Microalgae is a popular condidate for the sustainable biodiesel due to its high growth rate and lipid content. This paper reviewed the effects of light conditions, including light intensity, photoperiod and spectrum on the lipid accumulation in microalgae and selects the spectrum as the variable. In this paper, the effects of different light emitting diodes (LEDs) spectra such as red, blue and white on the lipid accumulation in two microalgae species were discussed. The results showed that the total lipid content was the highest (13.76% and 31.18% of dry cell weight) in the two microalgae species, Ankistrodesmus falcatus and Chlorella vulgaris, cultured under blue light. The fatty acid methyl esters (FAME) composition was significantly influenced by the light spectrum