Effects Of Photobiomodulation Research Articles

Effects of Photobiomodulation on Oral Mucositis: Visualization and Analysis of Knowledge.

This review article mapped and analyzed the most cited articles on the association of photobiomodulation (PBM) with oral mucositis (OM) and the evolution of clinical protocols in the area. A comprehensive search was performed on the Web of Science Core Collection (WoS-CC) database, leading to the extraction of information such as title, authors, abstract, journal name, number, average of citations, study design, year of publication, institutions, continents, countries, type of laser used, irradiated anatomical points, primary anti-cancer therapy, and laser parameters. Among those, clinical trials and literature reviews were the most common study designs. The main type of laser used was the InGaAlP diode, with a wavelength ranging from 630-660 nm, power going in 40-100 mW, and energy density ranging from 0.375-22 J/cm2. As for the anatomical sites irradiated by PBM, the cheek mucosa, upper and lower lips, lateral tongue, and bottom of the mouth stood out. This analysis highlights an increasing interest in PBM as a supportive treatment in cases of OM, as well as the evolution of the technique, types of laser devices, and protocols used.

Anti-inflammatory effect of green photobiomodulation in human adipose-derived mesenchymal stem cells.

Photo biomodulation (PBM) as a non-invasive and safe treatment has been demonstrated the anti-inflammatory potential in a variety of cell types, including stem cells. However, further investigations using different laser parameters combined with more accurate methods such as quantitative measurement of inflammatory gene expression at the mRNA level are still necessary. The aim of this study was to evaluate the effect of 532nm green laser on cell proliferation as well as expression of inflammatory genes in human adipose-derived mesenchymal stem cells (hADMSCs) using RNA sequencing (RNA-seq) technique and confirmatory RT-PCR. hADMSCs were cultured in DMEM low glocuse medium with 10% fetal bovine serum until the fourth passage. Cultured cells were divided in two groups: control group (no laser irradiation) and laser group, irradiated with 532nm laser at 44mJ/cm2 with an output power of 50 mW and a density of 6 mW/cm2, every other day, 7s each time. The cell viability was assessed using MTT assay 24h after each irradiation on days 3, 5, and 7 after cell seeding, followed by performing RNA-seq and RT-PCR. The MTT assay showed that PBM increased cell proliferation on day 5 after irradiation compared to day 3 and decreased on day 7 compared to day 5. In addition, gene expression analysis in hADMSCs using RNA-seq revealed down-regulation of inflammatory genes including CSF2, CXCL2, 3, 5, 6, 8, and CCL2, 7. These results indicate that 532nm PBM with the parameters used in this study has a time-dependent effect on hADMSCs proliferation as well as anti-inflammatory potential.

Short-Term Effects of Whole-Body Photobiomodulation on Pain, Quality of Life and Psychological Factors in a Population Suffering from Fibromyalgia: A Triple-Blinded Randomised Clinical Trial.

Fibromyalgia(FM) is a multifunctional chronic musculoskeletal pain condition characterised by sensory hypersensitivity. Photobiomodulation (PBM) has shown a positive impact on relieving pain; however, no studies to our knowledge have analysed a whole-body PBM intervention in subjects with FM. The aims of the study were to compare the effects of whole-body PBM with placebo PBM on pain, functionality and psychological symptoms in patients suffering from FM. Forty-two subjects were recruited from a private care practice. The design of the study is a randomised, triple-blinded, placebo-controlled clinical trial. Participants received 12 treatment sessions. Pain, quality of life, level of physical activity and psychological factors were assessed at baseline (T0), after session 6 (T1), after treatment (T2) and at 2-week (T3) follow-up. There were statistically significant differences in pain at 4weeks (p ≤ 0.001) (T2) and the 2-week follow-up (T3) (p ≤ 0.001). In relation to the quality of life, there were statistically significant improvements after session 6 (p ≤ 0.001) (T1), immediately after treatment (p ≤ 0.001) (T2) and at the 2-week (T3) follow-up (p ≤ 0.001). Kinesiophobia presented significant differences between groups immediately after treatment (p ≤ 0.001) (T2) and at the 2-week (T3) follow-up (p ≤ 0.001), with self-efficacy only showing significant differences between groups 2 weeks after the treatment (p = 0.01) (T2). There were no differences between groups when comparing pain catastrophising at any time. Whole-body PBM resulted in a significant reduction in pain and an improvement in quality of life in those participants suffering from FM after receiving 4weeks of treatment. Furthermore, psychological factors such as kinesiophobia and self-efficacy were also improved. Thus, a whole-body PBM treatment is presented as a possible new multifactorial treatment with potential benefits for those with FM and more studies are needed to corroborate our findings. ClinicalTrials.gov (NCT0424897).

Effects of systemic vascular photobiomodulation using LED or laser on sensory-motor recovery following a peripheral nerve injury in Wistar rats.

Peripheral nerve injury (PNI) is associated with considerable functional impairment. Photobiomodulation (PBM) has demonstrated positive effects regarding neuromuscular repair after PNI when applied locally to the nerve or injured muscle. However, the effects of systemic PBM with transcutaneous application over an important artery, which is also denominated vascular PBM (VPBM), remain unclear. The aim of the study was to compare the effects of VPBM with low-level laser (LLL) and light-emitting diode (LED) on gait, sensitivity and muscle morphology following a PNI. PNI was induced on Wistar rats using the sciatic nerve crushing technique. VPBM was performed over the rat's artery tail region with LED (850nm, 40mW, 3.2J) and LLL (780nm, 40mW, 3.2J). Gait functionality, mechanical (nociceptive) sensitivity, and morphology of the tibialis anterior muscle were evaluated at 7, 14, and 21days after injury. An improvement in functional gait was shown in the VPBM-LLL group in all periods. Motor sensitivity was found after 14days in the VPBM-LLL group. The left/right (L/R) muscle mass ratio revealed a reduction in muscle atrophy in the VPBM-LLL group at 7days. Muscle fiber diameter increased in the VPBM-LED group at 14days and increases in the cross-section area were found in the VPBM-LED and VPBM-LLL groups at 7days. VPBM with both light sources (LED and LLL) positively modulated functioning and neuromuscular recovery following sciatic nerve injury in rats, with more pronounced results when using LLL.

Systemic glucose levels are modulated by specific wavelengths in the solar light spectrum that shift mitochondrial metabolism.

Systemic glucose levels can be modulated with specific solar wavelengths that influence mitochondrial metabolism. Mitochondrial respiration can be modulated using light that shifts ATP production with exceptional conservation of effect across species, from insects to humans. Known wavelengths have opposing effects of photobiomodulation, with longer wavelengths (660-900 nm red/infrared) increasing ATP production, and 420 nm (blue) light suppressing metabolism. Increasing mitochondrial respiration should result in a greater demand for glucose, and a decrease should result in a reduced demand for glucose. Here we have tested the hypothesis that these wavelengths alter circulating glucose concentration. We first established an oral glucose tolerance test curve in a bumblebee model, which showed sustained increase in systemic glucose beyond that seen in mammals, with a gradual normalisation over eight hours. This extended period of increased systemic glucose provided a stable model for glucose manipulation. Bees were starved overnight and given a glucose load in the morning. In the first group glucose levels were examined at hourly intervals. In the second group, bees were additionally exposed to either 670 nm or 420 nm light and their blood glucose examined. Increasing mitochondrial activity with 670 nm light at the peak of circulating glucose, resulted in a significant 50% reduction in concentration measured. Exposure to 420nm light that retards mitochondrial respiration elevated systemic glucose levels by over 50%. The impact of 670 nm and 420 nm on mitochondria is highly conserved. Hence, different wavelengths of visible light may be used to modulate systemic metabolism bidirectionally and may prove an effective agent in mammals.

Mast cell stabilization: new mechanism underlying the therapeutic effect of intense pulsed light on rosacea.

Rosacea, a chronic inflammatory disorder of the facial skin, is effectively treated by intense pulsed light (IPL). To explore the potential molecular mechanism underlying the photobiomodulation effect of IPL for rosacea treatment. Skin samples from patients with rosacea were subjected to histological and immunohistological staining. Ten patients were followed up after IPL treatment using the VISIA® skin analysis system, and the severity was assessed. In vivo, skin changes in mice with rosacea-like inflammation induced by intradermal injection of 320μM LL-37 with or without IPL treatment were evaluated using L*a*b colorimetry as well as histological and immunological staining. In vitro, LL-37-stimulated mast cells (MCs) with or without IPL treatment were evaluated for protein expression of matrix metalloproteinase (MMP)-9, kallikrein-related peptidase 5 (KLK5), and cathelicidin using western blotting and qRT-PCR. Profound infiltration of inflammatory cells and evident MC degranulation were found in rosacea skin lesions. The expression of rosacea-related biomarkers and inflammatory cytokines was higher in lesional areas than in non-lesional areas, as demonstrated via immunochemical staining. In all patients, rosacea severity reduced after IPL therapy. In vivo, IPL alleviated inflammation in mice with rosacea-like inflammation, as demonstrated by the significantly decreased MMP-9, KLK5, and cathelicidin expression and reduced percentage of degranulating MCs. In vitro, IPL decreased MMP-9, KLK5, and cathelicidin expression in P815 cells, reducing the release of inflammatory cytokines and inhibiting rosacea-like inflammatory reactions. The photobiomodulation effect of IPL for rosacea treatment may inhibit MC degranulation and alleviate inflammatory reactions.

Long-Term Outcome of Photobiomodulation for Diabetic Macular Edema: A Case Report

Background: Macular edema is the major cause of decreased vision in patients with diabetes. Antioxidative and anti-inflammatory effects of photobiomodulation (PBM) have been reported with near-infrared irradiation. Objective: In this study, the efficacy and safety of PBM with near-infrared radiation in a patient with diabetic macular edema were assessed. Methods: We present the case of a 60-year-old man with diabetic macular edema responding to 670 nm light PBM alone. Results: After PBM, his vision improved and macular edema resolved on optical coherence tomography (OCT) findings without adverse events. In 16 months follow-up, visual acuity remained stable and OCT showed no evidence of recurrence of edema. Conclusions: Six hundred seventy nanometers light PBM can be potentially considered as a therapeutic method in diabetic patients with macular edema. Also, it provides a noninvasive and nonexpensive treatment in home.

A systematic review of the effect of photobiomodulation on the neuroinflammatory response in animal models of neurodegenerative diseases.

This systematic review examines the effect of photobiomodulation (PBM), the application of red to near infrared light on body tissues, on the neuroinflammatory response and oxidative stress in animal models of neurodegenerative diseases. The research question and search protocol were prospectively registered on the PROSPERO database. Neurodegenerative diseases are becoming evermore prevalent in the ageing populations across theWestern world, with no disease-modifying or neuroprotective treatment options being available. Hence there is a real need for the development of effective treatment options for patients. Inflammatory responses and oxidative stress within the central nervous system have a strong correlation with neuronal cell death. PBM is a non-invasive therapeutic option that has shown efficacy and promising effects inanimal models of neurodegenerative disease; many studies have reported neuroprotection and improved behavioural outcomes. To the best of our knowledge, therehas been no previous study that has reviewed theanti-inflammatory and the antioxidant effect of PBM inthe context of neurodegeneration. This review has examined this relationship in animal models of a range ofneurodegenerative diseases. We found that PBM can effectively reduce glial activation, pro-inflammatory cytokine expression and oxidative stress, whilst increasing anti-inflammatory glial responses and cytokines, and antioxidant capacity. These positive outcomes accompanied the neuroprotection evident after PBM treatment. Our review provides further indication that PBM can be developed intoan effective non-pharmacological intervention for neurodegenerative diseases.

Effects of Photobiomodulation and Low-Intensity Stretching on Delayed-Onset Muscle Soreness: A Randomized Control Trial.

Objective: This study aimed to investigate the effects of photobiomodulation (PBM), low-intensity stretching, and their combination on delayed-onset muscle soreness (DOMS) in the untrained population. The relationships between DOMS and muscle function and functional performance were also tested. Methods: Fifty-four participants were randomized into four groups. Eccentric exercise was used to induce DOMS. Each group received either no treatment, PBM, stretching or PBM combined with stretching at 24, 48, and 72 h postexercise. Pressure pain threshold (PPT), numerical rating scale (NRS), single-leg forward jump (SLFJ), and maximum isometric voluntary contraction (MIVC) were measured at baseline, 24, 48, 72, and 96 h after eccentric exercise. Between-group differences were tested using two-way repeated measures analysis of variance and the relationships between DOMS and MIVC, and SLFJ were examined using Pearson's correlation analysis. Results: The PPT at the vastus medialis and vastus lateral in the PBM combined with stretching group was significantly lower than that in control group at 72 h (p = 0.045) and 48 h (p = 0.037) postexercise. No significant between-group difference in PPT was found for the rest occasions. There was no significant between-group difference in NRS, MVIC, and SLFJ on any occasion (p ≥ 0.052). DOMS was not correlated with MIVC and SLFJ (p ≥ 0.09). Conclusions: PBM or low-intensity stretching did not affect DOMS and functional performance in untrained individuals. The combination of PBM and low-intensity stretching increased pain sensitivity and did not relieve soreness. The DOMS was not associated with either muscle function or functional performance.

A MINI REVIEW ON PHOTO- BIOMODULATION- Novel approach

This research is aimed to look at the existing literature on the effects of photobiomodulation (PBM) on a variety of medical illnesses with a high severity rate. Photobiomodulation, also known as non-thermal laser treatment, is an approaching field in the technology of medicine that involves exposing tissues to precise wavelengths of light to generate therapeutic effects. Photomedicine is based on the outcomes of coherent and noncoherent visible light on cells and tissues, like low-level lasers and light-emitting diodes (LEDs). This therapy method has an impact on cell function, proliferation, and migration, as well as tissue regeneration. Thus, this therapy's mechanism is particularly effective in displaying favorable benefits in neurological, pulmonary, inflammatory, dermatological, and wound healing illnesses and oncology. Using the biphasic dose-response technique, each ailment is addressed with a specific type of laser and within a defined wavelength range to produce the required effect of the therapy.

Photobiomodulation therapy on local effects induced by juvenile and adult venoms of Bothrops alternatus

Bothrops snake envenomation is characterized by severe local manifestations such as pain, edema, inflammation, hemorrhage, and myonecrosis. Furthermore, it is described that venom from juvenile and adult snakes may have differences in their composition that can lead to differences in the evolution of the clinical manifestation of the victim. Photobiomodulation (PBM) has been shown to be an effective adjuvant therapy to serum therapy to reduce the local effects induced by bothropic snake venom. This study evaluated the effect of PBM on the local reaction, after Bothrops alternatus snake venom (BaV) injection, in its juvenile (BaJV) and adult (BaAV) stages. Balb/C mice were injected with the juvenile or adult venoms of BaV or saline solution (control group). PBM at a wavelength of 660 nm, 100 mW, 0.33 W/cm2, 40 s, and a 0.028 cm2 beam was applied transcutaneous to a single point with a radiant exposure of 4 J/cm2, 30 min after venom injection. Edema, inflammatory infiltrate, hyperalgesia, and myonecrosis were analyzed. Both venoms induced significant edema and myonecrosis in the gastrocnemius muscle. Hyperalgesia in the mice paw and a prominent leukocyte infiltrate into the peritoneum were also observed. PBM significantly reduced all evaluated parameters. In conclusion, PBM treatment was effective in reducing the local effects induced by B. alternatus venom at different stages of snake development and could be a useful tool as an adjuvant treatment for bothropic envenomation.

Effect of Motexafin lutetium-mediated ultrasound-guided red laser photodynamic therapy on endothelial dysfunction using a new automatic ultrasound image processing software

Abstract Background As is well recognized, the majority of atherosclerosis start with an inflammatory process, resulting in endothelial dysfunction. Purpose In this study, we developed an experimental photosensitizer Motexafin lutetium (Lu-Tex)-mediated photodynamic therapy system, and investigated its effectiveness on endothelial dysfunction improvement in an animal model of balloon dilation injury, using a new automatic ultrasound image processing software. Methods Briefly, Golden Syrian hamsters' femoral artery underwent balloon dilatation injury. After four weeks, femoral arteries of the treatment group at the lesion region, treated using Motexafin lutetium - mediated fiber optic catheter-based red low level laser (742 nm, 150 J/cm2) photodynamic therapy. In order to evaluate endothelial-dependent relaxation, acetylcholine-mediated dilation (AMD) was measured during the infusion of acetylcholine at a rate of 0.5 μg/kg/min and endothelial independent relaxation was evaluated by measuring nitroglycerin mediated dilation (NMD) during the infusion of nitroglycerin at a rate of 5μg/kg/min. For off-line analysis, a computerized analysis method for evaluating instantaneous changes in the wall of the hamster femoral artery was used. For automatic measurement of the mean wall thickness and the lumen diameter from longitudinal B-mode ultrasound images, two algorithms, i.e., maximum gradient and dynamic programming were composed and implemented. Reference points and cost function were based on dynamic programming and maximum gradient, respectively. Results Results from computerized B-mode ultrasound image processing software showed significant differences in AMD between the treated and the non-treated hamsters (p<0.05), whereas there were no significant differences in NMD between the treatment and normal groups (p>0.05). Conclusion Cytotoxic and anti-inflammatory effect of Motexafin lutetium, induced by red laser photodynamic therapy accompanied by photobiomodulation effect of red laser, can cause to reduce the immune cells in intimal layer and improve the endothelial dysfunction via increasing the endothelial nitric oxide synthase function. Furthermore, it is concluded that the new automatic method enables accurate and repeated evaluation of the endothelial dysfunction using photodynamic therapy in this model. Funding Acknowledgement Type of funding sources: Public Institution(s). Main funding source(s): Mehrad Research Lab

The effect of photomodulation on fibroblast growth factor and the Ras/MAPK signalling pathway: a review.

Current therapies and technologies used to treat hard-to-heal diabetic wounds are limited to a 50% healing rate. The rise in the percentage of lower limb non-traumatic amputations in patients with diabetes has caused an increased demand for alternative, effective and safe treatment modalities. Photobiomodulation therapy (PBMT) utilises light to induce physiological changes and provide therapeutic benefits and has been shown to increase the healing of hard-to-heal wounds through the release of growth factors. The aim of this narrative review is to investigate the effect of photobiomodulation (PBM) on fibroblast growth factor (FGF) and the role of the Ras/MAPK signalling pathway in diabetic wound healing. Relevant journal articles were obtained through PubMed and Google Scholar. Experimental and clinical findings from the review show that PBM can stimulate the release of growth factors, including FGF, an essential cytokine in wound healing, and one which is present at lower concentrations in diabetic wounds. There is also activation of the Ras/MAPK signalling pathway. One mechanism through which healing may be stimulated by PBM is via the FGF-Ras/MAPK signalling pathway, although strong evidence under hyperglycaemic conditions is lacking.

Clinical and Histopathologic Evaluation of the Effect of Laser Photobiomodulation And Epicatechin in the Treatment of Radiation Induced Oral Mucositis in Rat Model

Clinical and Histopathologic Evaluation of the Effect of Laser Photobiomodulation And Epicatechin in the Treatment of Radiation Induced Oral Mucositis in Rat Model

Research progress of infrared light promoting wound healing

At present, current stimulation, ultra-sound, and light therapy have become effective methods to promote wound healing. Among them, infrared light is the most widely used method and is one of the important methods to promote wound healing. The therapeutic effect of infrared light on wounds is related to the effect of photobiomodulation on cells and molecules on the skin surface, but the mechanism by which photobiomodulation of infrared light promotes wound healing has not been fully elucidated. Therefore, it is necessary to study the action characteristics and the mechanism of photo-biomodulation of infrared light in promoting wound healing. This article reviews the effect of different types of infrared light on wound healing and the mechanism of infrared light in promoting wound healing.

Is There Evidence of Toxicity in Long-Term Photobiomodulation?

This editorial briefly discusses the issue of potential toxicity in the case of long-term photobiomodulation (PBM). Scientific articles from PubMed, Google Scholar, and the China National Knowledge Infrastructure were included in a heterogeneous search. There have been very few studies on the long-term effects of PBM, as well as its potential toxicity. There is no evidence that PBM is toxic when used at therapeutic or imaging doses. There are no such concerns with intracranial PBM application (e.g., optical fiber) in either animal or human experiments.

Analysis of decontamination of pressure injury through blue light - randomized trial clinical study

Objective: to analyze the effects of photobiomodulation using the Light-emitting Diode (LED), operating at wavelength 420 +/- 20 nm (Violet-Blue) in pressure decontamination (PI). Methods: The sample consisted of 36 patients, 24 of whom met the inclusion criteria and were randomized into 3 treatment groups, where three different photobiomodulation We used a Cosmedical® LED plate Cicatrillux (figure 1); containing 36 LEDs, 420 +/- 20 nm, optical diameter 10 mm +/- 2, optical output 2-5 mW, device energy 106J, radiant exposure 3.8 J / cm2, irradiance 6.4 mW / cm2 whit energy total Joules were applied in each, 53J (5 min) 106J (10 min) and 159J (15 min) respectively. Results: The analysis of the results was based on the counting of Colony Forming Units (UFC), as well as characterization of the lesions using the National Pressure Ulcer Advisory Panel (NPUAP) and severity by the Pressure Ulcer Score Healing (PUSH) scale. The mean age of the study patients was 72 years, most of the pressure lesions were in the sacral region (70%), 62% female patients, 79% of the lesions were located in the sacral region and 54% were stage III. Conclusion: The analysis of the results showed that the photobiomodulation of lesions at wavelengths of 420 nm to reduce contamination was not effective at the doses applied in the three study groups because, despite a slight reduction in CFU count in the time of 159J, was statistically significant.

Effects of photobiomodulation on mitochondrial function in diabetic adipose-derived stem cells in vitro

Herein are reported the effects of photobiomodulation (PBM) on adenosine triphosphate (ATP) and reactive oxygen species (ROS) quantification and mitochondria membrane potential (MMP) of the mitochondria of diabetic adipose-derived stem cells (ADSCs) in vitro. Additionally, the expression of PTEN-induced kinase 1 (PINK1) and RBR E3 ubiquitin-protein ligase (PARKIN) genes, which are involved in mitochondrial quality, were quantified.First, type one diabetes was induced in 10 rats. The rats were then kept for 1 month, after which fat tissue was excised from subcutaneous regions, and stem cells were selected from the fat, characterized as ADSC, and cultivated and increased in elevated sugar conditions in vitro; these samples were considered diabetic-ADSC. Two groups were formed, namely, diabetic-control-ADSC and PBM–diabetic-ADSC. ATP, ROS quantification, and MMP of mitochondria of diabetic ADSCs in vitro were measured, and the expression of PINK1 and Parkin genes was quantified in vitro.The results revealed that PBM significantly increased ATP quantification (p = 0.05) and MMP activity (p = 0.000) in diabetic-ADSCs in vitro compared to the control diabetic-ADSCs; however, it significantly decreased ROS quantification (p = 0.002) and PINK1(p = 0.003) and PARKIN gene expression (p = 0.046) in diabetic-ADSCs.The current findings indicate for the first time that PBM has the potential to maintain the function and quality of mitochondrial diabetic-ADSCs by significantly increasing ATP quantification and MMP activity in diabetic-ADSCs in vitro while significantly decreasing ROS quantification and PINK1 and PARKIN gene expression, making PBM an attractive candidate for use in improving the efficacy of autologous stem cell remedies for diabetic patients with infected diabetic foot ulcers.

Vascular photobiomodulation increases muscle fiber diameter and improves the gait during compensatory hypertrophy of plantar muscle in rats.

The local photobiomodulation (LPBM) has demonstrated positive effects during compensatory hypertrophy (CH) in skeletal muscle as a response to an overload. The aim was to compare the effects of the transcutaneous vascular photobiomodulation (VPBM) and the LPBM on muscle fiber size, gait functionality, and on mechanical sensitivity during the CH model in rats. VPBM was administered over the rat's main tail vein and LPBM was applied over the plantar muscle region. VPBM induced an increase in muscle fiber diameter and cross-sectional area (CSA) after 7 days. At 14 days, an increase in the fiber diameter was found in both irradiated groups. The VPBM and LPBM promoted the reestablishment of normal gait evaluated by the sciatic functional index after 14 days. No changes were found in the mechanical (nociceptive) sensitivity in VPBM and LPBM groups in comparison to the CH group but there was an increase in the nociceptive sensitivity in the CH groups in comparison to the control after 7 and 14 days. In conclusion, both PBM, vascular and local, were able to improve the muscle size and gait during the CH process with more pronounced effects when irradiation was performed systemically (VPBM).

Photodynamic therapy and Photobiomodulation as adjuvant treatment of oral and facial manifestations in patients with COVID-19 hospitalized in the intensive care unit: a case series

Orofacial injuries resulting from Coronavirus-19 (COVID-19) are reported in the current literature. The etiology of these manifestations is still unknown, but them seem to have an association between the prone position and patient’s immunosuppression. Clinical features range from nonspecific ulcers to vesicles that may mimic viral infections. The search for a clinical protocol for the cure of these lesions is necessary. Thus, some studies about photobiomodulation, a low-level light therapy with regenerative tissue action, associated with antimicrobial photodynamic therapy, as a promising adjuvant treatment. Therefore, the present study aims to report a series of cases where photobiomodulation and photodynamic therapy were used in patients positive for COVID-19 and presented orofacial lesions during the time of hospitalization in the intensive care unit. In all cases, tissue regeneration was complete, demonstrating the effectiveness of photobiomodulation and photodynamic therapy as adjuvant therapy associated with the use of medicines.