Effects Of Magnesium Chloride Research Articles

Novel insights into the effect of magnesium chloride or combined with glucono-δ-lactone on the quality of tofu

Novel insights into the effect of magnesium chloride or combined with glucono-δ-lactone on the quality of tofu

Influence of magnesium chloride on the electrodeposition of zinc from the 1,3-dimethyl-2-imidazolidinone/ZnCl2 system

The effect of magnesium chloride (MgCl2) on the electrodeposition of zinc in 1,3-dimethyl-2-imidazolidinone (DMI)/ZnCl2 solvate ionic liquids was investigated. Cyclic voltammetry and Chronoamperometry are employed to study the electrochemical behavior of Zn. The crystal structure, microscopic morphology, and roughness of Zn coatings were measured by X-ray diffraction (XRD), scanning electron microscopy (SEM), and Non-contact Optical Surface Profiler (OSP). The results reveal that adding MgCl2 is favorable to the electrodeposition of Zn and leads to the formation of a new zinc-containing species in the electrolyte, which was confirmed by Raman spectra. Importantly, this addition does not affect the nucleation mechanism of zinc. Furthermore, the zinc grows preferentially on the (101) plane and a more smooth and uniform zinc coating was obtained. Corrosion resistance testing is conducted in a 3.5 wt% NaCl solution using the Tafel method. Coatings obtained at 4 mA/cm2 and 343 K with 0.1 mol/L MgCl2 addition exhibit lower corrosion current density of 38.10 μA/cm2 compared to zinc coatings obtained without MgCl2.

Effect of Magnesium Chloride in Supporting Electrolyte for Enhancing Sensitive and Selective Electrochemical Sensor: An Approach for Anti-Rheumatic Sulfasalazine Detection

In this work, an electroanalytical evaluation for voltammetric sensing of the anti-rheumatic sulfasalazine (SSZ) at an unmodified screen-printed graphene electrode (SPGE) is demonstrated. By using the differential pulse (DPV) technique, the SSZ produced a well-defined peak of around −0.3 V (vs Ag AgCl−1) in Britton-Robinson (BR) buffer pH 4. Supporting electrolytes, pH, and salts all significantly impact SSZ reduction. Therefore, their impact on the working solutions was assessed. We discovered that using a mixture of Britton–Robinson (BR) buffer with pH 4 and MgCl2 as a supporting electrolyte can enhance SSZ sensitivity by approximately 1.7 times while simultaneously increasing detection selectivity. Under optimal conditions, the proposed assay demonstrated the ultrasensitive determination of SSZ with a broad linear detection range from 0.01 to 100 μM and a low detection limit of 4.7 nM (S/N = 3). To demonstrate the impact of the proposed method, the sensor has been successfully applied for the quantitative determination of SSZ in pharmaceutical, urine, and artificial serum sample. Therefore, this approach could offer simplicity, and rapidity, and serve as an alternative to the SSZ detection in practical applications.

The Effect of Magnesium Chloride on the Macroscopic and MI-Croscopic Properties of Phosphate Cement-Based Materials

Phosphate cement-based materials are fast-hardening cement materials, which have been applied to the rapid repair of concrete structures. However, the excessive setting rate could lead to initial cracks in the cement-based matrix. Therefore, a proper retarder is required to reduce the setting rate, thus improving the strength of structures. In this study, a magnesium chloride retarder was selected, and its influence on the setting time, slump flow, and the mechanical strengths (flexural strength, compressive strength, and bond strength) of phosphate cement paste curing for 3 h~28 d was investigated. Scanning electron microscopy, X-ray diffraction, and thermal analysis were used to analyze the mechanism of the properties of phosphate cement paste. Results showed that the setting time increased exponentially with the mass ratio of magnesium chloride by the total mass of magnesium oxide. Meanwhile, the slump flow increased linearly with the increasing dosage of magnesium chloride, and the drying shrinkage rate exhibited a quadratic function with the curing age. The addition of magnesium chloride decreased the mechanical strengths of phosphate cement paste at earlier curing age (lower than 3 d) and effectively improved the mechanical strengths at a later curing age (equal to or higher than 3 d). Moreover, magnesium chloride could also decrease the drying shrinkage rate. It can be obtained from the microcosmic researching results that magnesium chloride can inhibit the hydration of phosphate cement and reduce cracks induced by drying shrinkage at later curing age (higher than 3 d).

Experimental Study on Hydrothermal Carbonization of Lignocellulosic Biomass with Magnesium Chloride for Solid Fuel Production

The effect of magnesium chloride as an additive of hydrothermal carbonization (HTC) of lignocellulosic biomass (Pinus radiata sawdust) was studied. The HTC tests were carried out at fixed conditions of temperature and residence time of 220 °C and 1 h, respectively, and varying the dose of magnesium chloride in the range 0.0–1.0 g MgCl2/g biomass. The carbonized product (hydrochar) was tested in order to determine its calorific value (HHV) while using PARR 6100 calorimeter, mass yield by gravimetry, elemental analysis using a LECO TruSpec elemental analyzer, volatile matter content, and ash content were obtained by standardized procedures using suitable ovens for it. The results show that using a dose of 0.75 g MgCl2/g biomass results in an impact on the mass yield that was almost equal to change operating conditions from 220 to 270 °C and from 0.5 to 1 h, without additive. Likewise, the calorific value increases by 33% for this additive dose, resulting in an energy yield of 68%, thus generating a solid fuel of prominent characteristics.

Dissolution of Mg Fertilizer and Its Availability in Cassava in Tropical Upland Soils

ABSTRACTEffect of magnesium chloride (MgCl2) and dolomite on magnesium (Mg) availability cassava was observed by a pot experiment under greenhouse condition in three Paleustults. Their water solubilities varied greatly, with increased Mg availability in the soils. This resulted in cassava taking up more Mg and producing 23.3–45.2% and 18.8–26.2% greater aboveground biomass over the control when respective MgCl2 and dolomite were added. Without Mg fertilization, Mg contents, mainly in a component of minerals, were low and constant throughout 120 days of the study, indicating that initial Mg content in these soils was insufficient for cassava growth. Magnesium became available at 1-month after dolomite was applied. Magnesium in MgCl2-added soil was predominated by available Mg; moreover, MgCl2 evidently boosted the soluble Mg portion within 15 days after applying. After that, the proportion decreased similarly to that of the other treatments. It indicated that dolomite was a better Mg source in supplying Mg for a longer period than MgCl2. In addition, the Mg contents in the soils gradually increased early and then sharply decreased at 30 days, and then the contents were constant until the end of the study. The result clearly signified that cassava absorbed Mg rapidly within 1.5 months after planting. Among soil extractants tested in the study, 0.05 M HCl was the best available Mg extraction suitable for cassava crop, whereas 1 M NH4OAc, the routine soil test in Thailand, was the least suited extractant.

The modification mechanism and the effect of magnesium chloride on poly(vinyl alcohol) films.

The modification mechanism and the effect of MgCl2 on poly(vinyl alcohol) and the effect of water in the system were studied in this paper. The effect of MgCl2 on different fully dried PVA was investigated by Fourier transform infrared spectroscopy, X-ray diffraction, differential scanning calorimeter, thermogravimetric analysis, water content test, dynamic thermomechanical analysis and tensile test, respectively. The results showed that MgCl2 was an effective strengthening agent, which could crosslink with PVA and then effectively decrease the crystallinity of PVA. With the addition of MgCl2, the strength and rigidity of PVA increased and the melting temperature decreased. The effect of MgCl2 on thermal stability of PVA was related to the alcoholysis degree of PVA. The tensile strength of PVA films modified with MgCl2 were all over 120 MPa. Moreover, the effect of MgCl2 on the dynamic thermomechanical properties and tensile properties of PVA with various content of water was studied. Water could interact with MgCl2 to destroy the crosslink between MgCl2 and PVA. In the presence of water, PVA modified with MgCl2 became soft, with lower tensile strength and higher elongation at break.

Chitosan-based thermosensitive hydrogel for nasal delivery of exenatide: Effect of magnesium chloride

The aim of this research was to evaluate the potential of two chitosan (CS)-based hydrogel systems for nasal delivery of exenatide (EXT) in rats. Both of the EXT-loaded CS/glycerophosphate (GP)/CaCl2 (EXT/CS/GP/CaCl2) and EXT/CS/GP/MgCl2 hydrogel systems had similar in vitro release profiles. However, a difference in metal salt surprisingly resulted in multifaceted differences between the two hydrogel systems, such as EXT stability, gelation time, transepithelial transport, biodistribution and pharmacokinetics. The gelation time of the EXT/CS/GP/MgCl2 hydrogel (more than 110 min) at 37 °C was much longer than that of the EXT/CS/GP/CaCl2 hydrogel (8.0–20.4 min). Transepithelial transport analysis showed that the CS/GP/MgCl2 hydrogel enhanced EXT transport across the Calu-3 cell monolayers more than the CS/GP/CaCl2 hydrogel (P < 0.05). After nasal administration in the rats, the EXT/CS/GP/MgCl2 hydrogel increased the distribution of EXT in the targeting organs and the relative bioavailability of EXT in the rats more than the EXT/CS/GP/CaCl2 hydrogel. Moreover, both EXT/CS/GP/salt hydrogel formulation treatments in high-fat-fed rats significantly decreased food intake and body weight relative to the EXT solution within ten days (P < 0.05). The aforementioned results suggest that the EXT/CS/GP/MgCl2 hydrogel formulation is more suitable to be used as a nasally delivered EXT formulation for the treatment of weight loss.

Effects of magnesium chloride on the anaerobic digestion and the implication on forward osmosis membrane bioreactor for sludge anaerobic digestion

This work elucidates the effects of model reversed salt MgCl2 on methane production in an anaerobic digestion bioreactor treating waste sludge. Along with MgCl2 concentration being raised stepwise, the methane production was only slightly less than in the control when MgCl2 was 20 g/L and under, and then suddenly reduced to only about 10 mL/(L·d) at a MgCl2 concentration of 30 g/L, and finally stopped when the MgCl2 concentration reached 50 g/L. However, the total relative abundance of methanogens Methanomicrobia and Methanobacteria still accounted for 84.97% of the archaeal community when MgCl2 was 50 g/L. The high correlation between live/dead cell ratio and methane production suggests that the live/dead cell ratio instead of the inhibition of methanogen might be the major cause for the halt of methane production at a magnesium chloride concentration of 50 g/L.

Magnesium chloride and sulfate attacks on gravel-sand-cement-inorganic binder mixture

Investigation on the degradation of improved ground is crucial for predicting its life span and performance under unfavorable conditions. In this study, effects of magnesium chloride (MgCl2) and magnesium sulfate (MgSO4) attacks on gravel-sand-cement-inorganic binder mixtures were investigated. Electrical resistivity, free-free resonant column (FFRC) and uniaxial compression tests were performed at different exposure periods, and results showed that saline solutions exert considerable effects on the geophysical parameters of the mixture. As the samples were exposed to chemical attacks, the electrical resistivity and damping ratio increased, while the longitudinal wave velocity and UCS decreased. Especially, the results at 28 days showed that mixtures without inorganic binder lost approximately 40% of its initial strength in MgCl2 and 12% in MgSO4, while samples with inorganic binder (IBS) only lost about 30% and 8% in MgCl2 and MgSO4 respectively. Thus, it is expected that increasing the inorganic binder content in the mixture can enhance the performance of the grout and reduce strength degradation induced by chemical attacks.

Effects of magnesium chloride on invitro cholinesterase and ATPase poisoning by organophosphate (chlorpyrifos).

The present study investigated possible benefits of magnesium ion (as MgCl2) in organophosphorus poisoning targeting its ability to interact with substrates and membrane enzymes. Blood samples collected from volunteered healthy adult by venepuncture into anticoagulant test tubes containing EDTA were separated into plasma and red blood cell and divided into three groups namely: normal, pesticide only (0.25‐2.0 mmol/L chlorpyrifos) and pesticide (0.25‐2.0 mmol/L chlorpyrifos) + 0.1 mol/L MgCl2. Acetylcholinesterase, Na+/K+ ATPase and Ca2+ ATPase activities were evaluated. Results showed that Chlorpyrifos significantly (P < .05) reduced the levels of cholinesterase both in plasma and on red blood cells. Red blood cells Na+/K+ ATPase and Ca2+ ATPase were also significantly (P < .05) reduced by chlorpyrifos while MgCl2 counteracted effects of chlorpyrifos with significant (P < .05) increase in the levels of cholinesterase, Na+/K+ ATPase and Ca2+ ATPase. We concluded that MgCl2 neutralized effects of chlorpyrifos by promoting normal ATPase activities and inhibiting release of acetylcholine from cell.

Effects of magnesium chloride on rocuronium-induced neuromuscular blockade and sugammadex reversal in an isolated rat phrenic nerve-hemidiaphragm preparation: An in-vitro study.

Magnesium potentiates the effects of nondepolarising muscle relaxants. However, few studies have used magnesium chloride (MgCl2). Sugammadex reverses neuromuscular block by steroidal nondepolarising muscle relaxants. To assess the effects of MgCl2 on rocuronium-induced neuromuscular blockade and its reversal by sugammadex. In-vitro experimental study. Animal laboratory, Asan Medical Center, Seoul, South Korea, from 20 March 2016 to 3 April 2016. Forty male Sprague Dawley rats. Left phrenic nerve-hemidiaphragms from 40 Sprague Dawley rats were allocated randomly to four groups (1, 2, 3 and 4 mmol l MgCl2 group, n = 10 each). Rocuronium was administered cumulatively until the first twitch of train-of-four (TOF) disappeared completely. Then, equimolar sugammadex was administered. The effective concentration (EC) of rocuronium was obtained in each group. After administering sugammadex, recovery of the first twitch height and the TOF ratio were measured for 30 min. EC50, EC90 and EC95 significantly decreased as the concentration of MgCl2 increased (all P ≤ 0.001), except the comparison between the 3 and 4 mmol l MgCl2 groups. After administration of sugammadex, the maximal TOF ratio (%) was lower in the 4 mmol l MgCl2 group than the 1 mmol l MgCl2 group [median 91.7 interquartile range (83.4 to 95.8) vs. 98.3 interquartile range (92.2 to 103.4), P = 0.049]. The mean time (s) from sugammadex injection to achieving maximal first twitch was significantly prolonged in the 4 mmol l MgCl2 group vs. the 1 mmol l MgCl2 and 2 mmol l MgCl2 groups [1483.9 (± 237.0) vs. 1039.0 (± 351.8) and 926.0 (± 278.1), P = 0.022 and 0.002, respectively]. Increases in MgCl2 concentration reduce the ECs of rocuronium. In addition, administering sugammadex equimolar to the administered rocuronium shows limited efficacy as MgCl2 concentration is increased. The in-vitro study was not registered in a database.

Effect of magnesium chloride on fractionation and enzymatic digestibility of cattails

ABSTRACTThe effect of magnesium chloride (a Lewis acid) on the fractionation of aquatic plant cattail was studied using a Dionex accelerated solvent extractor varying salt concentration (0–0.4 M), treatment temperature (140–180°C), and residence time (5–15 min). MgCl2 treatment enhanced xylan degradation and delignification, and up to 33.8% of total xylan was directly hydrolyzed to the xylose monomer. By removing xylan and lignin, the MgCl2 treatment effectively increased enzymatic digestibility of cattail cellulose and xylan. When cattails were treated with the 0.4 M MgCl2 at 180°C for 15 min and subsequently hydrolyzed for 48 h with a cellulase loading of 15 FPU/g glucan, the highest glucose yield of 61.7% and the highest total xylose yield of 90.6% were reached, respectively. Application of Lewis acids as a novel biomass fractionation approach will require lower xylanase loading for hydrolysis, and have lower corrosion to equipment compared to other acid pretreatment processes.

Effect of Prewetting Brines on the Ice-Melting Rate of Salt at Very Cold Temperatures

At temperatures close to its eutectic, sodium chloride (NaCl) is shown to have substantial ice-melting capacity, but the ice-melting rate is very slow. The effects of magnesium chloride (MgCl2), NaCl, and mixed MgCl2-NaCl brines on the ice-melting rate of prewetted NaCl rock salt at temperatures close to the NaCl eutectic were measured. The solid salt component of a 50:50 mixture of salt and 26.0% MgCl2 brine melted ice 7.1 times faster than dry salt after 3 h, 3.5 times faster after 7 h, and 1.7 times faster after 24 h at an average temperature of −4.48F (−20.2°C). The solid salt component of a 50:50 mixture of salt and 23.3% NaCl brine melted ice 2.9 times faster than dry salt after 3 h, 2.0 times faster after 7 h, and 1.6 times faster after 24 h at the same temperatures. Ice-melting rates with mixed MgCl2-NaCl prewetting brines were intermediate between those of the straight NaCl and MgCl2 prewetting brines. Above a ratio of prewetting brine-to-salt high enough so that the ice was completely covered with brine, there was no significant increase in the ice-melting rate with increased prewetting brine application rate. Preliminary evidence suggests that a contribution to the rate enhancement caused by MgCl2 may be enhanced diffusion of NaCl from the dissolving salt crystals in the MgCl2 brines.

Effect of Magnesium Chloride–Induced Salinity on Carbon Dioxide Evolution and Nitrogen Mineralization in a Silty Clay Loam Soil

A laboratory incubation experiment was conducted to evaluate the effect of magnesium chloride–induced salinity on carbon dioxide (CO2) evolution and nitrogen (N) mineralization in a silty loam nonsaline alkaline soil. Magnesium chloride (MgCl2) salinity was induced at 0, 4, 8, 12, 16, 20, 30, and 40.0 dS m−1 and measured CO2 evolution and N mineralization during 30 days of incubation. Both CO2 evolution and N mineralization decreased significantly with increasing salinity. The cumulative CO2 evolution decreased from 235 mg kg−1 soil at electrical conductivity (EC) 0.65 dS m−1 to 11.9 mg kg−1 soil at 40 dS m−1 during 30 days of incubation. Similarly, N mineralization decreased from 185.4 mg kg−1 at EC 0.65 dS m−1 to 34.45 mg kg−1 at EC 40.0 dS m−1 during the same period. These results suggested that increasing magnesium chloride salinity from 4 dS m−1 adversely affect microbial activity in terms of carbon dioxide evolution and N mineralization.

Effect of Magnesium Chloride (MgCl 2 ) on Ordinary Portland Cement Concrete

This paper reports the effect of Magnesium chloride ( 2 ) on Ordinary Portland Cement (OPC) concrete. The OPC concrete was produced with MgCl 2 dosage of 0.2, 0.5, 1.0, 1.5 and 2.0 g/lit and deionised water was used in concrete mix (M20&M50). In addition to this, control specimens were prepared with deionised water (without MgCl 2 ) for comparison. The compressive and tensile strengths were evaluated for 28 and 90 days. The results show that, as the MgCl 2 concentration increases, the compression and tensile strengths decrease. The X-ray diffraction analysis for concrete has been carried out at 1.5 g/lit concentration.

Effects of magnesium chloride on the contractile response of uterus to several agonists in Ca-free solution.

The effects of MgCl2 on the oestrogen-dominated rat uterus have been examined. Tissues were preincubated in a Ca2+- and Mg2+-free medium containing 3 mM EDTA. Most experiments were subsequently performed in a similar medium containing either no EDTA or EDTA (1 mM). When MgCl2 was added cumulatively (1-32 mM) no contractile responses were obtained in Ca,Mg-free medium or in Ca,Mg-free high K+ solution. When 2 mM CaCl2 was added, a sustained contraction was obtained. Subsequent addition of cumulative concentrations of MgCl2 caused concentration-dependent relaxation. Oxytocin, 2 microM, produced a small and sustained contraction in a Ca,Mg-free medium. Addition of MgCl2, 2 mM, increased this contraction. In a Ca,Mg-free medium vanadate (8 X 10(-5)M) did not evoke spasm of uterine smooth muscle. After addition of MgCl2 in cumulative amounts (1-32 mM) in the presence of vanadate, a concentration-dependent contraction was obtained. The present work shows that in Ca-free solution, maintained contractions induced by oxytocin and vanadate are augmented by Mg2+.

Effect of magnesium chloride on psychomotor activity, emotional status, and acute behavioural responses to clonidine, d-amphetamine, arecoline, nicotine, apomorphine, and L-5-hydroxytryptophan

BackgroundThe beneficial effects of magnesium (Mg) salts on central manifestations of Mg deficiency are well known. Mg replacement therapy can be effective to prevent some of the serious depression-like and anxiety-related behaviour sequelae of Mg deficiency. However, few experimental studies have been undertaken on Mg-deficiency-induced behavioural changes. Even fewer studies have been carried out on acute behavioural responses to clonidine, D-amphetamine, arecoline, nicotine, apomorphine, and L-5-hydroxytryptophan (HTP), which might characterize possible neuromediator changes in Mg deficiency. The effects of correcting Mg deficiency by magnesium chloride (MgCl2 · 6H2O) and the combination of this salt with vitamin B6, on the behavioural manifestations of Mg deficiency have never been described as well.ObjectiveThe aims of this study were: to estimate effect of MgCl2 · 6H2O alone and in combination with vitamin B6 on acute behavioural responses to agonists or blockers of the main neurotransmitter systems in CNS, psychomotor activity and emotional status of rats fed with Mg-deficient diet for 49 days. In our study open field test has shown that in Mg-deficient rats locomotor activity and vertical activity, number of visiting and residence time in central squares were decreased significantly. In the elevated plus maze test, the number of visiting open arms and residence time of rats were significantly less as compared with the control group. In the forced swimming test, time immobile was significantly increased by 44.29% and time of swimming was decreased by 52.79% compared to control.ResultsIn our study Mg-deficient rats were more sensitive to d-amphetamine-induced motor stereotypes. Mg deficiency antagonized 5-hydroxytryptophan-induced head-twitch response and arecoline-induced tremor. Supplement of MgCl2 · 6H2O with vitamin B6 administered to a Mg-deficient rat increased the Mg level in plasma and erythrocytes. Furthermore, this increase was in relation to vitamin B6 given to the animal. Mg supplementation alone and in combination with pyridoxine normalized acute behavioural responses to d-amphetamine, 5-hydroxytryptophan, and arecoline in Mg deficient rats with a return to pre-deficient levels observed in the Mg sufficient group.DiscussionCombination of Mg salts and pyridoxine hydrochloride can be effective at treating some behavior form of primary Mg deficiency.

Effect of magnesium chloride (2:1 electrolyte) on the aqueous solution behavior of some saccharides over the temperature range of 288.15–318.15 K: a volumetric approach

Infinite-dilution standard partial molar volumes, V 2 0 , for various mono-, di-, and trisaccharides, and their derivatives (methyl glycosides) at molalities ranging from 0.04 to 0.12 mol kg −1 in aqueous solutions of magnesium chloride of 0.5, 1.0, 2.0, and 3.0 mol kg −1, have been evaluated over a range of temperatures from 288.15 to 318.15 K by density measurements employing a vibrating-tube densimeter. These data have been utilized to determine the corresponding standard partial molar volumes of transfer, Δ t V 2 0 , of saccharides and methyl glycosides from water to aqueous magnesium chloride solutions. The Δ t V 2 0 values have been found to be positive, and their magnitudes increase with an increasing concentration of magnesium chloride in all cases. Partial molar expansion coefficients, ( ∂ V 2 0 / ∂ T ) P and second derivatives thereof, ( ∂ 2 V 2 0 / ∂ T 2 ) P have been estimated. The magnitude of V 2 0 values increases with an increase in temperature, indicating that hydration effects in solutions are strongly sensitive to temperature. Pair and higher order volumetric interaction coefficients ( V AB, V ABB) have also been obtained from Δ t V 2 0 values by using the McMillan–Mayer theory. The various parameters have been discussed in terms of the solute (saccharide or methyl glycoside)–co-solute (magnesium chloride) interactions and are thus used to understand the mixing effects due to these interactions. These results have been compared with those earlier reported in the presence of electrolytes. An attempt is made to interpret the volumetric properties data in terms of the stereochemistry of the solutes.

Biochromatographic framework for analyzing magnesium chloride salt dependence on nor-NOHA binding to arginase enzyme

Our group demonstrated recently that arginase I inhibition reduces endothelial dysfunction and blood pressure rising in spontaneously hypertensive rats [C. Demougeot, A. Prigent-Tessier, C. Marie, A. Berthelot, J. Hypertens. 23 (2005) 971; C. Demougeot, A. Prigent-Tessier, T. Bagnost, C. Andre, Y. Guillaume, M. Bouhaddi, C. Marie, A. Berthelot, Life Sci. 80 (2007) 1128]. This discovery opens interesting perspectives in the development of new drugs against hypertension. As well, in a previous paper [T. Bagnost, Y.C. Guillaume, M. Thomassin, J.F. Robert, A. Berthelot, A. Xicluna, C. Andre, J. Chromatogr. B: Analyt. Technol. Biomed. Life Sci. 856 (2007) 113], a novel biochromatographic column was developed in our laboratory for studying the binding of N ω-hydroxy-nor- l-arginine (nor-NOHA), an arginase inhibitor, with this enzyme. In this manuscript, using this novel biochromatographic concept, the effect of magnesium chloride on the nor-NOHA/arginase binding was analyzed for the first time. This study demonstrated that the salt ions interacted with arginase and played a great role in the nor-NOHA/arginase association. For a salt concentration ( x) in the medium less than 3 mM, the nor-NOHA/arginase binding decreased with x due to a decrease of the charge–charge interactions between nor-NOHA and its arginase binding site. Above 3 mM of salt in the medium, the affinity of nor-NOHA to arginase increased slightly with x because the net number of ions ( n) (Mg 2+ or Cl −) released or bound upon complex formation is low. As well, it was clearly demonstrated, that above 3 mM the n value depend on the salt concentration in the bulk solvent and was approximately nil for x = 12 mM. This dependence was due to a gradual and conformational change of the arginase enzyme which around 12 mM adopted a less flexible structure; its binding site was thus less accessible to nor-NOHA and nor-NOHA–arginase association decreased slightly.