Effect Of Pretreatment Research Articles

Effect and mechanism of iron-carbon micro-electrolysis pretreatment of organic peroxide production wastewater.

The wastewater from organic peroxide production has high chemical oxygen demand (COD) concentration and poor biodegradability, so it is necessary to find a cost-effective treatment method. The iron-carbon microelectrolysis (IC-ME) technology was used to pretreat the organic peroxide production wastewater, and the influence of reaction conditions on the removal effect of pollutants and the degradation mechanism were studied. The effects of initial pH, iron filings, iron-carbon ratio, and reaction time on the wastewater treatment were investigated by single-factor and response surface optimization experiments, and the degradation mechanism was analyzed by three-dimensional fluorescence spectroscopy, UV-Vis, and gas chromatography mass spectrometry (GC-MS). The experimental results showed that the COD removal efficiency was 35.67% and the biodegradability of wastewater was increased from 0.113 to 0.173 under the conditions of initial pH of 3.1, the dosage of iron filings of 30.5g/L, the ratio of iron-carbon of 1.01, and the reaction time of 122.8min, and the process of IC-ME for degrading COD of wastewater from the production of organic peroxide was consistent with the secondary reaction. The IC-ME process could decompose macromolecular organic compounds such as tyrosine proteins and aromatic proteins, and improve the biodegradability of wastewater. It provides a theoretical reference for the practical application of IC-ME to treat this type of wastewater.

Effects of Post-Harvest Processing Methods on the Quality of Radix et Rhizoma Salviae Miltiorrhizae

The effect of pretreatment, drying and storage methods on maintaining the quality of Radix et Rhizoma Salviae mitiorrhzae postharvest was studied. The experiments used organic acids as acid citric and sodium bisulfate solution to evaluate the pretreatment of Radix et Rhizoma Salviae mitiorrhzae. Rhizome and roots of Salvia miltiorrhiza Bunge were pretreated and dried by convection drier and heat pump drier and were preserved by low pressure. The results showed that dipping acid citric solution pH 2.5-3 for 30 minutes restricted the developing of total microorganisms. Using heat pump drier were good sensory quality and retaining tanshinone IIA, salvianolic acid content in Radix et Rhizoma Salviae mitiorrhzae. The method of preserving Radix et Rhizoma Salviae mitiorrhzae by the low pressure 200 mmHg, and 400 mmHg after 9 months at ambient temperature (25-30 oC), humidity (80-85%) maintained better quality than the control. Beside, the moisture’s medicine, tanshinone IIA, salvianolic acid content, and total microorganisms in the experiment samples changed slowly during the storage period.

Microstructure and physicochemical properties: effects of different pretreatment combined with dual‐frequency ultrasound on quality of large yellow croaker (Pseudosciaena crocea) during cold storage

SummaryThe quality changes of cold‐stored (4 °C) large yellow croaker (Pseudosciaena crocea) were studied by using stable chlorine dioxide combined with dual‐frequency ultrasound assisted‐slightly acidic electrolysed water (CUSS). The results of microbial indicators demonstrated that CUSS treatment inhibited microbial growth. Moreover, CUSS treatment had lower values of pH, total volatile basic nitrogen (TVB‐N) and thiobarbituric acid (TBA), and higher immobilised water content. CUSS treatment also inhibited the degradation of inosine monophosphate, suppressed the accumulation of hypoxanthine riboside and hypoxanthine. Through the results of texture profile analysis (TPA), protein degradation, and scanning electron microscopy (SEM), CUSS treatment could effectively protect the protein structure and maintain good texture characteristics of large yellow croaker. According to microbial indexes, K‐value, and sensory analysis, compared with dual‐frequency ultrasound treatment, CUSS treatment could prolong the shelf‐life of large yellow croaker for another six days. Therefore, CUSS treatment is a potential solution to preserve large yellow croaker, improving its protein characteristics.

Dimensions, microstructure, chemical, and hygroscopic analysis of steam-exploded bamboo fibers at different ages

Bamboo fibers (BFs) at different ages (1, 3, and 5 years old) were prepared by pretreatment and steam explosion. The results demonstrated that the separation degrees of BFs increased with the age of the bamboo. The combined effects of pretreatment and steam explosion partially removed the hemicellulose and lignin from BFs, while the crystalline structure of cellulose remained unaltered. The water vapor adsorption isotherms of BFs at different ages all showed a typical S-shape and could be classified as type II, and the hysteresis of BFs increased with the age of the bamboo. After pretreatment and steam explosion, the tissues in the vascular bundle were effectively crushed, and the parenchyma tissues were dislodged and separated from the fiber sheath. During the steam explosion process, hot steam penetrated the bamboo’s pores, including the pores induced by pretreatment. This caused fractures and delamination in the interface between the vascular bundle and the parenchyma tissues. These cracks progressed layer by layer along the vascular bundle interface, leading to the fracture of the bamboo.

Effect of UV pretreatment on the source control of floR during subsequent biotreatment of florfenicol wastewater.

UV photolysis has been recommended as an alternative pretreatment method for the elimination of antibacterial activity of antibiotics against the indicator strain, but the pretreated antibiotic intermediates might not lose their potential to induce antibiotic resistance genes (ARGs) proliferation during subsequent biotreatment processes. The presence of florfenicol (FLO) in wastewater seriously inhibits the metabolic performance of anaerobic sludge microorganisms, especially the positive correlation between UV irradiation doses and ATP content, while it did not significantly affect the organics utilization ability and protein biosynthetic process of aerobic microorganisms. After sufficient UV pretreatment, the relative abundances of floR from genomic or plasmid DNA in subsequent aerobic and anaerobic biotreatment processes both decreased by two orders of magnitude, maintained at the level of the groups without FLO selective pressure. Meanwhile, the abundances of floR under anaerobic condition were always lower than that under aerobic condition, suggesting that anaerobic biotreatment systems might be more suitable for the effective control of target ARGs. The higher abundance of floR in plasmid DNA than in genome also indicated that the potential transmission risk of mobile ARGs should not be ignored. In addition, the relative abundance of intI1 was positively correlated with floR in its corresponding genomic or plasmid DNA (p < 0.05), which also increased the potential horizontal transfer risk of target ARGs. This study provides new insights into the effect of preferential UV photolysis as a pretreatment method for the enhancement of metabolic performance and source control of target ARGs in subsequent biotreatment processes. KEY POINTS: • Sufficient UV photolytic pretreatment efficiently controlled the abundance of floR • A synchronous decrease in abundance of intI1 reduced the risk of horizontal transfer • An appreciable abundance of floR in plasmid DNA was a potential source of total ARGs.

Effect of Plasma pretreatment and Graphene oxide ratios on the transport properties of PVA/PVP membranes for fuel cells

In this study, a novel proton-conducting polymer electrolyte membrane based on a mixture of polyvinyl alcohol (PVA)/polyvinyl pyrrolidone (PVP) (1:1) mixed with different ratios of graphene oxide (GO) and plasma-treated was successfully synthesized. Dielectric barrier dielectric (DBD) plasma was used to treat the prepared samples at various dose rates (2, 4, 6, 7, 8, and 9 min) and at fixed power input (2 kV, 50 kHz). The treated samples (PVA/PVP:GO wt%) were soaked in a solution of styrene and tetrahydrofuran (70:30 wt%) with 5 × 10−3 g of benzoyl peroxide as an initiator in an oven at 60 °C for 12 h and then sulfonated to create protonic membranes (PVA/PVP-g-PSSA:GO). The impacts of graphene oxide (GO) on the physical, chemical, and electrochemical properties of plasma-treated PVA/PVP-g-PSSA:x wt% GO membranes (x = 0, 0.1, 0.2, and 0.3) were investigated using different techniques. SEM results showed a better dispersion of nanocomposite-prepared membranes; whereas the AFM results showed an increase in total roughness with increasing the content of GO. FTIR spectra provide more information about the structural variation arising from the grafting and sulfonation processes to confirm their occurrence. The X-ray diffraction pattern showed that the PVA/PVP-g-PSSA:x wt% GO composite is semi-crystalline. As the level of GO mixing rises, the crystallinity of the mixes decreases. According to the TGA curve, the PVA/PVP-g-PSSA:x wt% GO membranes are chemically stable up to 180 °C which is suitable for proton exchange membrane fuel cells. Water uptake (WU) was also measured and found to decrease from 87.6 to 63.3% at equilibrium with increasing GO content. Ion exchange capacity (IEC) was calculated, and the maximum IEC value was 1.91 meq/g for the PVA/PVP-g-PSSA: 0.3 wt% GO composite membrane. At room temperature, the maximum proton conductivity was 98.9 mS/cm for PVA/PVP-g-PSSA: 0.3 wt% GO membrane. In addition, the same sample recorded a methanol permeability of 1.03 × 10−7 cm2/s, which is much less than that of Nafion NR-212 (1.63 × 10−6 cm2/s). These results imply potential applications for modified polyelectrolytic membranes in fuel cell technology.

Photosynthetic mechanisms underlying NaCl-induced salinity tolerance in rice (Oryza sativa)

BackgroundSalinity stress is an environmental constraint that normally develops concurrently under field conditions, resulting in drastic limitation of rice plant growth and grain productivity. The objective of this study was to explore the alleviating effects of NaCl pre-treatment on rice seedlings as well as the salt tolerance mechanisms by evaluating morph-physiological traits.ResultsVariety Huanghuazhan, either soaked in distilled water or 25 mg/L Prohexadione calcium (Pro-Ca), were first hardened with varying concentrations of NaCl solutions (0 and 50 mM NaCl), and then subjected to varying degrees of salt stress (0 and 100 mM NaCl), indicated by S0, S1, S2 and S3, respectively. Growth analysis suggested that NaCl-pretreatment improved the root/shoot ratio in water-soaked rice plant at DAP 0. Data related to the reaction center density, photosynthetic electron transport efficiency, trapping efficiency were compared before (CK) using performance Index (PIabs). Compared to S2 (Pro-Ca-S2) treatment, PIabs did not show any difference with plants pre-treated with NaCl (S3 or Pro-Ca-S3). Rather than PIabs, significant difference was found in photosynthetic electron transport efficiency (ΨEo). The ΨEo value in Pro-S2 was significantly lowered as compared to Pro-S3 treatment at DAP 7, and the decrease rate was about 6.5%. Correlation analysis indicated leaf PIabs was weak correlated with plant biomass while the quantum yield for reduction of the PSI end electron acceptors, trapped energy flux per reaction center and PSII antenna size displayed strong positive correlation with biomass. Additional analysis revealed that 100 mM NaCl significantly reduced leaf linear electron flux under low-light conditions, regardless of whether seedlings had been pre-treated with 50 mM NaCl or not.ConclusionsNaCl-induced salt tolerance was related to the robust photosynthetic machinery.

Comparative analysis of photo-fermentation hydrogen production from pretreated corn stover by deep eutectic solvents

To compare the pretreatment effect of deep eutectic solvents (DESs) on corn stover, three types of DESs (acid, neutral, and alkaline) were used to pretreat corn stover, and their effects on lignin removal were investigated. Then the performance of photo-fermentation hydrogen production (PFHP) using DESs pretreated corn stover was compared and analyzed. The results showed that acid DESs had the best lignin removal efficiency, and the lignin removal percentages of choline chloride/formic acid (ChCl/Fac) and choline chloride/oxalate (ChCl/Oac) were 64.7% and 65.6%, respectively. The alkaline DESs had the second highest removal of lignin, while the neutral DESs had a poor effect on lignin removal. The comparison of hydrogen production indicated that the corn stover pretreated with ChCl/Oac had the best hydrogen production effect. In conclusion, the DESs pretreatment can obviously remove the lignin in corn stover, and then improve the hydrogen production capacity and efficiency of photo-fermentation, which provides a potential pretreatment method for the efficient utilization of straw biomass.

Effect of ultrasound-ethanol immersion, microwave and starch-blanching pretreatments on drying kinetics, rehydration, and quality properties of beetroot chips

Abstract For the first time, the impact of ultrasound-ethanol immersion as a non-thermal pretreatment (NTPT) and coating-blanching in starch solutions as a thermal pretreatment (TP) on the convective drying of beetroot was evaluated. The beetroot was exposed to ethanol immersion (E), ultrasound (U), and ultrasound-ethanol immersion (UE). Besides, TP pretreatment was performed by blanching the beetroot at steam (SB), water (WB), starch-coating solutions, and microwave (M). The hot air drying was conducted at 90 °C and air velocity of 1.2 m/s. The maximum decreases in the drying time were observed at UE30 (64.29 %) and the sample blanched at native corn starch solution (60.17 %). Moisture diffusion coefficients ranged from 0.851 to 2.312 × 10−9 m2/s. The friction drag force, convective heat, and mass transfer coefficients were 2.840 × 10−6 N, 59.368 W/m2 K, and 0.0492 m/s, respectively. The thermal conductivity, specific heat, and density ranged from 0.464 to 0.615 W/m. K, 3164–4071 J/kg. K, and 798.9 to 1055.9 kg/m3, respectively. The maximum values of rehydration ratio at non-thermal (NTPT) and thermal pretreatments (TP) were observed at U30 and the sample blanched at the modified starch solution (MCS), respectively. The total phenolic contents of the NTP sample decreased while those for the TP samples increased due to boosted polyphenol synthesis at high temperatures. Both U and E samples caused a decrease in the total antioxidant activity, while they increased the anthocyanin content of beetroot samples. Pretreatments reduced the hardness owing to changes in the microstructure of the sample. U, E, and M pretreatments increased the brightness of samples, and the minimum color change compared with control samples was observed by UE30 pretreatment. The UE and blanching at a starch solution could be selected for improving the drying characteristics of beetroots at an industrial scale.

Nicotinamide mononucleotide alleviates seizures via modulating SIRT1-PGC-1α mediated mitochondrial fusion and fission.

Both human and animal experiments have demonstrated that energy metabolism dysfunction in neurons after seizures is associated with an imbalance in mitochondrial fusion/fission dynamics. Effective neuronal mitochondrial dynamics regulation strategies remain elusive. Nicotinamide mononucleotide (NMN) can ameliorate mitochondrial functional and oxidative stress in age-related diseases. But whether NMN improves mitochondrial energy metabolism to exert anti-epileptic effects is unclear. This study aims to clarify if NMN can protect neurons from pentylenetetrazole (PTZ) or Mg2+ -free-induced mitochondrial disorder and apoptosis via animal and cell models. We established a continuous 30-day PTZ (37 mg/kg) intraperitoneal injection-induced epileptic mouse model and a cell model induced by Mg2+ -free solution incubation to explore the neuroprotective effects of NMN. We found that NMN treatment significantly reduced the seizure intensity of PTZ-induced epileptic mice, improved their learning and memory ability, and enhanced their motor activity and exploration desire. At the same time, invitro and invivo experiments showed that NMN can inhibit neuronal apoptosis and improve the mitochondrial energy metabolism function of neurons. In addition, NMN down-regulated the expression of mitochondrial fission proteins (Drp1 and Fis1) and promoted the expression of mitochondrial fusion proteins (Mfn1 and Mfn2) by activating the SIRT1-PGC-1α pathway, thereby inhibiting PTZ or Mg2+ -free extracellular solution-induced mitochondrial dysfunction, cell apoptosis, and oxidative stress. However, combined intervention of SIRT1 inhibitor, Selisistat, and PGC-1α inhibitor, SR-18292, eliminated the regulatory effect of NMN pre-treatment on mitochondrial fusion and fission proteins and apoptosis-related proteins. Therefore, NMN intervention may be a new potential treatment for cognitive impairment and behavioral disorders induced by epilepsy, and targeting the SIRT1-PGC-1α pathway may be a promising therapeutic strategy for seizures.

Structural characterization of lignin from the green pretreatments for co-producing xylo-oligosaccharides and glucose: Toward full biomass utilization

Three green non-enzymatic catalysis pretreatments (NECPs) including autohydrolysis, subcritical CO2-assisted seawater autohydrolysis, and inorganic salt catalysis were utilized to simultaneously produce xylo-oligosaccharides (XOS), glucose, and cellulolytic enzyme lignin (CEL) from sugarcane bagasse (SCB). The yield of XOS in all three NECPs was over 50 % with a competitive glucose yield of enzymatic hydrolysis. And the effects of different pretreatments on the chemical structure and composition of CEL samples were also investigated. The pretreatments significantly increased the thermal stability, yield, and purity of the CEL samples. Moreover, the net yield of lignin was 58.3 % with lignin purity was 98.9 % in the autohydrolysis system. Furthermore, there was a decrease in the molecular weight of CEL samples as the pretreatment intensity increased. And the original lignin structural units sustained less damage during the NECPs, due to the cleavage of the β-O-4 bonds dominating lignin degradation. Meanwhile, these pretreatments increased the phenolic-OH in CEL samples, making the lignin more reactive, and enhancing its subsequent modification and utilization. Collectively, the described techniques have demonstrated practical significance for the coproduction of XOS and glucose, and lignin, providing a promising strategy for full utilization of biomass.

24-Epibrassinolide Reduces Drought-Induced Oxidative Stress by Modulating the Antioxidant System and Respiration in Wheat Seedlings.

Brassinosteroids (BRs) represent a group of plant signaling molecules with a steroidal skeleton that play an essential role in plant adaptation to different environmental stresses, including drought. In this work, the effect of pretreatment with 0.4 µM 24-epibrassinolide (EBR) on the oxidant/antioxidant system in 4-day-old wheat seedlings (Triticum aestivum L.) was studied under moderate drought stress simulated by 12% polyethylene glycol 6000 (PEG). It was revealed that EBR-pretreatment had a protective effect on wheat plants as evidenced by the maintenance of their growth rate, as well as the reduction in lipid peroxidation and electrolyte leakage from plant tissues under drought conditions. This effect was likely due to the ability of EBR to reduce the stress-induced accumulation of reactive oxygen species (ROS) and modulate the activity of antioxidant enzymes. Meanwhile, EBR pretreatment enhanced proline accumulation and increased the barrier properties of the cell walls in seedlings by accelerating the lignin deposition. Moreover, the ability of EBR to prevent a drought-caused increase in the intensity of the total dark respiration and the capacity of alternative respiration contributes significantly to the antistress action of this hormone.

Establishment of Detection Model of Soybean Quality Traits by Near Infrared Spectroscopy

Background: Rapid prediction with near infrared (NIR) spectroscopy on quality traits is pretty popular recently, for the convenience and simple operation. But to make good use of this technology, precise and suitable calibration equations are very important to get dependable result. In this study, we mostly refer to the building of the equation and how the pretreatment effect them. Methods: In this paper, near infrared (NIR) spectroscopy was used to simultaneously predict the quality traits of soybean, including oil content, protein content, oleic acid content, linoleic acid content, stearic acid content. Near infrared spectral data of a total of 112 samples is collected from given materials in Chongqing. Samples were scanned from 1000 nm to 2500 nm using a monochromator instrument (SuperNIR-2700). Calibration equations were developed from NIR data using partial least squares (PLS) regression with internal cross validation. In addition, in this study, we also cover the affection of different pre-treatments to the different calibration equations predicting different quality traits. And measure the effect with three indicators including R, SECV and RPD. Result: Eventually we find the most suitable combination of pre-treatments for each calibration equation predicting a certain trait soybean. The present study would lay the foundations of rapid detection of quality traits in soybean.

The influence of pretreatments on the quality characteristics and in vitro biological activity of freeze-dried Thai Tom-Yum ingredients

This study aimed to investigate the effects of pretreatments on quality characteristics, bioaccessibility of phenolic compounds, and antioxidant activities (AA) of freeze-dried Thai Tom-Yum ingredients during in vitro digestion. Hot water (B), steam (S), microwave blanching (MW), ultrasound (US), and blanching with dipping in chemical solution (D) were performed on lemongrass, red onion, kaffir lime leaves, chili, galangal, and coriander. In most samples, D significantly lowered water activity (Aw) and moisture content (MC) while preserving color attributes. D was the best pretreatment with the highest total phenolic compounds (TPC) for lemongrass, red onion, and galangal, US for kaffir lime leaves and chili, and S for coriander. The AA assessed by DPPH and FRAP showed a correlation with TPC values (0.731 and 0.576 at p < 0.01). Bioaccessibility of phenolic compounds and FRAP in selected herbs were higher under intestinal phase. After digestion, kaffir lime leaves treated with US had the highest TPC and FRAP values, ranging from 26.63 to 34.34 mg GAE/g db and 80.07–105.07 μmol FeSO4/g db, respectively. A decrease in DPPH values was observed in lemongrass, red onion, chili, and galangal. These findings show that pretreatments and freeze-drying have considerable effects on the qualitative attributes, bioaccessibility of phenolic compounds, and AA of dried herbs.

Drying of Capsicum annum (Kashmiri Long 1): Effect of pre-treatments and drying temperatures on quality parameters, colour and drying kinetics

Drying of Capsicum annum (Kashmiri Long 1): Effect of pre-treatments and drying temperatures on quality parameters, colour and drying kinetics

Effects of Pretreatment on Stability of Peanut Oil Bodies and Functional Characteristics of Proteins Extracted by Aqueous Enzymatic Method.

Effects of dry and wet grind on peanut oil and protein yield, oil bodies (OBs) stability, fatty acid composition, protein composition and functional characteristics were systematically analyzed. Results showed that peanut oil and protein yields reached highest at dry grind 90 s (92.56% and 83.05%, respectively), while peanut oil and protein yields were 94.58% and 85.36%, respectively, at wet grind 120 s. Peanut oil and protein yields by wet grind was 2.18% and 2.78% higher than that of dry grind, respectively. Surface protein concentration (Г) and absolute value of zeta potential of OBs extracted by wet grind (WOBs) were 11.53 mg/m 2 and 18.51 mV, respectively, which were higher than OBs extracted by dry grind (DOBs), indicating stability of WOBs was higher than DOBs. Relative contents of oleic acid and linoleic acid in peanut oil, essential and hydrophobic amino acids in protein extracted by wet grind were higher than dry grind. There was little difference in protein composition between wet and dry grind, but thermal denaturation degree of protein obtained by wet grind was lower than dry grind. Solubility, oil retention, emulsion stability, foaming and foam stability of protein obtained by wet grind were better than dry grind. Results from this study provided theoretical basis for grind pretreatment selection of aqueous enzymatic method.

Effect of universal adhesive pretreatments on the bond strength durability of conventional and adhesive resin cements to zirconia ceramic.

This study aimed to evaluate the effect of pretreatment of three different universal adhesives (Single Bond Universal [SBU], All-Bond Universal [ABU], and Prime&Bond universal [PBU]) on the bonding durability of an adhesive (Panavia F 2.0, PF) and a conventional (Duo-Link, DL) resin cements to air-abraded zirconia. Rectangular-shaped zirconia specimens were prepared. The chemical composition and surface energy parameters of the materials were studied by Fourier transform infrared spectroscopy and contact angle measurement, respectively. To evaluate resin bonding to the zirconia, all the bonding specimens were immersed in water for 24 h and the specimens to be aged were additionally thermocycled 10000 times before the shear bond strength (SBS) test. The materials showed different surface energy parameters, including the degree of hydrophilicity/hydrophobicity. While the DL/CON (no pretreatment) showed the lowest SBS and a significant decrease in the value after thermocycling (P < .001), the PF/CON obtained a higher SBS value than the DL/CON (P < .001) and no decrease even after thermocycling (P = .839). When the universal adhesives were used with DL, their SBS values were higher than the CON (P < .05), but the trend was adhesive-specific. In conjunction with PF, the PF/SBU produced the highest SBS followed by the PF/ABU (P = .002), showing no significant decrease after thermocycling (P > .05). The initial SBS of the PF/PBU was similar to the PF/CON (P = .999), but the value decreased after thermocycling (P < .001). The universal adhesive pretreatment did not necessarily show a synergistic effect on the bonding performance of an adhesive resin cement, whereas the pretreatment was beneficial to bond strength and durability of a conventional resin cement.

The NO reduction by CO over NiOx/CeO2 catalysts with a fixed Ni surface density: pretreatment effects on the catalyst structure and catalytic activity

NiOx/CeO2 catalysts were synthesized under various pretreatment conditions. Different pretreatment conditions significantly influenced the activity of the NO reduction by CO reaction.

Effects of preconditioning with heat stress on acute exercise-induced intracellular signaling in male rat gastrocnemius muscle.

Heat stress (HS) induces Akt/mTOR phosphorylation and FoxO3a signaling; however, whether a prior increase in heat shock protein 72 (HSP72) expression affects intracellular signaling following eccentric exercise remains unclear. We analyzed the effects of HS pretreatment on intramuscular signaling in response to acute exercise in 10-week-old male Wistar rats (n = 24). One leg of each rat was exposed to HS and the other served as an internal control (CT). Post-HS, rats were either rested or subjected to downhill treadmill running. Intramuscular signaling responses in the red and white regions of the gastrocnemius muscle were analyzed before, immediately after, or 1 h after exercise (n = 8/group). HS significantly increased HSP72 levels in both deep red and superficial white regions. Although HS did not affect exercise-induced mTOR signaling (S6K1/ERK) responses in the red region, mTOR phosphorylation in the white region was significantly higher in CT legs than in HS legs after exercise. Thr308 phosphorylation of Akt showed region-specific alteration with a decrease in the red region and an increase in the white region immediately after downhill running. Overall, a prior increase in HSP72 expression elicits fiber type-specific changes in exercise-induced Akt and mTOR phosphorylation in rat gastrocnemius muscle.

Effects of magnetic field pretreatment and chloride salt stress on physio-biochemical changes and γ-aminobutyric acid accumulation in germinated brown rice

Effects of magnetic field pretreatment and chloride salt stress on physio-biochemical changes and γ-aminobutyric acid accumulation in germinated brown rice