Effect Of Different Methods Research Articles

Debridement for surgical wounds.

Debridement for surgical wounds.

Identification of horn-derived animal medicines based on detection of keratin-derived characteristic peptides

Due to the highly stable structure of keratin, the extraction and dissolution steps of animal medicines rich in keratin are complex, which seriously restricts the detection efficiency and flux. Therefore, this study simplified the pre-treatment steps of horn samples and optimized the detection methods of characteristic peptides to improve the efficiency of identifying the specificity of horn-derived animal medicines. For detection of the characteristic peptides in horn-derived animal medicines treated with/without iodoace-tamide(IAA), the ion pair conditions of the characteristic peptides were optimized, and the retention time, intensity and other data of the specific peptides were compared between the samples treated with/without IAA. Two pre-treatment methods, direct enzymatic hydrolysis and total protein extraction followed by enzymatic hydrolysis, were used to prepare horn-derived animal medicine samples. The effects of different methods on the detection of specific peptides in the samples of Saiga antelope horn, water buffalo horn, goat horn, and yak horn were compared regarding the retention time of specific peptides and ion intensity. The results indicated that after direct enzymatic hydrolysis, the specific peptides in the samples without IAA treatment can be detected. Compared with the characteristic peptides in the samples treated with IAA, their retention time shifted back and the mass spectrometry response slightly decreased. The specific peptides of the samples without IAA treatment had good specificity and did not affect the specificity identification of horn-derived animal medicines. Overall, the process of direct enzymatic hydrolysis can be used to treat horn samples, omitting the steps of protein extraction and dithiothreitol and IAA treatment, significantly improving the pre-treatment efficiency without affecting the specificity identification of horn-derived animal medicines. This study provides ideas for quality research and standard improvement of horn-derived animal medicines.

Ligamentous Augmentation to Prevent Proximal Junctional Kyphosis and Failure

Study Design: Biomechanical cadaveric study (level V). Objective: To evaluate the effectiveness of polyethylene bands looped around the supra-adjacent spinous process (SP) or spinal lamina (SL) in providing strength to the cephalad unfused segment and reducing junctional stress. Background: Proximal junctional kyphosis (PJK) is a pathologic kyphotic deformity adjacent to posterior spinal instrumentation after fusion constructs. Recent studies demonstrate a mismatch in stiffness between the instrumented construct and nonfused adjacent levels to be a causative factor in the development of PJK and proximal junction failure. To our knowledge, no biomechanical studies have addressed the effect of different methods of polyethylene band placement at the proximal junction. Materials and Methods: Twelve fresh frozen cadavers were divided into 3 groups of 4: pedicle screw-based instrumentation from T10 to L5 (“control”), T10–L5 instrumentation with a polyethylene band to the T9 “SP,” T10–L5 instrumentation with 2 polyethylene bands to the T9 “SL.” Specimens were tested with an eccentric (10 mm anterior) load at 5 mm/min for 15 mm or until failure occurred. Failure was defined by the inflection point on the load versus deformation curves. Linear regression was utilized to evaluate the effect of augmentation on the load-to-failure. Significance was set at 0.05. Results: Fractures occurred in all specimens tested. The mean peak load to failure was 2148 N (974–3322) for the SP group, and 1248 N (742–1754) for the control group (P > 0.05) and 1390 N (1080–2004) for the SL group. No difference existed between the control group and the SP group in terms of fracture level (P > 0.05). Net kyphotic angulation shows no differences among these 3 groups (P > 0.05). Conclusion: Although statistical significance was not achieved, ligament augmentation to the SP increased mean peak load-to-failure in a cadaveric PJK model.

Reviewing The Effectiveness of Different Methods of Applying Post-Tensioned Metal Straps (PTMS) In Enhancing the Flexural Strength of Normal Concrete Beams After Strengthening

Concrete's strength and deformability can be enhanced by adding external lateral confinement. Several materials and confinement techniques have been investigated throughout the development of confined concrete, including Post-Tensioned Metal Straps (PTMS). This study compares three methods of using PTMS to strengthen normal Reinforced Concrete (R.C) beams in bending. First, the method of completely wrapping the beams longitudinally with metal straps is assessed. Secondly, the application of lateral metal straps is examined. Finally, the method of combining metal straps with steel channels is investigated. The assessment criteria included price, effort required for application, applicability of the method, strength enhancement, and ductility of the enhancement. It is concluded that wrapping the beam longitudinally with metal straps is the most cost-effective and easiest method. However, the use of metal straps with steel channels offers greater applicability, strength, and ductility, as it can be applied to any concrete beam. Finally, it is demonstrated that ANSYS can accurately model beams strengthened with metal straps, as evidenced by the good match between the failure modes of beams tested experimentally and analytically.

Production and economic justification of different methods of feeding roughage to calves

The purpose of the research is to study the timing of technological operations and the effect of different methods of feeding roughage to Aberdeen Angus calves on age-related changes in the body weight and the effectiveness of cultivation during the dairy period. 3 groups of newborn Aberdeen Angus calves of 10 heads each were formed: 1st (control) – calves fed roughage together with adult livestock, 2nd (control) – calves fed in a separate pen (canteen) from their mothers, 3rd (control) – calves fed in an experimental feeder with limited access for adult livestock located in a separate pen. For the entire period of research, 201 seconds or 3.35 minutes were spent on performing basic technological operations in the 3rd experimental group, which is 3847 seconds or 64.12 minutes, less than in the 1st group and 609 seconds or 10.15 minutes, less than in the 2nd group. The actual palatability from the experimental feeder (calves of the 3rd group) was higher by 132 kg (p > 0.999) than in the 1st group and by 66 kg (p > 0.99) than in the 2nd group. The smallest amount of uneaten hay residues was in the experimental feeder and amounted to 99 kg which is 54 kg less (p > 0.999), than in the 1st group and 18 kg less than in the 2nd group (p > 0.99). The minimum hay losses were 36 kg in the 3rd group, while in the 1st group this figure was 114 kg, and in the 2nd group 84 kg, which is more by 78 (p > 0.999) and 48 kg (p > 0.999), respectively. At the age of six months, the calves of the 3rd group surpassed the live weight of the herdmates of other groups by 4.2–6.1 kg (p > 0.95–0.99). Their superiority in average daily growth over the entire study period was 21–33 g (p > 0.95–0.99). It turned out to be more cost-effective (by 2.8–4.7 abs. %) to raise the calves which were fed hay from a developed feeder.

Multi-scale effects of behavioral movement deterrents on invasive carp metapopulations

Behavioral deterrents of among-pool movement represent a promising tool for controlling invasive fish populations. To date, much of the research in this area has been focused on the direct effectiveness of different methods of deterrence. However, the effect of these structures on populations in spatially complex habitats is unknown. We combine a metacommunity model with movement data of two invasive species (bighead carp and silver carp) in a large river to assess local and river-wide scale population outcomes of deterrent locations. We calculated (1) which potential deterrent locations are most effective at reducing the growth at the invasion front (2) the river-scale population effects at each location, and (3) what, if any, are the risks imposed by altering the current spatial dynamics. We found that the effects on the population dynamics at the invasion front varied with the location of deterrents, ranging from near zero to effects equal to the reduction in an individual’s movement across the deterrent. The river-scale population growth rate was slightly increased by all potential deterrent placements because the deterrents tended to concentrate more of the river-scale population into pools with the highest recruitment rates. The short-term, transient dynamics followed a strictly decreasing pattern after deterrent placement suggesting no additional short-term risk. These results suggest that deterrents can be an effective and low-risk intervention for the control of invasive carp, although the population level effect will depend on the interaction of the traits and behavior of the species with the physical character and spatial structure of the habitat.

Evaluating the effectiveness of functional decomposition in early-stage design: development and application of problem space exploration metrics

This paper aims to explore metrics for evaluating the effectiveness of functional decomposition methods regarding problem space exploration at the early design stage. Functional decomposition involves breaking down the main purpose of a complex problem or system into a set of more manageable sub-functions, leading to a clearer understanding of the problem space and its various aspects. While various metrics have been used to evaluate functional decomposition outcomes, little literature has focused on assessing its effectiveness in problem space exploration. To address the gap, this research introduces three metrics for problem space evaluation defined by functional models: quantity of unique functions (M1), breadth and depth of the hierarchical structure (M2), and relative semantic coverage ratio of the problem space (M3). An example study is conducted to illustrate the evaluation process, comparing functional analysis with and without explicit human-centric considerations using a power screwdriver as a case product. The analysis in the example study reveals that the breadth of the hierarchical structure (part of M2) is marginally larger in the condition with explicit human-centric considerations (Condition A) compared to the condition without such considerations (Condition B). However, no significant differences are observed in terms of other metrics. The qualitative analysis based on semantic comparisons suggests that Condition A facilitates participants in generating a diverse set of functions supporting user safety requirements more effectively than Condition B. Overall, the example study demonstrates the evaluation process for each metric and discusses their nuances and limitations. By proposing these metrics, this research contributes to benchmarking and evaluating the effectiveness of different methods in promoting functional analysis in engineering design. The metrics provide valuable insights into problem space exploration, offering designers a better understanding of the efficacy of their functional decomposition methods in early design stages. This, in turn, fosters more informed decision-making and contributes to the advancement of functional analysis methodologies in engineering design practices.

Effects of Different Methods of Ovulation Induction on Sex Hormones in Serum, and Meat of Rabbit Does

Effects of Different Methods of Ovulation Induction on Sex Hormones in Serum, and Meat of Rabbit Does

O92: Machine perfusion in liver transplantation; Cochrane review and meta-analysis

Abstract Introduction Several novel machine perfusion technologies have been developed which attempt to improve outcomes compared with ice-box static cold storage (SCS). We aimed to evaluate the effects of different methods of machine perfusion liver transplantation. Methods We used standard, extensive Cochrane search methods to identify randomised machine perfusion trials. Data extraction was performed independently by two authors. Pairwise random-effects meta-analysis was performed. We assessed bias using Risk of Bias 2 and used GRADE to assess certainty of evidence. Results We included seven randomised trials (1024 transplant recipients from 1301 randomised/included livers); four compared end-ischaemic hypothermic oxygenated perfusion (HOPE) with SCS, and three compared normothermic machine perfusion (NMP) with SCS. When compared with SCS, HOPE was associated with improvement in the following clinically relevant outcomes: graft survival (HR=0.45, 95% CI=0.23-0.87; P=0.02; high-certainty evidence), serious adverse events (OR=0.45, 0.22-0.91; P=0.03; moderate-certainty evidence) and clinically significant ischaemic cholangiopathy (OR=0.31, 0.11-0.92; P=0.03; high-certainty evidence). NMP was not associated with improvement in any of these clinically relevant outcomes, although evidence for these outcomes was low certainty. NMP was associated with improved utilisation compared with SCS (one trial found a 50% lower rate of organ discard; P = 0.008). Conclusions Where the decision has been made to transplant a DCD or marginal DBD liver, HOPE will provide superior clinically relevant outcomes compared with SCS alone (graft survival, adverse events, cholangiopathy). NMP appears to improve utilisation of grafts that would otherwise be discarded, but well-powered trials specifically designed to assess utilisation are required.

Analysis of Image Data Augmentation in Smart Agriculture

The demand for agricultural production automation in smart agriculture has made deep learning widely used in tasks, e.g., disease identification, weed-crop segment and fruit detection based on image processing. However, in agricultural production environments, there are limitations in data acquisition, so data enhancement technology has also been widely used. This study introduces two data enhancement methods commonly used in smart agriculture, namely image transformation and GAN-based, and analyzes their specific applications and roles in three common task scenarios: disease identification, weed-crop segment, and fruit detection. According to the analysis, the data enhancement is mainly used to deal with class imbalance problems and over-fitting problems, and can significantly improve model performance in specific scenarios; the complexity of tasks and image structures has an impact on the application effects of different methods, and image segmentation tasks. It is necessary to increase the number of image modes, so methods such as GAN-based are adopted. However, due to the complexity of the image, fruit detection uses simple transformation methods to achieve better results. In addition, data enhancement methods designed for specific tasks, such as simulating synthetic images, can also achieve good results. However, data augmentation technology still has mode collapse problems and is too cumbersome in its application, which points out the direction for subsequent research. Overall, this research sorts out the applications of common data enhancement methods, analyzes the characteristics and limitations of these methods, and hopes to provide guidance for subsequent research.

The Effect of Different Methods of Nano SiO2 coating on Thermoplastic Partial Denture Clasp Materials and Their Effect on Hydrophobicity

Objectives: This in-vitro study aimed to determine the best coating technique of nano SiO2 liquid material and its effect on hydrophobicity of thermoplastic partial denture clasp resins. Materials and methods: 80 thermoplastic disc specimens (Acetal, n=40 and Polyamide, n=40) were fabricated. The specimens were randomly divided into 4 subgroups (n=10/group); brush coating, spray coating, dip coating and uncoated as control group. SEM was used to evaluate the surface topography and the spread of coating material. The subjective method, image quality assessment was used to quantify the images’ quality. The mean contact angle was identified by using Image J software. Results: Mean of opinion score of SEM showed that dip-coated specimens of the two tested materials showed the best coating spread quality among the other coating techniques. Image J software measurements revealed that the contact angle of dip-coated acetal and polyamide specimens exhibited the highest contact angle sequentially in comparison to all tested specimens. Conclusion: These results suggest that the dip coating technique has the best results in comparison with the two other coating techniques. Nano SiO2 coating of thermoplastic clasp materials has improved their contact angle which in turn has improved the hydrophobicity of these clasp materials because their external properties are better than those of control specimens. This study will provide a novel external approach for ameliorating thermoplastic partial denture clasp materials using nano SiO2 to enhance their surface properties and solve their limitations and therefore promote the use of these aesthetic clasps.

Gait retraining targeting foot pronation: A systematic review and meta-analysis.

Foot pronation is a prevalent condition known to contribute to a range of lower extremity injuries. Numerous interventions have been employed to address this issue, many of which are expensive and necessitate specific facilities. Gait retraining has been suggested as a promising intervention for modifying foot pronation, offering the advantage of being accessible and independent of additional materials or specific time. We aimed to systematically review the literature on the effect of gait retraining on foot pronation. We searched four databases including PubMed, Web of Science, Scopus and Embase from their inception through 20 June 2023. The Downs and Black appraisal scale was applied to assess quality of included studies. Two reviewers screened studies to identify studies reporting the effect of different methods of gait-retraining on foot pronation. Outcomes of interest were rearfoot eversion, foot pronation, and foot arch. Two authors separately extracted data from included studies. Data of interest were study design, intervention, variable, sample size and sex, tools, age, height, weight, body mass index, running experience, and weekly distance of running. Mean differences and 95% confidence intervals (CI) were calculated with random effects model in RevMan version 5.4. Fifteen studies with a total of 295 participants were included. The results of the meta-analysis showed that changing step width does not have a significant effect on peak rearfoot eversion. The results of the meta-analysis showed that changing step width does not have a significant effect on peak rearfoot eversion. Results of single studies indicated that reducing foot progression angle (MD 2.1, 95% CI 0.62, 3.58), lateralizing COP (MD -3.3, 95% CI -4.88, -1.72) can effectively reduce foot pronation. Overall, this study suggests that gait retraining may be a promising intervention for reducing foot pronation; Most of the included studies demonstrated significant improvements in foot pronation following gait retraining. Changing center of pressure, foot progression angle and forefoot strike training appeared to yield more favorable outcomes. However, further research is needed to fully understand its effectiveness and long-term benefits.

PEDAGOGICAL APPROACHES TO THE FORMATION OF ECOLOGICAL CONCEPTS

Understanding ecological concepts is crucial for addressing environmental challenges and fostering sustainable behaviors. Pedagogical approaches play a vital role in shaping individuals' comprehension and attitudes towards ecology. This article explores various pedagogical strategies used to enhance the formation of ecological concepts among learners. It examines the effectiveness of different methods, including experiential learning, inquiry-based learning, and outdoor education, in promoting ecological literacy and fostering a deeper connection with the natural world.

Comparative effectiveness of different methods of using the analogue of gonadotropin-releasing hormone for ovulation stimulation in rabbit does

Modern reproductive technologies require artificial insemination (AI) protocols that are safe for the health of animals and do not violate their welfare. Stress factors arising in the reproduction process of industrial rabbit breeding negatively affect the sexual function and reproductive performance of rabbits and their offspring. In recent years, researchers have focused on alternative methods of ovulation stimulation, including intravaginal introduction of hormonal means of its induction. Therefore, the aim of our study was a comparative evaluation of the effectiveness of the intramuscular method of administration of the gonadotropin-releasing control (GnRH) analogue and its addition to the sperm dose for AI on Hyplus rabbit (n = 30), which were randomly divided into three groups. For this, buserelin acetate was administered intramuscularly at a dose of 1 μg per rabbit immediately after artificial insemination (control group). Experimental animals were inseminated with sperm doses with the addition of a GnRH analogue in the amount of 25 μg (experimental group 1) and 15 μg (experimental group 2). The influence of the method of administration of GnRH analogue on reproductive function was evaluated by indicators of the effectiveness of AI, fertility, and the dynamics of the progesterone level during pregnancy. A decrease in fecundity was observed in females; for example, in experimental group 1, the effectiveness of AI in terms of the number of rabbits was 64.3 %; in animals of experimental group 2 – 41.2 %, and the number of inseminations in this group of rabbits was 41.7% higher compared to control data. The fertility of rabbits of all groups was high, reaching the maximum value in the control group (10.42 ± 0.52 heads/female). Fertility according to live newborns in experimental group 1 was the highest among all groups of rabbits and was 9.67 ± 0.49 heads/female, which showed an increasing trend compared to the control. Also, in the animals of experimental group 1, an increase in the level of progesterone in blood serum was observed (on the 7th day of pregnancy by 13.2 %, on the 14th day by 14.3 %, on the 21st day by 16.4 %). In contrast, in experimental group 2, a significant decrease in the progesterone level was noted. Thus, 25 μg of GnRH analogue added to the extender is optimal. It is worth noting that there were no advantages of intravaginal administration over intramuscular administration in terms of reproductive performance. However, the number of live births and progesterone levels were improved.

Effects of different methods of eggshells application on the productivity of sweet basil herb

Sweet basil is very important in the culinary and pharmaceutical sectors because it contains volatile oils that have a variety of biological functions. Considering the economical costs and adverse effects of chemical fertilizers, attention has been turned to the use of ecofriendly and cost effective alternatives. In this regard, eggshells could be used as a natural calcium source for nutring the medicinal and aromatic plants. Therefore, the aim of this trial was to assess the productivity of sweet basil in response to different application methods of eggshells on sandy soil. In this study, eggshells were added to the soil at rates of 1, 2, and 3 g/pot, to foliage at rates of 0.5, 1, and 1.5 g/L, and to irrigation water at rates of 1, 2, and 3 g/L; a rate of 0.5 g/pot of calcium carbonate also used as a reference. Plants exposed to eggshells at 1.5 g/L foliar spray gave the maximum values of fresh and dry weights herb (172.11 and 23.48 g/plant), carbohydrates (36.74%), crude protein (16.56%), inhibition of free radical scavenging (47.61%), total phenols (15.03 mg/g), total flavonoids (2.87 mg/g), and essential oil contents (1.18% or 27.71 g/100 plants). On the other hand, plants subjected to eggshells at 0.5 g/L foliar spray produce the highest amounts of linalool (76.34%) and oxygenated monoterpenes (83.37%). It may be concluded that the morphological and physiological characteristics of sweet basil plant may be enhanced by eggshells.

Seed priming with corona discharge plasma modified growth performance, improved metabolism, and elicited production of tropane alkaloids in Datura inoxia seedlings; plasma technology for application in plant in‐vitro cultures

AbstractThis study monitored the growth, biochemical, and developmental responses of Datura seedlings to the cold plasma. The effectiveness of different methods was explored to break seed dormancy. Germinating seeds were exposed to corona discharge plasma for different durations, including 0, 60, 120, 180, and 300 s. A procedure consisting of illumination with a red light (20 min), soaking in water (4°C for 48 h), and removing a part of Testa was the best method for breaking seed dormancy. The plasma treatments of 60, 120, and 180 s improved the plant growth performance, while this trait was declined in response to the plasma treatment of 300 s. The proline concentrations in both root and leaves displayed a linear significant upward trend in response to the plasma treatments. The plasma for 180 s was the most effective method to increase tropane alkaloids. With increasing the exposure time from 60 to 300 s, the leaf soluble phenols were linearly enhanced. The plasma application for 60, 120, and 180 s significantly augmented total protein concentration, while the exposure of seeds for 300 s significantly diminished it. The highest amounts of photosynthetic pigments (chlorophylls and carotenoids) were recorded in the seedlings treated with plasma for 120 and 180 s. The activities of enzymatic antioxidants (catalase and peroxidase) showed a similar upward trend to that of proline. The plasma priming also improved the phenylalanine ammonia‐lyase activity (a secondary metabolism index). Further investigations are needed to optimize plasma parameters and understand the involved mechanisms to maximize its benefits while minimizing potential risks.

Comparison of Graph Distance Measures for Movie Similarity Using a Multilayer Network Model.

Graph distance measures have emerged as an effective tool for evaluating the similarity or dissimilarity between graphs. Recently, there has been a growing trend in the application of movie networks to analyze and understand movie stories. Previous studies focused on computing the distance between individual characters in narratives and identifying the most important ones. Unlike previous techniques, which often relied on representing movie stories through single-layer networks based on characters or keywords, a new multilayer network model was developed to allow a more comprehensive representation of movie stories, including character, keyword, and location aspects. To assess the similarities among movie stories, we propose a methodology that utilizes a multilayer network model and layer-to-layer distance measures. We aim to quantify the similarity between movie networks by verifying two aspects: (i) regarding many components of the movie story and (ii) quantifying the distance between their corresponding movie networks. We tend to explore how five graph distance measures reveal the similarity between movie stories in two aspects: (i) finding the order of similarity among movies within the same genre, and (ii) classifying movie stories based on genre. We select movies from various genres: sci-fi, horror, romance, and comedy. We extract movie stories from movie scripts regarding character, keyword, and location entities to perform this. Then, we compute the distance between movie networks using different methods, such as the network portrait divergence, the network Laplacian spectra descriptor (NetLSD), the network embedding as matrix factorization (NetMF), the Laplacian spectra, and D-measure. The study shows the effectiveness of different methods for identifying similarities among various genres and classifying movies across different genres. The results suggest that the efficiency of an approach on a specific network type depends on its capacity to capture the inherent network structure of that type. We propose incorporating the approach into movie recommendation systems.

A Novel Common-Mode Voltage Suppression Strategy for Current Source Converter

The problem of common-mode voltage (CMV) in current source converter (CSC) has been concerned with the development and application of CSC, such as in the transformerless motor drive system. Using a common-mode choke is a popular solution to suppressing CMV. The high-frequency harmonics of the CMV can be eliminated by the common-mode filter. However, the low-order harmonics cannot be eliminated effectively, especially at low modulation index, which may cause common-mode resonance and increase the size of the choke. In this paper, a novel virtual space vector modulation (VSVM) method is proposed, which constructs virtual space vectors by the original vectors. The average value of the CMV produced by each virtual vector is zero. The proposed method can reduce the amplitude and third-order harmonic component of the CMV. In addition, the number of switching transitions in a switching sequence of the proposed method is the same as the conventional 5-segment SVM. The suppression effects of different methods on the low-order harmonics of CMV are compared through simulation and experiments, and the effectiveness of the proposed methods is verified.

Anaerobic co-digestion of low Chemical oxygen demand concentration sludge mixture by different pretreatment methods

<p>Three laboratory scale systems were operated to study the anaerobic co-digestion of sewage sludge with the excess activated sludge and cattle manure which contained low COD concentration. The experiments were performed to evaluate the effects of different methods of pretreatment. Since the poor organic substrate, the organic loading rate(OLR) was designed about 1.6kg total solids(TS)(l *d)-1 and the hydraulic retention time was controlled from 78days to 53days throughout the experiment to investigate the effects of the input on overall stability. When the digesters got stabilized, the COD removal efficiencies were 61%, 89% and 54%, respectively, and the pH value fluctuated little in the three digesters. The results indicated that the treatment of 1g volatile solids(VS) of SSEASCM on semi-continuous co-digestion generated more than 200ml of biogas on average. Thus, anaerobic co-digestion of the mixed sludge provided a means for low COD waste treatment and produced renewable energy.</p>

The study of the role of purified anti-mouse CD193 (CCR3) antibody in allergic rhinitis mouse animal models

The pathogenesis of allergic asthma is similar to that of allergic rhinitis, with inflammation cells producing and releasing inflammatory mediators and cytokines closely related to CCR3.Based on the theory of "one airway, one disease", the use of CCR3 monoclonal antibody may have a similar effect on allergic rhinitis. However, there are few studies on CCR3 monoclonal antibody in allergic rhinitis. Therefore, the aim of this study was to investigate the effective concentration of CCR3 monoclonal antibody, to compare the effects of different methods of administration, and to examine the lung condition of allergic mice to investigate whether antibody treatment protects the lungs. In this study, we constructed a mouse model of allergic rhinitis and intraperitoneally injected different doses of CCR3 monoclonal antibody (5, 10, and 20 uL/mg) to observe its therapeutic effect: observing changes in tissue morphology of nasal mucosa, infiltration of inflammation, and using ELISA to detect changes in relevant inflammatory mediators and cytokines, studying the role of CCR3 mAb in inhibiting CCR3-related actions on the nasal mucosa of allergic rhinitis mice. Furthermore, In addition, the therapeutic effects of intraperitoneal injection (i.p.) and intranasal administration (i.n.) were studied on the basis of effective concentrations.