The coastal reclamation, as one of the most extreme transformations of the ocean space by humans, still lacks scientific quantitative evaluating methods to a large extent, compared with the evolution of land use patterns. A cumulative ecological impacts of reclamation (RCEI) was established in our study based on ecological influence characteristics of different reclamation types, and the attenuation effect of reclamation on adjacent areas. It was characterized by spatio-temporal features in decades. Here, we estimated that the cumulative reclamation area in the Bohai Sea from 1985 to 2018 was 5839.5 km2. Under the influence of human activity, proportions of the industrial and urban boundary, marine construction boundaries (e.g., ports, wharves, and bridges), and protective dams were increased significantly, which led to a sharp increase of the RCEI. In addition, spatio-temporal changes of reclamation were affected by the combination of population growth, economic development, urbanization, industrialization, and marine industry development in coastal cities. These results provided an important historical reference for tracking future development of the Bohai Sea by humans and provided basic data support for the development and protection of the ocean.