Effects Of Short-term Exposure Research Articles

Efekt krótkotrwałej ekspozycji na pył zawieszony (PM2,5 oraz PM10) oraz jego wpływ na nagłe zaostrzenia wybranych chorób układu krążenia i oddechowego we Wrocławiu w latach 2009–2013 na podstawie rejestru wyjazdów Pogotowia Ratunkowego

1. Krzeszowiak J, Pawlas K. Zanieczyszczenie powietrza we Wrocławiu i potencjalne zagrożenie dla zdrowia z tym związane. Med Srod. 2015; 18(2): 66–73. WWW Google Scholar

Short-term exposure to sulphur dioxide (SO2) and all-cause and respiratory mortality: A systematic review and meta-analysis

BackgroundMany studies have assessed the harmful effects of ambient air pollution on human mortality, but the evidence needs further exploration, analysis, and refinement, given the large number of studies that have been published in recent years. The objective of this study was to evaluate all the available evidence of the effect of short-term exposure to ambient sulphur dioxide (SO2) on all-cause and respiratory mortality. MethodsArticles reporting observational epidemiological studies were included, comprising time-series and case-crossover designs. A broad search and wide inclusion criteria were considered, encompassing international and regional databases, with no geographical or language restrictions. A random effect meta-analysis was conducted, and pooled relative risk for an increment of 10 µg/m3 in SO2 concentrations were calculated for each outcome. We analysed the risk of bias (RoB) in individual studies for specific domains using a new domain-based RoB assessment tool, and the certainty of evidence across studies with an adaptation of the Grading of Recommendations Assessment, Development and Evaluation approach. The certainty of evidence was judged separately for each exposure-outcome combination. A number of subgroup and sensitivity analyses were carried out, as well as assessments of heterogeneity and potential publication bias. The protocol for this review was registered with PROSPERO (CRD42019120738). ResultsOur search retrieved 1,128 articles, from which 67 were included in quantitative analysis. The RoB was low or moderate in the majority of articles and domains. An increment of 10 µg/m3 in SO2 (24-hour average) was associated with all-cause mortality (RR: 1.0059; 95% CI: 1.0046–1.0071; p-value: <0.01), and respiratory mortality (RR: 1.0067; 95% CI: 1.0025–1.0109; p-value: <0.01), while the same increment in SO2 (1-hour max.) was associated with respiratory mortality (RR:1.0052; 95% CI: 1.0013–1.0091; p-value: 0.03). Similarly, the association was positive but non-significant for SO2 (1-hour max.) and all-cause mortality (RR: 1.0016; 95% CI: 0.9930–1.0102; p-value: 0.60). These associations were still significant after the adjustment for particulate matter, but not for other pollutants, according to the results from 13 articles that evaluated co-pollutant models. In general, linear concentration–response functions with no thresholds were found for the two outcomes, although this was only evaluated in a small number of studies. We found signs of heterogeneity for SO2 (24-hour average) – respiratory mortality and SO2 (1-hour max.) – all-cause mortality, and funnel plot asymmetry for SO2 (24-hour average) – all-cause mortality. The certainty of evidence was high in two combinations, i.e. SO2 (24-hour average) – all-cause mortality and SO2 (1-hour max.) – respiratory mortality, moderate in one combination, i.e. SO2 (24-hour average) – respiratory mortality, and low in the remaining one combination. ConclusionsPositive associations were found between short-term exposure to ambient SO2 and all-cause and respiratory mortality. These associations were robust against several sensitivity analyses, and were judged to be of moderate or high certainty in three of the four exposure-outcome combinations.

Acute effects of short-term exposure to ambient air pollution on reproductive hormones in young males of the MARHCS study in China

Air pollution, which is associated with male reproductive health. However, it is unknown the acute effects of ambient air pollutants exposure on male reproductive hormones. The current study, we measured serum levels of reproductive hormone in 2030 blood samples gathered from The Male Reproductive Health in Chongqing College Students (MARHCS) cohort study. We derived a full coverage of ambient air pollutant (PM10, PM2.5, SO2, NO2, CO and O3) concentrations by employing machine learning algorithms, and used a mixed-effect model to estimate single-day and cumulative effects of air pollutants exposure on serum reproductive hormones. Our results showed that (1) PM10 and PM2.5 concentrations were positively associated with estradiol (E2) in both single and cumulative lag days, but were negatively associated with the ratio of Testosterone/E2 (the T/E2 ratio). NO2 was positively associated with estradiol at lag day 2 (95% CI: 0.290, 0.881; corrected P = 0.048) and lag 0–2 days (95% CI: 0.523, 1.337; corrected P = 0.003), with progesterone (P) at lag day 2 and lag day 3 (corrected P < 0.05). There was also a positive association between CO exposure and progesterone at lag day 2. (2) SO2 was inversely associated with E2 at lag day 3, 4 and lag 0–4 days, and progesterone at lag day 0, 1, 2 and lag 0–1, 0–2, 0–4 days, but positively associated with the T/E2 ratio at lag day 3, 4 and lag 0–4 days (corrected P < 0.05). O3 exposure was negatively associated with E2 at lag day 3 (95% CI: −0.216, −0.074, corrected P = 0.03). (3) No significant associations were found between the cumulative daily average air pollutant exposure of CO, O3 and hormone outcomes. This study suggests that short-term exposure to air pollutants may thus alter reproductive hormone levels, especially on serum estradiol, progesterone levels and the T/E2 ratio.

Exposure to Secondhand Heated-Tobacco-Product Aerosol May Cause Similar Incidence of Asthma Attack and Chest Pain to Secondhand Cigarette Exposure: The JASTIS 2019 Study.

Although secondhand cigarette smoke is known to cause various health consequences, even the short-term effects of exposure to secondhand heated-tobacco-product (HTP) aerosol are unknown. The purpose of this study was to examine short-term symptoms related to secondhand HTP aerosol exposure. An internet-based self-reported questionnaire survey was conducted in 2019 as a part of the Japan Society and New Tobacco Internet Survey (JASTIS) study. In total, 8784 eligible respondents aged 15–73 years were analyzed. We examined the frequency (%) of secondhand combustible cigarette smoke and HTP aerosol exposure, and the exposure-related subjective symptoms (sore throat, cough, asthma attack, chest pain, eye pain, nausea, headache, and other symptoms). Overall, 56.8% of those exposed to secondhand cigarette smoke had any subjective symptoms, compared to 39.5% of those exposed to HTP aerosol. Asthma attack and chest pain were reported more frequently when associated with secondhand HTP exposure (10.9 and 11.8%, respectively) than with secondhand cigarette smoke exposure (8.4 and 9.9%, respectively). Sore throat, cough, eye pain, nausea, and headache were also more frequently reported when associated with secondhand cigarette smoke than with secondhand HTP exposure. This is the first study to examine severe subjective symptoms such as asthma attacks and chest pains, and to suggest that respiratory and cardiovascular abnormalities could be related to secondhand heated-tobacco-product aerosol exposure. Further careful investigations are necessary.

Toxicity assessment of gold ions and gold nanoparticles to golden perch larvae (Macquaria ambigua)

AbstractGolden perch (Macquaria ambigua) is a freshwater game-fish native to central and southeast Australia. Larvae of this fish species were used in two different types of experiments to evaluate the effects of short-term exposures (up to 6 days) to aqueous gold, 5 nm gold nanoparticles (AuNPs), or 50 nm AuNPs. Relative to the control, increased gold concentrations corresponded with yolk-sac edema (swelling). Larvae exposed to 50 μM of 5 nm AuNPs had yolk-sacs that were ~1.5 times larger resulting in the appearance of bent notochords. After two days of exposure, 100% mortality was observed. Total mortalities were &lt;25% in the other larvae–gold systems, suggesting that these larvae can quickly adapt to the presence of gold. In terms of an oxidative stress response, the larvae from all systems did not express high enzymatic activity. The state of the gold determined how much could be taken up (or immobilised) by a larva. Aqueous gold and 5 nm AuNPs easily pass through cells; therefore, larvae exposed to these forms of gold contained the highest concentrations. Scanning electron microscopy confirmed that cells comprising the epithelium and fins contained AuNPs. Aqueous gold was reduced to nanometre-scale particles within cells. Comparatively, 5 nm AuNPs appeared to be aggregated within cells forming clusters hundreds of nanometres in size. On the contrary, 50 nm AuNPs were not observed within cells but were detected within larvae by (single particle) inductively coupled plasma mass spectroscopy, suggesting that these AuNPs were probably taken up through the mouth or gills. The results of the present study demonstrate that exposure to AuNPs had adverse effects on developing golden perch larvae. Additionally, these effects were dependent on the size of the AuNPs.

Elevated CO2 differentially regulates root nitrate transporter kinetics in a genotype and nitrate dose-dependent manner

The nitrogen (N) and protein concentration of wheat crop and grain often decline as a result of exposure of the crop to elevated CO2 (EC). In our earlier studies, it was found that the exacerbated production of nitric oxide (NO) represses the transcription of nitrate reductase (NR) and high affinity nitrate transporters (HATS) in EC grown wheat seedlings receiving high N. High N supply under EC also resulted in accumulation of reactive oxygen species (ROS), and reactive nitrogen species (RNS; NO and S- nitrosothiols) ensuing faster senescence and reduced N metabolite concentration in wheat. In this study, the effect of short-term exposure to EC on nitrate uptake kinetics was studied. The impact of EC on constitutive and inducible components of high affinity and low affinity nitrate uptake systems (HATS and LATS) were delineated in two wheat genotypes diverse in terms of nitrate uptake and assimilation capacities. Nitrate dose-response of NR was suppressed by EC in both leaf and root tissues. Plants grown under EC displayed a marked reduction in nitrate uptake kinetic components of LATS. Wheat genotype with high leaf nitrate assimilation capacity was able to maintain considerably higher nitrate uptake rate under EC albeit at a lower rate in comparison to ambient CO2. Wheat leaves exposed to EC displayed a comparatively higher abundance of NO and showed incremental abundance depending on increase in nitrate supply. Exogenous NO supply significantly suppressed the nitrate uptake rate of EC grown plants. Hence, EC-induced production of NO downregulates LATS kinetics in a genotype and nitrate dose-dependent manner.

Effects of short-term exposure to ambient airborne pollutants on COPD-related mortality among the elderly residents of Chengdu city in Southwest China

BackgroundChronic obstructive pulmonary disease (COPD) has become a severe global burden in terms of both health and the economy. Few studies, however, have thoroughly assessed the influence of air pollution on COPD-related mortality among elderly people in developing areas in the hinterland of southwestern China. This study is the first to examine the association between short-term exposure to ambient airborne pollutants and COPD-related mortality among elderly people in the central Sichuan Basin of southwestern China.MethodsData on COPD-related mortality among elderly people aged 60 and older were obtained from the Population Death Information Registration and Management System (PDIRMS). Data on airborne pollutants comprised of particulate matter < 2.5 μm in aerodynamic diameter (PM2.5), sulfur dioxide (SO2), nitrogen dioxide (NO2), carbon monoxide (CO), and ozone (O3) were derived from 23 municipal environmental monitoring sites. Data on weather conditions, including daily mean temperature and relative humidity, were obtained from the Chengdu Meteorological Bureau. All data were collected from January 1, 2015, to December 31, 2018. A quasi-Poisson general additive model (GAM) was utilized to assess the effects of short-term exposure to airborne pollutants on COPD-related mortality among elderly people.ResultsA total of 61,058 COPD-related deaths of people aged 60 and older were obtained. Controlling the influences of daily temperature and relative humidity, interquartile range (IQR) concentration increases of PM2.5 (43 μg/m3), SO2 (8 μg/m3), NO2 (18 μg/m3), CO (0.4 mg/m3), and O3 (78 μg/m3) were associated with 2.7% (95% CI 1.0–4.4%), 4.3% (95% CI 2.1–6.4%), 3.6% (95% CI 1.7–5.6%), 2.7% (95% CI 0.6–4.8%), and 7.4% (95% CI 3.6–11.3%) increases in COPD-related mortality in people aged 60 and older, respectively. The exposure-response curves between each pollutant and the log-relative risk of COPD-related mortality exhibited linear relationships. Statistically significant differences in the associations between pollutants and COPD-related mortality were not observed among sociodemographic factors including age, gender, and marital status. The effects of O3 remained steady after adjusting for PM2.5, SO2, NO2, and CO each time in the two-pollutant models.ConclusionsIncreased concentrations of ambient airborne pollutants composed of PM2.5, SO2, NO2, O3, and CO were significantly and positively associated with COPD-related mortality in the central Sichuan Basin, which is located in the hinterland of southwestern China. The adverse effects of O3 were stable, a finding that should receive more attention.

And Exposures and Lung Function in Swiss Adults: Estimated Effects of Short-Term Exposures and Long-Term Exposures with and without Adjustment for Short-Term Deviations.

Background:The impact of nitrogen dioxide () and particulate matter with an aerodynamic diameter of less than or equal to 2.5. microns () exposures on lung function has been investigated mainly in children and less in adults. Furthermore, it is unclear whether short-term deviations of air pollutant concentration need to be considered in long-term exposure models.Objectives:The aims of this study were to investigate the association between short-term air pollution exposure and lung function and to assess whether short-term deviations of air pollutant concentration should be integrated into long-term exposure models.Methods:Short-term (daily averages 0–7 d prior) and long-term (1- and 4-y means) and concentrations were modeled using satellite, land use, and meteorological data calibrated on ground measurements. Forced expiratory volume within the first second (FEV1) of forced exhalation and forced vital capacity (FVC) were measured during a LuftiBus assessment (2003–2012) and linked to exposure information from the Swiss National Cohort for 36,085 adults (ages 18–95 y). We used multiple linear regression to estimate adjusted associations, and additionally adjusted models of long-term exposures for short-term deviations in air pollutant concentrations.Results:A increase in and on the day of the pulmonary function test was associated with lower FEV1 and FVC (: FEV1 [95% confidence interval: , ], FVC [, ]; : FEV1 [, ], FVC [, ]). A increase in 1-y mean was also associated with lower FEV1 (; , 0.5) and FVC (; , ), as was a increase in 1-y mean (FEV1: ; , ; FVC: ; , ). These associations were robust to adjustment for short-term deviations in the concentration of each air pollutant.Conclusions:Short- and long-term air pollution exposures were negatively associated with lung function, in particular long-term exposure with FVC. Our findings contribute substantially to the evidence of adverse associations between air pollution and lung function in adults. https://doi.org/10.1289/EHP7529

Physiological and psychological effects of visits to different urban green and street environments in older people: A field experiment in a dense inner-city area

Urbanisation and global ageing are both accelerating, posing older people’s mental and cardiovascular health at risk. A growing number of studies suggests beneficial health effects when having contact with urban green spaces. This study investigates the effects of short-term exposure of older people to different urban green and street environments on physiological and psychological parameters relevant for cardiovascular health. A sample of 33 older men and women (mean age 63.5, standard deviation 4.2) was exposed to a long-standing urban park with widespread and old trees, to a newly developed park with diverse use opportunities and a busy street environment in the inner city of Leipzig, Germany. We measured heart rate variability using continuous ECG monitoring, blood pressure, mood using psychological questionnaires and perceived outcomes of restoration. We found significant decreases in systolic blood pressure and pulse pressure in the long-standing park indicating protective effects on cardiovascular health. Significant decreases in heart rate variability parameters were observed in the dense urban street environment indicating adverse effects on cardiovascular health. Level of naturalness and restoration experience were perceived highest in the old park reflecting common theories of restoration. In the light of these results, visiting urban green spaces should be considered as a valuable preventive measure for promoting cardiovascular health especially in older people. Although physical activity is considered by international recommendations for cardiovascular prevention, the combination with visits of green environments is yet to be implemented.

Lactobacillus Acidophilus and Lactobacillus Casei Exposed to Wi-Fi Radiofrequency Electromagnetic Radiation Show Enhanced Growth and Lactic Acid Production.

Background: Lactobacillus Acidophilus and Lactobacillus Casei are gram-positive probiotics and members of the genus Lactobacillus. These bacteria are widely applicable in food and dairy industries. Increasing bacterial load and decreasing fermentation time make them more profitable for manufacturers.Objective: This study was aimed at assessing the biological effects of short-term exposure of L. acidophilus and L. Casei to 2.4 GHz Wi-Fi radiofrequency electromagnetic fields (RF-EMF) generated by a Wi-Fi router on the lactic acid production and proliferation of these probiotic bacteria.Material and Methods: This experimental study was performed on pure culture strains of L. acidophilus and L. Casei, first direct vat sets (DVS) were activated in MRS broth for 24 hours then transferred to new culture mediums. Afterward, these mediums were exposed to RF-EMF for 15, 30, 45 and 60 minutes. The control samples were sham-exposed. After 72 hours of incubation on MRS agar cell counts were enumerated.Results: Exposure for 30, 45 and 60 minutes significantly increased the growth of L. acidophilus and L. Casei. No difference was found between the growth of the samples exposed to RF-EMF for 15 minutes compared to that of sham-exposed bacteria. In addition, lactic acid concentration in L. acidophilus medium was amplified after 15, 30 and 45 minutes of exposure. However, in L. Casei samples, only 30 and 60 min exposures could stimulate the production of lactic acid. Conclusion: L. acidophilus and L. Casei probiotic bacteria exposed for a short time to radiofrequency electromagnetic radiation (RF-EMF) generated by a widely used commercial Wi-Fi router show significantly increased proliferation and lactic acid production.

Possible effects of different doses of 2.1 GHz electromagnetic radiation on learning, and hippocampal levels of cholinergic biomarkers in Wistar rats

ABSTRACT The present study evaluated whether short-term exposure to different doses of 2.1 GHz radiofrequency electromagnetic radiation (RF-EMR) has different effects on rats’ behaviour and hippocampal levels of central cholinergic biomarkers. Animals were divided into three equal groups namely; group 1 was sham-exposed group, group 2–3 were exposed to 45 V/m and 65 V/m doses of 2.1 GHz frequency for 1 week respectively. Numerical dosimetry simulations were carried out. Object location and Y-maze were used as behavioural tasks. The protein and mRNA expression levels of AChE, ChAT, and VAChT, in the hippocampus were tested using Western Blotting and Real-Time PCR. The impairment performance of rats subjected to 65 V/m dose of 2.1 GHz RF-EMR in both object location and Y-maze tasks was observed. The hippocampal levels of AChE, ChAT, and VAChT, were significantly lower in rats exposed to 65 V/m dose of 2.1 GHz RF-EMR than others. The stronger effect of “65 V/m” dose on both rat’s hippocampal-dependent behavioural performances and hippocampal levels of cholinergic biomarkers may be due to the stronger effect of “65 V/m” dose where rats’ snouts were located at the nearest distance from the monopole antenna. Furthermore, the simulated SAR values were high for 65 V/m electric-field strengths. For the first time, we report the potential dose-dependent effects of short-term exposure to 2.1 GHz radiation on rat’s behavioural performances as well as hippocampal levels of cholinergic biomarkers. Further studies are needed to understand the mechanisms by which RF-EMR influences the function of the central cholinergic system in the brain.

Association of short-term exposure to ambient PM2.5 with hospital admissions and 30-day readmissions in end-stage renal disease patients: population-based retrospective cohort study

ObjectivesTo examine the effect of short-term exposure to ambient fine particulate matter (PM2.5) on all-cause, cardiovascular and respiratory-related hospital admissions and readmissions among patients receiving outpatient haemodialysis.DesignRetrospective cohort study.SettingInpatient hospitalisation...

Tissue-specific genotoxicity and antioxidant imbalance of titanium dioxide nanoparticles (NPTiO2) and inorganic lead (PbII) in a neotropical fish species

The aquatic environment is the major recipient of wastes containing nanoparticles and other contaminants. Titanium dioxide nanoparticles (NPTiO2) are one of the most produced and used nanoparticle worldwide. This study investigated the toxicity of NPTiO2, as well as the toxicity interaction between NPTiO2 and lead (Pb), in response to genetic and biochemical biomarkers using freshwater fish Rhamdia quelen, as an animal model. The results showed genotoxicity in blood and kidney tissues. No effect of NPTiO2 alone or in co-exposure with Pb on liver genotoxicity were observed. Alterations in the antioxidant hepatic enzymes activities, as well as alterations in glutathione levels indicated that NPTiO2 alone or in co-exposure with Pb can cause antioxidant imbalance. The lipid peroxidation was also raised after exposure to NPTiO2. In general, the results of this study indicated that both NPTiO2 alone and their co-exposure with Pb are capable of producing significant toxic effects in short-term exposure.

Particulate matter (PM10) as a newly identified environmental risk factor for acute gout flares: A time-series study.

This study aimed to investigate the effect of short-term exposure to ambient particulate matter less than 10μm in diameter (PM10) on occurrence of acute gout flares in the general population and identify susceptible groups accordingly. The data of emergency department (ED) cases with acute gout flare in Incheon city, Korea between January 1st 2008 and December 31st 2015 were collected from the National Health Insurance Service claims data. The levels of PM10 and meterological measurements were provided by the Ministry of Environment and the National Meterological Office, respectively. To estimate the risk of daily ED visits due to acute gout flare, these time-series data set were analyzed using generalized additive models with Poisson distribution, including daily average PM10 level, temperature, relative humidity, day of the week, national holiday, season, and date. The risk of daily ED visits for acute gout flares per interquartile range increment of the average daily PM10 levels significantly increased in the cumulative lag 0-7 model (relative risk, 1.018; 95% confidence interval, 1.008-1.027, P<0.001). In particular, men aged≥40 years and those with a history of diabetes mellitus or gout were significantly at a high risk of acute gout flares by subgroup analysis. Our time-series study demonstrated a modest, but significant effect of short-term exposure to PM10 on ED visits for acute gout flares. Ambient PM10 may be a newly identified environmental risk factor for acute gout flares.

Gemini Surfactant as a Template Agent for the Synthesis of More Eco-Friendly Silica Nanocapsules

Silica mesoporous nanocapsules are a class of “smart” engineered nanomaterials (ENMs) applied in several fields. Recent studies have highlighted that they can exert deleterious effects into marine organisms, attributed to the use of the toxic cationic surfactant N-hexadecyl-N,N,N-trimethylammonium bromide (CTAB) during the synthesis of ENMs. The present study reports the successful synthesis and characterization of novel gemini surfactant-based silica nanocapsules. The gemini surfactant 1,4-bis-[N-(1-dodecyl)-N,N-dimethylammoniummethyl]benzene dibromide (QSB2-12) was chosen as a more environmentally-friendly replacement of CTAB. Nanocapsules were characterized by scanning electron microscopy (SEM), Fourier-transformed infrared spectroscopy (FTIR), dynamic light scattering (DLS), thermogravimetric analysis (TGA) and N2 adsorption-desorption isotherms. Short-term exposure effects of new ENMs were evaluated in four marine species (Nannochloropsis gaditana, Tetraselmis chuii and Phaeodactylum tricornutum) and the microcrustacean (Artemia salina). The replacement of the commercial cationic surfactant by the gemini surfactant does not change the structure nor the environmental behaviour in seawater of the newly synthesised silica nanocontainers. Additionally, it is demonstrated that using gemini surfactants can reduce the toxicity of novel silica nanocapsules towards the tested marine species. As a result, environmentally-friendly ENMs can be obtained based on a safe-by-design approach, thereby fitting the concept of Green Chemistry.

Environmental risk analysis of pharmaceuticals on freshwater phytoplankton assemblage: effects on alpha, beta, and taxonomic diversity.

Antibiotics and nonsteroidal anti-inflammatory drugs (NSAIDs) have a wide range of bioactivities and are released into the ecosystem in large amounts. Heretofore, little information is available regarding their potential risk to the phytoplankton assemblage. Different alpha, taxonomic, and beta diversity measures were investigated and linked to the spatial variation of nine drugs. Distance-based redundancy analysis (dbRDA) indicated that pharmaceutical pollution had adverse effects on both phytoplankton diversity and taxonomic structure leading to the existence of congeneric taxa. However, different phytoplankton groups respond differently to different pharmaceuticals and Cyanoprokaryotes was suggested as the most sensitive group. According to the EC50 value and the detected concentration for each drug, a hazard index (Hq) was calculated for each polluted site to investigate environmental risk analysis. Increasing Hq values exhibited negative effects on phytoplankton diversity. Phytoplankton community was characterized by high beta diversity values, which suggested that microalgae were able to disperse and select suitable environmental conditions. High beta diversity values were driven by species difference rather than species replacement due to the disappearance of most sensitive taxa from highly polluted sites. Additionally, microalgae were classified into different morpho-functional groups (FGs), and principal component analysis (PCA) indicated that different FGs had different responses to pharmaceutical pollution. A laboratory toxicity experiment was also conducted to identify the negative effects of short-term exposure to low doses of paracetamol and ciprofloxacin.

Short Term Exposure Effects of Trichostatin a

Histone deacetylase inhibitors (HDACi) are small molecules that increase acetylation of lysine residues by ultimately inhibiting the activity of histone deactylase enzymes. These anticancer agents affect epigenetic and non-epigenetic regulation resulting in cell cycle arrest of cancer cell lines. We have conducted high throughput screen to elucidate effect of short term exposure to Trichostatin A on transcriptional activity of a model cell line. We have tested short term exposure (2h) of Trichostatin A (10uM) on ARPE-19 cell line. In effort to define the molecular signature of TSA we have conducted downstream analyses of transcriptional expression. We have selected genes that showed at least 1.5 fold expression change and were significant (p&amp;lt;0.01). We identified 140 genes that were upregulated and 138 were downregulated. We observe a highly significant enrichment of genes involved dysregulation of p53 pathway (p-value 0.00001). Furthermore we observe a highly significant dysregulation of CREBBP pathway - a protein encoded by this gene has intrinsic histone acetyltransferase activity and also acts as a scaffold to stabilize additional protein interactions with the transcription complex. CREBBP autoacetylation is required for binding to protein substrates, such as acetylated histones and acetylated TP53/p53. KEGG pathways reports aberration of JAK-STAT pathway as well as signaling of Fibroblast Growth Factor Receptor 3 (FGFR3). Using this gene signature further disease perturbation result in a upregulated signature similar to that of Diamond-Blackfan anaemia (p=1.681e-11, OR=8.35). As an independent confirmation we find similar dysregulation patterns as caused by TSA (p=4.8e-86) from other deposited experiments within GEO Omnibus. These preliminary signatures offer a glance into the transcriptional landscape as elicited by short-term exposure to TSA. Further experiments warrant a more comprehensive observations into the mode of action as well as biomarkers that may be useful to design optimal, TSA-based treatments. Disclosures Przychodzen: Vanda Pharmaceuticals: Current Employment, Current equity holder in publicly-traded company. Smieszek:Vanda Pharmaceuticals: Current Employment, Current equity holder in publicly-traded company. Polymeropoulos:Vanda Pharmaceuticals: Current Employment, Current equity holder in publicly-traded company. Polymeropoulos:Vanda Pharmaceuticals: Current Employment, Current equity holder in publicly-traded company.

Short-term effects of exposure to air pollution on biophysical parameters of skin in a panel of healthy adults.

Little research on impact of air pollution on human skin is available. We aimed to clarify the association between acute exposure to criteria air pollutant with biophysical characteristics of the skin. We followed a panel of 20 volunteers free of any skin diseases in skin evaluation study in Tehran, Iran from April 2017 to April 2018. Two distinct body parts including middle forehead and inside the right upper arm were evaluated at six time periods. The associations of the weighted averages of personal exposure to air pollutants at 24 hours up to 6 days, and multiday average before the skin assessment with biophysical characteristics of normal skin including sebum content, hydration, transepidermal water loss (TEWL), erythema index, melanin index, pH, temperature, friction, and elasticity were assessed in a random intercept linear mixed effects modeling approach. We observed significant positive association for the arm sebum content with exposure to PM2.5 , and SO2 ; the arm and forehead TEWL with NO2 , the arm and forehead friction with O3 , and forehead hydration with PM2.5 and PM10 in early lags. We found significant negative association for the arm melanin index, elasticity, and erythema index with exposure to O3 ; and forehead elasticity with PM2.5 and PM10 . Our results provided some evidence that short-term exposure to particulate and gaseous air pollutants have detrimental effects on biophysical and biomechanical properties of skin. The association varied across body area and depended on pollutant type.

Effect of short-term exposure to indoor particulate matter on noctunal blood pressure dipping among hypertensive adults in ibadan: a pilot study

Abstract Introduction While blunted nocturnal blood pressure is a major risk factor for cardiovascular events, limited information exist on the association between indoor particulate and circadian blood pressure variation. We report the association of short-time exposure to PM2.5 and PM10 with blunted nocturnal blood pressure among hypertensive adults in Ibadan. Purpose This study was to determine the association between short time exposure to air particulates and blunted nocturnal blood pressure among people of African descent. Methods We conducted a cross-sectional study among fifty hypertensive cases attending our University College Hospital. A 24-hours blood pressure (BP) monitoring was carried out at the same time as indoor particulate matter monitoring in their homes. Exposure to indoor fine particles (PM2.5) and thoracic particles (PM10) was estimated using a real-time particle counter and compared to the World Health Organization (WHO) 24-hours threshold of 25 μg/m3 and 50 μg/m3 for PM2.5 and PM10 respectively. All monitoring was carried out over a 24-hours period during the wet season. Linear regression model was fitted to determine predictors of non-dipping hypertension. Results Of the 50 hypertensive patients studied, 5 (10.0%), 39 (78.0%), 6 (12.0%) were reverse dippers, non-dippers and dippers respectively. The mean indoor PM2.5 (44.17±19.18 μg/m3) and PM10 (60.10±27.13 μg/m3) among the non-dippers were significantly higher than values obtained among dippers (PM2.5 = 22.97±10.19 μg/m3; PM10 = 29.51±12.74 μg/m3); p&amp;lt;0.0001, and the WHO threshold limit. More non-dippers than dippers (54.5% vs 37.8%) used unimproved fuel such as firewood for cooking. PM10 was an independent predictors of non-dipping status in our regression analysis. Conclusion Short-term exposure to indoor PM air pollution was associated with blunted nocturnal blood pressure. Therefore air pollution reduction strategies through improved cooking pattern is advocated in order to prevent future cardiovascular events. Funding Acknowledgement Type of funding source: None

Pollutant-sex specific differences in respiratory hospitalization and mortality risk attributable to short-term exposure to ambient air pollution

BackgroundMany studies have reported associations of individual pollutants with respiratory hospitalization and mortality based on different populations, which makes it difficult to directly compare adverse health effects among multiple air pollutants. ObjectivesThe study goal is to compare acute respiratory-related hospitalization and mortality associated with short-term exposure to three ambient air pollutants and analyze differences in health risks by season, age and sex. MethodsHourly measurements of air pollutants (ozone, NO2, PM2.5) and temperature were collected from ground-monitors for 24 cities along with daily hospitalization (1996–2012) and mortality (1984–2012) data. National associations between air pollutant and health outcome were estimated for season (warm, cold vs. year-round), age (base ≥ 1, seniors > 65), and sex (females ≥ 1 and males ≥ 1) using Bayesian hierarchical models. ResultsOverall, the three air pollutants were significantly associated with acute respiratory health outcomes at different lag-days. For respiratory hospitalization, the increased risks in percent changes with 95% posterior intervals for a 10-unit increase in each pollutant were: ozone (lag1, 0.7% (0.4, 0.9)), NO2 (lag0, 0.7% (0.1, 1.4)), and PM2.5 (lag1, 1.3% (0.7, 1.9)). For respiratory mortality: ozone (lag2, 1.2% (0.4, 1.9)), NO2 (lag1, 2.1% (0.6, 3.5)), and PM2.5 (lag1, 0.6% (−1.0, 2.2)). While some differences in risk were observed by season and age group, sex-specific differences were more pronounced. Compared with males, females had a higher respiratory mortality risk (1.8% (0.6, 2.9) vs 0.5% (−0.3, 1.3)) from ozone, a higher respiratory hospitalization risk (0.9% (0.0, 1.8) vs 0.6% (−0.3, 1.4)) but lower mortality risk (1.4% (−1.0, 3.7) vs 2.2% (0.4, 4.0)) from NO2, and a lower hospitalization risk (0.7% (−0.2, 1.7) vs 1.8% (1.0, 2.6)) from PM2.5. ConclusionThis study reports significant health effects of short-term exposure to three ambient air pollutants on respiratory hospitalization (ozone≈NO2 < PM2.5 per-10 unit; ozone>NO2 ≈ PM2.5 per-IQR) and mortality (ozone≈NO2 > PM2.5) in Canada. Pollutant-sex-specific differences were found, but inconclusive due to limited biological and physiological explanations. Further studies are warranted to understand the pollutant-sex specific differences.