Effects Of Dietary Resveratrol Research Articles

Effects of Dietary Resveratrol and Black Soldier Fly (Hermetia illucens) Larvae Meal Supplements on Quail Egg Production, Quality, and Consumer Acceptance.

This study aimed to examine the effects of supplementing a basal diet with resveratrol and black soldier fly (Hermetiaillucens) larvae meal on Manchurian Golden quail egg production and quality as well as consumer attitudes towards the quail eggs and their acceptability. Quail were allotted three treatments for a laying period of 3 months. The dietary treatment groups were those of a basal diet, a basal diet with 250 mg/kg resveratrol pliusACE, and a diet supplemented with 10% black soldier fly larvae meal (BSF). The BSF larvae meal supplement increased the live weight of the quail, but the differences in the mean egg production and egg external parameters between the experimental groups were insignificant. The egg yolk from the quail fed with resveratrol and BSF supplementation demonstrated lower (p < 0.001 and p < 0.01) pH compared with the quail fed a basal diet. Egg yolk colour showed lower (p < 0.05) lightness and lower (p < 0.01) yellowness and colour saturation in the group of quail fed with resveratrol supplement compared with BSF supplement. Resveratrol inclusion in the basal diet resulted in increased (p < 0.001) protein content in the yolk. BSF inclusion in the diet showed the effect by increasing (p < 0.001) dry matter in albumen. The total monounsaturated fatty acids, polyunsaturated fatty acids, and cholesterol content were not affected by the dietary treatment. The inclusion of BSF larvae meal in the diet increased the proportion of total saturated fatty acids and lowered the proportion of total trans fatty acids in yolk lipids and demonstrated less favourable ratios of fatty acids and nutritional indices, except for the peroxidisability index, and showed higher oxidative status (p < 0.05), flavour score, and overall egg acceptance compared with the quail fed on a basal diet.

The Effects of Dietary Resveratrol and β-Hydroxy-β-Methylbutyric Acid Supplementation at Two Protein Levels on the Ruminal Microbiome and Metabolome of Tibetan Sheep

Resveratrol (RES) and β-hydroxy-β-methylbutyric acid (HMB) have antioxidant, anti-inflammatory, and other beneficial properties. Here, we hypothesize that supplementation with RES and HMB could affect the rumen function in Tibetan sheep. This study aims to explore the effects of RES and HMB supplementation at different protein levels on the rumen microbial and metabolite compositions of Tibetan sheep. Four treatments (n = 30) were prepared according to a 2 × 2 factorial arrangement, with two dietary protein levels (12% and 14%) and two feed additives (RES 1.50 g/day and HMB 1.25 g/day). The experimental treatments were fed diets with 12% CP level non-supplemented (L), 12% protein with RES and HMB (L-RES-HMB), 14% CP level non-supplemented (H), and 14% protein with RES and HMB (H-RES-HMB), respectively. Our results indicated that the trypsin, chymotrypsin, and lipase were significantly increased in the H-RES-HMB group (p &lt; 0.05), while the lipopolysaccharide (LPS) concentration was significantly reduced (p &lt; 0.05). The alpha diversity analysis found that the ACE indices of the L-RES-HMB, and H-RES-HMB groups was significantly higher than that of the L group (p &lt; 0.05). Additionally, compared with the L, L-RES-HMB, and H groups, it was found that the abundance of Euryarchaeota, Spirochaeota, and Metanobrevibacter was significantly increased in the H-RES-HMB group, while the abundance of Proteobacteria was significantly decreased (p &lt; 0.05). A total of 745 significantly different metabolites were identified, of which 14 metabolites were common among the three comparative groups. Differential metabolites were mainly enriched in pathways including the pyrimidine metabolism, the glycine, serine, and threonine metabolisms, and ABC transporters. Overall, CP level and RES/HMB exhibited positively interaction effect on digestive enzyme activity and antioxidant capacity. Dietary RES and HMB supplementation on 14% CP level improved the ruminal digestive enzyme activity and antioxidant capacity through modulating the microbial community and regulating the metabolism.

Effects of Dietary Resveratrol and Curcumin Supplements on Meat Quality and Storage Time in Broilers

Effects of Dietary Resveratrol and Curcumin Supplements on Meat Quality and Storage Time in Broilers

Effects of Dietary Resveratrol, Bile Acids, Allicin, Betaine, and Inositol on Recovering the Lipid Metabolism Disorder in the Liver of Rare Minnow Gobiocypris rarus Caused by Bisphenol A.

The fatty liver is one of the main problems in aquaculture. In addition to the nutritional factors, endocrine disrupter chemicals (EDCs) are one of the causes of fatty liver in fish. Bisphenol A (BPA) is a plasticizer widely used in the production of various plastic products and exhibits certain endocrine estrogen effects. Our previous study found that BPA could increase the accumulation of triglyceride (TG) in fish liver by disturbing the expression of lipid metabolism-related genes. How to recover the lipid metabolism disorder caused by BPA and other environmental estrogens remains to be explored. In the present study, Gobiocypris rarus was used as a research model, and 0.01% resveratrol, 0.05% bile acid, 0.01% allicin, 0.1% betaine, and 0.01% inositol were added to the feed of the G. rarus that exposed to 15 μg/L BPA. At the same time, a BPA exposure group without feed additives (BPA group) and a blank group with neither BPA exposure nor feed additives (Con group) were setted. The liver morphology, hepatosomatic index (HSI), hepatic lipid deposition, TG level, and expression of lipid metabolism-related genes were analyzed after 5 weeks of feeding. The HSI in bile acid and allicin groups was significantly lower than that in Con group. The TG in resveratrol, bile acid, allicin, and inositol groups returned to Con level. Principal component analysis of TG synthesis, decomposition, and transport related genes showed that dietary bile acid and inositol supplementation had the best effect on the recovery of BPA-induced lipid metabolism disorder, followed by allicin and resveratrol. In terms of lipid metabolism-related enzyme activity, bile acid and inositol were the most effective in recovering BPA-induced lipid metabolism disorders. The addition of these additives had a restorative effect on the antioxidant capacity of G. rarus livers, but bile acids and inositol were relatively the most effective. The results of the present study demonstrated that under the present dosage, bile acids and inositol had the best improvement effect on the fatty liver of G. rarus caused by BPA. The present study will provide important reference for solving the problem of fatty liver caused by environmental estrogen in aquaculture.

Resveratrol Attenuates Diquat-Induced Oxidative Stress by Regulating Gut Microbiota and Metabolome Characteristics in Piglets.

Previous studies have shown that dietary resveratrol (RES) reduces diarrhea and attenuates oxidative stress in piglets challenged with diquat. However, the effect of dietary resveratrol on the gut microbiota of these piglets, as well as the potential relationships between intestinal microflora and metabolites, remain unclear. Here, 16S ribosomal DNA sequencing and metabolome analyses were performed to investigate the effect of RES on the gut microbiota and metabolome of diquat-challenged piglets. A total of 18 weaned piglets (aged 28 ± 2 days) were divided into the control group (basal diet), diquat group (basal diet + diquat challenge), and RES group (basal diet containing 90 mg/kg RES + diquat challenge). Compared with the control group, piglets in the diquat group showed enriched relative abundance of the phyla Firmicutes and Actinobacteria, the genus Ruminococcaceae UCG-005, and members of the Eubacterium coprostanoligenes group. Noteworthy, RES supplementation significantly reduced the levels of these microorganisms. In contrast, the relative abundance of some beneficial bacterial species in the RES group, such as the genera Clostridium sensu stricto 1 and Lachnospiraceae unclassified were significantly higher than in the diquat and control groups. Metabolomic analysis indicated that some metabolites, including indole-3-carbinol, 5-hydroxyindole-3-acetic acid, and uridine, were significantly upregulated upon RES supplementation. In particular, the relative abundance of uridine, indole, and alpha- and beta-dihydroresveratrol was significantly higher in the RES group than in the control group. Moreover, most gut bacterial genera were found to be highly correlated with altered gut microbiota-related metabolites. These findings suggest that dietary supplementation with resveratrol may alter the composition and metabolites of colonic microbiota in diquat-challenged piglets, which provides important insights into the use of resveratrol as a feed additive for gut microbial regulation in piglets with inflammatory and oxidative stress-associated disorders.

Protective effects of dietary resveratrol supplementation against oxidative stress in diquat-challenged piglets

To evaluate the protective effects of dietary resveratrol (RES) supplementation against oxidative stress in piglets, a total of 30 weaned piglets (aged 28 + 2 d) were randomly divided into five treatments (n = 6): C group (basal diet), NC group (basal diet + diquat challenge), and basal diet containing either 10, 30, or 90 mg/kg of RES administered to piglets injected with diquat. The trail lasted 21 d. On day 15 after the initiation of treatment, piglets were intraperitoneally injected with 10 mg/kg body weight (BW) diquat or the same amount of saline. The feed intake and faecal consistency were recorded daily. Blood samples were collected for antioxidative, immune parameters and cytokines analysis. RES addition showed a negative tendency (p = .052) on faecal score during the pre-challenge period. Diquat challenge reduced the growth performance and induced oxidative stress and inflammatory responses of piglets (p < .05). However, the growth performance was improved, and faecal score was decreased in 90 mg/kg RES groups (p < .05). Compared with the NC group, addition of 90 mg/kg RES increased activities of superoxide dismutase (SOD), glutathione peroxidase (GSH-Px), and total antioxidant capacity (T-AOC), and reduced malonaldehyde (MDA), cortisol and adrenocorticotropic hormone (ATCH) concentration (p < .05). Moreover, supplementation with 90 mg/kg RES increased serum concentration of interleukin-4 (IL-4) (p < .05) but decreased serum tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6), and interleukin-1β (IL-1β) (p < .05). In conclusion, the study indicated that RES showed overwhelming advantage in reducing diarrhoea and attenuating the oxidative stress of piglets challenged with diquat. Highlights Dietary resveratrol supplementation improved growth performance and reduce diarrhoea of piglets challenged with diquat. The protective effects of dietary resveratrol supplementation against oxidative stress and inflammation in diquat-challenged piglets were confirmed. The experimental results provide theoretical and practical value for further research on the antioxidant mechanisms of resveratrol in weaned piglets.

Effects of Dietary Resveratrol on Gut Microbiota in Mice with Colitis

Accumulating evidence indicated that gut microbiota play important roles in the pathogenesis of colitis, and microbiota composition could be modulated by dietary components. Therefore, ameliorating colitis‐associated bacterial dysbiosis by dietary components may be a unique strategy to improve gut health. Herein, we determined the effects of resveratrol on gut microbiota and their implication in anti‐colonic inflammation in mice with colitis induced by dextran sodium sulfate (DSS). Our results showed that DSS treatment dramatically disturbed the composition of gut microbiota in mice, which was associated with elevated colonic inflammation. Dietary treatment with resveratrol partially attenuated the gut microbiota dysbiosis induced by DSS, for example, resveratrol effectively reduced the abundance of bacteria from Verrucomicrobia and Proteobacteria phyla, decreased the abundance of Sutterella and Bilophila genera, and increased the abundance of Bifidobacterium and Lactobacillus genera in DSS‐treated mice. Alpha and beta‐diversity analysis showed that DSS treatment reduced the microbial diversity and shifted the microbial community structure in comparison with the negative control (healthy) mice, while resveratrol ameliorated the aforementioned alteration and restored the diversity of the gut microbiota in DSS‐treated mice. Furthermore, resveratrol alleviated the symptoms of colitis and decreased the expression levels of pro‐inflammatory cytokines such as IL‐10, IL‐2, GM‐CSF, IL‐1β, IL‐6 and TNF‐α, in the colon of DSS‐treated mice. Overall, our data demonstrated that resveratrol partially reversed bacterial dysbiosis in DSS‐treated mice, which may play an important role in its anti‐colitis effect.Support or Funding InformationThis study was partially supported by fund from USDA

Dietary resveratrol improves immunity but reduces reproduction of broodstock medaka Oryzias latipes (Temminck & Schlegel)

Here, we investigated the effect of dietary resveratrol (20, 40, and 80 µg/g BW/day) on cell-mediated immunity (activity of spleen phagocytes and proliferative response of lymphocytes) and reproductive parameters (egg and sperm quality, i.e. fecundity—total number of eggs produced by individual fish, fertility, embryo survival, and hatching rate) in medaka. Fish fed feed with resveratrol at 40 and 80 µg/g BW/day had significantly higher metabolic activity and intracellular phagocyte killing activity than control. The proliferative lymphocyte activity of the fish from R80 group was greater by more than 20 % in comparison with the control group (P < 0.05). The percentage of macrophages (MO) and their mean fluorescence intensities (MFI) in R40 and R80 groups were significantly higher compared to C and R20 groups (P < 0.05). The differences in MO and MFI values ranged from 52.5 % (±1.5; R0 group) to 65.8 % (±1.6; R80 group) and from 23.2 (±1.4; R0 group) to 38.2 (±2.4; R80 group), respectively. Moreover, resveratrol at 80 µg/g BW/day decreased liver COX activity, i.e. 5.4 in R80 group and 7.9 in R0 group (P < 0.05). The motility parameters of the sperm obtained from the males fed feed supplemented with resveratrol at 80 µg/g BW/day exhibited the highest values except the linearity, which was lower as compared to the control (P < 0.05). The results indicate that diet supplemented with resveratrol at a dosage of 40 µg/g BW/day improves phagocyte killing ability and lymphocyte proliferation in broodstock and accelerates offspring hatch. Also, the results suggest that COX activity influences sperm and oocyte quality in fish; the presence of a COX inhibitor in the dose of 40 µg/g BW/day decreased the embryo survival.

Effects of dietary resveratrol on excess-iron-induced bone loss via antioxidative character

Estrogen deficiency has been considered to be a major cause of osteoporosis, but recent epidemiological evidence and mechanistic studies have indicated that aging and the associated increase in reactive oxygen species (ROS) are the proximal pathogenic factors. Through ROS-mediated reactions, iron can induce disequilibrium of oxidation and antioxidation and can cause bone loss in mice. Therefore, we investigated the effects of resveratrol (RES) on bone mineral density, bone microstructure and the osteoblast functions under iron-overload conditions. Excess iron disrupted the antioxidant/prooxidant equilibrium of the mice and induced the defect and the lesion of the bone trabecula as well as disequilibrium between bone formation and bone resorption in iron-overload mice. Oral administration of RES significantly prevented bone loss in the osteoporotic mice. RES reversed the reduction of Runx2, OCN and type I collagen from excess iron; up-regulated the level of FOXO1; and maintained the antioxidant/prooxidant equilibrium in the mice. RES also reduced the ratio of OPG/RANKL in MC3T3-E1 cells and in mice and significantly inhibited subsequent osteoclastogenesis. These results provide new insights into the antiosteoporosis mechanisms of RES through antioxidative effects, suggesting that RES can be considered a potential natural resource for developing medicines or dietary supplements to prevent and treat osteoporosis.

Effect of dietary resveratrol in ameliorating aflatoxin B1-induced changes in broiler birds.

Consumption of aflatoxin B1 (AFB1) contaminated feed by poultry affects the health of broiler birds causing severe economic losses. The use of phytochemicals is a safe, effective, alternative and practical approach to combat the toxic effect of AF in broilers. Resveratrol, a polyphenol derived from red grapes, berries and peanuts, exerts anti-inflammatory, antioxidant and immunomodulatory effects. Our study was aimed at evaluating the possible protective effects of resveratrol against the adverse effects of AFB1 in broiler birds. A feeding trial of 42 days of duration was undertaken in a completely randomized design with five dietary treatments: G1-AFB1(1.0 ppm); G2-CTR (basal diet alone); G3-AFB1(1.0 ppm)+Resv 0.5%; G4-AFB1(1.0 ppm)+Resv 1%; and G5-Resv 1%. Gain in body weight (BWG) and feed intake (FI) was observed to be highest (p < 0.05) in the AFB1 birds followed by the control group. Feed conversion ratio was lowest in G2-CTR birds and failed to record any significant variation (p > 0.05) between groups as well as within groups. Birds fed resveratrol at both 0.5% and 1.0% levels in combination with AFB1 as well as alone along with basal diet had lower BWG and FI between the fourth and fifth week and also at the fifth week (p < 0.05). No variation (p > 0.05) was obtained in the FCR of AFB1 and resveratrol group of broiler birds. AFB1 feeding significantly increased the activities of aspartate-(AST) and alanine-(ALT) amino transferase, superoxide dismutase (SOD) and catalase (CAT) activities (p < 0.05) but lowered glucose, cholesterol and triglyceride levels in serum. Supplementation of resveratrol helped in increasing the activities of the oxidative enzymes and in improving the plasma total antioxidant capacity (TAOC) and total protein (TP) significantly (p < 0.05) and protein values. The livers of AFB1 group showed degeneration of hepatocytes, bile duct hyperplasia and microgranuloma formation. In resveratrol supplemented birds, the severity and degree of the liver lesions was far less. Apoptotic proteins failed to show any variation in expression between AFB1, control and resveratrol group of birds. The inclusion of resveratrol in broiler diets enhanced antioxidant status of birds indicating the protective effect of resveratrol against AFB1-induced toxicity. So, we advice use of resveratrol as a feed additive to control aflatoxicosis in poultry farms.

Effect of dietary resveratrol on the metabolic profile of nutrients in obese OLETF rats

BackgroundResveratrol (trans-3,4′,5-trihydroxystilbene) is a naturally occurring phytoalexin produced by plants in response to various stresses. Several studies have shown that resveratrol is present in significant amounts in a variety of human diets, including wines, grapes, berries, and peanuts, and it possesses several beneficial health properties, such as atheroprotective, anti-obesity, anti-cancer, anti-inflammatory and antioxidant activities. In this study, we evaluated the effect of resveratrol on the pathogenesis of obesity and the metabolic profile of nutrients in non-high fat-fed obese OLETF rats.ResultsAlthough lipid parameters in the serum and liver were not changed, the accumulation of abdominal white adipose tissues was markedly prevented in resveratrol diet-fed OLETF rats after 4 weeks of feeding. The results of the respiratory gas analysis indicated that dietary resveratrol induced the partial enhancement of fat metabolism and sparing actions for carbohydrate and protein at 1 week and 3 weeks of feeding in OLETF rats. Additionally, the adipose mRNA level of carnitine palmitoyltransferase in the resveratrol diet-fed OLETF rats was higher than the control rats after 4 weeks of feeding.ConclusionOur study demonstrated that dietary resveratrol can prevent obesity through a change in the metabolic profile of nutrients in obese OLETF rats.

Abstract 2142: Dietary grape polyphenol resveratrol regulates Rac activity to increase mammary tumor growth and metastasis

Abstract Resveratrol is a natural compound from grapes and peanuts that is structurally similar to estrogen and can act as an estrogenic or antiestrogenic compound, depending on the concentration. In vitro, high concentrations of resveratrol can have proapoptotic, antigrowth, anti-inflammmatory, antiangiogenic, and anti-invasive properties in cancer cells. However, we reported that resveratrol at low, physiologically relevant, concentrations acts similar to estrogen and promotes breast cancer cell migration via actin cytoskeletal changes regulated by Rac; a small GTPase that plays a pivotal role in cancer cell migration/invasion, and thus, metastasis. Herein, we investigated the effects of dietary resveratrol on mammary tumor growth and metastasis in immunocompromised mouse models of cancer metastasis. Interestingly, our findings suggest promotion of mammary tumor growth and metastasis to lung, liver, and kidney by resveratrol in tumors derived from the low metastatic estrogen receptor (ER)α(−), ERα(+) MDA-MB-231 and the highly metastatic ER(-) MDA-MB-435 cancer cell lines. To determine the role of Rac in resveratrol-mediated mammary tumor progression, the activity of Rac was measured in tumors from resveratrol-treated mice. Our results show a significant increase in tumoral Rac activity, consistent with the concentration of resveratrol that induced the highest metastatic efficiency. Taken together, our findings demonstrate a cancer-promoting role for dietary concentrations of resveratrol in metastatic cancer via Rac activation. Therefore, this study illuminates the importance of further delineating resveratrol's concentration dependent molecular mechanisms, particularly in breast cancer, before it can be tested in the clinic or used as a dietary supplement for cancer patients. This study was supported by DoD/BCRP W81XWH-08-1-0258 to LCP, DoD/BCRP W81XWH-07-1-0330 to SD, the UCC Pilot Project Program, and the UCC Postdoctoral Program sponsored by the PR Science, Technology and Research Trust. The content is solely the responsibility of the authors and does not necessarily represent the official views of the Universidad Central del Caribe or the PR Science, Technology and Research Trust. Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 103rd Annual Meeting of the American Association for Cancer Research; 2012 Mar 31-Apr 4; Chicago, IL. Philadelphia (PA): AACR; Cancer Res 2012;72(8 Suppl):Abstract nr 2142. doi:1538-7445.AM2012-2142

Effects of Dietary Resveratrol on the Sleep-Wake Cycle in the Non-Human Primate Gray Mouse Lemur (Microcebus murinus)*

Converging evidence shows that the non-human primate gray mouse lemur (Microcebus murinus) is ideal for the study of the aging process and for testing the effects of new therapies and dietary interventions on age-associated pathologies. One such dietary supplement is resveratrol (RSV), a dietary polyphenolic compound with several positive effects on metabolic functions and longevity. However, little is known about the effect of RSV on the lemur sleep-wake cycle, which reflects mammalian brain function and health. In the present study, the authors investigated this effect by comparing sleep-wake cycles in adult lemurs based on electroencephalographic (EEG) rhythms. The effect of short-term RSV supplementation on the sleep-wake cycle of mouse lemurs was evaluated in entrained conditions (long-day photoperiods, light:dark 14:10). After 3 wks of RSV supplementation, the animals exhibited a significantly increased proportion of active-wake time, occurring mainly during the resting phase of the sleep-wake cycle (+163%). The increase in active-wake time with RSV supplementation was accompanied by a significant reduction of both paradoxical sleep (−95%) and slow-wave sleep (−38%). These changes mainly occurred during the resting phase of the sleep-wake cycle (RSV supplementation induced negligible changes in active-wake time during the active phase of the sleep-wake cycle). The present data suggest that RSV may be a potent regulator of sleep-wake rhythms and could be of major interest in the study of sleep perturbations associated with aging and neuropathology. (Author correspondence: aujard@mnhn.fr)

Resveratrol protects quail hepatocytes against heat stress: modulation of the Nrf2 transcription factor and heat shock proteins

In the present study, the effects of dietary resveratrol on the induction of heat shock proteins, transcription factors and antioxidative enzyme system in liver of quails under heat stress were investigated. A total of 180 (55-day-old) female Japanese quails (Coturnix coturnix japonica) were reared either at 22 °C for 24 h/day (thermoneutral, TN) or 34 °C for 8 h/day (heat stress, HS; 09:00-17:00 hours) for 12 weeks. Birds in both environments were randomly fed one of three diets: basal diet and basal diet added with either 200 or 400 mg of resveratrol per kg of diet. The results showed that exposure to high ambient temperature induced decreases in feed intake, egg production, and hepatic superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) activities but increases in hepatic malondialdehyde (MDA) concentrations (p < 0.001). Liver Hsp70, Hsp90 and NF-κB expression was greater while Nrf2 expression was lower for quails reared under the heat stress than for those reared under the TN environment (p < 0.0001). There were linear increases in feed intake, egg production, hepatic SOD, CAT, and GSH-Px activities as well as Nrf2 expression, but linear decreases in hepatic MDA concentrations and Hsp70, Hsp90, and NF-κB expressions with increasing supplemental resveratrol level (p < 0.0001). Two-way treatment interactions revealed that the degree of restorations in all response variables was more notable under the high ambient temperature than that of the TN environment as dietary resveratrol concentration was increased. The results of the present study suggest that supplemental resveratrol reduces oxidative stress in heat-stressed quails through modulating the hepatic heat shock proteins and nuclear transcription factors.