This study discusses fatigue properties of low carbon steel under multiaxial non-proportional loading and an evaluation of failure life. Multiaxial fatigue tests under non-proportional loading with various stress amplitudes were carried out using a hollow cylinder specimen in low and high cycle regions at room temperature. In the test, three types of strain/stress path were employed. They are a push-pull, a reversed torsion and a combined push-pull and reversed torsion loadings in which stress amplitudes used were constant and random. This study evaluates an effect of non-proportional loading on fatigue life in the high cycle fatigue region to discuss the applicability of ΔεNP proposed by Itoh et al. on life evaluations in the high cycle region and under random loading.