AbstractTurfgrass roots are essential for growth and development by taking up water and nutrients from the soil. Therefore, improving root characteristics such as root length, root volume, root density, root diameter, and root dry biomass is crucial for improving turfgrass growth and quality. Greenhouse experiments were conducted in 2023 to document the effects of nitrogen (N), phosphorus (P), and potassium (K) fertilizers on the root morphological responses of Penncross creeping bentgrass (Agrostis stolonifera) during establishment. Turfgrasses were grown from seeds in sand‐filled acrylic tubes for around three months. Fertilizer treatments included N, P, K, PK, NK, NP, all three macronutrients (NPK), and a non‐fertilized control (non‐NPK). NP treatment resulted in a greater total root length, root surface area, root volume, and root dry biomass of creeping bentgrass than the other treatments except for NPK treatment. While it is critical to provide sufficient N, P, and K for the optimal development of creeping bentgrass roots during the establishment period, reducing K fertilization had the least impact on the root morphological response of creeping bentgrass. NPK application during turfgrass establishment is context‐dependent, particularly with regard to nutrient availability in the soil. Based on this study, applying a balanced starter fertilizer with adjustments per the soil test results would ensure proper root growth.
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