Effect Of Different Times Research Articles

To see, or not to see… pathogens in virtual reality hand hygiene training.

ViRTUE, a virtual reality (VR) hand hygiene trainer, offers users the option of visualizing pathogen transfers during virtual patient care either in "real-time" or at the end of a level as a "summary" visualization. In this study, we aimed to evaluate the effect of different timings of pathogen visualization ("real-time" vs "summary") on in-trainer performance and user's immersion. The study included first-year medical students undergoing hand hygiene training with ViRTUE, randomized to one of three visualization set-ups: set-up 1 ("on-off-off", with "real-time" visualization at the first level only, and "summary" visualization at level 2 and 3), set-up 2 ("off-on-off"), and set-up 3 ("off-off-off"). In-trainer performance was defined by number of pathogen transmission events (=contaminations) in level 3. The virtual experience of user's (among others: immersion) was assessed with a questionnaire. 173 medical students participated in the study, with 58, 54, and 61 assigned to set-up 1, set-up 2, and set-up 3, respectively. Users assigned to set-up 3 with "summary" visualization at all levels, performed best with 1.02 (standard deviation (SD) +/- 1.86) contaminations, compared to 2.34 (SD +/- 3.09) and 2.07 (SD +/- 2.52) contaminations of users assigned to the other set-ups. "Summary" visualization at all levels also resulted in higher immersion of users. "Real-time" visualization of pathogen transmission during VR hand hygiene training with ViRTUE may negatively affect in-trainer performance and user immersion. This emphasizes the importance of pilot testing the effect of VR-based trainings in order to understand their impact on users.

Does timing of tocilizumab administration affect mortality in COVID-19? A Scottish multicentre retrospective cohort study

BackgroundThe optimal timing of tocilizumab treatment during the disease course of COVID-19 has yet to be adequately defined in the context of randomised controlled trials and the effect of tocilizumab...

Effects of different timing and rate of glyphosate application on the residue level, grain quality, and processing performance of two Canadian malting barley varieties

Effects of different timing and rate of glyphosate application on the residue level, grain quality, and processing performance of two Canadian malting barley varieties

Effect of different timing of water deficit combined with foliar application of ascorbic acid on physiological variables of sour passion fruit

The objective of this study was to evaluate the effects of deficit irrigation treatments during different phenological stages and foliar application of ascorbic acid on gas exchange, photosynthetic pigments, chlorophyll fluorescence, electrolyte leakage, relative water content and yield of ‘BRS GA1’ sour passion fruit. Treatments were distributed in randomized blocks, in a split-plot scheme, with plots consisting of six deficit irrigation treatments, based on crop evapotranspiration – ETc (irrigation with 100% ETc - FULL; irrigation with 50% ETc in the vegetative stage - VEG; flowering stage - FLO; fruiting stage - FRU; successively in the vegetative/flowering stages - VEG + FLO and vegetative/fruiting stages - VEG + FRU) and subplots represented by three concentrations of ascorbic acid - AsA (0, 0.5 and 1.0 mM). Water deficit induces changes in photosynthetic activity across all developmental stages of sour passion fruit, resulting in reduced yield in the period of 160 and 200 days after transplanting. Notably, flowering exhibits the greatest physiological acclimatization to water deficit conditions, albeit with more pronounced losses in the yield. When water deficit occurs during the vegetative and fruiting stages and is coupled with foliar application of ascorbic acid at 0.5 mM, there is an enhancement in the synthesis of photosynthetic pigments in ‘BRS GA1’ sour passion fruit. Overall, foliar application of ascorbic acid at concentrations of 0.5 and 1.0 mM emerges as a viable strategy to mitigate the adverse effects of water deficit on the physiology and yield of sour passion fruit at 200 days after transplanting.

PSI-5 Evaluation of EhV (surrogate for African swine fever virus) inactivation in corn- and soybean-based feed ingredients and diets at various times and temperatures during storage

Abstract African swine fever virus (ASFV) is a member of the nucleocytoplasmic large DNA viruses (NCLDVs) and remains stable in a variety of environments, including animal feed ingredients as shown in previous laboratory experiments and simulations. Emiliania huxleyi virus (EhV) is another member of NCLDV, which has a restricted host range limited to a species of marine algae called Emiliania huxleyi. This algal NCLDV has many similar morphological and physical characteristics to ASFV which makes it a safe and reliable surrogate for studying ASFV survival and inactivation in feed ingredient matrices in-situ under various time and temperature storage conditions. The objective of the present study was to investigate the inactivation of ASFV in various feedstuffs under a wide range of time and temperature conditions during storage using EhV as a surrogate. Corn- and soybean-based feed ingredients including corn, corn fermented protein, distiller dried grains with solubles, extruded soybean meal, high protein distiller’s grains, solvent-extracted soybean meal, soybean hulls, complete feed, and a positive control (no matrix) were inoculated with EhV (1 × 108 viruses/mL). Negative controls were inoculated with medium containing no EhV for each matrix. To assess the effects of different times and temperatures of storage, all samples were stored at temperatures of 4, 24, and 34 °C for 1, 5, 60, and 120 d. For each timepoint and temperature combination, samples were evaluated for virus presence (qPCR) and viability using a previously validated viability qPCR (v-qPCR) method. On average, we observed a 0.2 log reduction in viable viral DNA content over the 120-d period, with a 0.1 log reduction across varying temperatures. No reductions in EhV viability exceeding 1 log were detected for any matrix and the positive control at any time and temperature combination evaluated. No EhV was recovered from the negative control samples. Given that the experimental error was calculated to be within the range of 1 log, we concluded that storing EhV at 4, 24, or 34 °C for up to 120 d did not result in the inactivation of EhV in any of the ingredients, complete feed, or the control with no matrix. These results demonstrate that ASFV-like NCLDVs is extremely resilient and can maintain viability in various feed matrices during long-term storage and suggest that extending storage time alone may not be an effective mitigation practice against African swine fever virus.

Airport Cab Drivers' Passenger Carrying Strategy Selection Based on Mathematically Derived Theoretical Models

Cab is an important way for passengers to choose to go to the city in the airport. Cab drivers will send customers to the airport. There are two options to carry passengers, choosing to return to the city to carry passengers or entering the pool at the airport to wait for passengers. How the cab driver to decide to rely on the pool waiting time, the airport of a single day by the time of day the flow of passengers, downtown to the airport in the time required, and other factors. To compare the two options and analyze the influencing factors, this paper establishes a theoretical model of mathematical derivation to find out the scope of application of the two types of options, and to establish a decision-making model for cab drivers to choose. Then, using specific airport data, MATLAB is used to fit the curve to the collected data, and the decision value is calculated by applying integrative science to get the cab's time from the city to the airport, to arrive at the decision-making scheme. Finally, the dependency of the relevant factors is analyzed and the effect of different times of day, season, and fuel cost government-related policies on the revenue coefficient is considered using gray correlation analysis.

Dose- and stage-dependent toxic effects of prenatal prednisone exposure on fetal articular cartilage development

Prednisone is frequently used to treat rheumatoid diseases in pregnant women because of its high degree of safety. Whether prenatal prednisone exposure (PPE) negatively impacts fetal articular cartilage development is unclear. In this study, we simulated a clinical prednisone treatment regimen to examine the effects of different timings and doses of PPE on cartilage development in female and male fetal mice. Prednisone doses (0.25, 0.5, and 1 mg/kg/d) was administered to Kunming mice at different gestational stages (0–9 gestational days, GD0–9), mid-late gestation (GD10–18), or during the entire gestation (GD0–18) by oral gavage. The amount of matrix aggrecan (ACAN) and collagen type II a1(COL2a1), and expression of transforming growth factor β1 (TGFβ1) signaling pathway also demonstrated that the chondrocyte count and ACAN and COL2a1 expression reduced in fetal mice with early and mid-late PPE, with the reduction being more significant in the mice with early PPE than that in those with PPE at other stages. Prenatal exposure to different prednisone doses prevented the reduction of TGFβ signaling pathway-related genes [TGFβR1, SMAD family member 3 (Smad3), SRY-box9 (SOX9)] as well as ACAN and COL2a1 mRNA expression levels in fetal mouse cartilage, with the most significant decrease after 1 mg/kg·d PPE. In conclusion, PPE can inhibit/restrain fetal cartilage development, with the greatest effect at higher clinical dose (1 mg/kg·d) and early stage of pregnancy (GD0–9), and the mechanism may be related to TGFβ signaling pathway inhibition. The result of this study provide a theoretical and experimental foundation for the rational clinical use of prednisone.

Investigation of Expanded Human Adipose-derived Stem Cell Dosage and Timing for Improved Defecation Function.

Although certain treatment options exist for intestinal incontinence, none are curative. Adipose-derived stem cells (ADSCs) have emerged as promising therapeutic agents, but most preclinical studies of their effectiveness for anal function have used autologous or allogeneic ADSCs. In this study, the effectiveness, timing of administration, and required dosage of human ADSCs were investigated for clinical application. A 10-mm balloon catheter was used to induce anal sphincter injury in immunodeficient mice in the following experimental groups (n=4 per group): ADSC (injected ADSCs after injury), PBS (injected phosphate-buffered saline after injury), and control (uninjured). The effects of different timing (immediately after injection and 30 days following injury) and number of human ADSCs administered was compared among groups based on defecation status and pathological evaluation. In terms of defecation status, groups receiving ≥1×104 human ADSCs after injection showed improvement. Pathological images showed that compared to the PBS group, the thinnest part of the sphincter was thicker for animals that received ≥1×104 human ADSCs, and fibrosis of the sphincter was notable in those treated with 1×103 human ADSCs or PBS. Furthermore, defecation status was improved by administration of human ADSCs, not only immediately after injury, but also at 30 days following injury. Human ADSC administration in a mouse model of anal sphincter injury was effective. Injection of ≥1×104 human ADSCs was the amount necessary to improve defecation status, an effect detected in both the acute and chronic phases.

Effect of different timings of umbilical cord clamping on the level of CD34+ cells in full-term neonates

Despite the fact that delayed cord clamping (DCC) is recommended by many international organizations, early cord clamping is still widely practiced worldwide. The overarching goal of the DCC practice is to maximize neonatal benefits as achieving higher hemoglobin levels and decreasing the incidence of anemia as well as avoiding the adverse consequences. The current study was conducted to identify the effect of of DCC on the number of CD34+ stem cells in cord blood of full term neonates after two different timings (30 and 60 s after birth). One hundred and three full-term (FT) newborn babies (gestational age 37–40 weeks) delivered by elective cesarean section were randomly assigned into 2 groups: Group 1: babies were subjected to DCC 30 s after birth (50 newborns). Group 2: babies were subjected to DCC 60 s after birth (53 newborns). Neonates in group 2 had significantly higher levels of hemoglobin, hematocrit, total nucleated cells and CD34+ cells compared to those in group 1. The practice of DCC 60 s after birth achieved better CD34+ stem cells transfer in FT neonates than clamping the cord after 30 s.

Do Different Times of The Day Affect Dart Throwing Performance?

Background: Dart sport has become a sport that has recently become widespread among sports branches and its popularity is increasing rapidly day by day. With this popularization, research on darts is increasing. It is stated that athletes can do the training they do at any time of the day with a much better performance at a different time, as in many changing conditions. In addition, at a different time of day, fatigue may be reached later or recovery may occur more quickly. For these reasons, knowing the effect of training at different times of the day is important for both the coach and the athlete. Objectives: The aim of this study was to investigate the effect of different times of the day on throwing performance in darts, an indoor skill in which focus, attention, concentration are at the forefront and environmental factors are less effective than outdoor skill. Methods: In this study, 16 dart athletes (8 boys, 8 girls) with a sport age of 2 - 3 years and an age of 12 - 14 years, who did not have any health problems in Mardin province, participated voluntarily: 2 m and 2.37 m from two different distances at 8.00 and 17.00, 1 day apart. Before the dart throws, the Daily Rhythm Determination Scale for Children was administered to the participants. The center of the dartboard was placed at eye level for each subject. After the throws, the Perceived Difficulty Scale was applied. In the analysis examining the relationship between perceived degree of difficulty, morningness-eveningness and throwing, the subjects’ evening throwing scores and evening perceived degree of difficulty scores were examined; a negative and significant relationship was observed in the eveningist group (r = -1, P = 0.01). No significant relationship was observed in the morning group. Results: In the study, a statistically significant difference was found between the subjects’ morning and evening throws at 2 m and 2.37 cm. In both morning and evening groups, it was determined that the throwing performance increased in the evening measurements. Conclusions: As a result, this study concluded that there is an effect of time of day in dart sport and that dart throws made in the evening give better results than dart throws made in the morning. Based on these results, it is recommended that a program should be made by the coaches considering the school programs and daily routines to reduce the effect of time of day. It is thought that this study will be important for chronobiological researches to reveal the factors affecting performance as well as the effect of time of day in darts sport.

Effect of Different Timing of Local Brain Radiotherapy on Survival of EGFR-Mutated NSCLC Patients with Limited Brain Metastases.

(1) Background: Epidermal growth factor receptor (EGFR) tyrosine kinase inhibitors (TKIs) have been the first line therapy for EGFR-mutant lung adenocarcinoma (LAC) patients with brain metastases (BMs). However, the role and the optimal time of brain radiotherapy remains controversial. We aimed to investigate the role of upfront brain stereotactic radiotherapy (SRS) and the impact of deferral radiotherapy on patients' clinical outcomes. (2) Methods: We retrospectively studied 53 EGFR-mutant LAC patients with limited synchronous BMs between 2014 and 2020 at our institute. The limited BMs was defined with one to four BM lesions, with a maximal size of ≤4 cm. Patients were categorized into two groups: upfront brain SRS (upfront RT) and upfront TKIs. The intracranial progression-free survival (iPFS), progression-free survival (PFS), and overall survival (OS) between groups were analyzed. (3) Results: The median iPFS (21.0 vs. 12.0 months, p = 0.002) and PFS (20.0 vs. 11.0 months, p = 0.004) of the upfront RT group was longer than that of the upfront TKI group. There were no significant differences in median OS (30.0 vs. 26.0 months, p = 0.552) between the two groups. The upfront RT group is less likely to suffer from intracranial progression of the original sites than that of upfront TKIs during the disease course (36.1% vs. 0.0%, p = 0.025). Multivariate analysis showed that the Karnofsky Performance Scale and the presence of synchronous meningeal metastases were associated with overall survival. (4) Conclusions: Compared with upfront TKI, the combination of upfront SRS with TKIs can improve the iPFS and PFS in EGFR-mutant LAC with synchronous BMs. The addition of upfront brain SRS was useful for the original intracranial metastatic lesions.

Appropriate timing of treatment contributes to better root development of impacted anterior teeth in children

This study investigated the effects of different timings of orthodontic treatment on the root development of impacted anterior teeth in children. The cone-beam computed tomography (CBCT) data of 45 children with impacted anterior teeth were divided into unformed root (UR) group or basically formed root (BFR) group to evaluate root length (RL) and root growth length (RGL) of impacted teeth and contralateral nonimpacted teeth pretreatment and posttreatment. In addition, 22 patients with impacted dilaceration were selected to assess the effects of the crown-root angle and root development stage on RL and RGL. The Student t test, Wilcoxon test, analysis of variance, and multiple linear regression analysis were used for statistical evaluations. The RL of treated impacted teeth pretreatment and posttreatment was significantly shorter than contralateral nonimpacted teeth values (P<0.05). Posttreatment, the RL and RGL of impacted teeth of the UR group were significantly greater than those of the BFR group (P<0.05). The RGL of the dilacerated root in the UR group was considerably higher than in the BFR group (P<0.05). The larger crown-root angle group had a longer posttreatment RL (P<0.05). Multiple linear regression analysis revealed that the Nolla stage of impacted teeth and RL of contralateral teeth pretreatment significantly influenced the RL of impacted teeth posttreatment. Prompt orthodontic treatment is necessary for children with impacted anterior teeth to release the impacted state and achieve better root development. The root length of a dilacerated tooth continued to develop under treatment, but the crown-root angle partly constrained it.

SOWING TIME OPTIMIZATION FOR INSECT PEST MANAGEMENT AND ENHANCED CROP YIELD IN COTTON

Sowing time has a significant role in minimizing insect pest damage by disrupting synchronization between host plant and insect life cycle. The effect of different times of sowing viz. April, May and June designated as early, intermediate and late was determined on the ability of four cotton genotypes (NIA-30, NIA-88, NIA-98 and Sadori) to resist sucking insect pests and bollworms. Cotton genotypes were sown on the 15th of each month. The results showed that the planting time had pronounced effect on the infestation of insect pests. April sown cotton had a higher infestation of jassid compared to May and June sown cotton. The occurrence of thrips was recorded highest in the May sowing followed by April. Infestation of whiteflies and spotted bollworm was negligible on all the genotypes and were below the economic threshold levels during the entire period of observation. Furthermore, April sown cotton showed the higher infestation of spotted and pink bollworms. Among the tested genotypes in different sowing times, they exhibited similarity in insect pest abundance, except for jassid population which significantly differed in May sown genotypes. Concerning yield, April sowing yielded the most at 2107.7 kg/ha, followed by May (1917.6 kg/ha) and June (801.7 kg/ha). Notably, NIA-88 followed by Sadori consistently yielded the highest across all sowing times compared to the other two genotypes. This study indicates that sowing of cotton varieties in April is recommended for achieving higher yields.

The Effect of Different Time on Temperature, pH, and Disolved Oxygen in Indoor Hatchery

The hatchery is a biosecurity system in the form of a water quality control system and aquatic environmental health. Hatchery determines the success or failure of spawning. Temperature, pH and dissolved oxygen are key water quality parameters that control the distribution and distribution of organisms in the waters. Temperature, pH and dissolved oxygen in waters are key water quality parameters that control the distribution and distribution of organisms in the waters. This study aims to describe fluctuations in water quality, namely temperature, pH, and DO in the indoor hatchery based on different times. The design used was a RAL with 3 treatments and 4 repetitions, namely P1: observing water parameters every 1 hour (6, 7, 8, 9, 10, 11, 12, 13, 14, 15, 16, 17, 18, 19, 20, 21, 22, 23, 24 WIT); P2: observation of water parameters every 2 hours (6, 8, 10, 12, 14, 16, 18, 20, 22, 24 WIT); and P3: observation of water parameters every 4 hours (6, 10, 14.18, 22 WIB). The results showed that in general the temperature, DO and pH values ​​in the indoor hatchery did not fluctuate significantly. However, during the observation at 14 to 16 WIB at P1 there was an increase in temperature and DO, on the contrary there was a decrease in the pH value. When DO increases, the pH appears to decrease. At P2 there was a decrease in temperature values ​​at 6 to 8 WIB, while the DO and pH values ​​were stable. Furthermore, at P3, it can be seen that the temperature, DO and pH values ​​are more stable.

Cover cropping impacts on soil water and carbon in dryland cropping system.

Incorporating cover crops into the rotation is a practice applied across many parts of the globe to enhance soil biological activities. In dryland farming, where crop production is highly dependent on rainfall and soil water storage, cover cropping can affect soil water, yet its effects on soil hydrological and biological health require further investigation. The objective of this study was to evaluate the effect of different timing of summer sorghum cover crop termination on soil water, total and labile organic carbon, arbuscular mycorrhizal fungi and their mediating effects on wheat yield. Through on-farm trial, soil characteristics along with wheat biomass, yield and grain quality were monitored. In comparison with the control (fallow), the early terminated cover crop was the most effective at retaining greater soil water at wheat sowing by 1~4% in 0-45cm soil profile. An increase in water use efficiency, yield and grain protein by 10%, 12% and 5% was observed under early termination. Under late terminated summer cover crop, there was 7% soil water depletion at wheat planting which resulted in 61% decline in yield. However, late-terminated cover crop achieved the greatest gain in soil total and particulate organic carbon by 17% and 72% and arbuscular mycorrhizal fungal Group A and B concentration by 356% and 251%. Summer cover crop incorporation resulted in a rapid gain in labile organic carbon, which constituted hotspots for arbuscular mycorrhizal fungi growth, conversely, fungal activities increased labile organic carbon availability. The combined effect of increased soil water at sowing and over the growing season, organic carbon, and microbial activities contributed to greater yield. The findings suggest that summer cover cropping with timely termination can have implications in managing soil water at sowing time and enhancing soil water storage during the season, soil carbon, and facilitating microbial activities while enhancing productivity in the dryland cropping system.

Analysis of povidone iodine, chlorhexidine acetate and polyhexamethylene biguanide as wound disinfectants: in vitro cytotoxicity and antibacterial activity

ObjectivesEven though disinfectants are commonly used in clinical practice and daily life, there are few studies on their antibacterial ability and cytotoxicity, which are closely related to the safety and...

Effect of Different Concentrations of IBA and Time of Taking Cutting on Propagation of Black and White Myrtle (Myrtus communis L.) Cuttings

This study aimed to evaluate the effect of different time of taking cutting and IBA concentrations on the rooting of Myrtus communis L. White and black myrtle types were used as plant materials. The cuttings were obtained from Batı Akdeniz Agricultural Research Institute (BATEM) collection garden. The effects of 0 (control), 500, 1000, 2000, 4000 and 8000 mg L-1 doses of IBA applications and time of taking cutting (February, March, April and May) on the rooting were investigated. The highest rooting percentage (76.67%) in black myrtle was obtained from cuttings taken in April and applied 1000 mg L-1 IBA, while the lowest rooting (10.00%) was obtained from cuttings applied 500 mg L-1 taken in May. White myrtle was rooted at a lower rate than black myrtle. It was determined that the highest rooting percentage (43.33%), shooting percentage (43.33%), rooted-shooted rates (43.33%) and average root number (1.63 pcs) in the white myrtle were 500 mg L-1 IBA dose in April. In addition, the study showed that the best rooting of white myrtle was in the cuttings taken in April and applied 500 mg L-1 IBA. Based on results, it can be concluded that time of taking cuttings in propagation and different concentration of IBA applications had a different impact on the success of black and white myrtle rooting.

Creatinine recovery from bovine urine under the effect of different times and temperatures of storage.

Creatinine is a urinary marker used widely in ruminant's experimental trials. However, despite its great importance no data were found in the literature about the best way to store bovine urine samples. In the sheep urine, was observed an increase in the urinary concentration of creatinine when it was stored acidified (pH 2.5 to 3.5) at a temperature of 28 to 39 °C for 150 days of storage. Nevertheless, urine should be stored acidified (pH below 3) to avoid purine derivative degradation, So, aimed to evaluate creatinine recovery in bovine urine as a function of storage time and temperature. A total of 25 animals' urine (10 Nellore cattle and 15 Holstein cattle) were collected. The urine (40 mL) was diluted in 160 mL of distilled water and its pH was corrected to a value lower than 3 using sulfuric acid drops. A sample of the diluted urine was analyzed to obtain the creatinine concentration reference value on the collection day. The remaining urine was fractionated and preserved at room temperature, cooled (4 °C) or frozen (-20 °C and -40 °C). In the urine of five Holstein cattle was added creatine solutions (20, 40 and 60 mg/dL) to evaluate the creatine to creatinine conservation. These urine samples were analyzed on different days after collection (1, 3, 7, 15, 30 and 45 days). The urine without any added creatine was analyzed on Days 1, 3, 7, 10, 15, 30, 45, 60, 90, 120, and 150 of storage. The addition of creatine in the urine caused an increase in the creatinine concentration (P < 0.05) after 30 days of storage at room temperature and under refrigeration (4 °C). In frozen samples, there was no change in creatinine concentration (P > 0.05). However, creatinine recovery was constant (P > 0.05) until day 15 of storage, regardless of the temperature used, when creatine was not added. After 30 days of storage, an effect of time and/or temperature was observed on creatinine recovery (P < 0.05). Urine samples can be stored at any temperature for up to 15 days after collection to estimate the creatinine concentration. Samples that need storage times longer than 15 days should be frozen (at -20 °C and -40 °C) to avoid creatinine concentration variation.

Combining gas chromatography-ion mobility spectrometry and olfactory analysis to reveal the effect of filled-N2 anaerobic treatment duration on variation in the volatile profiles of gabaron green tea

Gamma-aminobutyric acid green tea (GAGT) is a tea with health benefits by a special anaerobic process to increase the GABA content. However, the effect of this special processing on tea flavor during processing has been rarely reported. In this study, GC-IMS was combined with principal component analysis (PCA) to clarify the effects of different times of anaerobic treatment on the volatile profiles in finished GAGT. GC-O-MS and quantitative descriptive analysis (QDA) were used to investigate the flavor characteristics of GAGT infusion. The results showed that the aroma abundance of GAGT peaked at about 8 h after anaerobic treatment, and then the GAGT flavor delayed. At this point, the differential compounds including nonanal, octanal, heptanal and linalool could be perceived by panalists. Other compounds that didn't change very much in content during processing but had high FD factors, such as β-damascenone, (E)-β-ionone, 1-octen-3-ol and 1-octen-3-one, were also screened as odor-active compounds. Together, they constitute the characteristic flavors of “sweet”, “ketchup” and “slightly fermented” of GAGT infusion. This study will help to better understand the mechanism of GAGT aroma generation and improve the processing.

Water deficit before veraison is crucial in regulating berry VOCs concentration in Sangiovese grapevines.

The impact of water deficit on volatile organic compounds (VOCs) responsible for grape aroma remains quite unclear. The aim of this study was to evaluate the effect of different timing and intensity of water deficit on berry VOCs and on their biosynthetic pathways. Fully irrigated control vines were compared with the following treatments: i) two different levels of water deficit from berry pea-size through veraison, ii) one level of water deficit during the lag-phase, and iii) two different levels of water deficit from veraison through harvest. At harvest, total VOC concentrations were higher in berries of water stressed vines from berry pea size through veraison or during the lag phase, whereas post-veraison water deficit determined similar concentrations as control. This pattern was even more pronounced for the glycosylated fraction and was also observed for single compounds, mainly monoterpenes and C13-norisoprenoids. On the other hand, free VOCs were higher in berries from lag phase or post-veraison stressed vines. The significant glycosylated and free VOCs increment measured after the short water stress limited to the lag phase highlight the pivotal role played by this stage in berry aroma compound biosynthesis modulation. The severity of water stress before veraison was also important, since glycosylated VOCs showed a positive correlation with the pre-veraison daily water stress integral. The RNA-seq analysis showed a wide regulation induced by irrigation regimes on terpenes and carotenoids biosynthetic pathways. The terpene synthases and glycosyltransferases as well as genes of the network of transcription factors were upregulated, especially in berries from pre-veraison stressed vines. Since the timing and intensity of water deficit contribute to regulate berry VOCs, irrigation management can be used to achieve high-quality grapes while saving water.