Objective This study evaluated the influence of different mechanical and chemical surface treatments alone and combined with silane on the bond strength (BS) of glass fiber posts (GFPs) using self-adhesive resin cement. Methods Eighty-four single-rooted bovine teeth (six groups, n = 14) were submitted to BS analysis after GFP cementation. The treatments applied in the studied groups were no surface treatment (control), silane (S), 24% hydrogen peroxide (PER), 24% hydrogen peroxide and silane (PER + SIL), blasting with 50 μm aluminum oxide particles (BLAST), and blasting with 50 μm aluminum oxide particles and silane (BLAST + SIL). Results BS differed significantly among groups (p < 0.001). It was higher in the SIL (10.5 ± 3.5 MPa), BLAST + SIL (11.5 ± 3.2 MPa), and PER + SIL (11.6 ± 4.6 MPa) groups than in the control (6.5 ± 2.9 MPa), BLAST (8.6 ± 4.0 MPa), and PER (7.1 ± 2.8 MPa) groups, with no significant difference among groups receiving silanization. Cement post adhesive failure was more common in the SIL, BLAST, and PER + SIL groups, and cement-dentin adhesive failure was more common in the control, BLAST + SIL, and PER groups. Conclusion These results show that silane application alone increases BS.