Effects Of Different Protein Sources Research Articles

Effects of Different Protein Sources on Amino Acid Absorption and Plasma Appearance of Tryptophan, Large Neutral Amino Acids, and Tryptophan Metabolites in Pigs

BackgroundAbsorption of tryptophan (TRP) across the gut epithelium is potentially modulated by competing large neutral amino acids (LNAAs), which could affect the appearance of TRP and its metabolites in the bloodstream. ObjectivesThis study aimed to determine, in a growing pig model of an adult human, the absorption of TRP and other LNAAs from the gastrointestinal tract, and plasma appearance of TRP, LNAAs, and TRP metabolites, in response to dietary proteins varying in TRP content. MethodsPigs were adapted for 7 d to each of 4 diets that differed in their protein source and TRP content: 1) alpha-lactalbumin (AL; 9.95 mg TRP/g diet DM), 2) whey protein (6.59 mg TRP/g), 3) casein (3.73 mg TRP/g), or 4) zein (0.14 mg TRP/g). On day 8, after a 12-h fast, pigs received a test meal consisting of 45 g protein, or a protein-free meal, and were killed 0 (baseline), 1, 2, 3, 4, or 6 h later (n = 6 pigs at each time in each meal group). Tryptophan and LNAA absorption from the small intestine, and appearance of TRP, LNAAs, and TRP metabolites (melatonin, serotonin, kynurenine pathway metabolites), in the portal vein and systemic circulation, were determined. ResultsAL intake resulted in sustained elevated plasma TRP concentrations after an overnight fast. The amount of TRP absorbed was dose-dependently related to protein TRP content (P = 0.028), with fastest rates for pigs fed AL (371 mg/h). Portal and systemic plasma TRP, TRP/LNAA, and the TRP metabolites were highest (P ≤ 0.05) after AL intake, and remained above baseline levels for ∼4 h postprandially. Absorption rates of TRP correlated with postprandial plasma TRP and TRP metabolites (P ≤ 0.05). ConclusionsIn adult humans, postprandial plasma TRP and TRP metabolite concentrations can likely be modulated by the TRP content of the meal.

Growth performance and reproductive qualities of breeder snails (Archachatina marginata) fed different sources of protein

This experiment was conducted to compare the effects of different protein sources on the growth and reproductive performances of breeder snails, as protein sources are the main requirement for fast growth and development of cells for reproduction. Soya bean meal (SBM), cottonseed meal (CSM), and groundnut cake (GNC) were fed to the breeder snails. The snails were eight (8) months old with an initial average weight of 98.93g, and the experiment lasted for 12 weeks. Sixty (60) Archachatina marginata breeder snails were randomly selected and divided into 4 groups of fifteen (15) snails per treatment which were replicated three (3) times in each of the groups. The parameters examined were growth performance, feed intake, feed conversion ratio, and reproductive characteristics. Data collected were analyzed by the use of a one-way analysis of variance and significant means were separated with the Duncan Multiple Ranged Test at a 5% level of probability using SAS, 2011 [10]. The study revealed that soya bean meal (SBM) supports higher final body weight, body weight gain, and percentage fertility, while soya bean meal and cottonseed meal had higher percentage hatchability. However, no significant (P>0.05) differences were recorded in the final shell circumference, shell circumference gain, final shell length, and shell length gain. It was concluded that compounded feed with soya bean meal as the main protein source abetted fast growth and better productive performance.

Effects of different protein sources in low-phosphorus diets on calculated basal endogenous loss of phosphorus by growing pigs

The objective of this experiment was to test the hypothesis that the basal endogenous loss (BEL) of P from pigs fed a diet containing spray dried plasma, casein, or potato protein concentrate is not different from that of pigs fed a diet containing gelatin. Forty pigs (body weight: 19.34 kg; SD = 0.80) were housed individually in metabolism crates and allotted to four low-P diets using a completely randomized block design with two blocks of 20 pigs and five pigs per diet in each block. Diets were based on cornstarch and sucrose and contained 200 g/kg gelatin, 200 g/kg spray dried plasma, 185 g/kg casein, or 200 g/kg potato protein concentrate. With the exception of Ca and P, diets were formulated to meet requirements of all nutrients. Feces and urine samples were collected for 4 d following a 5-d adaptation period. Fecal samples were dried and ground, and fecal and urine samples were analyzed for P. Data were analyzed using a model that included diet as fixed effect and block as random effect. Results indicated that feed intake and fecal excretion of dry matter were greater (P < 0.05) in pigs fed diets containing spray dried plasma, casein, or potato protein concentrate compared with pigs fed the gelatin diet, with pigs fed potato protein concentrate having the greatest (P < 0.05) excretion. The apparent total tract digestibility (ATTD) of dry matter was least (P < 0.001) in pigs fed the diet containing potato protein concentrate, but there were no differences among gelatin, spray dried plasma, and casein diets. The ATTD of P was greater (P < 0.001) in pigs fed diets containing spray dried plasma or casein compared with pigs fed gelatin or potato protein concentrate diets. The BEL of P was not different between the gelatin and casein diets (i.e., 176 and 234 mg/kg dry matter intake), but pigs fed diets containing spray dried plasma or potato protein concentrate had greater (P < 0.001) BEL of P (i.e., 338 and 374 mg/kg dry matter intake) compared with pigs fed gelatin or casein diets. In conclusion, the BEL of P was greater if calculated from diets containing spray dried plasma or potato protein concentrate compared with gelatin. However, casein may be an alternative to gelatin to estimate the BEL of P because casein provides a greater amount of P compared with gelatin, which compensates for the deficient level of P in gelatin, but does not affect the BEL of P.

Cell- and Serum-Derived Proteins Act as DAMPs to Activate RAW 264.7 Macrophage-like Cells on Silicone Implants.

Protein adsorption after biomaterial implantation is the first stage of the foreign body response (FBR). However, the source(s) of the adsorbed proteins that lead to damaged associated molecular patterns (DAMPs) and induce inflammation have not been fully elucidated. This study examined the effects of different protein sources, cell-derived (from a NIH/3T3 fibroblast cell lysate) and serum-derived (from fetal bovine serum), which were compared to implant-derived proteins (after a 30 min subcutaneous implantation in mice) on activation of RAW 264.7 cells cultured in minimal (serum-free) medium. Both cell-derived and serum-derived protein sources when preadsorbed to either tissue culture polystyrene or medical-grade silicone induced RAW 264.7 cell activation. The combination led to an even higher expression of pro-inflammatory cytokine genes and proteins. Implant-derived proteins on silicone explants induced a rapid inflammatory response that then subsided more quickly and to a greater extent than the studies with in vitro cell-derived or serum-derived protein sources. Proteomic analysis of the implant-derived proteins identified proteins that included cell-derived and serum-derived, but also other proteinaceous sources (e.g., extracellular matrix), suggesting that the latter or nonproteinaceous sources may help to temper the inflammatory response in vivo. These findings indicate that both serum-derived and cell-derived proteins adsorbed to implants can act as DAMPs to drive inflammation in the FBR, but other protein sources may play an important role in controlling inflammation.

Effects of Different Protein Sources on Growth Performance, Muscle Flavor Substances and Quality Structure in Triploid Crucian Carp

A 56-day feeding trial was conducted to evaluate the effect of different dietary protein sources on the growth performance, muscle flavor substances, and quality structure of Triploid Crucian Carp. Three isonitrogenous (32.00%), isolipidic (8.00%), and isoenergetic (18.00 MJ kg−1) practical diets were formulated. These diets consisted of fishmeal as the animal-derived protein source (AP), a combination of soybean meal and rapeseed meal as the plant-derived protein source (PP), and a mixture of fishmeal, soybean meal, and rapeseed meal as the mixed protein source (MP). Each diet was randomly assigned to triplicate tanks of fish and each tank was stocked with 25 fish (11.5 ± 0.4 g). The fish were fed until apparent satiation twice a day. The results showed a significant enhancement in the growth performance of Triploid Crucian Carp in the AP group compared with both the MP and PP groups (p &lt; 0.05). Dietary plant derived protein can remarkably reduce the crude lipid content and increase the moisture content of the whole body and the dorsal muscle (p &lt; 0.05). The antioxidant ability of fish in the PP group and MP group was better than that in the AP group (p &lt; 0.05). Regarding free amino acids composition of muscle, the contents of glycine, methionine, and lysine were significantly enhanced in the AP group, while the histidine content was significantly increased in the PP group (p &lt; 0.05). In terms of texture, dietary plant protein significantly improved the muscular hardness, gumminess, and chewiness. The activities of intestinal trypsin and alkaline phosphatase (ALP) of fish in the PP group were significantly higher than that in the AP group (p &lt; 0.05). The expression levels of hepatopancreas TOR and IGF1 genes in the PP group were significantly higher than that in the MP group (p &lt; 0.05). The present results indicate dietary fishmeal significantly improved the growth performance and muscular flavor glycine content, while dietary plant-based protein increased crude protein content, antioxidant ability, and muscular texture performance of Triploid Crucian Carp.

123 Effects of Different Protein Sources Containing Highly Digestible Phosphorus on the Basal Endogenous Loss of Phosphorus

Abstract The objective of this experiment was to test the hypothesis that the basal endogenous loss of P from pigs fed a diet containing blood plasma, casein, or potato protein concentrate is not different from that of pigs fed a diet containing gelatin. Pigs (n = 40; body weigh =: 19.34 kg) were housed in metabolism crates and were randomly allotted to four low-P diets containing 20% gelatin, 20% blood plasma, 18.5% casein, or 20% potato protein concentrate. With the exception of Ca and P, diets were formulated to meet the requirements for 11 to 25 kg pigs. Feces and urine samples were collected separately for 4 d following a 5-d adaptation period. Feces samples were dried, ground, and analyzed for Ca and P, and urine samples were also analyzed for Ca and P. Data were analyzed using a model that included diet as fixed effect and replicate as random effect. Results indicated that feed intake and fecal excretion of dry matter were greater (P &amp;lt; 0.05) in pigs fed diets containing blood plasma, casein, and potato protein concentrate compared with pigs fed the gelatin diet, with pigs fed potato protein concentrate having the greatest (P &amp;lt; 0.05) excretion (Table 1). The apparent total tract digestibility of dry matter was least (P &amp;lt; 0.001) in pigs fed the diet containing potato protein concentrate, but there were no differences among gelatin, blood plasma, and casein diets. No differences were observed in the standardized total tract digestibility of P among diets. Basal endogenous loss of P was not different between the gelatin and casein diets, but pigs fed diets containing blood plasma or potato protein concentrate had greater (P &amp;lt; 0.001) basal endogenous loss of P compared with pigs fed gelatin or casein diets. In conclusion, the basal endogenous loss of P was greater in diets containing blood plasma and potato protein concentrate compared with gelatin. However, casein could be an alternative to gelatin to estimate the basal endogenous loss of P because casein provides a greater amount of P compared with gelatin that compensates for the deficient level of P in gelatin, but does not affect values for the basal endogenous loss of P.

The Effects of Replacing Fish Meal Protein with Black Soldier Fly Meal and Sodium Butyrate Supplementation on the Growth Performance, Lipid Peroxidation, and Intestinal Villi Status of Jade Perch, Scortum barcoo Fingerlings

This study investigated the effects of different protein sources and butyric acid contents in aquafeed on the growth, survival rate, lipid peroxidation enzymes, and intestinal villi of 7.3 g jade perch, Scortum barcoo. The experimental treatment groups were the whole fish meal protein (FM) group, the FM + butyric acid (FMB) group, the 50% fish meal protein and 50% black soldier fly meal protein (FBM) group, the FBM + butyric acid (FBMB) group, the whole black soldier fly meal protein (BM) group, and the BM + butyric acid (BMB) group. The experimental results showed that the jade perch fed with the FMB feed had a significantly higher weight gain rate and antioxidant enzyme activity than the other treatment groups. The red blood cell count, hemoglobin concentration, and hematocrit contents in the blood of jade perch were not affected by the protein source, but they increased with the addition of butyric acid. The intestinal villi length of the jade perch that were fed diets containing butyric acid were significantly higher than that of the jade perch that were fed diets without butyric acid supplementation. The results of this study show that the addition of butyric acid to a balanced feed composed of protein can contribute to the growth of jade perch.

Postprandial effects of dietary protein source on metabolic responses, appetite, and arterial stiffness indices in overweight and obese men: the study protocol for a randomized crossover clinical trial

BackgroundDifferent dietary protein sources are supposed to have various effects on metabolic responses and arterial stiffness in the postprandial period. This study aims to assess the postprandial effects of dietary protein sources, including animal-based protein (AP) and plant-based protein (PP), as part of a high-protein breakfast on appetite response, energy metabolism, and arterial stiffness in overweight and obese men.MethodsThis acute randomized crossover clinical trial will be conducted at the Persian study research center at Imam Reza Hospital, affiliated with the Mashhad University of Medical Sciences, located in the northeast of Iran. Forty-six healthy overweight, and obese men aged 18–60 years will be enrolled based on the eligibility criteria. The subjects will complete two interventions (high-protein AP and PP meals) with 1 week washout period. The primary outcome will be the acute effect of the two test meals on appetite response, energy metabolism parameters, including resting metabolism rate (RMR), diet-induced thermogenesis (DIT), and substrate oxidation (SO), and arterial stiffness indices, including pulse wave velocity (PWV) and pulse wave analysis (PWA). The secondary outcomes include changes in lipemia, glycemia, and insulinemia.DiscussionThe findings of this study will provide novel insight regarding the acute effects of different protein sources on energy metabolism, appetite, and arterial stiffness as a significant cardiovascular disease (CVD) risk factor. It will help dieticians develop effective and efficient meal plans to improve weight reduction and maintenance in overweight/obese individuals.Trial registrationIranian Registry of Clinical Trials; code: IRCT20211230053570N1; registered on February 10, 2022

Effects of different protein sources on nutrient disappearance, rumen fermentation parameters and microbiota in dual-flow continuous culture system

Scarce high-quality protein feed resources has limited the development of animal husbandry. In this study, we used a dual-flow continuous culture system to evaluate effects of difference dietary protein sources including soybean meal (SBM), cottonseed meal (CSM), and rapeseed meal (RSM), on nutrient disappearance, rumen fermentation, and microbiota of XiongDong black goats. Dietary proteins of either CSM, RSM or SBM had no effect on nutrient disappearance (P > 0.05). CSM or RSM significantly reduced (P < 0.01) the pH and enhanced (P < 0.01) the ammonia nitrogen (NH3-N) concentration in fermentation liquid compared to SBM. The short-chain fatty acids (SCFAs) contents were greater (P = 0.05) and acetate was lower (P < 0.01) in SBM than those in RSM and CSM, whereas propionate was greater (P < 0.01) in RSM than that in SBM, consequently reducing the acetate to propionate ratio (A/P) in RSM. Bacteroidetes, Firmicutes, and Proteobacteria were detected as the dominant phyla, and the relative abundances of Spirochaetae (P < 0.01) and Chlorobi (P < 0.05) declined in the CSM and RSM groups as compared to those in the SBM group. At the genus level, Prevotella_1 was the dominant genus; as compared to SBM, its relative abundance was greater (P < 0.01) in CSM and RSM. The abundances of Prevotellaceae_Ga6A1 and Christensenellaceae_R7 were lower (P < 0.05) in CSM, whereas Eubacterium_oxidoreducens_group, and Treponema_2 were lower (P < 0.01) in both CSM and RSM, and other genera were not different (P > 0.10). Although the bacterial community changed with different dietary protein sources, the disappearances of nutrients were not affected, suggesting that CSM and RSM could be used by rumen bacteria, as in case with SBM, and are suitable protein sources for ruminant diets.

Differential effects of various dietary proteins on dextran sulfate sodium-induced colitis in mice.

Chronic colitis is a risk factor for colorectal cancer (CRC) development in both animals and humans. Previously, we reported that a diet rich in protein (with casein as the protein source) significantly increased the risk of mouse CRC development in a dose-dependent manner. In this study, we investigated the effects of different protein sources on the risk of colitis development. Balb/c mice were divided into 7 experimental groups: 20% casein (20C), 20C-dextran sulfate sodium (DSS), 40% casein-DSS (40CD), 40% whey protein-DSS (40WD), 40% soy protein-DSS (40SD), 40% white meat-DSS (40WMD), and 40% red meat-DSS (40RMD). Mice were fed an experimental diet for 4 wk and received 3% DSS in their drinking water for 6 days during the 4th wk of the experimental period. Compared to other groups, the 40CD group showed the most aggravated colitis with increased disease activity and inflammatory markers. In the 40RMD group, interleukin (IL)-6 levels were the highest among all the groups. The 40SD group showed conflicting effects, for example, elevated mortality and disease activity but decreased nitric oxide (NO) levels. The 40WD group showed attenuated colitis with increased IL-10 levels and decreased NO levels. The 40WMD group showed conflicting effects, including decreased NO levels and elevated fecal lipocalin-2 and IL-6 levels. These results suggest that, at levels of 40% in the diet, casein and red meat exacerbate colitis, whereas whey protein mitigates it the most effectively.

Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)

Ghrelin can stimulate pituitary growth hormone (GH) release, and has a key role in the regulation of food intake and adiposity in vertebrates. At present, no relevant studies have reported the effects of Ghrelin on intestinal cell proliferation, protein absorption and metabolism. In this paper, firstly we established the intestinal epithelial cell lines of juvenile grass carp (Ctenopharyngodon idella) that can be passed stably and identified by the intestinal specific expression proteins E-cadherin and ZO-1. And we used this cell line as a model to investigate the effects of Ghrelin, Ala-Gln, Ala-Gln+Ghrelin on the proliferation of juvenile grass carp intestinal cells. We found that the use of Ghrelin, Ala-Gln and Ala-Gln+Ghrelin could promote the proliferation of intestinal cells of juvenile grass carp, but Ala-Gln+Ghrelin had the strongest promoting effect. Further, we investigated the effects of Ghrelin on the expression of cdx2, PepT1 and mTOR which are related to intestinal cell proliferation, protein absorption and metabolism of grass carp in vitro and in vivo experiments. And we studied the effects of different protein sources on the expression of Ghrelin, PepT1 and mTOR in grass carp at individual level. The results suggested that Ghrelin can promote grass carp intestinal cell proliferation, and affect the expression of genes which are related to intestinal peptide absorption and metabolism in juvenile grass carp.

Protein source affects apparent digestibility of feed ingredients and protein metabolism in Chinese perch ( Siniperca chuatsi )

In previous studies of apparent digestibility coefficients (ADCs), the association between apparent digestion and protein metabolism from different protein sources has long been ignored. To develop nutritionally adequate diets, this study evaluated the effects of different protein sources (fermented soybean meal [FSM], rapeseed meal [RSM], Antarctic krill powder [AKP] and fish protein hydrolysate [FPH]) on the growth performance, ADCs and expression of genes related to protein metabolism in Chinese perch. The experimental feed was composed of 300g kg-1 test ingredients and 700g kg-1 reference feed (RF). After the feeding trial, FPH was found to achieve the best growth performance of Chinese perch and had significantly higher ADCs of dry matter and gross energy than all other ingredients. Besides, FPH was the only ingredient with crude protein ADC exceeding 90% and the highest crude lipid ADC, while RSM had the lowest crude protein and crude lipid ADC. FPH showed the highest ADC of amino acids except for serine and tyrosine, and RSM showed the lowest amino acid ADC except for methionine. FPH resulted in the lowest expression of mtor, s6k, murf1, mafbx, pept2 and lat2 and the highest expression of gdh and ast in the muscle. The transcription level of cat significantly increased in the gut of the FSM group compared with that in the RF group. These findings imply that FPH may be a promising ingredient to be incorporated into the formulated diet of Chinese perch.

White meat proteins were more conducive to hepatic antioxidative status than soybean and red meat proteins.

Intake of protein-rich foods might induce oxidative stress in organs. This study investigated the impacts of protein sources including casein, soybean, fish, chicken, pork, and beef proteins on hepatic oxidation and antioxidation status in rats. It was shown that meat proteins produced higher reactive oxygen species in livers (from 64,868 to 87,153F/mgpro) than casein (54,045F/mgpro) and soybean protein (48,361F/mgpro, p<.05). Pork and soybean proteins induced higher hepatic oxidative stress than fish, chicken and beef proteins by increasing malondialdehyde production (>16%, p<.05). White meat (fish and chicken) proteins promoted hepatic superoxide dismutase activity and total antioxidant capacity compared to soybean and red meat (pork and beef) proteins (p<.05). Compared to red meat proteins, white meat proteins increased hepatic expressions of thioredoxin and glutaredoxin. Rats fed red meat proteins showed hepatic steatosis with small vacuoles. Therefore, white meat proteins were more conducive to hepatic antioxidative status than soybean and red meat proteins. PRACTICAL APPLICATIONS: Intake of protein-rich foods may induce oxidative stress response at both cell and organ levels. The objective of this study was to investigate the impacts of different protein sources including casein, soybean, fish, chicken, pork, and beef proteins on oxidation and antioxidation status in rat livers. It was shown that meat proteins induced higher production of reactive oxygen species than casein and soybean protein. However, white meat proteins showed higher antioxidant capacity than soybean and red meat proteins by increasing hepatic superoxide dismutase activity and total antioxidant capacity. Therefore, compared to soybean and red meat proteins, white meat proteins lowered hepatic oxidative stress by reducing malondialdehyde production. This study suggested that intake of moderate white meat proteins was more conducive to hepatic antioxidative status than intake of soybean and red meat proteins. This study would promote the understanding of healthy effects of different protein sources.

Colonic Microbiota and Metabolites Response to Different Dietary Protein Sources in a Piglet Model.

Dietary protein sources have the potential to affect the colon microbiome of piglets that will subsequently have a large impact on metabolic capabilities and hindgut health. This study explored the effects of different protein sources on the growth performance, diarrhea rate, apparent ileal digestibility (AID) of crude protein (CP), colonic mucin chemotypes, colonic microbiome, and microbial metabolites of piglets. Twenty-four piglets were randomly divided into four groups that received isoenergetic and isonitrogenous diets containing either Palbio 50 RD (P50), Soyppt-50% (S50), concentrated degossypolized cottonseed protein (CDCP), or fish meal (FM) as the sole protein source. The experimental diets did not affect the estimated daily gain (EDG), but P50 increased fecal score compared with S50 and CDCP. CDCP increased, but P50 reduced AID of CP in comparison to FM and S50. S50 and CDCP increased the amount of mixed neutral-acidic mucins relative to P50. Venn analysis identified unique OTUs in the P50 (13), CDCP (74), FM (39), and S50 (31) groups. The protein sources did not change the colonic bacterial richness or diversity. High Escherichia abundance in the P50 and FM, great abundant of Lactobacillus in the CDCP, and high Gemmiger abundance in the S50 were found. The CDCP tended to elevate valeric acid and branched chain fatty acid (BCFA) concentrations compared with the other diets. The P50 and FM groups had greater ammonia nitrogen and methylamine contents than the S50 and CDCP groups. There was a positive correlation between the Escherichia and ammonia nitrogen, the Lactobacillus and short chain fatty acid (SCFA), and a negative correlation between the Gemmige and BCFA. These findings suggested short-term feeding of different protein sources did not affect the piglets' growth, but P50 increased the diarrhea rate. Potential pathogenic bacteria and detrimental metabolites appeared in the colons of piglets fed P50 and FM, whereas, beneficial effects were conferred upon piglets fed CDCP and S50, thus indicating that available plant proteins (cotton seed, soy) added to the diets of piglets enhanced colon health by reducing protein fermentation.

Effects of different protein sources completely replacing fish meal in low-protein diet on growth performance, intestinal digestive physiology, and nitrogen digestion and metabolism in nursery pigs.

The study compared the effects of selected proteins replacing fish meal in low-protein diets on piglets' growth performance, intestinal digestive physiology,and nitrogen digestion and metabolism. Five reduced CP, amino acid (AA)-supplemented diets containing 4% of either S50, HP300, concentrated degossypolized cottonseed protein (CDCP), P50, or fish meal were assigned to six pens with 11 pigs for a 28-day study period. Compared with fish meal, dietary proteins did not affect growth performance, apparent total tract digestibility (ATTD) of nutrients, serum hormone levels and biochemical parameters, apparent ileal digestibility (AID) of CP and most AA, colonic short-chain fatty acid (SCFA) contents, duodenal and ileal morphology, digestive enzyme activity, and pH in small intestine of piglets. However, HP300, CDCP, and P50 decreased (p<0.05) fecal N excretion per weight gain. AID of Ile in S50 and HP300 and Glu in P50 were improved (p<0.05), and AID of Gly in other proteins was reduced (p<0.05). S50 and P50 lowered (p<0.05) the contents of colonic isobutyric and isovaleric. S50 and HP300 reduced (p<0.05) jejunal villus height. CDCP increased (p<0.05) the pepsin activity in stomach. S50, HP300, and CDCP decreased (p<0.05) pH in the proximal colon. Overall, the selected proteins could completely replace fish meal in low-protein diet without impairing piglets' growth via maintaining intestinal digestive physiology, andnitrogen digestion and metabolism.

Effects of dietary protein sources on growth performance and feed intake regulation of grass carp (Ctenopharyngodon idellus)

This experiment was conducted to study the effects of different protein sources on growth performance and feed intake regulation of pre-adult grass carp (initial body weight: 153.4 ± 0.3 g), a herbivorous species. Two isonitrogenous, isoenergetic with essential amino acids and available phosphorus balanced diets based on a fish meal (FM) or plant protein blend (PPB) were designed. All fish were fed with FM diet for 4 w to acclimatize to the system and the feeding rhythm. The plasma, hypothalamus, intestinal bulb, and mid-intestine samples were collected at postprandial 3 h (P3h) and 24 h (P24h) after feeding for 7 w. The results showed that final body weight, weight gain rate and specific growth rate of fish in PPB group were significantly higher than those in FM group, and the feed conversion rate was significantly decreased. The dramatic changes in weekly feed intake for PPB group were observed, with significantly lower at 1–3 w, then kept flat at 4 w, and finally higher than that in FM group at 5–7 w. The mRNA levels of feeding regulatory genes in the periphery and central nervous system (CNS) showed that the feeding enhancing genes, ghrelin and AgRP were upregulated, combined with feeding-suppressing genes, like leptin, POMC, PYY were down-regulated in PPB group. Similar trends were validated by metabolic levels, with the increased plasma ghrelin and NPY and the decreased leptin contents in PPB group at P24h. It can be concluded that the differential regulations of appetite factors in periphery and CNS were involved in the reduced appetite of the FM group and actively foraging status of PPB group. Further analyses on taste receptors in the mid-intestine suggested that upregulation of sweet receptors T1R2a was the main reason for the selective preference for the PPB diet after 7 w feeding. Taken together, the feeding habits shift can provide insights into grass carp evolution and vegetarian adaptation.

Effects of Different Protein Sources on Proteolotic Activity in Juveniles of Sterlet, Acipenser ruthenus Linnaeus 1758

Bu çalışmada, çuka balığı, Acipenser ruthenus, larvaları yumurtadan çıkış anından itibaren 29 gün boyunca standart besleme protokolü ile beslenmiştir. Karma yeme geçiş yapılan 29. gündeki proteolitik aktivite ve farklı protein kaynaklarının proteaz aktivite üzerine inhibisyon etkileri ölçülmüştür. Çalışmadan elde edilen bilgiler ışığında, çuka balığı juvenillerinin karma yemlerinde protein kaynağı olarak soya protein konsantresi (%38,57±10,44) ve mısır gluten (%43,07±1,72) kullanmanın, diğer yem hammaddeleri ile karşılaştırıldığında, proteolitik enzim aktivitesini istatistiki açıdan daha olumlu etki yaptığı görülmüştür (P&amp;lt;0,05). Yem hammaddelerinin ikili kombinasyonları incelendiğinde ise, en düşük inhibisyon oranı balık unu: soya protein konsantresinin 1:1 oranda kullanılması (%47,93±1,72) ile elde edilmiş ve bu değerin soya protein konsantresi ve mısır gluteninin tek başlarına kullanılması ile elde edilen etki ile aynı seviyede olduğu tespit edilmiştir (P&amp;gt;0,05). En yüksek inhibe edici etki ise, buğday ile pirinç kepeğinin 3:1 oranda kullanılması ile oluşmuştur (%82,39±7,23).

Animal versus plant protein and adult bone health: A systematic review and meta-analysis from the National Osteoporosis Foundation.

BackgroundProtein may have both beneficial and detrimental effects on bone health depending on a variety of factors, including protein source.ObjectiveThe aim was to conduct a systematic review and meta-analysis evaluating the effects of animal versus plant protein intake on bone mineral density (BMD), bone mineral content (BMC) and select bone biomarkers in healthy adults.MethodsSearches across five databases were conducted through 10/31/16 for randomized controlled trials (RCTs) and prospective cohort studies in healthy adults that examined the effects of animal versus plant protein intake on 1) total body (TB), total hip (TH), lumbar spine (LS) or femoral neck (FN) BMD or TB BMC for at least one year, or 2) select bone formation and resorption biomarkers for at least six months. Strength of evidence (SOE) was assessed and random effect meta-analyses were performed.ResultsSeven RCTs examining animal vs. isoflavone-rich soy (Soy+) protein intake in 633 healthy peri-menopausal (n = 1) and post-menopausal (n = 6) women were included. Overall risk of bias was medium. Limited SOE suggests no significant difference between Soy+ vs. animal protein on LS, TH, FN and TB BMD, TB BMC, and bone turnover markers BSAP and NTX. Meta-analysis results showed on average, the differences between Soy+ and animal protein groups were close to zero and not significant for BMD outcomes (LS: n = 4, pooled net % change: 0.24%, 95% CI: -0.80%, 1.28%; TB: n = 3, -0.24%, 95% CI: -0.81%, 0.33%; FN: n = 3, 0.13%, 95% CI: -0.94%, 1.21%). All meta-analyses had no statistical heterogeneity.ConclusionsThese results do not support soy protein consumption as more advantageous than animal protein, or vice versa. Future studies are needed examining the effects of different protein sources in different populations on BMD, BMC, and fracture.

Proteomic signature of muscle fibre hyperplasia in response to faba bean intake in grass carp

Fish muscle growth is important for the rapidly developing global aquaculture industry, particularly with respect to production and quality. Changes in muscle fibre size are accomplished by altering the balance between protein synthesis and proteolysis. However, our understanding regarding the effects of different protein sources on fish muscle proteins is still limited. Here we report on the proteomic profile of muscle fibre hyperplasia in grass carp fed only with whole faba bean. From the results, a total of 99 significantly changed proteins after muscle hyperplasia increase were identified (p < 0.05, ratio <0.5 or >2). Protein–protein interaction analysis demonstrated the presence of a network containing 56 differentially expressed proteins, and muscle fibre hyperplasia was closely related to a protein–protein network of 12 muscle component proteins. Muscle fibre hyperplasia was also accompanied by decreased abundance in the fatty acid degradation and calcium signalling pathways. In addition, metabolism via the pentose phosphate pathway decreased in grass carp after ingestion of faba bean, leading to haemolysis. These findings could provide a reference for the prevention and treatment of human glucose-6-phosphate dehydrogenase deficiency (“favism”).

ANIMAL VERSUS PLANT PROTEIN INTAKE AND BONE HEALTH‐A SYSTEMATIC REVIEW AND META‐ANALYSIS OF RANDOMIZED CONTROLLED TRIALS

ObjectiveTo conduct a systematic review and meta‐analysis evaluating the effects of animal versus plant protein intake on bone mineral density (BMD), bone mineral content (BMC) and select bone biomarkers in healthy adults. [Registered on International Prospective Register of Systematic Reviews: PROSPERO CRD42015017751].MethodsThis study is a sub‐analysis of a larger systematic review following the methodologies in the Cochrane handbook. Searches across five databases were conducted through June 21, 2016 for randomized controlled trials (RCTs) in healthy adults that examined the effects of animal versus plant protein intake on 1) total body (TB), total hip (TH), lumbar spine (LS) or femoral neck (FN) BMD or TB BMC for at least one year, or 2) select bone formation and resorption biomarkers for at least six months. Random effect meta‐analyses were performed using Stata when data are sufficient.ResultsSeven RCTs were included in qualitative and quantitative synthesis. All included studies were parallel RCTs in healthy peri‐menopausal (n=1 study) and post‐menopausal (n=6) women. All plant protein sources were soy protein; animal protein sources were from milk (n=5), egg white (n=1) or not stated (n=1). Protein interventions ranged from 18–40g. There were no significant differences in BMD or BMC outcomes across studies between soy and animal protein groups, nor when examining collagen type‐1 cross‐linked N‐telopeptide (NTX, n=4)) or deoxypyridinoline (n=1). Though one study found soy protein significantly decreased collagen type‐1 cross‐linked C‐telopeptide (CTx) and bone‐specific alkaline phosphatase (BSAP) compared to milk protein, there were no further studies examining CTx, and BSAP did not significantly differ between groups in other studies (n=4).Meta‐analysis results showed on average, the difference between groups were close to zero and not significant for BMD outcomes (LS: n=4, WMD: 0.001 g/cm2, 95% CI: CI −0.006, 0.009, I2: 2.6%; TB: n=3, WMD: 0.001 g/cm2, 95% CI: −0.005, 0.007, I2: 0.0%; FN: n=3, WMD: −0.000 g/cm2, 95% CI: −0.006, 0.005, I2: 0.0%) and NTx (N=3, WMD: 0.644 nmol BCE, 95% CI −3.004, 4.293, I2: 0.0%;), and there was a nonsignificant, small effect size for BSAP (n=3, SMD: −0.06, 95% CI −0.29, 0.18, I2=0.0%). All outcomes had wide confidence intervals and no to low statistical heterogeneity. While overall risk of bias was low, half of the studies provided inadequate randomization information and had &lt;80% compliance.ConclusionsResults suggest there is no significant difference between plant and animal protein's effects on bone health outcomes in post‐menopausal women. However, studies were limited in number and findings among post‐menopausal women may not be generalizable to other populations. Future studies are needed examining the effects of different protein sources in different populations on BMD, BMC and biomarker outcomes.Support or Funding InformationFunding source: National Osteoporosis Foundation