Depth of interaction (DOI) technology can improve the spatial resolution of nuclear medicine imaging system which uses scintillation detectors such as Positron Emission Tomography (PET). In this paper, a prototype detector module with DOI capability is established to make complementary characteristic tests on an existing method and to improve the experimental performance using the same method. We investigate the gamma incident surface and incident angle effects on the positioning method with our model in simulations and evaluate its 3-D positioning results in experiment. It shows that the positioning results are highly affected by the gamma incident surface and incident angle. The 137Cs energy resolution is 12.1% and the DOI resolution is estimated at 2.26 mm in average by our detector in experiment.