Effect Of Microcapsules Research Articles

Impact of Orally Administered Microcapsules on Gastrointestinal Microbial Flora: In-Vitro Investigation Using Computer Controlled Dynamic Human Gastrointestinal Model

Oral administration of artificial cell microcapsules has been proposed for various therapy procedures using biologically active materials. Recently we have designed novel APPPA microcapsules using alginate, poly-L-lysine, pectin, poly-L-lysine and alginate that have shown superior oral delivery features. This article investigates, in-vitro using a computer controlled dynamic gastrointestinal (GI) model, effects of APPPA microcapsules on health of gastrointestinal (GI) microbial flora. The impact of APPPA microcapsules on GI bacterial population, total anaerobes, total aerobes, Escherichia coli, Lactobacillus sp. and Staphylococcus sp. has been analyzed. In addition, the effects of microcapsules on GI microbial extracellular enzymatic activities have been investigated. Result shows the altered activities of microbial flora and enzymes due to the use of APPPA microcapsule. The most disparity is observed in the colon ascendans microbial activities. This study would have significant impact on future microcapsule design. However, further in-vivo studies are required.

Researches Regarding the Use of Coacervates Essential Oils in Seed Treatment in Ecological Crop Production

Following the continuous increase of population, the request for assuring sufficient food is a fundamental objective for all farmers. In these conditions the request for ecological food production has increased as well. Answering this problem, the objective of this research paper is to establish if the microencapsulated essential oils, obtained by us in the experiments at laboratory scale, may be used to protect the wheat seed from attack of pathogens and pests in soil after seeding. The research was performed in laboratory and in experimental field. The laboratory researches was focused on microencapsulation of essential oils and on testing the phytotoxicity effect of microcapsule upon the wheat seeds and as well for test upon the population of agriotes sp, melolontha melolontha larvae stage I, II. The microcapsules were obtained by a process of complex coacervation, and they have a central core formed by an essential oil, covered with a shell made of crosslinked hydrolyzed collagen. In the experimental field was evaluated the intensity of pests attack frequency. The results of phytotoxicity tests show that all the products used in seed treatments don�t have any phytotoxic influence at the used doses; on the contrary we identified a stimulating effect upon the plant growth. The researches regarding the influence upon the melolontha melolontha populations show the products with essential oil from Thymus vulgaris, Satureja hortensis, Ocimum basilicum decreasing the intensity of attack by comparing with control variant without any treatment. By comparing with control treated using imidacloprid, in laboratory, the efficacy of treatment was around 50% from that one. In the experimental field we found the decreasing significantly of aphidae attack frequency. Analyzing the results obtained in this research we can said: `all the seed treatments based on own microencapsulated essential oils, can be used successfully for wheat pest protect in ecological system`.

Effects of microencapsulated monosialoganglioside GM1 on cholinergic neurons

The preparation, physical characterization and effects of microcapsules containing the monosialoganglioside GM1 in an in vivo rat model are described herewith. Several preparations of microcapsules were obtained differing in physical and chemical properties. Human serum albumin (HSA) microcapsules with or without GM1 are spherical in shape, have a consistent particle size (8–10 μm in diameter) and are devoid of large pores. In agreement with our previous work, we now provide further evidence that GM1 can prevent shrinkage and the decrease of choline acetyltransferase activity in the nucleus basalis magnocellularis (NBM) of the rat following a unilateral cortical lesion. In the present study we examined the effect of microencapsulated GM1 in this in vivo rat model. Local application of HSA-microencapsulated GM1 (in doses comparable to those obtained by i.c.v. administration) onto the surface of the lesioned cortex prevents both the biochemical and morphological degenerative changes in the NBM of rats with unilateral devascularizing cortical lesions. The results from these studies show that microencapsulated GM1 can be applied successfully and a prolonged controlled release of this drug obtained, thus avoiding surgical implantation of a cannula.