Effects Of Polyvinyl Chloride Microplastics Research Articles

Wheat (Triticum aestivum L.) seedlings performance mainly affected by soil nitrate nitrogen under the stress of polyvinyl chloride microplastics

Microplastics are exotic pollutants and are increasingly detected in soil, but it remains poorly understood how microplastics impact soil and plant systematically. The present study was conducted to evaluate the effects of polyvinyl chloride microplastics (PVC-MPs) on wheat seedlings performance and soil properties. Under the stress of PVC-MPs, no new substance and functional groups were generated in soil by X-ray diffraction and the fourier transform infrared spectroscopy analyses, whereas the diffraction and characteristic peaks and of soil was affected by PVC-MPs. Wheat seedlings shoot biomass and soil nitrate nitrogen were significantly inhibited by PVC-MPs. Chlorophylls were not significant affected by PVC-MPs. Superoxide dismutase, catalase, and peroxidase activities in wheat seedlings increased, while malondialdehyde and proline contents decreased significantly. Redundancy analysis displayed that wheat seedlings traits can be largely explained by soil nitrate nitrogen. Our results indicate that PVC-MPs have more significant influence on soil structure than on soil substance composition. Moreover, even though antioxidant enzyme activities were improved to respond the stress of PVC-MPs, wheat seedlings are not severely impacted by PVC-MPs. Besides, soil nitrate nitrogen is the main factor on wheat seedlings performance and wheat seedlings are prone to ensure the root growth under the stress of PVC-MPs.

Toxicity of photoaged polyvinyl chloride microplastics to wheat seedling roots

Photoaging-prone and additive-rich polyvinyl chloride microplastics (PVC-MPs) are abundant in the terrestrial environment, However, current knowledge about the effects of PVC-MPs on terrestrial plants is lacking. Herein, we investigated the physicochemical toxicity mechanisms of photoaged PVC-MP components, i.e. leachate (L), leached PVC-particles (P), and unleached PVC-MPs (UAMP), to wheat seedling roots. 108-h photoaged components were more detrimental to root growth than unaged ones, with root length decline by 3.56%− 7.45%, indicating enhanced ecotoxicity. Notably, 108-h aged UAMP displayed more pronounced inhibition to root architecture, nutrient content and root activity, and more significant stimulation on antioxidant systems compared to 108-h aged L and P. The abovementioned phenomena suggested the presence of a synergistic effect between physical damage from P and chemical harm from L. Surface adsorption experiments demonstrated that the adsorption of photoaging induced smaller particles caused physical damage to root system. Exposure treatment suggested that there was appreciable environmental risk posed by photoaged PVC-MP-derived additives, e.g., Irgafos 168-ox and Irganox 1076. Based on principal component analysis (PCA), additives from leachate played a greater role in UAMP ecotoxicity. Therefore, PVC-MP-derived additives require more consideration and put forward an important new aspect for the impact assessment of PVC-MPs in the environment.

Effects of polyvinylchloride microplastics on the toxicity of nanoparticles and antibiotics to aerobic granular sludge: Nitrogen removal, microbial community and resistance genes

Copper oxide nanoparticles (CuO NPs) and ciprofloxacin (CIP) have ecological risk to humans and ecosystems. Polyvinylchloride microplastics (PVC MPs), as a representative of microplastics, may often coexist with CuO NPs and CIP in wastewater treatment systems due to their widespread application. However, the co-impact of PVC MPs in wastewater systems contained with CuO NPs and CIP on nitrogen removal and ecological risk is not clear. In this work, PVC MPs co-impacts on the toxicity of CuO NPs and CIP to aerobic granular sludge (AGS) systems and potential mechanisms were investigated. 10 mg/L PVC MPs co-addition did not significantly affect the nitrogen removal, but it definitely changed the microbial community structure and enhanced the propagation and horizontal transfer of antibiotics resistance genes (ARGs). 100 mg/L PVC MPs co-addition resulted in a raise of CuO NP toxicity to the AGS system, but reduced the co-toxicity of CuO NPs and CIP and ARGs expression. The co-impacts with different PVC MPs concentration influenced Cu2+ concentrations, cell membrane integrity, extracellular polymeric substances (EPS) contents and microbial communities in AGS systems, and lead to a change of nitrogen removal.

Influence of ultraviolet-aging and adsorbed pollutants on toxicological effects of polyvinyl chloride microplastics to zebrafish

Microplastics (MPs) undergo various aging processes and interact with diverse pollutants in the environment. In the present study, we investigated the influence of ultraviolet (UV) aging on the adsorption of organic pollutants by polyvinyl chloride microplastics (mPVC) and explored toxicity variations among pristine, aged, and pollutant-loaded mPVCs to zebrafish. Irradiation of UV for 30 d significantly changed the physiochemical properties of mPVC, leading to more oxygen-containing groups and free radicals (1O2, ·O2−, and ·OH) on mPVC surfaces. The aging process reduced the adsorption of mPVC against a hydrophobic compound chlorpyrifos (CPF) but enhanced the adsorption against a moderately hydrophilic compound erythromycin (ERY). Ingestion of CPF- and ERY-loaded mPVCs resulted in bioaccumulation of the two compounds in zebrafish, suggesting a carrier effect of mPVCs. In toxicity tests, the aged mPVC caused severer gut damages, stronger oxidative stresses, and greater interference with the gut microbiota in zebrafish than the pristine mPVC. The CPF and ERY-loaded mPVCs produced lower oxidative stresses in zebrafish than mPVCs alone, due to fewer radicals on mPVC surfaces after the adsorption of organic contaminants. Notably, the CPF and ERY-loaded mPVCs presented greater effects on fish swimming behaviors and gut microbial compositions, which was associated with the released CPF and ERY from mPVCs within the zebrafish. Overall, the present study demonstrated significant influences of UV-aging and the adsorbed pollutants on the toxicological effects of MPs and highlighted the necessity to perform toxicity studies of MPs using more environmentally relevant MPs.

Hydrochar alleviated the inhibitory effects of polyvinyl chloride microplastics and nanoplastics on anaerobic granular sludge for wastewater treatment

The exposure to microplastics (MPs) and nanoplastics (NPs) has been confirmed to exhibit significant inhibitory effects on anaerobic granular sludge (AGS) for wastewater treatment, with effective mitigation strategies being accordingly imperative. Herein, this study innovatively proposed a strategy for mitigating inhibitory effects of MPs/NPs on AGS system based on coconut shell-derived hydrochar through efficiently capturing the existing polyvinyl chloride microplastics and nanoplastics (PVC-MPs and PVC-NPs) from AGS. The hydrochar increased methane production of AGS from 69.4% to 76.2% and from 65.6% to 91.4% of control when the AGSs were exposed to PVC-MPs and PVC-NPs, respectively. More extracellular polymeric substance (EPS) was secreted with the existence of hydrochar, which enhanced the protective capabilities AGS held to against the negative effects from the external toxicity of PVC-MPs and PVC-NPs, thus maintaining better AGS integrity regarding granule size and cell viability (especially for the PVC-NPs affected AGS). The hydrochar showed stronger adsorption capability to PVC-MPs and PVC-NPs than AGS, confirmed by their characteristics and adsorption kinetic tests. As a result, less plastic particles would attach AGS, inducing less oxidative stress to the microbes. Specially, it would also be less likely for PVC-NPs to penetrate through AGS surface and enter the internal core, retaining better richness of bacteria such as Bacteroidales and Syntrophobacterales in AGS. This work demonstrated hydrochar effectively alleviated the suppression on AGS caused by PVC-MPs and PVC-NPs, providing a novel strategy for improving the wastewater treatment performance under the stress of MPs and NPs.

The combined toxic effects of polyvinyl chloride microplastics and di(2-ethylhexyl) phthalate on the juvenile zebrafish (Danio rerio)

Microplastics (MPs) have the characteristics of large specific surface area, high hydrophobicity and surface charge, so they are easy to combine with other pollutants and cause toxic effects on aquatic organisms. Here, we prepared a polyvinyl chloride-microplastics (PVC-MPs) fragmentation model to simulate the real microplastic state, and characterized its composition, morphology, particle size and zeta potential. On this basis, we used single and compound exposure of PVC and di(2-ethylhexyl) phthalate (DEHP) to explore their effects on hatchability and mortality of zebrafish (Danio rerio) embryos and toxicity to oxidative stress and cardiac development in zebrafish larvae. Herein, PVC-MPs slowed down the hatching rate of zebrafish embryos and induced the death of zebrafish, while DEHP could slow down the induced of death, it had no effect on hatching rate. The PVC-MPs/DEHP single pollution could induce the reactive oxygen species (ROS) and activated the antioxidant defense signaling pathway, while the compound group showed the level of feedback autoregulation of NF-E2-related factor 2 (Nrf2) signaling pathway. The single pollution also could inhibit the expression of genes related to cardiac development, while the combined pollution showed an antagonistic effect. This study provided a theoretical basis for the ecotoxicology and biomonitoring of MPs in the natural state.

Chronic exposure to polyvinyl chloride microplastics induces liver injury and gut microbiota dysbiosis based on the integration of liver transcriptome profiles and full-length 16S rRNA sequencing data

Microplastics (MPs) have become harmful environmental pollutants, and their potential toxicity to organisms has attracted extensive attention. However, the effects of polyvinyl chloride MPs (PVC-MPs) on the liver and their associated mechanism in mice remain obscure. Here, male mice were exposed to 2-μm PVC-MPs (0.5 mg/day) for 60 days and then sacrificed, and their liver, blood and gut feces were subsequently collected for testing. The liver tissue and fecal samples were subjected to RNA sequencing and full-length 16S rRNA sequencing analysis, respectively. Our results showed that the levels of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST) in the mice exposed to PVC-MPs were markedly higher than those in the control group, implying hepatic injury, as evidenced by hepatic histopathological changes. Moreover, the serum and hepatic triglyceride (TG) and total bile acid (TBA) levels were decreased after exposure to PVC-MPs. The RNA sequencing of mouse liver tissue identified a total of 1540 differentially expressed genes (DEGs) associated with 47 pathways, including the lipid metabolic pathway, oxidative stress, and the phosphoinositide 3 kinase (PI3K)/protein kinase B (Akt) signaling pathway, and these DEGs were enriched in the mouse livers. The full-length 16S rRNA sequencing analysis of the gut microbiota in mouse fecal samples revealed that PVC-MPs exposure decreased the relative abundance of probiotics and increased the abundance of conditionally pathogenic bacteria. In conclusion, chronic PVC-MPs exposure causes hepatotoxicity and gut microbiota dysbiosis in mice, and these findings provide new insight into the potential risks of PVC-MPs to human health.

Effects of polyvinyl chloride microplastics on reproduction, oxidative stress and reproduction and detoxification-related genes in Daphnia magna

The drastic increase of microplastics (MPs) in aquatic environment has become a serious threat to marine and freshwater ecosystems. However, little information is available regarding the potential detrimental effects of polyvinyl chloride microplastics (PVC-MPs) on aquatic organisms. This study investigated the changes of reproduction parameters, oxidative stress and the expression of reproduction and detoxification-related genes in Daphnia magna after exposed to 2 ± 1 and 50 ± 10 μm PVC-MPs. The results showed that chronic exposure to 2 ± 1 μm PVC-MPs prolonged days to the first brood, increased total number of broods per female and frequency of molting per adult, decreased offspring number at first brood and total number of offspring per female in D. magna. Moreover, 2 ± 1 μm PVC-MPs also disturbed the activities of SOD and CAT, increased GSH and MDA levels. The expression of Vtg, SOD, CAT, CYP314 and CYP360A8 genes also exhibited different response patterns depending on exposure time. Furthermore, 50 ± 10 μm PVC-MPs decreased offspring at first brood and Vtg mRNA level, increased the transcription levels and activities of SOD and CAT. These results suggest that the presence of PVC-MPs in aquatic environment may cause reproduction toxicity by disrupting the reproduction and detoxification-related genes expression and inducing oxidative stress in D. magna.

Microplastics with cadmium inhibit the growth of Vallisneria natans (Lour.) Hara rather than reduce cadmium toxicity

Microplastics and heavy metals are discharged into a freshwater environment either directly or via surface runoff and are largely deposited in sediments, posing risks to aquatic organisms. Few studies have thus far been devoted to the interaction of microplastics and heavy metals in sediments. Whether microplastics can affect the toxicity and accumulation of heavy metals in submerged macrophytes remains unclear. We evaluated the effects of polyvinyl chloride microplastics (PVC-MPs) and cadmium (Cd) exposure levels (0, 5, 15, and 25 mg) on Vallisneria natans (Lour.) Hara grown in sediment in a microcosm experiment for 14 d. In this study, PVC-MPs decreased the fresh weights of V. natans in the absence of Cd and markedly reduced the fresh weights at 5 and 15 mg Cd exposure levels. Moreover, PVC-MPs substantially increased the malondialdehyde (MDA) content of V. natans leaves at a Cd exposure of 25 mg. However, the PVC-MPs neither reduced the Cd concentration nor independently increased the antioxidant enzyme activities of the plants. These findings indicate that microplastics can independently, or jointly with a Cd contaminant, inhibit the growth of submerged macrophytes rather than reduce Cd toxicity. To our knowledge, this study is the first to evaluate the effects of microplastics and Cd exposure in sediments on the growth and physiological traits of submerged macrophytes, which could provide important implications for the interaction and future risk assessment of microplastics and heavy metals in sediments of freshwater ecosystems.

The interactions between microplastic polyvinyl chloride and marine diatoms: Physiological, morphological, and growth effects

Microplastics are identified as a great threat to marine environments. However, knowledge of their impacts on phytoplankton, especially for the diatoms is scarce. Herein, the effects of different polyvinyl chloride (PVC) microplastic concentrations and contact times (24, 48, 72 and 96 h) on the Fv/Fm and cell density of Phaeodactylum tricornutum (B255), Chaetoceros gracilis (B13) and Thalassiosira sp. (B280) were investigated to evaluate the toxic effects of microplastics on marine diatoms. The effects of PVC microplastics on the morphology of the diatoms was observed by SEM. The order of sensitivity to 1 μm PVC microplastics among three marine diatoms was B13 > B280 > B255, showing that the toxic effects varied with different microalgae species. Furthermore, the presence of a siliceous cell wall played a minimal role in protecting cells from the physical attack of PVC microplastics, with no significant difference from the common cell wall. PVC microplastics caused dose-dependent adverse effects on three marine diatoms. High PVC concentrations (200 mg/L) reduced the chlorophyll content, inhibited Fv/Fm, and affected the photosynthesis of three marine diatoms. The PVC microplastics adsorbed and caused physical damage on the structure of algal cells. Interactions between PVC microplastics and diatoms may be the probable reason for the negative effects of PVC on diatoms.

Long-Term Effects of Polyvinyl Chloride Microplastics on Anaerobic Granular Sludge for Recovering Methane from Wastewater.

Polyvinyl chloride microplastics (PVC-MPs) are emerging contaminants affecting biological wastewater treatment processes. However, most of the previous studies mainly focused on their short-term impacts on floc sludge, with little work being conducted to explore their potential effects on more complex anaerobic granular sludge (AGS), which has been widely used for high-strength organic wastewater treatment. In this paper, the long-term effects of PVC-MPs on AGS were investigated via continuous feeding tests that are representative of real wastewater treatment processes. The results of a continuous 264 days test showed that the prolonged exposure of PVC-MPs at 15-150 MPs·L-1 significantly (p = 7.86 × 10-37, 3.44 × 10-43, and 5.29 × 10-46) inhibited the COD removal efficiency of AGS by 13.2%-35.5%, accompanied by a 11.0%-32.3% decreased production of methane and 40.3%-272.7% increased accumulation of short-chain fatty acids (SCFAs). In addition, the PVC-MPs exposure suppressed the secretion of extracellular polymeric substances (EPS), causing AGS and the inside microorganisms to lose the protection of EPS, thereby resulting in granule breakage and decreased cells viability. Aligning with the deteriorated performance, the long-term exposure of PVC-MPs reduced the total microbial populations and the relative abundances of key methanogens and acidogens. A toxicity mechanism assessment revealed that the negative impacts induced by PVC-MPs are mainly attributed to the toxic leachate and excess oxidative stress.

Effect of Polyvinyl Chloride Microplastics on Bacterial Community and Nutrient Status in Two Agricultural Soils.

Knowledge of the influence of microplastics on soil microbiome and nutrients is important for understanding the ecological consequences of microplastic pollution in terrestrial ecosystems. In this study, we investigated whether polyvinyl chloride (PVC) microplastic pollution at environmentally relevant concentrations would affect soil bacterial community and available nitrogen/phosphorus content. The results showed that although PVC microplastics at 0.1% and 1% levels did not have a significant effect on overall bacterial community diversity and composition in soil over the course of 35 days, a number of bacterial genera were significantly reduced or enriched by the presence of microplastics. Potentially due to their effect on certain functional groups, microplastics caused a significant change in soil available P content. It is noteworthy that, depending on soil type, pollution level and plasticizer presence, contrasting effects of microplastics may be observed. Further research is definitely warranted to gain a clearer picture of the threats posed by microplastic pollution in soil environments.

Behavior of nitrogen, phosphorus and antibiotic resistance genes under polyvinyl chloride microplastics pressures in an aerobic granular sludge system

Polyvinyl chloride (PVC) is the common microplastics (MPs) detected in wastewater treatment plants causing harmful effects on organisms. However, the effects of PVC MPs on an aerobic granular sludge (AGS) system is not clear. In present study, the behavior of nitrogen, phosphorus and antibiotic resistance genes (ARGs) under PVC MPs pressure in an AGS system was investigated. Results showed PVC MPs promoted the phosphorus release and uptake of polyphosphate accumulating organisms (PAOs) and reduced total inorganic nitrogen removal efficiency. Extracellular polymeric substances were positively linked to PVC MPs. Moreover, PVC MPs increased the abundance of PAOs and Nitrospira, but decreased the abundance of denitrifying bacteria. The propagation of intI1 and tetE could be facilitated by PVC MPs, and the main antibiotic resistance mechanism of AGS system was the efflux pump. Chloroffexi, Actinobacteria, Bacteroidetes and Nitrospirae hold higher tolerance to PVC MPs than Proteobacteria. Network analysis showed PVC MPs positively correlated with some Accumulibacter clades and ARGs. This study indicated PVC MPs inhibited nitrogen removal and benefited ARGs propagation, which need to cause great concern.

Polyvinyl Chloride Microplastics Affect Methane Production from the Anaerobic Digestion of Waste Activated Sludge through Leaching Toxic Bisphenol-A.

The retention of polyvinyl chloride (PVC) microplastics in sewage sludge during wastewater treatment raises concerns. However, the effects of PVC microplastics on methane production from anaerobic digestion of waste activated sludge (WAS) have never been documented. In this work, the effects of PVC microplastics (1 mm, 10-60 particles/g TS) on anaerobic methane production from WAS were investigated. The presence of 10 particles/g TS of PVC microplastics significantly ( P = 0.041) increased methane production by 5.9 ± 0.1%, but higher levels of PVC microplastics (i.e., 20, 40, and 60 particles/g TS) inhibited methane production to 90.6 ± 0.3%, 80.5 ± 0.1%, and 75.8 ± 0.2% of the control, respectively. Model-based analysis indicated that PVC microplastics at >20 particles/g TS decreased both methane potential (B0) and hydrolysis coefficient (k) of WAS. The mechanistic studies showed that bisphenol A (BPA) leaching from PVC microplastics was the primary reason for the decreased methane production, causing significant ( P = 0.037, 0.01, 0.004) inhibitory effects on the hydrolysis-acidification process. The long-term effects of PVC microplastics revealed that the microbial community was shifted in the direction against hydrolysis-acidification and methanation. In conclusion, PVC microplastic caused negative effects on WAS anaerobic digestion through leaching the toxic BPA.