Effect Of Polyherbal Formulation Research Articles

GC-MS and Antioxidative Effects of Poly Herbal Formulation of Persea americana Seed Against Ethanol-Induced Gastric Ulcer in Rats

GC-MS and Antioxidative Effects of Poly Herbal Formulation of Persea americana Seed Against Ethanol-Induced Gastric Ulcer in Rats

Effect of Polyherbal Formulation (PHF) and its comparative study with other treatment in different types of dermatitis (Eczema) in experimental animals

Effect of Polyherbal Formulation (PHF) and its comparative study with other treatment in different types of dermatitis (Eczema) in experimental animals

Effect of Polyherbal Formulation (PHF) and its comparative study with other treatment in different types of dermatitis (Eczema) in experimental animals

Atopic dermatitis (AD) and allergic contact dermatitis are skin disorders that are a type of eczema. AD is a childhood disorder & allergic contact dermatitis. When the skin touches irritating bacteria, the skin inflammation causes itching and redness. The Poly Herbal Formulation uses this content: chaulmoogra oil 10%, neem oil 8%, and Karanja oil 30%, then made into an ointment. They are using this ointment topically for mice's skin after 30 days of redness, itching, dryness, and inflammation necessary for the basic treatment. This paper provides a different study of eczema AD & contact allergic dermatitis by using a polyherbal formulation.

Effect of polyherbal formulation for immunomodulation in immunosuppressed mice

Effect of polyherbal formulation for immunomodulation in immunosuppressed mice

Anti-diarrhoeal activity of a polyherbal formulation in rats and elucidation of its cellular mechanisms.

The present study was aimed to study anti-diarrhoeal activity of a polyherbal formulation (PHF) in rats and elucidate its mechanism of action. Anti-diarrhoeal activity of PHF was investigated using castor oil-induced diarrhoea, small intestinal transit and enteropooling models in rats. PHF was tested at 75, 150 and 300 mg/kg rat body weight. Loperamide was used as a reference control for in vivo studies. Anti-secretory action was evaluated against heat labile enterotoxin (from Escherichia coli) induced secretion in rat ileal loop model. The effect of PHF (12.5-100 µg/ml) on cAMP-dependent secretory activity was investigated against forskolin-induced cAMP release in HT-29 cells. PHF demonstrated significant (p≤0.05) anti-diarrhoeal activity by increasing the time for first faecal drop and inhibited diarrhoeal episodes by 43, 58 and 60% at 75, 150 and 300 mg/kg body weight, respectively in a dose-dependent manner. Also, the intestinal transit was inhibited upto 33% and the weight of secretory contents induced by castor oil was significantly reduced by PHF, approximately 29% in enteropooling assay. On the other hand, the intestinal loop instilled with PHF and enterotoxin from E. coli demonstrated 61% inhibition of fluid accumulation as compared to loop instilled with enterotoxin only. In vitro studies indicated that PHF inhibits cAMP release in HT-29 cells corroborating the anti-secretory effects observed in aforesaid studies. The results suggest that the PHF possesses anti-diarrhoeal activity, evident through reduced faecal output, decreased intestinal transit and anti-secretory activities.

Effect of poly herbal formulation “Karisalai chooranam ” against detrimental effects of psychological stress

Background: The association of psychiatric illness with altered cytokine profile and changes in neurobehavior are well known. “Karisalai chooranam” (KSC) is a polyherbal preparation, contains equal proportion of six ingredients namely Eclipta prostrata Linn., Acalypha indica Linn., Sphaeranthus indicus Linn., Indigofera tinctoria Linn., Centella asiatica Linn., and Wedelia chinensis. Restraint stress is reported to be a model for psychological stress. It was hypothesized that KSC might protect against restraint stress-induced detrimental effects. Objectives: The objective is to evaluate the efficacy of KSC against detrimental effects of psychological stress. Materials and Methods: Male Wistar albino rats were subjected to restraint stress (6 h/day, 21 days). At the end of stress, rats were subjected to elevated plus maze (EPM) and Moris Water Maze studies. Blood was collected and examined for interleukin-6 (IL-6), IL-10 by Enzyme-Linked Immuno Sorbent Assay. Results: Restraint stress produced raise in the plasma IL-6 and decrease in the IL-10 level. Abnormalities in memory and anxiety level were seen. The effects of KSC in preventing detrimental effects on spatial recognition skill and reference memory were observed in Morris water maze analysis. In EPM trail, the anxiety developed due to restraint stress was prevented by the KSC. Conclusion: KSC was found to have action against psychological stress-induced neurobehavioral changes and stress-induced alteration in cytokines which play important roles in the development of psychological stress.

Hypoglycemic and hypolipidemic effects of a poly herbal formulation in streptozotocin induced diabetic rats

Diabetes mellitus is a chronic disease with several comorbidities. Currently used synthetic medicines have various side effects. Herbal medicines are being widely used for management of diabetes. The present study was conducted to evaluate the effect of poly herbal formulation (PHF) (mixture of Citrullus colocynthis, Syzygium cumini and Holarrhena antidysenterica) in diabetic rats. We evaluated enzyme inhibition activity of PHF by ELISA and anti-hyperglycemic action was assessed in streptozotocin induced diabetic rats. Animals were divided into 5 groups, i.e., blank, negative control, standard control and PHF 250 and 500 mg/kg dosage respectively. Blood glucose level assessed through one touch method blood glucose monitoring system. The statistical analysis was performed by using one-way ANOVA p value of ≤0.05 was considered significant. Results of study indicated that PHF sufficiently inhibited DPPH at 0.5 mg/mL and serial dilutions with IC50 of 197 µg/mL while α- glucosidase inhibition assay showed marked inhibition of PHF at 0.5 mg/mL and serial dilutions with IC50 of 235 µg/mL. Oral intake of PHF reduced blood glucose level, serum triglyceride and cholesterol levels in wistar albino rats in dose dependent manner. Considering the multidirectional effects of PHF, it is concluded that PHF may be a therapeutic option in management of diabetes.

43 - The beneficial potential of polyherbal formulation in protecting against streptozotocin-nicotinamide-induced cardiomyopathy through Nrf2-regulated anti-inflammation, anti-oxidant and anti-fibrosis in C57BL/6J mice

The study investigates the effects of methanolic extracts of Terminalia paniculata(MTP), Piper nigrum (MPN), Gymnema sylvetsris (MGS)and a combination of their poly herbalformulation (PHF) againststreptozotocin (STZ)-nicotinamide (NAD)-induced diabetic cardiomyopathy (DCM) in C57BL/6J mice. MTP (200 mg/kg body weight (BW)), MPN (200 mg/kg BW), MGS (100 mg/kg BW) and PHF (500 mg/kg BW) were administered orally for 45 days to diabetic mice, and their biochemical parameters and cardiac markers (AST, ALT, LDH, troponin and cardiotroponin) as well as antioxidant profile were estimated. Heart antioxidant mRNA expressions, such as SOD, CAT, GpX, GST, GR, Nrf2, NF-kB, GCLC, GCLM by RT-PCR and their protein expressions by western blot were observed along with histopathological changes in the aortain control and experimental mice, and these effects were compared with gliclazide. Treatment with MTP, MPN, MGS, PHF and gliclazide for 45 days resulted in significant modulations in the levels of biochemical parameters, cardiac marker enzymes and oxidative markers. We also found PHF effectively altered the expressions of mRNA levels and protein levels of antioxidants, anti-inflammatory and anti-fibrosis in diabetic mice. The histopathological alteration of aorta reduced most of the pathological alterations with PHF treatment. The effect of PHF was more pronounced than that of the MTP, MPN, and MGS and brought back all the parameters to nearnormal. Thus, the present findings suggest that this combination of PHF may be considered as an effective therapeutic agent for the treatment of DCM by regulation of Nrf2-mediated oxidative stress, anti-inflammatory and anti-fibrosis markers, and restoration of cardiac damage.

Hepatoprotective effect of Poly herbal formulation containing indigenous medicinal plants against various hepatotoxic agents in rats

Hepatoprotective effect of Poly herbal formulation containing indigenous medicinal plants against various hepatotoxic agents in rats

In vivo anti oxidative effect of polyherbal formulation of flax seed, fenugreek and jamun seed on streptozotocin-nicotinamide induced diabetic rats

In vivo anti oxidative effect of polyherbal formulation of flax seed, fenugreek and jamun seed on streptozotocin-nicotinamide induced diabetic rats

Antihyperlipidemic Effect of Polyherbal Formulation (PHF) in High Fat Diet Induced Hyperlipidemia

The present study was undertaken to design and assess the influence of polyherbal formulation (PHF) on lipid profile in hyperlipidemic rats. The chronic hyperlipidemia was induced in wistar albino rats of either sex using high fat diet i.e. mixture of coconut oil and vanaspati ghee (2:3 v/v). The rats were fed with high fat diet for a period of 08 consecutive weeks. After the treatment with PHF, the following biochemical parameters were evaluated -serum total cholesterol, triglycerides, low density lipo-protein cholesterol (LDL), very low density lipo-protein cholesterol (VLDL) and serum high density lipo-protein cholesterol (HDL). The treatment with PHF (100 and 200mg/kg, p.o.) significantly reduced the hyperlipidemia, by decreasing the levels of total serum cholesterol, LDL, VLDL and by increasing the HDL in a dose dependent manner, when compared to the control vehicle and standard drug Atorvastatin. The results demonstrated that the PHF possess significant antihyperlipidemic activity.

Preliminary evaluation of hepatoprotective potential of the polyherbal formulation.

Aim:The aim of this study was to investigate the antioxidant and hepatoprotective effects of the polyherbal formulation (PHF)containing Cajanus cajan (L.)Millsp., Lawsonia inermis L. Linn, Mimosa pudica L., Uraria picta (Jacq.)DC. and Operculina turpethum (L.)Silva Manso on carbon tetrachloride (CCl4)induced acute liver damage in albino rats.Materials and Methods:The groups of animals were administered with PHF at the doses 100, 200 and 400 mg/kg b.w. (per oral [p.o.])once in a day for 7 days and at day 6th and 7th the animals were administrated with Carbon tetrachloride (1.0 mL/kg b.w. 50% v/v with olive oil,; p.o.). The effect of PHF on serum glutamine pyruvate transaminase (SGPT), serum glutamine oxaloacetate transaminase, alkaline phosphatase (ALP)and total bilirubin were determined in CCl4 - induced hepatotoxicity in rats. Further, the effects of PHF on glutathione (GSH), superoxide dismutase (SOD)level and lipid peroxidation (LPO)activity were also investigated.Results:The results demonstrated that PHF (400 mg/kg b.w.)significantly reduces the CCl4 induced increase in level of serum SGPT, serum ALP and total bilirubin. PHF (400 mg/kg b.w.)prevents the depletion level of GSH and decrease in the activity of SOD in CCl4 -induced liver injury in rats. In addition, PHF also showed a significant decrease in the LPO levels signifying the potent antioxidant activity.Conclusion:All our findings suggest that PHF could protect the liver cells from CCl4 - induced liver damages and the mechanism may be through the anti-oxidative effect of PHF.

Pharmacological investigation of Polyherbal formulation on Carbon tetrachloride (CCl4)-induced liver damage in wistar rats

Objective: To investigate effect of Polyherbal formulation on Carbon tetrachloride (CCl 4 )-induced liver damage in wistar rats. Methods: Wistar albino rats weighing 180-230 g either sex were used. The selected animals were divided in to four groups where each group consisted of six animals. Experimentally liver damage was produced by intra-peritoneal administration of CCl 4 and olive oil mixture (1:1 v/v) (1 mL/kg, once daily, i.p.) for 7 days. Test Drug, Polyherbal formulation was administered orally for 7 consecutive days at 3 mL/kg, once daily. On 8 th day, Blood samples were collected to evaluate different serum biochemical parameters like Aspartate aminotransferase (AST), Alanine aminotransferase (ALT), Alkaline phosphatase (ALP), Total bilirubin and Total protein. Liver from animals of each group was dissected out for histopathological examination. Statistical analysis: Statistical calculation were done by analysis of variance (ANOVA) followed by post hoc Dunnetts test, with significant level of p<0.05. Results and dDiscussion: Polyherbal formulation showed significant effect on activity levels of serum AST, ALT, ALP and total bilirubin level while comparing test group to disease control group. It also showed significant elevation in decreased level of serum total protein. Pre-treatment of Polyherbal formulation restored the hepatic architecture and protected the liver tissue from fatty degenerative changes by preventing the toxic chemical reaction induced by CCl 4 . Conclusion: Finding of this study concludes that Polyherbal formulation (Vasuliv Syrup) has promising hepatoprotective activity against CCl 4 -induced liver damage. It can be employed as safe and effective treatment for hepato-toxicity or liver damage.

AN AYURVEDIC POLYHERBAL FORMULATION PDBT FOR DYSLIPIDEMIA AND PREVENTION OF CORONARY ARTERY DISEASE (CAD) IN PRE-DIABETIC INDIVIDUALS

Pre - diabetes is a ‘ gre y area ’ between normal and diabete s. Various studies have shown that pre - diabetic subjects who developed diabetes had higher triglyceride and cholesterol values at baseline. At the time of diagnosis of diabetes around half of the patients will show s ome evidence of coronary artery disease. Treating dyslipidaemia in pre diabetes condition can not only reduce the conversion rate to diabetes but also occurrence of coronary artery disease ( CAD) . The Purpose of present study was to see whether an Ayurvedic polyherbal formulation PDBT which conta ins water extracts of Guduchi ( Tinospora cordifolia ) , Vijaysar ( Pterocarpus marsupium ), Gudmar ( Gymnemma sylvestre ) , Karvellak ( Momordica charantia ) and Shunthi ( Zinziber officinale ) can reduce mild to moderate dyslipidaemia in Pre - diabetic state as compar ed to placebo. A double blind placebo controlled (Randomized Controlled Trial) RCT was conduc ted on 100 patients ( 50 in each group) having m ean age 48.78 ± 10.098 ; for 6 months to see the effect of polyherbal formulation as compared to placebo in pre - di a be tic individuals. A diagnostic criterion for pre - diabetes was set according American Diabetic Association (ADA) 2000. Cholesterol , High Density Lipoprotein ( HDL ) , was done with Wyebenga and Pileggi ’s method / Bio - lab Kit. Triglycerides were estimated by enz ymatic Kit method. Blood sugar level (BSL) was calculated by Glucose oxidase - peroxidase ( GOD - POD ) method. Low Density lipoprotein (LDL), Very Low Density Lipoprotein (VLDL) was calculated by formula. These investig ations were done before treatment (BT) an d after treatment (AT) . Unpaired T and paired T test were applied by INSTAT 2 software as a test of significance. Mean Cholesterol before treatment and after treatment 200.04 ± 41.6 and 172.09 ± 42.29, Mean HDL before treatment and after treatment 44.544 ± 10.517 and 47.30 ± 8.35, Mean LDL 116.37 and 92.91. Triglycerides before treatment and A. T. 160.16 ± 132.02 in PDBT treated group. W hile in placebo treated group these val ues were increased from 170.53 ± 32.05 to 191.62 ± 36.75 , for Cholesterol before tr eatment and after treatment . LDL increased from 92.85 ± 31.19 to 99.139 for LDL. While for triglycerides values increased from 165.75 ± 44.33 to 211.18 ± 46.7.

Evaluation of hypoglycemic and anti –diabetic activity of poly‐herbal formulation in normal and streptozotocin induced diabetic rats

The present study was focused to evaluate the hypoglycemic and antidiabetic activity of Maddi Dicare the poly ‐herbal formulation, in both normal and experimentally induced hyperglycemic (Streptozotocin ) rats. Different doses of poly herbal formulation (50, 100 and 200 mg/kg) was administered in rats orally. At the end of the treatment period, the serum from normal and diabetic animals was subjected to the estimation of blood sugar, using glucose kits. Effect of Polyherbal formulation on glucose tolerance was also carried out. It was found to produce significant hypoglycemic activity in both normal and diabetic animals, which could be compared to glibenclamide (10 mg/kg). The Poly‐herbal formulation was found to increase the glucose tolerance in normal rats. The present study demonstrates that herbal formulation exhibits promising anti‐diabetic activity and helps to maintain good glycemic and metabolic control. It also proved hypoglycemic and antidiabetic activity in a dose dependent manner.

Effect of Polyherbal Formulation in Treatment of Poly Cystic Ovarian Syndrome (PCOS)

Poly cystic ovarian syndrome (PCOS) is a complex syndrome affecting 5 to 15% of the females of reproductive as well as premenopausal age. It involves various symptoms related to metabolism and reproductive system. According to Ayurveda it is a disorder of Artav-vah strotus caused by vitiation of Kapha and Vata. In this study a Polyherbal formulation prepared by Unijules life Sciences ltd. is evaluated on 36 patients suffering from various symptoms of PCOS for three months treatment duration. A highly significant result was observed in pain during menstruation, interval between two menses, follicular size and in decreasing the BMI i.e. in obesity also. Overall this study established a good product useful for PCOS without causing any unwanted effects.

Hepatoprotective effect of poly herbal formulation against various hepatotoxic agents in rats

Background:Individually Andrographis paniculata Nees. (Acanthaceae), Phyllanthus niruri Linn.(Euphorbiaceae) and Phyllanthus emblica Linn. single plant extracts have been reported to have hepatoprotective activity. However, literature survey shows that no sufficient scientific data has been publish on pharmacological evaluation of these plants in combined form.Method:Hepatoprotective activity of the polyherbal hepatoprotaective formulation (PHF)-containing spray-dried aqueous extracts of Andrographis paniculata Nees. (Acanthaceae), Phyllanthus niruri Linn. (Euphorbiaceae) and Phyllanthus emblica Linn. (Euphorbiaceae), was screened against paracetamol, carbon tetrachloride (CCl4), and ethanol-induced hepatic damage in rats. PHF was evaluated by measuring levels of serum marker enzymes like SGOT, SGPT, ALP, direct bilirubin (DB), and lactate dehydrogenase (LDH). The histological studies were also studied support the biochemical parameters. Silymarin was used as standard drug.Results:Administration of PHF (100 and 200 mg/kg p.o.) significantly inhibited paracetamol, CCl4 and ethanol-induced elevation levels of SGPT, SGOT, ALP, DB and LDH. A comparative histopathological study of liver exhibited almost normal architecture as compared to toxicant group.Conclusion:Results suggests that the hepatoprotective effects of PHF might be useful for liver protection due to combined action of all plant extracts along with their phytoconstituents.

Protective and curative effect of poly herbal formulation containing indigenous medicinal plants against various hepatotoxic agents in rats

ObjectiveTo evaluate the protective and curative potential of PHF, a polyherbal formulation, against two experimentally induced hepatotoxicity models in rats. MethodsHepatoprotective activity of the PHF containing three indigenous medicinal plants extracts Coccinia indica, Sida cordata and Scoparia dulcis, was screened against CCl4 and paracetamol induced hepatotoxicity in rats. ResultAdministration of hepatotoxins (CCl4 and paracetamol) shows significant morphological, biochemical and histopathological deteriorations in the liver of experimental animals. Pretreatment with PHF had significant protection against hepatic damage by maintaining the morphological parameters within normal range and normalizing the elevated levels of biochemical parameters (SGPT, SGOT, ALP and total bilirubin), which were evidently showed in histopathological study. ConclusionsThe PHF has highly significant hepatoprotective effect at 100 and 200 mg/kg, p.o. on the liver of all the two experimental animal models. The liver protection due to combined action of all plant extracts along with their phytoconstituents.

간기능 개선용 복합 식물 추출물(Hepa-1000)의 tert-butyl hydroperoxide(t-BHP)로 유도한 간세포 독성에 대한 보호 효과

본 연구에서는 간기능 개선제(Hepa-1000)의 t-BHP에 의해 유도된 산화적 스트레스에 대한 간세포 보호 효과를 확인하기 위해 인간 간암 세포주인 HepG2 세포를 이용한 세포실험으로 검증하였다. 간세포에 t-BHP로 유도되는 간세포의 산화적 손상에 대한 보호 효과를 측정한 결과, Hepa-1000을 처리시 대조군에 비해 유의적인 세포 생존율 증가치를 보이므로 간기능 개선제(Hepa-1000)가 t-BHP에 의한 간세포의 괴사에 대해 강한 보호 효과가 있음을 알 수 있었다. 또한 간기능 개선 효능을 확인하기 위해 세포 배양액 내의 GOT와 LDH의 활성도를 측정 시 t-BHP에 의해 간세포 손상이 확연하게 유발되었으며, 간기능 개선제(Hepa-1000)는 GOT와 LDH의 증가를 유의적으로 뚜렷하게 억제하였다. Hepa-1000의 t-BHP에 의해 유도된 산화적 스트레스에 대한 보호 효과를 세포막 손상 시 배양액 내의 세포 지질과산화 정도를 측정하는 MDA양을 측정하였을 때, Hepa-1000을 처리시 MDA의 양이 감소함을 관찰할 수 있었다. 이러한 결과들을 종합해 볼 때 간기능 개선제인 Hepa-1000은 간세포에서 t-BHP의 대사과정시 발생되는 free radical에 의한 세포내 지질과산화 현상을 방어하여, 세포막 손상 시 배양액으로 유출되는 GOT, LDH의 양을 감소시키므로 산화적 스트레스에 의한 간세포의 손상을 보호함으로써 인체에서 해독작용을 담당하는 조직인 간의 기능을 개선시키고 보호하는 효과를 나타내리라 사료된다.