Effect Of Strain Research Articles

Effect of bacterial fermentation on the ability of myofibrillar proteins to bind esters and its potential mechanism: Based on protein metabolism and structural changes

The effect of fermentation strains (Lactiplantibacillus plantarum CQ01107 and Staphylococcus simulans CD207) on the binding properties of porcine myofibrillar proteins (MPs) to esters was investigated from two perspectives: metabolism degree and structural alterations. Results demonstrated that S. simulans could reduce the particle size and α-helix content of MPs, while simultaneously increasing the absolute zeta potential and active sulfhydryl content. This process decreased protein aggregation and facilitated the unfolding of MPs, thereby enhancing their binding to esters. Conversely, L. plantarum showed limited promotion, which might be related to its robust acid production and protein hydrolysis capacities. In addition, ethyl octanoate, with a longer carbon chain length, was found to have the highest binding capacity to MPs (28.38 %–41.59 %). Molecular docking results further revealed that the binding of the four esters to MPs was spontaneous, with ethyl octanoate exhibiting the lowest binding energy to MPs (−5.635 kcal/mol). The primary forces involved in the binding of the four selected esters to MPs were hydrophobic interactions, hydrogen bonding, and van der Waals forces. These findings can provide new insights into the mechanisms by which fermentation strains influence flavor formation in fermented foods.

Comparative study of the strain and strain rate hardening mechanisms and its workability during hot deformation of TC4 titanium alloys

Localized thinning is one of the main defects in hot forming processes, which is determined by the hardening behaviour during deformation. This study focused on building a hot-workability map to prevent localized thinning in wide ranges (650–950 °C & 0.001–1 s−1) for industrial significance. Insights into the associated strain and strain rate hardening mechanisms were provided by visualizing the microstructure evolutions using both in-situ High-Temperature Laser Scanning Confocal Microscope (HTLSCM) and Electron Backscattered Diffraction (EBSD). Results show that the highest uniform strain, up to 0.250, is achieved in the viscoplastic region due to the cooperative strain and strain rate hardening, where dislocation slip, α/β transformation and grain sliding concurrently occur. Significant grain refinement is also achieved. The optimum forming condition was identified in the viscoplastic region and is further verified by forming an ultra-thin large bend component, reducing thickness unevenness from 18.6 % to 13.1 %. This research finding could aid in understanding the effects of strain and strain rate hardening on forming uniformity and further provide scientific guidance for industrial processing parameters selection, reducing localized thinning and hence scrap rates.

Development of Johnson-Cook-Distinct Lattice Spring Model and its application in projectile penetration into metal targets

Projectile penetration into metal targets plays an important role in modern protective structure engineering, damage assessment in terrorist attacks, and car clash accident analysis, etc. The penetration process of metal target has been characterized by large deformation, high temperature, high pressure, and dynamic damage, which can be classified as a continuous-discontinuous dynamical process. To capture the dynamic responses of metal materials subjected to high-speed penetration, Johnson-Cook model has been implemented in a continuum-discrete model, namely, Distinct Lattice Spring Model (DLSM). This is achieved by redefining the constitutive model in DLSM, with plasticity hardening (plastic strain effects), strain rate, temperature, damage evolution, equation of state, etc., being taken into account, thus developing a new numerical model called JC-DLSM model. This model is validated through studying Taylor rod high-speed impact experiments, thin metal plate penetration experiments, and projectile impact experiments on titanium alloy targets. Good agreement between numerical modelling and experimental data has been achieved for all cases considered, thereby demonstrating the capability of JC-DLSM to reproduce the damage characteristics and ballistic limits of metals under high-speed impact/penetration. Then, the impacts of projectile shape, metal target surface configuration on the characteristics of penetration damage patterns have been investigated. This contributes to a better understanding of the damage mechanisms of metal targets upon penetration or impact, facilitating the computational means for analysing and optimizing the penetration resistance of protective engineering structures.

Biaxial strain effect on Josephson supercurrent in a 2D Weyl semimetal

Biaxial strain effect on Josephson supercurrent in a 2D Weyl semimetal

Investigation of the Physical Properties through Strain Effect of Monolayer Silicon Carbide Material: DFT Analysis

Investigation of the Physical Properties through Strain Effect of Monolayer Silicon Carbide Material: DFT Analysis

Controllable magnetic anisotropy in two-dimensional 1T-CrTe2 with electrides sublayer

Exploring the controlled magnetic anisotropy energy (MAE) in two-dimensional (2D) ferromagnets is an essential step towards the emergent magnetic tunnel junctions (MTJs) with robust storage stability and low-power consumption. In addition to the transitional charge doping method, we propose that stacking 2D ferromagnet 1T-CrTe2 with electrides substrate can achieve not only the high interfacial charge transfer up to 5.24×1014 cm−2 and but also efficient modification of magnetic behaviors via interfacial engineering. Employing first-principles calculations, we show that the 1T-CrTe2/Ca2N(Y2C) heterostructures exhibit a significant reduction in MAE with a spin reorientation. Notably, the synergistic effect of internal charge transfer, external strain and charge doping shows a significant influence on the magnetic behaviors of the bilayer structures, enabling an efficient modulating of their MAE with distinct dependences. We elucidate that the underlying mechanism is the synergistic effect induced alteration of the spin-polarized px and py states on the Te atom located at the interfaces, which in turn changes the competitive spin-orbit coupling (SOC) contributions to the MAE. These findings provide a practical path toward the controllable MAE in 2D ferromagnets, and make the proposed heterostructures promising candidates for emergent spintronic devices.

Evaluation of Lactobacillus Sakei Probiotic Supernatant’s Effectiveness in Promoting Apoptosis and Decreasing Breast Cancer MCF-7 Cancerous Cells through Modulation of Bax and Bcl2 Genes

Introduction: Accumulating evidence has revealed that inducing apoptosis is an important strategy to control excessive breast cancer cell proliferation. In this study, the supernatant effect of the probiotic strain of Lactobacillus Sakei on Expression Level of Apoptosis-Related Genes (Bax/Bcl2) in Breast Cancer Cell Line (MCF-7) was investigated. Methods: In the experimental study, MCF-7 breast cancer cells were cultured in DMEM medium with 10% bovine serum. The cells were treated in 1, 2, 3, 4 and 5 mg/ml concentrations of sakei supernatant and incubated at 24, 48 and 72 hours. The MTT assay was used to measure cytotoxicity effect according to kit manufacturer's protocol in all three incubation times. MCF-7 cells with concentration of sakei supernatant at IC50 point (5 mg / ml) to examine the expression of genes Bax and Bcl2 were incubated for 72 hours. Real-Time PCR was performed to analyze the changes in the expression of Bax and Bcl2 genes. Results: The results of this study indicated that bioavailability of MCF-7 cell line reached the lowest value at concentration of 5 mg/ml compared to the control group. In addition, MCF-7 cell line treatment with Lactobacillus sakei supernatant at a dose of 5 mg/ml and 72 h incubation time, showed significantly increased expression of BAX (p-value= 0.0033) and significantly decreased expression of BCL-2 (p-value= 0.0278). Conclusion: The results indicate that Lactobacillus sakei supernatant can reduce the bioavailability of breast cancer cells by inducing apoptosis pathway.

Endorsing Deviance: Examining the Influence of Legitimacy and Injustice on Responses to Strain

ABSTRACT Although scholars have theoretically addressed how information from others can affect justice processes, little empirical research investigates how individuals make sense of a distributive injustice when also attending to other justice and legitimacy concerns. This research examines how legitimacy conditions emotional and behavioral responses to various forms of injustice associated with strain. Drawing from General Strain Theory (GST) and the social psychological literature on legitimacy and justice, I investigate how endorsement of a deviant response to injustice, while failing to support the perpetrator of injustice, influences negative affect and projected deviant coping in response to a distributively unjust blocked goal. A randomized vignette survey was distributed to a sample of undergraduates. Results reveal that legitimacy affects perceptions of justice and conditions the effect of unjust strain on negative affect and the likelihood of deviance, but only when certain types of injustice are present.

Sequential Immune Acquisition of Monoclonal Antibodies Enhances Phagocytosis of Acinetobacter baumannii by Recognizing ATP Synthase

Objectives: The aim of this study was to prepare monoclonal antibodies (mAbs) that broadly target Acinetobacter baumannii and protect against infection by multi-drug-resistant (MDR) A. baumannii from different sources. Methods: mAb 8E6 and mAb 1B5 were prepared by sequentially immunizing mice with a sublethal inoculation of three heterogeneous serotypes of pan-drug-resistant (PDR) A. baumannii, ST-208, ST-195, and ST-229. Results: The cross-recognition of heterogeneous bacteria (n = 13) by two mAbs and potential targets was verified, and the in vitro antibacterial efficacy of mAbs was assessed. The median killing rate of mAb 8E6 against A. baumannii in the presence of complement and dHL-60 cells was found to be 61.51%, while that of mAb 1B5 was 41.96%. When only dHL-60 cells were present, the killing rate of mAb 8E6 was 65.73%, while that of mAb 1B5 was 69.93%. We found that mAb 8E6 and mAb 1B5 broadly targeted MDR A. baumannii on the ATP synthase complex and were equipped with an antibacterial killing ability by enhancing the innate immune bacteriolytic effect of ST-208 and ST-195 strains. Both monoclonal antibodies were validated to protect against respiratory infection at 4 and 24 h via enhancing the release of innate immune substances and inflammatory cytokines, effectively shortening the disease period in mice. Conclusions: mAb 8E6 and mAb 1B5 significantly enhanced the opsonization process of phagocytosis against A. baumannii strains prevalent in southern China by targeting ATP synthase antigens thereof, resulting in protective effects in mice.

Akkermansia muciniphila improve cognitive dysfunction by regulating BDNF and serotonin pathway in gut-liver-brain axis

BackrgroundAkkermansia muciniphila, a next-generation probiotic, is known as a cornerstone regulating the gut-organ axis in various diseases, but the underlying mechanism remains poorly understood. Here, we revealed the neuronal and antifibrotic effects of A. muciniphila on the gut-liver-brain axis in liver injury.ResultsTo investigate neurologic dysfunction and characteristic gut microbiotas, we performed a cirrhosis cohort (154 patients with or without hepatic encephalopathy) and a community cognition cohort (80 participants in one region for three years) and validated the existence of cognitive impairment in a 3,5-diethoxycarbonyl-1,4-dihydrocollidine-induced hepatic injury mouse model. The effects of the candidate strain on cognition were evaluated in animal models of liver injury. The expression of brain-derived neurotrophic factor (BDNF) and serotonin receptors was accessed in patients with fibrosis (100 patients) according to the fibrosis grade and hepatic venous pressure gradient. The proportion of A. muciniphila decreased in populations with hepatic encephalopathy and cognitive dysfunction. Tissue staining techniques confirmed gut-liver-brain damage in liver injury, with drastic expression of BDNF and serotonin in the gut and brain. The administration of A. muciniphila significantly reduced tissue damage and improved cognitive dysfunction and the expression of BDNF and serotonin. Isolated vagus nerve staining showed a recovery of serotonin expression without affecting the dopamine pathway. Conversely, in liver tissue, the inhibition of injury through the suppression of serotonin receptor (5-hydroxytryptamine 2A and 2B) expression was confirmed. The severity of liver injury was correlated with the abundance of serotonin, BDNF, and A. muciniphila.ConclusionsA. muciniphila, a next-generation probiotic, is a therapeutic candidate for alleviating the symptoms of liver fibrosis and cognitive impairment.Graphical

Optical Properties of Multilayered Staggered SiGe Nanodots Depending on Si Spacer Growth Temperature

The optical properties of the multilayered staggered SiGe nanodots (NDs) embedded in the Si spacers fabricated at varying Si growth temperatures are clarified in conjunction with the effect of strain by Photoluminescence (PL) and Raman spectroscopy. We found that compressive strain is induced in the SiGe NDs, with higher growth temperatures of the Si spacer resulting in stronger compressive strain. PL spectra indicate that the higher growth temperature leads to a wider bandgap. This transition energy behavior is caused by not only the strain in the SiGe NDs but also the Ge segregation. Furthermore, luminescence from the SiGe NDs was observed along with luminescence originated at the Si/SiGe interface in the SiGe NDs with a small dot size. This phenomenon was caused by the small energy difference of the conduction-band minima between the SiGe NDs and the tensile-strained Si spacers and the small volume of the tensile-strained Si spacers.

Validation of the Mediating Effect of Parenting Stress in the Relationship between Work-Family Strains and Gains and Marital Satisfaction for Fathers in Korea.

This study aimed to examine the relationship between Korean fathers' work-family strains and gains and marital satisfaction and to validate the mediating effect of parenting stress in the relationship. This study used the 14th-year survey data of the Panel Study of Korean Children (PSKC) conducted by the Korea Institute of Child Care and Education (KICCE), the latest available data. The PSKC is a longitudinal study of children's growth and development from birth in the Republic of Korea. After excluding datasets containing missing values from the data collected from 2150 respondents, the data were extracted from the survey responses collected from 1130 respondents, and descriptive statistical analysis, path analysis, and mediating effect analysis were conducted. First, Korean fathers' work-family strains had a positive (+) influence on parenting stress (β = 0.658, p < 0.001). Second, Korean fathers' work-family strains had a negative (-) influence on marital satisfaction (β = -0.185, p < 0.001). Third, Korean fathers' work-family gains did not have a statistically significant influence on parenting stress (β = 0.016, p = 0.632). Fourth, Korean fathers' work-family gains had a positive (+) influence on marital satisfaction (β = 0.381, p < 0.001). Fifth, Korean fathers' parenting stress did not have a statistically significant influence on marital satisfaction (β = 0.011, p = 0.783). Sixth, the indirect effect of work-family strains on marital satisfaction via parenting stress (β = 0.013, 95% Bias-corrected CI = -0.042∼0.075) was statistically significant, and specifically, it had a partial mediating effect. Seventh, the indirect effect of work-family gains on marital satisfaction via parenting stress (β = 0.028, 95% Bias-corrected CI = 0.005∼0.051) was statistically significant, and specifically, it had a partial mediating effect. This study aims to provide the basic data for various family support policies for fathers by understanding the current status of work-family balance for fathers in modern society, as well as by examining the effects of parenting stress in the relationship with marital satisfaction.

Quality of life, capability well-being, financial strain and physical activity in the short- and medium-term COVID-19 post-lockdown phases in the UK: a repeated cross-sectional study.

The COVID-19 lockdowns had negative effects on children's and adults' mental and physical health. There is, however, a paucity of research that explores differences in health-related quality of life (HRQL) and well-being over time after the COVID-19 lockdowns had been lifted. Furlough during lockdowns, increases in unemployment, and the emerging cost-of-living crisis all put pressure on family finances, which could have a detrimental effect on HRQL and well-being. This study, part of the wider Active-6 study, explored how HRQL, capability well-being and family financial strain changed after the lockdowns, the relationship between these outcomes, and whether physical activity had any mediating effect on differences in HRQL and capability well-being. Cross-sectional data were collected in May-December 2021 (Wave 1) and January-July 2022 (Wave 2). Children (aged 10-11) and their parent/carer were recruited from 23 to 27 schools in each wave, respectively, and completed validated questionnaires measuring HRQL (adults - EQ-5D-5L, children - CHU9D), capability well-being (adults - ICECAP-A) and family financial strain (adults - Family Economic Strain Scale, FESS). Children also completed questions on capability well-being. Weekday minutes of moderate-to-vigorous physical activity (MVPA) were measured using accelerometers. Mixed-effects regression models, adjusted for gender, age group (adults only), IMD and highest household education, were used to explore differences in HRQL and capability well-being between waves. In addition, the moderating effect of financial strain and the mediating effect of MVPA on HRQL and capability well-being were explored. Active-6 recruited 393 parent-child pairs in Wave 1 and 436 in Wave 2. There were no differences in HRQL (EQ-5D, CHU9D) and capability well-being (ICECAP-A) scores between waves, but financial strain was worse in Wave 2 compared to Wave 1 (FESS score difference 1.14 adjusted 95% CI 0.15 to 2.12). Increased financial strain was associated with lower (worse) EQ-5D-5L, CHU9D and ICECAP-A scores. There was no evidence of a mediating effect of MVPA. Pre-COVID-19 data on HRQL were not collected, so analysis was limited to post-lockdown only. Participating parents were predominantly female and participation was lower among lower socio-economic groups, limiting our ability to explore inequalities. Intervention planning to increase physical activity and health and well-being during the COVID-19 recovery should consider the financial strain families are experiencing and the negative implications of financial strain on HRQL. There were no differences in HRQL and capability well-being in children and adults after lockdowns lifted in 2021 and a year later in 2022. The results indicate increasing financial strain, which could reflect the UK's 'cost of living crisis'. This article presents independent research funded by the National Institute for Health and Care Research (NIHR) Public Health Research programme as award number NIHR131847.

Setting of seeds during Agrobacterium-mediated winter wheat transformation by in planta method

Aim. To investigate the frequency of seeds setting and the formation of transgenic plants during Agrobacterium-mediated transformation of winter wheat by the in planta method using different methods of applying agrobacteria and the composition of inoculation media. Methods. Agrobacterium-mediated transformation by in planta method, molecular genetic analysis, methods of mathematical statistics. Results. A significant difference was found in the studied genotypes of winter wheat according to the indicator seed setting depending on the A. tumefaciens strain used and the inoculation medium. When comparing the effect of different strains of agrobacteria on this indicator, no significant differences between them were established, however, a tendency to increase the frequency of seed formation was observed when using the LBA4404 strain. When using the MS-22 inoculation medium, which additionally contained 12.5 mM MES, 4 mM NH4CI, 5.5 mM MgSO4, a certain increase in the number of formed seeds was also observed compared to the MS-21 medium supplemented with sodium thiosulfate, the frequency of seed setting increased in on average by 5 %. Conclusions. The genotypic dependence of the frequency of seed setting and the formation of transgenic plants with the use of different inoculation media and the method of the transformation procedure was established.

Carcass Characteristics and Blood Biochemical Parameters of Cobb-500 and Hubbard Chicken Strains Fed on Commercial and Farm-Formulated Diets

The limits of commercial diets, their quality, and their rising costs are some of the major challenges to broiler production in Ethiopia. The purpose of this investigation was to evaluate carcass yield characteristics and blood biochemical parameters of Cobb-500 and Hubbard chicken strains fed on farm-formulated diets (T1) and three different commercial diets (T2, T3, and T4). A total of 384 mixed-sex day-old chicks (192 per strain) were randomly assigned to four dietary treatments with four replicates, each consisting of 12 broilers. The experiment was set up as a 2 × 4 factorial design, providing each strain with four diets in a completely randomized design. After 42 days of the experiment, one male and one female of each strain from each pen (eight birds per treatment) were slaughtered for carcass yield and hematological analysis. Although diets had a significant impact on live body weight, feed conversion ratio, and feed consumption among the study treatments, they had no significant effect on the mortality rate of the broilers as a whole. There was a significant effect of strains on the weight of eviscerate, dress, thigh, drumstick, breast, neck, back, and eviscerate yield percentage, with Cobb 500 showing higher values than Hubbard broilers. The farm-formulated diet (T1) significantly increased the weight of non-edible offal compared to the commercial diets, except for the weight of crops and lungs, which were similar to those in commercial diet group T4. The Hubbard strain showed a higher least square mean for packed cell volume than the Cobb-500 strain. Sex was found to have no significant impact on the hematological parameters. The farm-formulated diet (T1) also resulted in a higher marginal return rate than that of the commercial diet (T3) in the Cobb-500 strain. These findings suggest that locally sourced farm-formulated diets could be a viable alternative to commercial diets for broiler chickens in the study area. Keywords: Broiler chicken, biochemical parameter, Carcass trait, Farm-made diet, Haematology, Profitable

First-principles study of optical aspects of Penta-graphene monolayer under strain effects

First-principles study of optical aspects of Penta-graphene monolayer under strain effects

Tunable Out-of-Plane Reconstructions in Moiré Superlattices of Transition Metal Dichalcogenide Heterobilayers.

The reconstructed moiré superlattices of the transition metal chalcogenide (TMD), formed by the combined effects of interlayer coupling and intralayer strain, provide a platform for exploring quantum physics. Here, using scanning tunneling microscopy/spectroscopy, we observe that the strained WSe2/WS2 moiré superlattices undergo various out-of-plane atomically buckled configurations, a phenomenon termed out-of-plane reconstruction. This evolution is attributed to the differentiated response of intralayer strain in high-symmetry stacking regions to external strain. Notably, in larger out-of-plane reconstructions, there is a significant alteration in the local density of states (LDOS) near the Γ point in the valence band, exceeding 300%, with the moiré potential in the valence band surpassing 200 meV. Further, we confirm that the variation in interlayer coupling within high-symmetry stacking regions is the main factor affecting the moiré electronic states rather than the intralayer strain. Our study unveils intrinsic regulating mechanisms of out-of-plane reconstructed moiré superlattices and contributes to the study of reconstructed moiré physics.

Edge sites regulation, strain and electric field effect on MoS2/CoS2 heterojunction catalysts for hydrogen evolution reaction.

Heterojunction catalysts in the field of hydrogen evolution reaction (HER) from electrocatalytic water splitting have recently become a hot research topic. In this paper, we systematically calculated the HER catalytic performance of a MoS2/CoS2 heterojunction for the first time, considering the effect of edge sites regulation, strain and electric field. The results indicate that the MoS2/CoS2 heterojunction exhibits synergistic catalytic performance compared to MoS2 and CoS2, the HER catalytic activity of which can be improved by exposing more edge sites or regulating the S content on the edges, with an optimized ratio of 25%. Surprisingly, applying strain has a slight effect on the catalytic activity of the edge, however, an obvious effect on the basal plane. For example, applying 2% tensile strain on the MoS2/CoS2 heterojunction can improve the edge catalytic performance by 13%, and for the basal plane, this value can reach 92%. In this case, the catalytic performance of the basal plane is better than that of the edge with 2% and without strain. Since the basal plane accounts for the majority of the two-dimensional catalysts, the catalytic performance of the basal plane is generally much lower than that of the edge. This discovery is of great significance, which means by adjusting strain, the catalytic performance of the heterojunction catalyst is likely to be improved by orders of magnitude. Moreover, considering the actual experimental process, we also calculated the effect of the electric field and found that 0.7 V/Å electric field can enhance the HER catalytic activity of the MoS2/CoS2 heterojunction by 23%.

Organic-inorganic coupled Ti3CN MXene achieving the excellent sodium-ion storage performance

Two-dimensional (2D) transition metal carbides/nitrides (MXenes) are attracting considerable attention due to their unique multilayered structure. However, the practical application of MXenes as electrode materials is hampered by sluggish ions diffusion kinetics and serious volume variation during charging and discharging cycles. Herein, the organic-inorganic coupled Ti3CN (Ti3CNNHSA) composites were constructed by anchoring succinic acid (SA) molecules into the interlayer of Ti3CN. The anchored SA molecules contribute pillar effects to increase interlayer spacing and inhibit the volume contraction during Na+ ions extraction processes, as well as strain effects to mitigate volume expansion during Na+ ions insertion processes. Consequently, the Ti3CNNHSA composites not only exhibit the increased interlayer spacing and rapid ions transfer paths but also deliver the enhanced structural stability and maximum utilization of layered structure, realizing extraordinary cycle stability and high rate capability. The Ti3CNNHSA exhibits a superior capacity retention of 95.2% after 400 cycles at 0.1 A g-1. The Ti3CNNHSA//AC sodium-ion capacitor (SIC) also displays a capacity retention of 84.1% after 1200 cycles at 1.0 A g-1 and a high energy density of 76 Wh kg-1 at the power density of 200 W kg-1. Such an organic-inorganic coupled Ti3CNNHSA sheds light on the development of 2D layered materials.

Gambling disorder gender analysis: social strain, gender norms, and self-control as risk factors.

Gender differences in problem gambling have attracted much attention in recent gambling literature. However, relatively little is known about how gender norms relate to social strain and self-control in predicting gambling disorder within a spousal context. This study aimed to increase knowledge about gambling disorder in Chinese married couples by assessing the three-way interaction effects between social strain, self-control, and gender norms. A total of 1,620 Chinese married couples were recruited from a representative sample of households in Hong Kong. The results of the generalized ordered logit model revealed the self-control mitigation effect of composite strain on the propensity for gambling disorder is strong in men who accept traditional gender norms. In contrast, in women who accept traditional gender roles, self-control attenuates the effect of recent stressful life events on the propensity for gambling disorder, but self-control exacerbates the effect of negative relationships with offspring on the propensity for gambling disorder. Although reinforcing self-control is a protective factor that can alleviate social strain and disordered gambling for both men and women, the prominent contribution of gender norms to the self-control exacerbation effect deserves close attention for social workers who provide services to these gambling families.