Effect Of Low-intensity Laser Radiation Research Articles

The effect of low-intensity laser radiation on the sensitivity of Staphylococcus aureus to some halogen-containing azaheterocycles

The rapid rate of acquisition and spread of resistance to antibiotics by microorganisms leads to the search and investigation of new ways of combating infectious agents. Being a modern, minimally invasive method with an almost complete absence of side effects, the use of low-intensity laser radiation can be considered as one of the alternatives to traditional antibiotic therapy. At the same time, the search for new chemical compounds with pronounced antimicrobial activity is also promising. The impact of low-intensity laser of the red spectrum (λ = 660 nm) on the sensitivity of a clinical isolate of Staphylococcus aureus to newly synthesized halogen- and chalcogen-halogen-containing derivatives of the quinazoline and benzothiazole series was studied using the serial microdilutions method with the determination of minimal inhibitory and minimal bactericidal concentrations. To evaluate the antimicrobial properties of the investigated substances in dynamic, we investigated their activity two months after the synthesis with subsequent comparison to freshly synthesized compounds. The research results indicate that the most pronounced antimicrobial effect was shown by trichlorotelluromethylthiazoloquinazolinium chloride and propargylthiobenzothiazolium hexachlorotellurate. Evaluating the dynamics of the activity of the studied compounds, it was noted that the absolute majority of substances retained their properties, which indicates their stability. When evaluating the effect of irradiation of microbial inoculum with low-intensity laser radiation on the susceptibility to chemical compounds, an increase in the sensitivity of irradiated microorganisms to some investigated chemicals was noted compared to similar non-irradiated microbial suspensions. In the case of 2 out of 9 studied chemical compounds we noted a 2- to 4-fold decrease in the minimal inhibitory concentration for irradiated microbial suspensions. A decrease in the minimal bactericidal concentration after irradiation was noted for one of the substances. In the cases of butynylthiobenzothiazolium hexabromotellurate, and tribromotelluromethylthiazoloquinazolinium bromide, decrease of both minimal inhibitory and minimal bactericidal concentrations in the irradiated inoculum was observed. The above shows that low-intensity laser radiation under certain parameters increases the susceptibility of microorganisms to antimicrobial agents.

The influence of low-intensity laser radiation on the blood parameters of cows under technological stress

The study of clinical-hematologic parameters under technological stress at cows and the effect of low-intensity laser radiation (LILR) under technological stress was carried out. The study was carried out on high-yielding Holstein black-breed cows which were modeled technological stress and subsequently exposed to LILR on the withers or ear with exposure time of 5 or 15 depending on the group of animals. LILR with a wavelength of 830 nm was used. The study of hematological parameters of blood under technological stress in animals showed an increase in the number of leukocytes up to 14 days, a decrease in the number of erythrocytes and hemoglobin by 3 days of the experiment relative to the values of intact animals. At action of LILR under technological stress the increase of erythrocytes and hemoglobin in blood was registered. The content of leukocytes in blood corresponded to the level of intact animals, their functional activity increased. The concentration of reduced glutathione in animals under technological stress was decreased throughout the entire observation period. When using LILR under technological stress in animals, changes in reduced glutathione were less pronounced compared to animals after technological stress. When cows were exposed to LILR on the withers, an increase in the content of reduced glutathione in blood was registered. The obtained results indicate that the changes in blood parameters are directed to the activation of compensatory-adaptive reactions of the organism under the action of LILR under technological stress.

In vitro effects of low-intensity infrared laser irradiation on oxidative stress biomarkers in blood samples from diabetes mellitus patients

PurposeThe biological effects of low-intensity laser irradiation on oxidative damage and free radical-induced processes in the blood of patients with diabetes mellitus have not been studied yet. The aim of this study was to evaluate the time- and dose-dependent effects of low-intensity infrared (IR) irradiation on biomarkers of lipid peroxidation, oxidative modification of proteins (aldehydic and ketonic derivatives), and total antioxidant capacity in blood of healthy individuals and patients with diabetes mellitus in vitro. MethodsBlood samples were collected from the diabetes patients and healthy individuals and exposed to low-intensity infrared irradiation in vitro. The samples were exposed to 1-, 5-, and 9-min IR laser pulses with the wavelength of 0.89 nm at different pulse repetition frequencies (0; 50; 150; 600; 1500 Hz) and moderate radiation power (0; 50; 100 mW). The biomarkers of oxidative stress, i.e. 2-thiobarbituric acid reactive substances (TBARS), aldehydic and ketonic derivatives of oxidatively modified proteins, and total antioxidant capacity (TAC), were measured and analyzed in the samples of the exposed whole blood. ResultsCompared with the blood from the healthy controls, the lipid peroxidation analysis revealed 7 combinations of irradiation impact in the blood from the diabetes patients resulting in a decrease in lipid peroxidation processes, especially after the 9-min exposure. The analysis of oxidatively modified proteins demonstrated that laser light is mainly involved in the prevention of protein oxidation at moderate radiation power (50 and 100 mW), different pulse repetition frequencies (0; 50; 150; 600; 1500 Hz), the 9-min exposure and resulting in a decrease in lipid peroxidation processes. The analysis of the total antioxidant capacity revealed a predominantly positive effect of the low-intensity infrared irradiation. ConclusionsThese findings suggest potential involvement of blood dysfunction in the pathologic process of diabetes and provide insight into potential therapeutic targets of low-intensity IR laser irradiation for diabetes patients.

Experimental Determination of the Stability of the «Flamena» Gel Pharmacological Structure under the Influence of Low-Intensity Laser Radiation

In modern dentistry, the problem of the prevention and treatment of peri-implantitis is relevant. Proposed methods of treating patients with peri-implantitis do not stop the pathological process with the possibility of achieving long-term remission. Liposomal complexes with dihydroquercetin make it possible to influence the pathogenetic links of the inflammatory process in periodontal tissues with the prospect of normalizing blood circulation and regeneration processes in the affected area. It has been established that the complex simultaneous effect of low-intensity laser radiation and a pharmaceutical (laserophoresis) provides the possibility of more significant penetration of the drug components into periodontal tissues. The study of the laserophoresis of the liposomal complex with dihydroquercetin in the treatment of patients with peri-implantitis is relevant. However, in the modern literature, there is a lack of studies on the effect of low-intensity laser radiation on the pharmaceutical structure of drugs based on the above-mentioned basis.

Features of laser therapy of venous trophic ulcers of the lower extremities in outpatient surgery

Introduction. Trophic ulcers of venous etiology are a severe complication of chronic venous insufficiency of the lower extremities. At the same time, in most cases, ulcerative defects do not heal for a long time and are prone to recurrence, which leads to a significant deterioration in the quality of life of patients.Aim. To clarify the features of the effect of low-intensity laser radiation to determine the optimal conditions for conservative treatment of venous ulcerative defects.Materials and methods. A randomized prospective clinical trial was performed in which 126 patients with chronic venous insufficiency were monitored. During the conservative treatment of 44 patients of the comparison group, phlebotropic drugs, compression therapy, wound coverings and ointment dressings were used in the conservative treatment of trophic ulcers. In 82 patients of the main group, lowintensity laser radiation was used along with the methods described above.Results. Laser therapy in the complex treatment of venous trophic ulcers helps to reduce the number of microbial bodies vegetating on the surface of ulcerative defects, but does not significantly affect the sensitivity of microflora to antibacterial drugs. Low-intensity laser radiation certainly contributed to a significant and earlier increase in the number of neutrophils, monocytes and macrophages on the surface of trophic ulcers, as well as activation of phagocytic activity of cells.Conclusion. Low-intensity laser radiation improves the results of conservative treatment of venous trophic ulcers, does not adversely affect the sensitivity of the isolated microflora to antibiotics and does not have a “direct” bactericidal effect.

Effect of laser radiation and sodium fluoride on the reactivity of prepared teeth

BACKGROUND: The use of esthetic restorations (metalceramic and metal-free) provides for the preparation of teeth within the enamel and surface or middle layers of the dentin, which entails an increase in the reactivity of teeth. One of the promising directions for increasing the resistance of the prepared teeth in the orthopedic treatment of patients using esthetic dental restorations is the combined and consistent use of fluorides and low-intensity laser radiation.
 AIM: To examine the effect of low-intensity laser radiation and sodium fluoride on the reactivity of dissected vital teeth.
 MATERIAL AND METHODS: A 2% aqueous solution of sodium fluoride was administered by electrophoresis with a portable device Desensitron-II. For laser exposure, a dental laser device Optodan was used in the first (anti-inflammatory) mode of operation. The research material was the results of orthopedic treatment of 120 patients aged 2039 years (57 men and 63 women). To assess the initial state of teeth reactivity and the effect of the therapeutic measures, the sensitivity of the teeth to temperature (thermoreactivity of the teeth), mechanical (sliding tangent test), and electrical (electrodontometry) influences was investigated.
 RESULTS: In the third group, the studied indicators before fixation demonstrated the maximum improvement in indicators relative to the data obtained in other groups.
 CONCLUSIONS: The combined sequential effect of low-intensity laser radiation and sodium fluoride reduces the reactivity of the prepared teeth while significantly improving the indicators of electrical excitability of the pulp and increasing the pain threshold in relation to temperature and mechanical stimuli.

COMPLEX EFFECT OF LOW-INTENSITY LASER RADIATION AND POTASSIUM CHANNEL PEPTIDE INHIBITOR ON MELANOMA CELL SURVIVAL

Melanoma is characterized by an aggressive development and a large number of metastases during diagnostics. Photodynamic therapy (PDT) is used to treat this type of cancer. However, the accumulation of photosensitizers is observed not only in malignant tumors, but also in high metabolic rate organs. Shortcomings of melanoma therapy can be eliminated using the complex effect of laser radiation and local administration of inhibitors of cellular processes.
 The goal. To study the complex effect of low-intensity laser radiation and potassium channel peptide inhibitor on melanoma cell survival.
 Materials and Methods. A875 melanoma cells were exposed to Kappa-theraphotoxin-Gr1b toxin and laser irradiation. The authors examined the level of apoptosis and necrosis in cells using fluorescence microscopy techniques. The xCELLigence system was used to assess the cytotoxic response of A875 melanoma cells.
 Results. The maximum number of apoptotic and necrotic cells was observed in the group of patients with A875 tumor cells exposed to a combination of Kappa-TRTX-Gr1b toxin and laser radiation (wavelength=1265 nm). This is due to the inhibition of potassium channels of intracellular cell membranes by Kappa-TRTX-Gr1b peptide, which are associated with the apoptosis.
 Conclusion. Selective potassium channel inhibition under pathological processes can be regarded as a significant supplement to the superficial malignant neoplasm complex therapy. The combination of toxin and irradiation will make it possible to potentiate their action and avoid the main PDT disadvantages. This approach unites the benefits of the local administration and precise exposure on the malignant tumor.

The effect of low-intensity laser radiation on the healing of microbial-contaminated soft tissue and bone wounds in an open fracture (experimental study)

Influence of low intensive laser irradiation (LILI) on the healing of the infected osseous and soft tissue wound in experimental open fracture was studied using transmission and electron microscopy. LILI provided antimicrobic affect on the infected wounds increasing phagocytosis by neutrophiles and accelerating the formation of callus.

ПРИМЕНЕНИЕ ИМПУЛЬСНОГО ИНФРАКРАСНОГО НИЗКОИНТЕНСИВНОГО ЛАЗЕРНОГО ИЗЛУЧЕНИЯ В КОМПЛЕКСНОМ ЛЕЧЕНИИ БОЛЬНЫХ С ПОСТИНСУЛЬТНЫМИ КОГНИТИВНЫМИ НАРУШЕНИЯМИ

The results of treatment of 60 patients with a verified diagnosis of ischemic stroke, acute period, with a motor deficit in the form of hemiparesis, with cognitive impairment, are presented; patients were distributed by random sampling into two groups: group 1 (30 people), the standard treatment was supplemented with a course of laser therapy, group 2 (30 people) received only the standard course of treatment. In patients, the effect of low-intensity laser radiation on the cognitive functions, short-term memory, attention, and mental abilities was studied. Efficiency was assessed using the MMSE scale, the Number Memory, Visual Memory Tests, and the method of finding numbers on Schulte Tables. It was found that the inclusion of low-level infrared laser radiation in a standard medical complex reduces the level of cognitive impairment by 2.3 times, increasing short-term memory by 1.6 times, attention by 2.2 times (p < 0.05). In the comparison group, the dynamics of these indicators is significantly lower. The inclusion of pulsed infrared low level laser therapy (LLLT) in the complex rehabilitation of patients with ACVA is a necessary link in neurorehabilitation measures as a method for compensating the impaired brain functions.

Extended experiment on potable water irradiated with low-intensity laser pen and Solfeggio scale frequencies

In previous article, we reported initial findings based on small experiment on potential use of salt-water as cheap source of renewable battery with various kind of metals as anode and cathode. The purpose of these experiments is to find out which combination of anode and cathode is capable to generate the best performance in terms of electric voltage. Following those previous reports, this writer also conducted several tests to see effect of low-intensity laser irradiation on potable water. While the first series of our experiment clearly indicated possible laser cooling effect of low intensity laser irradiation of potable water and salt water, the following extended experiment on low intensity laser irradiation shows a rather mixed result. In a series of initial experiment, laser irradiation with laser pen gave lower electric potential (down to several minus milli Volt to the Voltmeter reading), but later on after more than 180 sec of irradiation, it shows increased positive milli Volt of electric potential of the system. In this experiment, we found that combination of low-intensity laser irradiation and Solfeggio scale frequencies applied to potable water shows dim result.

CHARACTERISTICS OF THE INTERACTION BETWEEN LOW-INTENSITY LASER RADIATION AND ERYTHROCYTES

The results of the effect of low-intensity laser radiation on lipid peroxidation and the sorption capacity of erythrocyte membranes under stress are presented. The membrane-stabilizing effect of low-intensity laser radiation and a decrease in the rate of free radical processes are noted, which indicates the prospects of using laser radiation as an activator of the body's natural antioxidant systems. An increase in the sorption capacity of erythrocyte membranes proves the advisability of using low-intensity laser radiation to increase the adaptive capacity and to protect the body from stress.

Low-intensity laser radiation effect on protein metabolism in young sheep

The demand for sheep products these days becomes higher, as evidenced by the tendency to increase small ruminants in the world and Russia. It leads to manufacturers’ desire to get products as quickly as possible, without worrying about their quality. Various medications are used, which later, when eating animal meat, can have a detrimental effect on the human body. These studies aimed to study the effect of low-intensity laser radiation on the sheep’s organism and obtain ecologically clean and high-quality mutton. For this, during the experiments, the blood of animals was studied. An essential role of protein metabolism in the animal body is blood serum proteins, qualitative and quantitative characteristics. They are the primary plastic material of tissues and organs and carry out regulatory, catalytic, and immunobiological functions. The growth rate is closely related to protein deposition. Individual development is determined by the constant exchange of substances and energy between the body and the external environment. However, each animal species has its metabolic traits, and, in particular, it concerns the transformation of proteins. Age-related biochemistry of protein metabolism has significant material in disclosing the fundamental biochemical constants of metabolism in animals’ bodies. Simultaneously, the study of the peculiarities of protein metabolism is one of the main links in the knowledge of the biochemical essence of each period of sheep’s life.

THE EFFECT OF LOW-INTENSITY LASER RADIATION ON THE BIOCHEMICAL STATUS AND MILK PRODUCTIVITY OF COWS AGAINST THE BACKGROUND OF TECHNOLOGICAL STRESS

Technological stress arising under the influence of factors related to the methods of keeping cattle and elements of technology has an adverse effect on the health of animals and their productivity. The development of methods that increase the natural resistance of animals and have an adaptogenic effect under stress is an urgent problem of animal husbandry and veterinary medicine. The aim of the work was to study the effect of low-intensity laser radiation on the biochemical parameters of blood and milk productivity of cattle in an industrial complex. Material and methods. Experimental studies were conducted on highly productive black-and-white cows. As an anti-stress factor, low-intensity laser radiation with a wavelength of 830 nm was used, the exposure time was 5, 15 minutes in the ear and withers area. The content of total protein, glucose, urea, creatinine, cholesterol, and transaminase activity were determined in the blood. Studying the milk productivity, we determined the fat-milk content, protein-milk content, the content of lactose and solids in milk. Results. In animals under conditions of technological stress, the indicators of milk productivity were reduced, the content of total protein, creatinine, urea, cholesterol was reduced, the content of glucose and transamination enzymes was increased compared to the indicators of the intact group. The use of low-intensity laser radiation in the ear area for 15 minutes and withers for 5 minutes contributed to the normalization of the studied biochemical blood parameters in animals. The best indicators of milk productivity were obtained in cows that were exposed to low-intensity laser radiation for 5 minutes. Conclusion. The results of the experiment indicate that high-intensity laser radiation has a positive effect on the physiological reserves of the body, which allows it to be used to achieve optimal productivity of animals.

The current state of Atlantic salmon reproduction in the Keret River, White Sea basin, and how it can be increased

The Keret River is one of large salmon rivers in the White Sea basin. The estimated abundance of Atlantic salmon in the river began to decrease in 1991, reaching a maximum in 1997 due to the abruptly increased illegal fishing level and the invasion of juvenile Atlantic salmon by the monogeny of Gyrodactylus salaris. The studies conducted have shown that the salmon population in the Keret River is depressed and that the average distribution density of juveniles aged 1+ and older is 1.6 fish / 100 m2. Atlantic salmon reproduction on fish hatcheries is important for maintaining the population abundance (farm individuals make up over 50% of the population). Artificial Atlantic salmon reproduction in the Republic of Karelia’s rivers is carried out by the Karelian Branch of Glavrybvod FGBU at the Kem and Vyg fish hatcheries. The fish hatcheries could achieve better results by updating the juvenile breeding technology. An example of a new high-intensity biotechnology is the use of physical factors controlling the vital activity of fish in the various periods of ontogenesis. The study of the effect of low-intensity laser radiation in the early stages of ontogenesis has shown that red light over a certain dose range enhances the adaptability of fish by decreasing mortality and accelerating growth, thus decreasing the loss of fish products in fish hatcheries.

Analgesia by laserophoresis of herbal extracts in treatment of patients with lumbosacral dorsopathy

BACKGROUND: The article describes the pharmaco-physiotherapeutic method of analgesia for pain in the lumbosacral spine. The possibilities of introducing the method of transcutaneous analgesia into therapy using laserophoresis of a biological complex of water extracts of field horsetail and St. John's wort are shown. These phytoextracts were used in the laser phoresis method due to the fact that they have analgesic, decongestant and adaptogenic effects, as well as reduce the metabolic activity of chemical agents, including inflammatory ones.
 AIMS: Study of the analgesic effect of the developed method of phytocomplex laserphoresis in comparison with the standard scheme of nonsteroidal anti-inflammatory drugs.
 MATERIAL AND METHODS: The study included 48 patients with a confirmed diagnosis of lumbosacral dorsopathy accompanied by pain syndrome were under observation. The main group consisted of 28 people who underwent phytocomplex laserphoresis procedures as a local treatment. The control group consisted of 20 people, where the standard treatment with NSAID drugs was prescribed. The study included observation and control before and after the course of treatment conducted in a polyclinic. To assess the severity of pain was applied to the visual analogue pain scale and the method of tensoalgometry.
 RESULTS: All patients underwent treatment well, there were no cases of interruption of the course of treatment. As a result of dynamic analysis of indicators performed before and after treatment, pain scale and tenzoalgometry indicators showed results indicating faster and more effective relief of pain in patients of the main study group. When summing up the results of treatment, the improvement in pain scale indicator showed an 83% improvement in the main group (p 0.05), while the improvement in the control group was only 39%. According to the control tensoalgometry, in the main group, pain thresholds improved by 32% (p 0.05), while in the control group the improvement was only 12%.
 CONCLUSION: The resulting analgesic effect is formed due to the anti-inflammatory effect of low-intensity laser radiation, as well as the therapeutic properties of the phytocomplex used, which can replace the use of NSAIDs for analgesia of pain in the lumbosacral region.

PHOTOBIOMODULATORY EFFECT OF LOW-INTENSITY LASER RADIATION ON MULTICELLULAR SPHEROIDS

Photobiomodulatory effects of low-intensity laser radiation (LILR) in cells cultured in standard, two-dimensional conditions are well established. Conversely, the characteristics of this effect in three-dimensional (3D) cultures, which are currently recommended due to the greater similarity with cellular behavior in vivo, have not yet been widely investigated. The objective of this work was to analyze the biomodulator effect of LILR, on the wavelength (λ) of 685 nm, on the constitution process and on the viability of cells cultured as multicellular spheroid (MSs). For this, agarose molds containing microwells were seeded (2x105 cell/ml) with osteogenic precursor cells (OPCs - MC3T3-E1) and kept under ideal culture conditions. The molds were irradiated for five consecutive days with doses of 0.5, 1.0 and 1.5 J/cm², the first irradiation being performed immediately after sowing. The process of constitution of MSs was analyzed and the cultures were submitted to the cell viability test. The results demonstrated that the LILR at λ 685 nm exerted a dose-dependent biomodulatory effect on cell metabolism and on the process of constituting the MSs of OPCs. These results demonstrate the potential of photobiomodulation to contribute to the process of constituting MSs, which can be explored in the strategies of multicellular spheroids therapy used in regenerative medicine and bioprinting.

Особенности телосложения молодняка овец при биофизических методах воздействия

Aim. Study the effect of low-intensity laser radiation on the genotype's young sheep (poll Dorset x North Caucasian meat-wool) and their exterior. Material and Methods. Zootechnical, biophysical, statistical and other methods were used in research. Experimental animals were exposed to an STP laser apparatus, the action of which is based on the use of low-intensity laser radiation in the infrared region of the spectrum. The study of measurements of animals at the age of 5 and 7 months was carried out and physique indices were calculated. Results. Comparing measurements in the studied animals at 5 months of age revealed the advantage of lambs of groups II and III over the control, in almost all main parameters. However, the experimental groups' young animals were characterized by higher rates of breast measurements. Thus, the superiority of lambs of groups II and III over animals of the control group were: in chest depth 2.1-3.3%, width – 4.4-7.1%, girth – 1.2-4.1% (P < 0.001). A similar trend was established due to measurements of the studied animals' physique at the age of 7 months. At the same time, a clear advantage in terms of measurements characterizing breast development was found in young animals of the second and, especially, in the third group: in chest depth by 3.8-7.4%, width by 4.8-9.1%, girth – 3,7-9.0%, with a significant difference (P < 0.05; P < 0.001). It was also found that the greatest value of the breast index at 5 months of age was typical for animals of groups II and III (77.6-78.6%), which is 1.8-2.8 abs. percent (P < 0.001). However, with age, the difference in the value of this indicator between the groups decreased. Therefore, at the age of 7 months, young animals of groups II and III surpassed animals of group I in this indicator by 0.8-1.5 abs. percent. Conclusion. Thus, it was found that the best ratio of most body articles and body build indices, characterizing them as animals with well-pronounced meat forms, were animal groups II and III.

Two dimensional automatic active shape model of degenerative disc repaired by low-intensity laser.

Intervertebral disc degeneration is the main factor causing low back pain, and the related long-term treatment can improve the situation of degeneration. This study aimed to investigate the effect of low-intensity laser irradiation on the repair of degenerative intervertebral disc by two dimensional automatic active shape model (2D-AASM). Nine Bama miniature pigs were randomly divided into three groups: control group (Con), model group (Mod) and laser treatment group (Las). After one month, the discs were treated with low-energy laser for another month. MRI was performed for one month, and the statistical shape model and 2D-AASM of intervertebral disc were established based on the minimum description length method. The model established by the proposed method is more accurate and the segmentation result is more accurate. From the segmented T2-weighted image, the signal intensity of the Mod group decreased significantly, and the signal intensity in the Las group was moderate and high compared with the Mod group. The HE staining display the structure of Con group was damaged, and the construction of Las group was restored compared with Mod group. The 2D-AASM method effectively improves the accuracy of intervertebral disc segmentation. The low-intensity laser has a protective effect on the repair of the degenerative intervertebral disc.

Activation of the excretion of radionuclides from the body of fattening gobies and their retention level

The article is devoted to the use of low-intensity laser radiation and pectin containing feed additives in animal husbandry to activate the excretion of radionuclides through the gastrointestinal tract and kidneys of black-motley bulls for fattening in order to reduce their retention in the body. As a result of the studies, the high efficiency of these factors is proved. The specific most effective radiation doses for gobies were determined. Mountain ash berry as a food supplement is recommended to enhance the positive effect of low-intensity laser radiation.

Photoactive effects of low-intensity laser irradiation at the microcirculation system and lymphoid organs

The authors present a review of publications in the scientific and medical literature on modern concepts of mechanisms of low-level laser biostimulating effects at the human body, at microvasculature chains and lymphoid organs. The main aspect of microcirculation photoactivation is the process of microcirculation reconstruction which leads to prolonged improvement in tissue trophism. There are three processes in the basement of biostimulating effect of low-level laser light in the red and near infrared spectral regions at microcirculation: hemomicrocirculation enhancement, activation of capillary neovascularization, lymphocorrection effects at the structure and functional activity of lymphoid organs.