing 3-octylphthalide (KYN) for 2 weeks. Changes in the subjects' symptoms were evaluated during the 2 weeks of KYN use and the following 2 weeks with a return to the initial treatments. Differences in the effects of treatment with KYN were found among these groups. In group B, significant decreases in VAS of overall symptoms in the shoulder-neck region, feelings of muscle tension, pain and difficulty in movement of the shoulder joint, and muscle hardness were found during the 2 weeks using KYN. Although decreases in the feelings of muscle tension, pain and difficulty in movement of the shoulder joint in group A, and decreases in the feelings of muscle tension, pain, and muscle hardness in group C were found, VAS of overall symptoms and other individual parameters did not significantly change. In conclusion, repeated treatments with KYN are thought to be more effective than conventional remedies. The differences in the effects of the treatment with KYN are ascribed to the etiology and symptoms that led to the subjects' initial remedies. Further studies are necessary to enhance these beneficial effects on shoulder-neck pain.

Implementing Evidence-Based Physiotherapy Practice for Treating Children with Low Back Pain: Are We There Yet?

This article reports on the process used to appraise evidence-based physiotherapy practice when treating nonspecific low back pain (LBP) in children and adolescents. Best clinical practices were identified using 9 systematic reviews reporting on effectiveness of physiotherapy for individuals with nonspecific LBP. Fifty medical charts were reviewed to appraise practice. All literature reviews reported solely on adult treatment, none included children or adolescents. Strong evidence supported the effectiveness of therapeutic exercises and education. There was insufficient evidence to support the effectiveness of thermotherapy, transcutaneous electrical nerve stimulation, or ultrasound. Chart reviews indicated therapeutic exercises were prescribed for all 50 pediatric patients and postural education was provided to 48 patients. Eleven patients received thermotherapy, three received transcutaneous electrical nerve stimulation, and none was treated with ultrasound. Published literature reviews enabled physiotherapists to appraise evidence-based practices. Clinical guidelines for treating nonspecific LBP in children and adolescents are needed.

Bakera, a herbal steam bath for postnatal care in Minahasa (Indonesia): Documentation of the plants used and assessment of the method

Bakera, a steam bath prepared with various plants, is a traditional method used in Minahasa (North Sulawesi, Indonesia) for recuperation after childbirth. Semi-structured interviews were conducted with 14 midwives and 166 mothers in different villages of Minahasa, in order to document the preparation and plants used for the bakera. Overall, the use of 60 different plant species for the bakera was recorded. Among the 10 most frequently cited bakera plants, eight are essential oil plants, which are usually cultivated in the kitchen gardens and often used as spices. The therapeutic effects of thermotherapy and aromatherapy contribute to the effectiveness of the bakera. Thermotherapy soothes symptoms such as muscular strain, heaviness in the limbs, edema, loss of appetite, and constipation, whereas the essential oils of the plants used have an immuno-stimulant, antiseptic, and antiphlogistic effect. If applied under the guidance of an experienced person, during the late childbed, the bakera can be regarded as an effective and safe method for recuperation after child birth.

Molecular biology mechanisms and technologies of hypothermia

The molecular biology mechanisms of thermotherapy and its relations with oncogenes are reviewed retrospectively in this article.The technologies and procedures of clinical practice,the effects of thermotherapy combined with chemotherapy and radiotherapy are discussed too.

Effect of Thermotherapy on Elimination of Apple Stem Grooving Virus and Apple Chlorotic Leaf Spot Virus for In vitro-cultured Pear Shoot Tips

Apple stem grooving virus (ASGV) and apple chlorotic leaf spot virus (ACLSV) are two major viruses of pear. In this study, in vitro thermotherapy was carried out at 37°C for 25, 30 and 35 days followed by subculturing of meristem tips of different sizes to eliminate ASGV and ACLSV from pear plants. Virus titers in heat-treated shoot tips were evaluated by ELISA testing of regenerated plants. Results showed that thermotherapy for 35 days significantly decreased the titer of ASGV and ACLSV in cultures regenerated from tips of main and axillary shoots, especially in those from explants 1 mm in length from the tip of meristems. Dot-blot hybridization of biotinylated cDNA probes derived from ACLSV and ASGV was used to detect these viruses in crude tissue extracts of in vitro-grown pear plants. Intense signals were consistently detected in untreated plant samples equivalent to less than 0.5 mg tissue. Comparison of signals from dot-blot hybridization and ELISA absorbance values (A405) confirmed that dot-blot hybridization had a higher sensitivity than PAS-ELISA. Dot-blot hybridization could detect viruses with a titer below the threshold level of ELISA. These results indicate that dot-blot hybridization is a useful tool for large-scale surveys of viruses, which facilitates the production of virus-free propagation materials in certification and sanitation programs. Results of PAS-ELISA and dot-blot hybridization showed that high virus elimination efficiency was achieved by a combination of thermotherapy for 35 days and in vitro culture of 1 mm meristem tips.