Effect Of Methionine Deficiency Research Articles

Effects of methionine deficiency on B7H3-DAP12-CAR-T cells in the treatment of lung squamous cell carcinoma

Lung squamous cell carcinoma (LUSC) is a subtype of lung cancer for which precision therapy is lacking. Chimeric antigen receptor T-cells (CAR-T) have the potential to eliminate cancer cells by targeting specific antigens. However, the tumor microenvironment (TME), characterized by abnormal metabolism could inhibit CAR-T function. Therefore, the aim of this study was to improve CAR-T efficacy in solid TME by investigating the effects of amino acid metabolism. We found that B7H3 was highly expressed in LUSC and developed DAP12-CAR-T targeting B7H3 based on our previous findings. When co-cultured with B7H3-overexpressing LUSC cells, B7H3-DAP12-CAR-T showed significant cell killing effects and released cytokines including IFN-γ and IL-2. However, LUSC cells consumed methionine (Met) in a competitive manner to induce a Met deficiency. CAR-T showed suppressed cell killing capacity, reduced cytokine release and less central memory T phenotype in medium with lower Met, while the exhaustion markers were up-regulated. Furthermore, the gene NKG7, responsible for T cell cytotoxicity, was downregulated in CAR-T cells at low Met concentration due to a decrease in m5C modification. NKG7 overexpression could partially restore the cytotoxicity of CAR-T in low Met. In addition, the anti-tumor efficacy of CAR-T was significantly enhanced when co-cultured with SLC7A5 knockdown LUSC cells at low Met concentration. In conclusion, B7H3 is a prospective target for LUSC, and B7H3-DAP12-CAR-T cells are promising for LUSC treatment. Maintaining Met levels in CAR-T may help overcome TME suppression and improve its clinical application potential.

Effects of Methionine Deficiency on Nutrient Composition, No, Nos Activity and Mrna Expression in Nf-κb Signal Pathway of the Liver and Kidney in Broiler

Effects of Methionine Deficiency on Nutrient Composition, No, Nos Activity and Mrna Expression in Nf-κb Signal Pathway of the Liver and Kidney in Broiler

Effect of Methionine Deficiency on the Growth Performance, Serum Amino Acids Concentrations, Gut Microbiota and Subsequent Laying Performance of Layer Chicks.

This study was conducted to investigate the effect of methionine (Met) deficiency in the rearing period on the growth performance, amino acids metabolism, intestinal development and gut microbiome of egg-laying chicks and the continuous effects on the performance, egg quality, and serum amino acids metabolism of the subsequent development process. Three hundred sixty one-day-old chicks were randomly divided into two groups and fed on a basal diet (NC group, Met 0.46%) and Met deficiency diet (Met- group, Met 0.27%). Each group included six replicates with 30 chicks per replicate. The trial lasted 6 weeks (0–6 weeks), both groups were fed the same basal diet which met the needs of Met during the observation period (7–24 weeks). Results showed that Met deficiency significantly decreased (P < 0.05) body weight (BW), average daily weight gain (ADG), average daily feed intake (ADFI) and tibia length (TL) compared to the NC group during the trial period (0–6 weeks). Also, Met deficiency dramatically increased (P < 0.05) feed conversion ratio (FCR) during the trial and observation period (7–24 weeks). In addition, during the observation period, the BW and ADG were decreased (P < 0.05) in the Met- group. Moreover, Met- group decreased (P < 0.05) villi height and villi height/crypt depth ratio in jejunum at 6th weeks. In addition, the concentrations of serum main free amino acids (FAA) in the Met- group were significantly increased (P < 0.05) at 6th weeks, while were decreased at 16th weeks. Based on the α-diversity and PCoA analysis in β-diversity, there were no significant differences in the cecal microbial composition between NC and Met- groups. However, the LEfSe analysis revealed that differential genera were enriched in the NC or Met- groups. The Haugh unit, shell thickness and egg production in the Met- group were significantly lower (P < 0.05) than in the NC group. In conclusion, these results revealed that dietary supplementation of appropriate Met could substantially improve the growth performance, host amino acid metabolism and intestinal development and continuously improve the laying performance and thus boost the health of growing hens.

Effects of methionine deficiency on the number of IgA+ B cells and immunoglobulins in ileum of broiler chicken

Effects of methionine (Met) deficiency on IgA+ B cells and immunoglobulins (Igs) in the ileum of Cobb broiler were observed by the methods of immunohistochemistry and enzyme-linked immunosorbent assay (ELISA) ranging from 1 to 42 days of age. Results showed a significant decrease of the number of IgA+ B cells and the amount of Igs in Met deficient group in comparison with control group. In conclusion, dietary Met deficiency exerts significant effect on the humoral immune function of the intestinal mucosa. This study would provide new experimental information for future studies in the effects of Met on the intestinal functions of human or other animals.

Effect of low methionine formula on levels of IL-1β Serum and IL-1β gene expression in knee joint cartilage tissues of normal rabbits and ACl induction OA models

Methionine deficiency is an environmental factor can degrade the quality of the bone, cartilage and modulate chondrocytes to increase protease secretion and change gene expression. The aim of the study was to determine the effect of methionine deficiency on IL-1β serum, expression of IL-1β in cartilage tissue of knee joints of New Zealand rabbit's (Oryctologus cuniculus). The experimental animals were divided into 6 treatment groups: normal group with the addition of DL-methionine 0.25%, normal group with the addition of DL-methionine 0.15%, normal group with the addition of DL-methionine 0.00%, ACL group with the addition of DL-methionine 0.25%, ACL group with the addition of DL-methionine 0.15%, ACL group with an addition of 0.0%. Examination of IL-1β serum by ELISA with RayBio Rabbit commercial kit. Examination of IL-1β expression immunohistochemically using IL-1β anti-rabbit primary antibody with a Santa Cruz commercial kit (Sc7884). The results were analysed using one way ANOVA, followed by LSD. The results indicate that methionine deficiency (DL-methionine 0.0%) is able to increase IL-1β serum. The expression of IL-1β in knee joint cartilage tissue appears to be increased significantly through the metabolic effects or interactions with biomechanical changes. Methionine deficiency has the same ability in normal and pathological conditions, has a tendency to increase IL-1β serum and IL-1β gene expression in joint cartilage tissue.

Effects of Methionine Deficiency and Ethanol Ingestion on Acetaminophen Metabolism in Mice

The interaction of the effect of dietary methionine on the avallability of hepatic glutathione (GSH) and the effect of chronic ethanol (EtOH) consumption on the activity of the hepatic oxidation system was studied in relation to acetaminophen (ACAP) metabolism in mice. Adult male Swiss-Webster mice were pair-fed for 4 wk an EtOH-containing liquid diet that provided 50 or 100% of the methionine requirement in a 2 × 2 factorial design. Hepatic microsomal protein, relative liver weight and microsomal aniline hydroxylase activity were higher in EtOH-fed groups than in non-EtOH-fed groups. After an ACAP dose of 300 mg/kg body wt i.p., serum inorganic sulfate, endogenous hepatic methionine and GSH concentrations were lower, whereas uridine diphosphoglucuronosyltransferase activity was not changed compared to controls. GSH levels were lowered to a greater extent in the methionine-deficient groups than in methionine-sufficient groups. Incorporation of [35S]methionine into hepatic proteins was lower in all treatment groups after ACAP administration than in controls. The distribution of ACAP into the urinary sulfate conjugates was lower in methionine-deficient than in methionine-sufficient groups, and the percentage of sulfate and mercapturic acid conjugates formed as determined by high-performance liquid chromatographic analysis was higher in mice fed EtOH than in controls. Methionine deficiency compromises the normal pathways of ACAP disposition in the mouse, and chronic EtOH ingestion may potentiate this effect by increasing the amount of activated ACAP formed.

The effects of methionine deficiency and egg production on feather loss in caged layers.

Feather loss was scored on the neck and crop area of 402 individually-caged hens, 61 weeks old and receiving either methionine-deficient or adequate diets. Both feather loss and egg production were greater for the hens receiving adequate diets. Within treatments there was a close relationship between egg output and feather loss. The findings are consistent with feather loss being caused by abrasion against the cage floor or walls during pre-laying behaviour.

The Effect of Methionine Deficiency on Body Weight, Food and Energy Utilization in the Chick

The effect of dietary methionine deficiency was studied in broiler chicks. Graded levels of methionine: 0.25, 0.32, 0.39 and 0.46%, in semipurified, soybean meal-corn starch diets were used as treatments. Food intake, body weights and body components were determined. Results show that a moderate methionine deficiency (0.32 and 0.39%) had no effect on growth and energy metabolism expressed as metabolizable energy, productive energy, heat production and tissue gains. Increased food intake in these two groups was reflected not as increased weight gain, but as greater quantities of tissue fat. The experimental group with severe methionine deficiency (0.25%) showed depressed body weight gain, food intake and efficiency of food utilization, with some increase in heat production. Therefore, in general, two types of nutritional responses occurred in broilers, based upon the severity of the methionine deficiency.

The effect of chronic methionine deficiency on the tissue distribution of 60Co-cyanacobalamin in the rat

The effect of methionine deficiency on the metabolic fate of cyanocobalamin in rats was studied. No significant differences in the absorption and excretion of orally administered 60Co-labeled cyanocobalamin were noted between methionine-deficient and methionine-supplemented groups. The retention of injected 60Co-labeled cyanocobalamin by the kidney, liver, spleen and heart of methionine-deficient rats was higher than that of methionine-supplemented rats. These results suggest that there may be an increase in the utilization of vitamin B 12 to ameliorate methionine deficiency by sparing methyl group and/or by increasing methionine synthesis.

Effect of Methionine Deficiency on the Utilization of Energy by the Chick

Effect of Methionine Deficiency on the Utilization of Energy by the Chick

The Effect of Methionine Deficiency on Nitrogen Absorption from the Intestinal Tract of Chickens

The Effect of Methionine Deficiency on Nitrogen Absorption from the Intestinal Tract of Chickens

Sex difference in induction of periportal fatty liver by methionine deficiency in the rat.

Summary1. Force-feeding of purified choline-containing diets devoid of methionine for 3 to 6 days induces fatty liver in adult female rats, but not in adult male rats. Excess lipid accumulates in the periportal region of liver lobule. 2. Force-feeding of threonine-devoid diet for 3 days to adult rats also induces periportal fatty liver but without a sex difference. 3. Occurrence of the same sex difference and the same lobular distribution of fat in the liver by force-feeding methionine devoid diets and by injection of ethionine lends further support to the thesis that ethionine exerts some of its effects through interfering with metabolic role of methionine. 4. Since choline does not protect liver against the effects of methionine deficiency, it appears that methionine must play some role in regulation of lipid content of liver aside from its contribution of methyl groups for synthesis of choline. It is suggested that this role of methionine is related in some as yet unknown manner with the action of andr...

Effect of Methionine Deficiency upon Enzyme Activity in the Rat.

SummaryIt has been observed that a methionine deficiency in the rat reduces liver succinic dehydrogenase activity slightly, completely reduces liver xanthine oxidase activity, and has practically no effect upon endogenous respiration of liver tissue in vitro.