Effects Of Multi-enzyme Research Articles

The effects of multi-enzyme and betaine on growth performance, body composition haemato-immunological parameters and expression of growth-related genes in beluga (Huso huso)

The present study investigated the effect of apsozyme and betaine on growth performance, carcass composition, haemato-immunological parameters, and expression of growth hormone (GH) and insulin-like growth factor 1 (IGF1) encoding genes in beluga sturgeon ( Huso huso ). Fish (31.5 ± 0.5 g) were fed with different levels of multi-enzyme (i.e., 0, 250, and 500 mg) and attractant (i.e., 0.5%, 1.0%, and 1.5%). The results obtained from this study indicated that no significant difference was detected in weight gain, specific growth rate (SGR), feed conversion ratio (FCR), and growth-related genes expression with an increase in betaine in experimental treatments ( P > 0.05). However, an increase in apsozyme from 250 mg to 500 mg without betaine resulted in significant elevations in weight gain, SGR, FCR, and growth-related gene expression ( P < 0.05). The highest growth performance (weight gain, SGR, and FCR) and growth-related gene expression were observed in combined treatments (i.e., 500 mg apsozyme +1.0% and 1.5% betaine) ( P < 0.05). No significant difference was detected in carcass composition in different treatments ( P > 0.05). Also, the blood biochemical factors including glucose, and hepatic enzymes, i.e., alkaline phosphatase (ALP), alanine aminotransferase (ALT), and aspartate aminotransferase (AST), were not found significantly different ( P > 0.05), whereas significant increases were observed in protein, albumin, and lysozyme with increased apsozyme in the diet in combined treatments ( P < 0.05). Overall, it is concluded that apsozyme and betaine together could positively affect growth performance parameters, growth-related genes, and haemato-immunological parameters in H. huso . • Combine administration of apsozyme and betaine improved growth performance of beluga. • Growth related genes expression were upregulated in beluga juvenile fed multi enzyme and attractant. • Apsozyme and betaine affected haemato-immunological parameters of beluga.

Effects of multi-enzyme on production performance, egg quality, nutrient digestibility, and excreta noxious gas emission of early phase Hy-line brown hens

The purpose of this study was to investigate the effects of dietary supplementation of non-starch polysaccharide multi-enzyme (NME) in early laying phase of hens on production performance, egg quality, nutrient digestibility, and excreta noxious gas emission. In total, 432 Hy-line brown laying hens at 18 wk of age were used in a 10-wk feeding trail. Hens were randomly assigned to 1 of 3 treatments with 24 replication and 6 hens per replication (1 hen per cage). Dietary treatments were corn-soybean meal-DDGS-based diets supplemented with 0 (based diet, CON), 0.05% (NME1), and 0.1% (NME2) of NME. No significant (P > 0.05) response to increasing NME supplementation was observed for damaged egg rate shown throughout the experiment. Significant (P < 0.05) linear increase was observed for egg production at week 4, 6, and 8; moreover, egg production at week 8 also showed quadratic (P = 0.0344) increase. No significant effects were found on yolk color, eggshell strength, and eggshell thickness during the experiment (P > 0.05) with the increase in NME supplemental levels. Albumin height, haugh unit, and egg color values were linearly (P < 0.05) increased at week 2 and 6 following the increasing NME supplementation, respectively. Additionally, quadratic (P = 0.0013) effect was observed on egg weight at week 6 with the increasing level of NME. Moreover, apparent total tract digestibility of nitrogen and excreta ammonia emission was linearly (P < 0.05) affected increasing NME supplementation. In summary, inclusion of NME containing xylanase, β-glucanase, galactosidase, and galactomannanase activities in corn-soybean meal-DDGS-based diets increased nitrogen digestibility, decreased excreta ammonia emission, and had no negative effects on production performance and egg quality parameters.

Effects of Eco-friendly Multi-enzyme on Diarrhea and Immune Response of weaned Pigs

This study was designed to investigate the effects of multi-enzyme on diarrhea and immune responses of weaned pigs. A total 36 weaned pigs (5.92 ± 0.48 kg BW; 28 d old) were randomly allotted to 2 dietary treatments (3 pigs/pen, 6 replicates/ treatment) in a randomized complete block design. The dietary treatments were a typical diet based on corn and soybean meal (CON) and CON with 0.1% multienzyme (Multi; mixture of β-mannanase, xylanase, α-amylase, protease, β-glucanase, and pectinase). Pigs were fed their respective diets for 6 wk. Frequency of diarrhea, levels of packed cell volume (PCV), white blood cells (WBC), immunoglobulins, cortisol, tumor necrosis factor-α (TNF-α), transforming growth factor- β (TGF-β), and C-reactive protein (CRP) were measured. Multi group tended to decrease (p<0.1) diarrhea frequency than CON group during 2 wk after weaning. Lower values of PCV on d 3 (p<0.05) and d 7 (p<0.1) were found in Multi group compared with CON group. There were no significant differences on WBC number and immunoglobulin (Ig) M and A between Multi and CON groups. However, Multi group tended to increase (p<0.1) Ig G on d 7 than CON group. Moreover, Multi group showed modulated immune responses, indicated by decreased levels of cortisol (p<0.05) on d 7 and 14, TNF-α on d 3 (p<0.05) and d 7 (p<0.10), TGF- β on d 2 (p<0.05) and d 7 (p<0.10), and CRP (p<0.10) on d 3 and 7 after weaning compared with CON group. Consequently, inclusion of multi-enzyme in diets for weaned pigs improved gut health and modulated immune responses of weaned pigs.

Effect of Multienzymes and Absorption Enhancers on Productive Performance, Gut Morphology and Some Blood Biochemical and Hormonal Parameters in Broiler Chicks

The present study was carried out to investigate the effect of polyzyme and lysoforte either alone or in combination on productive performance and some metabolic parameters in broiler chicks. Two hundred and forty, one day old, Cobb chicks were divided into four groups, the first group was fed on a basal ration without any additives and kept as a control, the second group was fed on a basal ration supplemented with polyzyme at a dose of 400 gm/ton ration, the third group was fed on a basal ration to which lysoforte was added at a dose of 250 gm/ton ration and the fourth group was fed on a basal diet to which polyzyme 400 g/ton and lysoforte 250 g/ton were added. Both feed additives either alone or in combination produced a significant improvement in body weight, body weight gain and feed conversion ratio during most of the first seven weeks. All supplemented groups showed no significant changes in the activities of ALP, AST, ALT and levels of creatinine and cholesterol versus control during the whole sampling periods. Polyzyme induced a significant increase in serum triglycerides level at the 4th week of the experiment, while at the 6th and 8th week both polyzyme and lysoforte produced a significant increase in its level versus control. Blood glucose level was significantly increased in lysoforte supplemented groups at the 6th weeks and in polyzyme and lysoforte supplemented groups at the 8th weeks. No significant changes in TSH and T4 levels were recorded during the whole sampling periods. At the 8th week the control group exhibited the lowest level of T3. The length of villi and depth of crypts of small intestine of supplemented groups were significantly higher than those of the control groups. All feed additives did not produce any obvious histopathological changes in the examined organs.

Replacing Soybean Meal (SBM) by Canola Meal (CM) : The Effects of Multi-enzyme and Phytase Supplementation on the Performance of Growing and Laying Quails

In this research, the possibilities of using canola meal (CM) in place of soybean meal (SBM), and also the effects of multi-enzyme and phytase supplementation on the performance of quails were investigated. For this purpose, soybean meal (44% CP), canola meal (37% CP), phytase (produced from Peniophora luci) and multi-enzyme (β-glucanases, pectinases, cellulases and hemicellulases) were used. CM was used supplying 0, 25 and 50% of CP from SBM and each of the phytase and multi-enzyme blends were added to the each level. This study was conducted with 675 day old quails (Coturnix coturnix Japonica) in 9 groups with 3 replicates including 25 birds (mixed sex) per replicate. Nine isocalaric and isonitrogenous diets were prepared. The effects of enzymes and CM levels were studied with a 3×3×3 factorial arrangement for three CM levels (0, 25 and 50%), three treatments (without enzyme, phytase enzyme and multi-enzyme) and three replicates. While the 25% CM level did not affect the liveweight gain 50% CM level decreased the liveweight gain (p<0.05). Multi-enzyme addition to the 50% CM group increased the liveweight gain compared to the other groups (p<0.05). CM levels and enzyme supplementation had no effect on feed consumption, feed conversion ratio, dressing percentage, viability, tibia ash content, Ca and P contents of tibia ash, viscera weight, gizzard weight and length of growth period. While heart weight and liver weight were not affected by CM levels, but they were affected by enzyme supplementation. CM levels and enzyme supplementation did not affect final liveweight, feed consumption, feed conversion ratio, egg yield, egg weight, shell weight and shell index during laying period. The increase in the CM level lightened the colour of the yolk (p<0.05). (Asian-Aust. J. Anim. Sci. 2005. Vol 18, No. 10 : 1457-1463)